BRPI0610201B8 - método para a purificação de anticorpo monoclonal a partir de agregados do mesmo - Google Patents

método para a purificação de anticorpo monoclonal a partir de agregados do mesmo

Info

Publication number
BRPI0610201B8
BRPI0610201B8 BRPI0610201A BRPI0610201A BRPI0610201B8 BR PI0610201 B8 BRPI0610201 B8 BR PI0610201B8 BR PI0610201 A BRPI0610201 A BR PI0610201A BR PI0610201 A BRPI0610201 A BR PI0610201A BR PI0610201 B8 BRPI0610201 B8 BR PI0610201B8
Authority
BR
Brazil
Prior art keywords
aggregates
monoclonal antibody
ion exchange
purifying monoclonal
immunoglobulin
Prior art date
Application number
BRPI0610201A
Other languages
English (en)
Inventor
Kolb Burkard
Sebald Maria
Falkenstein Roberto
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34936911&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI0610201(B8) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of BRPI0610201A2 publication Critical patent/BRPI0610201A2/pt
Publication of BRPI0610201B1 publication Critical patent/BRPI0610201B1/pt
Publication of BRPI0610201B8 publication Critical patent/BRPI0610201B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

método para a purificação de anticorpos. a presente invenção refere-se a método para a purificação de imunoglobulinas através de cromatografia de troca de íon que é descrito. o método cromatográfico usa uma resina de troca de íon fraca e um processo de eluição de etapa única para a purificação de uma imunoglobulina. adicionalmente um método para a determinação da concentração de sal para a eluição de etapa única de uma imunoglobulina a partir de uma resina de troca de íon é descrito.
BRPI0610201A 2005-05-25 2006-05-23 método para a purificação de anticorpo monoclonal a partir de agregados do mesmo BRPI0610201B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP05011302.6 2005-05-25
EP05011302 2005-05-25
PCT/EP2006/004863 WO2006125599A2 (en) 2005-05-25 2006-05-23 Method for the purification of antibodies

Publications (3)

Publication Number Publication Date
BRPI0610201A2 BRPI0610201A2 (pt) 2010-06-01
BRPI0610201B1 BRPI0610201B1 (pt) 2020-12-29
BRPI0610201B8 true BRPI0610201B8 (pt) 2021-05-25

Family

ID=34936911

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0610201A BRPI0610201B8 (pt) 2005-05-25 2006-05-23 método para a purificação de anticorpo monoclonal a partir de agregados do mesmo

Country Status (18)

Country Link
US (3) US20090098660A1 (pt)
EP (2) EP2261252B2 (pt)
JP (1) JP4852602B2 (pt)
KR (1) KR100967778B1 (pt)
CN (1) CN101180317B (pt)
AT (1) ATE525087T1 (pt)
AU (1) AU2006251307B2 (pt)
BR (1) BRPI0610201B8 (pt)
CA (1) CA2607375C (pt)
DK (1) DK1888636T4 (pt)
ES (2) ES2389641T5 (pt)
FI (1) FI1888636T4 (pt)
IL (2) IL186338A (pt)
MX (1) MX2007014269A (pt)
NO (1) NO345362B1 (pt)
PL (1) PL1888636T5 (pt)
TW (2) TWI320788B (pt)
WO (1) WO2006125599A2 (pt)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI320788B (en) 2005-05-25 2010-02-21 Hoffmann La Roche Method for the purification of antibodies
MX2009012786A (es) * 2007-06-01 2009-12-10 Hoffmann La Roche Purificacion de inmunoglobulina.
AR067537A1 (es) * 2007-07-17 2009-10-14 Hoffmann La Roche Purificacion de polipeptidos pegilados
CL2008002054A1 (es) 2007-07-17 2009-05-29 Hoffmann La Roche Metodo para la regeneracion de una columna de cromatografia de intercambio cationico despues de la elusion de eritropoyetina monopeguilada y metodo para obtener una eritropoyetina monopeguilada, incorporando el metodo de regeneracion de la columna de intercambio cationico.
JP5529869B2 (ja) * 2008-08-14 2014-06-25 メルク・シャープ・アンド・ドーム・コーポレーション プロテインａアフィニティークロマトグラフィーを用いる抗体の精製方法
JP5814951B2 (ja) 2010-03-10 2015-11-17 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 免疫グロブリン溶液を精製するための方法
MX2012011737A (es) * 2010-04-14 2012-11-16 Hoffmann La Roche Remocion de agregado de inmunoglobulina.
CA2813747A1 (en) * 2010-10-11 2012-04-19 Chen Wang Processes for purification of proteins
CA2820095C (en) 2010-12-21 2019-02-26 F. Hoffmann-La Roche Ag Isoform enriched antibody preparation and method for obtaining it
WO2012123488A1 (en) * 2011-03-16 2012-09-20 F. Hoffmann-La Roche Ag Ion exchange chromatography with improved selectivity for the separation of polypeptide monomers, aggregates and fragments by modulation of the mobile phase
SG10201605397PA (en) 2011-07-01 2016-08-30 Hoffmann La Roche Method For Separation Of Monomeric Polypeptides From Aggregated Polypeptides
US10481164B2 (en) * 2012-03-26 2019-11-19 Amgen Inc. Method for using light scattering in real time to directly monitor and control impurity removal in purification processes
KR20160054597A (ko) * 2013-09-17 2016-05-16 가부시키가이샤 가네카 신규 항체 정제 방법 및 그로부터 얻어지는 항체, 및 양이온 교환기를 사용한 신규 항체 정제법 및 그로부터 얻어지는 항체
US10351592B2 (en) 2013-10-30 2019-07-16 Celltrion, Inc. Method for separating antibody isoforms using cation exchange chromatography
ES2749383T3 (es) 2014-11-06 2020-03-20 Hoffmann La Roche Variantes de la región Fc con unión al FcRn modificada y procedimientos de uso
GB2539420B (en) * 2015-06-16 2021-01-13 Cytiva Sweden Ab Determination of chromatography conditions
WO2017084738A1 (en) * 2015-11-18 2017-05-26 Merck Patent Gmbh Opposite ph-salt gradients for improved protein separations
AU2016355739A1 (en) * 2015-11-18 2018-07-05 Merck Patent Gmbh Improved protein separation in ion exchange chromatography
CN111902720A (zh) 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
KR20200138346A (ko) * 2018-04-03 2020-12-09 메르크 파텐트 게엠베하 Cex 크로마토그래피 매질 및 생물제약 공급물로부터의 표적 단백질의 저염 용출
CN111068361B (zh) * 2018-10-22 2022-02-11 中国石油化工股份有限公司 一种己内酰胺离子交换装置及其再生方法
CN110066314B (zh) * 2019-04-01 2023-10-27 上海药明生物技术有限公司 一种高效提高多聚体分离分辨率的亲和纯化工艺
TW202128264A (zh) * 2019-09-25 2021-08-01 瑞士商赫孚孟拉羅股份公司 使用經預測之溶離緩衝鹽濃度的陽離子層析法
US20210253631A1 (en) * 2020-01-24 2021-08-19 Alkermes, Inc. Methods of purification
CN115728433A (zh) * 2021-08-27 2023-03-03 联邦生物科技(珠海横琴)有限公司 IgG4型单克隆抗体电荷异质性的离子交换色谱检测方法

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4136094A (en) * 1977-08-31 1979-01-23 The Regents Of The University Of Minnesota Preparation of intravenous human and animal gamma globulins and isolation of albumin
JPS5598117A (en) * 1979-01-17 1980-07-25 Chemo Sero Therapeut Res Inst Purification of gamma-globulin derivative
EP0085747B2 (de) * 1982-02-08 1990-05-30 Schweizerisches Serum- und Impfinstitut und Institut zur Erforschung der Infektionskrankheiten Intravenös verabreichbares humanes Immunglobulin und Verfahren zu dessen Herstellung
JPS6042336A (ja) * 1983-08-18 1985-03-06 Nippon Seiyaku Kk 免疫グロブリンの製造方法
DE3640513A1 (de) 1986-11-27 1988-06-09 Biotest Pharma Gmbh Verfahren zur herstellung eines virussicheren, lagerstabilen und intravenoes vertraeglichen immunglobulin-g-praeparates
US5118796A (en) 1987-12-09 1992-06-02 Centocor, Incorporated Efficient large-scale purification of immunoglobulins and derivatives
US4983722A (en) 1988-06-08 1991-01-08 Miles Inc. Removal of protein A from antibody preparations
US5177194A (en) 1990-02-01 1993-01-05 Baxter International, Inc. Process for purifying immune serum globulins
EP0447585B2 (de) * 1990-03-22 2003-05-07 Biotest Pharma Gmbh Verfahren zur Herstellung eines intravenös verträglichen Immunglobulin-G-Präparates
JP3200850B2 (ja) * 1991-02-26 2001-08-20 味の素株式会社 ヒトｂｃｄｆの精製法
WO1992019973A1 (en) 1991-04-26 1992-11-12 Surface Active Limited Novel antibodies, and methods for their use
DE4118912C1 (pt) 1991-06-08 1992-07-02 Biotest Pharma Gmbh, 6072 Dreieich, De
ES2193136T3 (es) 1991-08-14 2003-11-01 Genentech Inc Variantes de inmunoglubina para receptores especificos de fc epsilon.
EP0576093B1 (en) 1992-06-26 2000-03-22 Association Pour L'essor De La Transfusion Sanguine Dans La Region Du Nord Human monoclonal anti-Rhesus (D) antibodies and cell lines producing same
FR2706466B1 (fr) * 1993-06-14 1995-08-25 Aetsrn Concentré d'immunoglobulines G à usage thérapeutique et procédé de production dudit concentré.
IT1271461B (it) 1993-12-01 1997-05-28 Menarini Ricerche Sud Spa Anticorpo monoclonale bispecifico anti-cd3/anti-egfr,processo per la produzione e suo uso.
DK0659767T4 (da) 1993-12-27 2003-02-10 Zlb Bioplasma Ag Fremgangsmåde til fremstilling af et koncentrat af anti-D-immunoglobulin G og et farmaceutisk præparat, som indeholder dette
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
FI952196A0 (fi) * 1995-05-08 1995-05-08 Suomen Punainen Risti Veripalv Framstaellning av immunoglobulin
IL121900A (en) 1997-10-07 2001-12-23 Omrix Biopharmaceuticals Ltd A method for the purification of immunoglobulins
UA64742C2 (uk) 1997-12-24 2004-03-15 Альфа Терапевтик Корпорейшн СПОСІБ ОДЕРЖАННЯ РОЗЧИНУ <font face="Symbol">g</font>-ГЛОБУЛІНУ, ПРИЗНАЧЕНОГО ДЛЯ ВНУТРІШНЬОВЕННОГО ВВЕДЕННЯ, І ПРОДУКТ, ЩО ОДЕРЖУЄТЬСЯ У ЦЕЙ СПОСІБ (ВАРІАНТИ)
US6162904A (en) * 1997-12-24 2000-12-19 Alpha Therapeutic Corporation Manufacturing method for intraveneously administrable immune globulin and resultant product
WO1999057134A1 (en) † 1998-05-06 1999-11-11 Genentech, Inc. Protein purification by ion exchange chromatography
PT1084136E (pt) * 1998-06-01 2004-12-31 Genentech Inc Separacao de monomeros de anticorpos dos seus multimeros atraves da utilizacao de cromatografia de troca ionica
DK2270044T3 (en) * 1998-06-09 2015-01-26 Csl Behring Ag Liquid immunoglobulin G (IgG) product
AU753468B2 (en) 1998-06-09 2002-10-17 Csl Behring Ag Process for producing immunoglobulins for intravenous administration and other immunoglobulin products
US6441144B1 (en) * 1999-05-20 2002-08-27 Alpha Therapeutic Corporation Method for repairing dual virally inactivated immune globulin for intravenous administration
AU2001249574A1 (en) † 2000-03-29 2001-10-08 Ecokasa Incorporated Nitrogen reduction wastewater treatment system
SE0001128D0 (sv) 2000-03-30 2000-03-30 Amersham Pharm Biotech Ab A method of producing IgG
US6962700B1 (en) * 2000-09-13 2005-11-08 Atopix Pharmaceuticals Corporation Method of manufacturing immune globulin
ES2545067T3 (es) 2002-04-26 2015-09-08 Genentech, Inc. Purificación de proteínas basada en la no afinidad
LT1543038T (lt) 2002-09-11 2017-07-10 Genentech, Inc. Baltymo gryninimas
CN1490334A (zh) * 2002-10-18 2004-04-21 缪金明 基因特异性抗体的亲和纯化方法
PT1601697E (pt) 2003-02-28 2007-09-04 Lonza Biologics Plc Purificação de anticorpos através de proteína a e cromatografia de troca iónica.
AR045614A1 (es) 2003-09-10 2005-11-02 Hoffmann La Roche Anticuerpos contra el recepctor de la interleuquina- 1 y los usos de los mismos
BRPI0510883B8 (pt) * 2004-06-01 2021-05-25 Genentech Inc composto conjugado de droga e anticorpo, composição farmacêutica, método de fabricação de composto conjugado de droga e anticorpo e usos de uma formulação, de um conjugado de droga e anticorpo e um agente quimioterapêutico e de uma combinação
TWI320788B (en) 2005-05-25 2010-02-21 Hoffmann La Roche Method for the purification of antibodies

Also Published As

Publication number Publication date
WO2006125599A3 (en) 2007-06-21
WO2006125599A2 (en) 2006-11-30
AU2006251307A1 (en) 2006-11-30
TWI320788B (en) 2010-02-21
CN101180317B (zh) 2013-06-19
EP2261252B1 (en) 2012-07-25
ES2389641T5 (es) 2021-06-10
EP1888636B2 (en) 2022-03-30
IL186338A (en) 2011-01-31
CA2607375C (en) 2014-01-21
KR20080017322A (ko) 2008-02-26
KR100967778B1 (ko) 2010-07-05
ES2373402T3 (es) 2012-02-03
EP1888636A2 (en) 2008-02-20
EP2261252A2 (en) 2010-12-15
NO20075091L (no) 2008-01-23
PL1888636T3 (pl) 2012-02-29
MX2007014269A (es) 2008-01-24
BRPI0610201A2 (pt) 2010-06-01
CN101180317A (zh) 2008-05-14
TW200946543A (en) 2009-11-16
EP1888636B1 (en) 2011-09-21
AU2006251307B2 (en) 2011-08-04
CA2607375A1 (en) 2006-11-30
CA2607375F (en) 2006-11-30
BRPI0610201B1 (pt) 2020-12-29
ATE525087T1 (de) 2011-10-15
US20090098660A1 (en) 2009-04-16
JP4852602B2 (ja) 2012-01-11
JP2008542218A (ja) 2008-11-27
DK1888636T4 (da) 2022-07-18
TWI391399B (zh) 2013-04-01
FI1888636T4 (fi) 2023-01-31
EP2261252A3 (en) 2011-03-23
US9631007B2 (en) 2017-04-25
TW200716669A (en) 2007-05-01
US11034754B2 (en) 2021-06-15
IL206993A0 (en) 2012-01-01
NO345362B1 (no) 2020-12-28
IL186338A0 (en) 2008-01-20
PL1888636T5 (pl) 2023-03-06
DK1888636T3 (da) 2011-11-14
US20170183394A1 (en) 2017-06-29
ES2389641T3 (es) 2012-10-30
ES2373402T5 (es) 2022-09-14
EP2261252B2 (en) 2020-10-21
US20140243508A1 (en) 2014-08-28

Similar Documents

Publication Publication Date Title
BRPI0610201B8 (pt) método para a purificação de anticorpo monoclonal a partir de agregados do mesmo
ATE363491T1 (de) Reinigung von antikörpern durch protein a und ionenaustauschromatographie
WO2010048192A3 (en) Viral inactivation during purification of antibodies
EA201592192A1 (ru) Способ очистки моноклональных антител
WO2006113643A3 (en) High affinity fully human monoclonal antibodies to interleukin-8 and epitopes for such antibodies
NZ599100A (en) Isolation and purification of anti-il-13 antibodies using protein a affinity chromatography
SE0400501D0 (sv) Antibody purification
NZ592094A (en) Antibodies that bind to il-18 and methods of purifying the same
DE602005027737D1 (de) Verfahren zur antikörpererzeugung
WO2003059935A3 (en) Methods for purifying protein
EP1568710A3 (en) Antibody purification method
ATE384742T1 (de) Verfahren zur reinigung von preproinsulin
DK1472275T3 (da) Proteinoprensning
WO2005090403A3 (en) Method and apparatus for antibody purification
ATE523523T1 (de) Monoklonale anti minichomosome maintenance 2 protein antikörper und verfahren zu deren verwendung beim nachweis einer zervikalen erkrankung
NZ603619A (en) Antibodies that bind to il-12 and methods of purifying the same
WO2011090719A3 (en) Protein purification by ion exchange
CA2589054A1 (en) Detection of a therapeutic antibody in an experimental animal
ATE159532T1 (de) Verfahren zur reinigung von igg-monoklonalen antikörpern
MX2021012251A (es) Metodo para purificar un anticuerpo modificado en la region de fragmento cristalizable (fc).
KR20220079910A (ko) 숙주-세포 단백질을 특성화하는 방법
ATE449177T1 (de) Staphylococcus epidermidis antigene
MX2023011371A (es) Purificacion de anticuerpos mediante cromatografia de modo mezclado.
WO2007011698A3 (en) Humanized antibody conjugates and related methods, assays, reagents, and kits
Pavan et al. Phospho‐l‐tyrosine‐agarose chromatography: Adsorption of human IgG and its proteolytic fragments

Legal Events

Date Code Title Description
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]

Free format text: NOTIFICACAO DE ANUENCIA RELACIONADA COM O ART 229 DA LPI

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 29/12/2020, OBSERVADAS AS CONDICOES LEGAIS.

B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 23/05/2006 OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF