MX2009012786A - Purificacion de inmunoglobulina. - Google Patents
Purificacion de inmunoglobulina.Info
- Publication number
- MX2009012786A MX2009012786A MX2009012786A MX2009012786A MX2009012786A MX 2009012786 A MX2009012786 A MX 2009012786A MX 2009012786 A MX2009012786 A MX 2009012786A MX 2009012786 A MX2009012786 A MX 2009012786A MX 2009012786 A MX2009012786 A MX 2009012786A
- Authority
- MX
- Mexico
- Prior art keywords
- immunoglobulin
- cation exchange
- exchange material
- monomeric
- immunoglobulin purification
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/244—Interleukins [IL]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Oncology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
Abstract
La presente invención se refiere a un método para purificar una inmunoglobulina, en donde el método comprende aplicar una solución amortiguada, acuosa, que comprende una inmunoglobulina en forma monomérica y agregada, a un material de intercambio catiónico bajo condiciones por medio de las cuales la inmunoglobulina en forma monomérica, no se enlaza al material de intercambio catiónico, y recuperar la inmunoglobulina en forma monomérica a partir de la solución después del contacto con el material de intercambio catiónico.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP07010840 | 2007-06-01 | ||
PCT/EP2008/004231 WO2008145351A1 (en) | 2007-06-01 | 2008-05-28 | Immunoglobulin purification |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2009012786A true MX2009012786A (es) | 2009-12-10 |
Family
ID=38440174
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2009012786A MX2009012786A (es) | 2007-06-01 | 2008-05-28 | Purificacion de inmunoglobulina. |
Country Status (14)
Country | Link |
---|---|
US (3) | US9493548B2 (es) |
EP (1) | EP2152745B2 (es) |
JP (1) | JP5064558B2 (es) |
KR (1) | KR101226353B1 (es) |
CN (1) | CN101679509A (es) |
AU (1) | AU2008256411B2 (es) |
BR (1) | BRPI0812288A2 (es) |
CA (1) | CA2687837A1 (es) |
ES (1) | ES2449148T5 (es) |
IL (1) | IL201388A0 (es) |
MX (1) | MX2009012786A (es) |
RU (1) | RU2009148891A (es) |
TW (1) | TWI395609B (es) |
WO (1) | WO2008145351A1 (es) |
Families Citing this family (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2152745B2 (en) * | 2007-06-01 | 2023-03-15 | F. Hoffmann-La Roche AG | Immunoglobulin purification |
CN103396480A (zh) * | 2008-05-15 | 2013-11-20 | 诺沃—诺迪斯克有限公司 | 抗体纯化方法 |
JP2012513425A (ja) * | 2008-12-22 | 2012-06-14 | エフ.ホフマン−ラ ロシュ アーゲー | 免疫グロブリンの精製 |
DK2513134T3 (en) | 2009-12-18 | 2017-12-18 | Novartis Ag | Washing solution and process for affinity chromatography |
EP2695889A1 (en) * | 2009-12-29 | 2014-02-12 | Dr. Reddy's Laboratories Limited | Protein purification by ion exchange |
MX348739B (es) | 2010-03-10 | 2017-06-27 | F Hoffmann-La Roche Ag * | Metodo para purificar soluciones de inmunoglobulina. |
MX2012011737A (es) | 2010-04-14 | 2012-11-16 | Hoffmann La Roche | Remocion de agregado de inmunoglobulina. |
WO2011159074A2 (ko) * | 2010-06-17 | 2011-12-22 | Jeon Sook Yeong | 치료용 면역글로불린 제제 제조장치 및 이를 이용한 제조방법 |
WO2012030512A1 (en) * | 2010-09-03 | 2012-03-08 | Percivia Llc. | Flow-through protein purification process |
EA201300524A1 (ru) | 2010-11-01 | 2013-08-30 | ДСМ АйПи АССЕТС Б.В. | Очистка антител ионообменной хроматографией в едином блоке |
US8911992B2 (en) * | 2011-04-28 | 2014-12-16 | Bio-Rad Laboratories, Inc. | DNA removal in target molecule purification |
IL212911A0 (en) * | 2011-05-16 | 2011-07-31 | Omrix Biopharmaceuticals Ltd | Immunoglobulin reduced in thrombogenic contaminants and preparation thereof |
JP6292718B2 (ja) * | 2011-07-01 | 2018-03-14 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | 凝集ポリペプチドから単量体ポリペプチドを分離するための方法 |
US9096648B2 (en) * | 2011-08-19 | 2015-08-04 | Emd Millipore Corporation | Methods of reducing level of one or more impurities in a sample during protein purification |
PL2773438T5 (pl) | 2011-11-02 | 2022-01-17 | F.Hoffmann-La Roche Ag | Chromatografia przeciążenia i elucji |
SG10201701224UA (en) * | 2012-03-12 | 2017-04-27 | Merck Patent Gmbh | Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode |
ES2381828B1 (es) * | 2012-03-20 | 2012-11-16 | Grifols, S.A. | PROCEDIMIENTO PARA OBTENER UNA COMPOSICION DE IgG MEDIANTE TRATAMIENTO TERMICO |
EP2682168A1 (en) * | 2012-07-02 | 2014-01-08 | Millipore Corporation | Purification of biological molecules |
CN110054661A (zh) | 2013-12-12 | 2019-07-26 | Emd密理博公司 | 使用含丙烯酰胺的过滤器分离蛋白 |
NZ722391A (en) * | 2014-02-04 | 2022-12-23 | Biogen Ma Inc | Use of cation-exchange chromatography in the flow-through mode to enrich post-translational modifications |
CN106414476B (zh) | 2014-03-11 | 2019-12-31 | 株式会社绿十字控股 | 用于纯化免疫球蛋白的方法 |
SI3215528T1 (sl) | 2014-11-06 | 2019-11-29 | Hoffmann La Roche | Variante regije Fc s spremenjeno vezavo FcRn in postopki uporabe |
JPWO2016093251A1 (ja) * | 2014-12-08 | 2017-05-25 | 旭化成メディカル株式会社 | 生理活性物質の精製方法 |
CA3095850A1 (en) * | 2018-04-03 | 2019-10-10 | Merck Patent Gmbh | Cex chromatography media and low salt elution of target proteins from biopharmaceutical feeds |
CN109336970A (zh) * | 2018-11-09 | 2019-02-15 | 杭州奕安济世生物药业有限公司 | 阳离子交换层析法纯化抗体的方法 |
WO2021020474A1 (ja) | 2019-07-31 | 2021-02-04 | 協和キリン株式会社 | 吸着剤を用いた抗体の精製方法 |
Family Cites Families (34)
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US4604208A (en) | 1983-12-29 | 1986-08-05 | Chaokang Chu | Liquid filtration using an anionic microporous membrane |
GB2201904B (en) | 1987-02-14 | 1990-12-19 | Domnick Hunter Filters Ltd | Device for liquid chromatography or immobilised enzyme reaction |
US5118796A (en) | 1987-12-09 | 1992-06-02 | Centocor, Incorporated | Efficient large-scale purification of immunoglobulins and derivatives |
US4889632A (en) | 1987-12-10 | 1989-12-26 | Ceskoslovenska Akademie Ved | Macroporous polymeric membranes for the separation of polymers and a method of their application |
JP3040121B2 (ja) | 1988-01-12 | 2000-05-08 | ジェネンテク,インコーポレイテッド | 増殖因子レセプターの機能を阻害することにより腫瘍細胞を処置する方法 |
US4983722A (en) | 1988-06-08 | 1991-01-08 | Miles Inc. | Removal of protein A from antibody preparations |
ES2074490T5 (es) | 1990-03-22 | 2004-02-16 | Biotest Pharma Gmbh | Procedimiento para la elaboracion de un preparado de inmunoglobulina g tolerable por via intravenosa. |
WO1992015694A1 (en) | 1991-03-08 | 1992-09-17 | The Salk Institute For Biological Studies | Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor |
DE4118912C1 (es) | 1991-06-08 | 1992-07-02 | Biotest Pharma Gmbh, 6072 Dreieich, De | |
CA2116246A1 (en) | 1992-06-26 | 1994-01-06 | Roland Beliard | Human monoclonal anti-rhesus (d) antibodies and cell lines producing same |
IT1271461B (it) | 1993-12-01 | 1997-05-28 | Menarini Ricerche Sud Spa | Anticorpo monoclonale bispecifico anti-cd3/anti-egfr,processo per la produzione e suo uso. |
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ES2228052T3 (es) * | 1998-06-01 | 2005-04-01 | Genentech, Inc. | Separacion demonomeros de anticuerpos de sus multimeros utilizando cromatografia de intercambio de iones. |
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EP3388452A3 (en) | 2002-09-11 | 2019-02-20 | Genentech, Inc. | Protein purification |
GB0304576D0 (en) | 2003-02-28 | 2003-04-02 | Lonza Biologics Plc | Protein a chromatography |
WO2004076485A1 (en) | 2003-02-28 | 2004-09-10 | Lonza Biologics Plc. | Antibody purification by protein a and ion exchange chromatography |
WO2004108260A2 (en) | 2003-05-30 | 2004-12-16 | Advisys, Inc. | Devices and methods for biomaterial production |
SE0400501D0 (sv) * | 2004-02-27 | 2004-02-27 | Amersham Biosciences Ab | Antibody purification |
WO2006024497A1 (en) | 2004-08-30 | 2006-03-09 | Lonza Biologics Plc. | Affinity- plus ion exchange- chromatography for purifying antibodies |
EP1693108A1 (en) | 2004-12-04 | 2006-08-23 | MERCK PATENT GmbH | Mixed-modal anion-exchange type separation material |
TW200902555A (en) | 2005-01-03 | 2009-01-16 | Hoffmann La Roche | Antibodies against IL-13 receptor alpha 1 and uses thereof |
EP1858928A2 (en) | 2005-03-08 | 2007-11-28 | Pharmacia & Upjohn Company LLC | Anti-m-csf antibody compositions having reduced levels of endotoxin |
TWI391399B (zh) | 2005-05-25 | 2013-04-01 | Hoffmann La Roche | 測定溶離多肽之鹽濃度之方法 |
CA2632519A1 (en) * | 2005-12-06 | 2007-07-05 | Amgen Inc. | Polishing steps used in multi-step protein purification processes |
EP2152745B2 (en) * | 2007-06-01 | 2023-03-15 | F. Hoffmann-La Roche AG | Immunoglobulin purification |
US20100069617A1 (en) * | 2008-09-12 | 2010-03-18 | Ge Healthcare Bio-Sciences Ab | Enhanced protein aggregate removal by mixed mode chromatography on hydrophobic interaction media in the presence of protein-excluded zwitterions |
JP6292718B2 (ja) | 2011-07-01 | 2018-03-14 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | 凝集ポリペプチドから単量体ポリペプチドを分離するための方法 |
-
2008
- 2008-05-28 EP EP08758815.8A patent/EP2152745B2/en active Active
- 2008-05-28 CN CN200880018398A patent/CN101679509A/zh active Pending
- 2008-05-28 MX MX2009012786A patent/MX2009012786A/es active IP Right Grant
- 2008-05-28 ES ES08758815T patent/ES2449148T5/es active Active
- 2008-05-28 JP JP2010509727A patent/JP5064558B2/ja active Active
- 2008-05-28 US US12/599,703 patent/US9493548B2/en active Active
- 2008-05-28 AU AU2008256411A patent/AU2008256411B2/en not_active Expired - Fee Related
- 2008-05-28 CA CA002687837A patent/CA2687837A1/en not_active Abandoned
- 2008-05-28 BR BRPI0812288-1A2A patent/BRPI0812288A2/pt not_active IP Right Cessation
- 2008-05-28 KR KR1020097027433A patent/KR101226353B1/ko not_active IP Right Cessation
- 2008-05-28 RU RU2009148891/10A patent/RU2009148891A/ru unknown
- 2008-05-28 WO PCT/EP2008/004231 patent/WO2008145351A1/en active Application Filing
- 2008-05-29 TW TW097119959A patent/TWI395609B/zh not_active IP Right Cessation
-
2009
- 2009-10-11 IL IL201388A patent/IL201388A0/en unknown
-
2016
- 2016-10-19 US US15/298,155 patent/US10590186B2/en active Active
-
2020
- 2020-02-03 US US16/780,382 patent/US11261238B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
WO2008145351A1 (en) | 2008-12-04 |
CN101679509A (zh) | 2010-03-24 |
EP2152745B1 (en) | 2014-01-08 |
BRPI0812288A2 (pt) | 2014-11-25 |
CA2687837A1 (en) | 2008-12-04 |
JP5064558B2 (ja) | 2012-10-31 |
ES2449148T5 (es) | 2023-06-14 |
US20170137497A1 (en) | 2017-05-18 |
RU2009148891A (ru) | 2011-07-20 |
KR20100028064A (ko) | 2010-03-11 |
TW200904503A (en) | 2009-02-01 |
US11261238B2 (en) | 2022-03-01 |
AU2008256411B2 (en) | 2013-08-22 |
JP2010528076A (ja) | 2010-08-19 |
EP2152745A1 (en) | 2010-02-17 |
US10590186B2 (en) | 2020-03-17 |
ES2449148T3 (es) | 2014-03-18 |
US9493548B2 (en) | 2016-11-15 |
AU2008256411A1 (en) | 2008-12-04 |
TWI395609B (zh) | 2013-05-11 |
US20100311952A1 (en) | 2010-12-09 |
IL201388A0 (en) | 2010-05-31 |
US20200377574A1 (en) | 2020-12-03 |
KR101226353B1 (ko) | 2013-01-24 |
EP2152745B2 (en) | 2023-03-15 |
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Legal Events
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