MX348739B - Metodo para purificar soluciones de inmunoglobulina. - Google Patents
Metodo para purificar soluciones de inmunoglobulina.Info
- Publication number
- MX348739B MX348739B MX2012009892A MX2012009892A MX348739B MX 348739 B MX348739 B MX 348739B MX 2012009892 A MX2012009892 A MX 2012009892A MX 2012009892 A MX2012009892 A MX 2012009892A MX 348739 B MX348739 B MX 348739B
- Authority
- MX
- Mexico
- Prior art keywords
- host cell
- protein
- solution
- purifying
- purifying immunoglobulin
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
Abstract
La presente invención se refiere a un método para producir una inmunoglobulina de la subclase IgG1 o IgG4 caracterizado porque comprende las siguientes etapas: i) agregar una solución que consiste de un ácido y agua a un sobrenadante de cultivo de células de mamífero a partir del cual las células de mamífero y los residuos de células de mamífero han sido removidos para ajustar el valor de pH a un valor de pH 5, por lo que a la solución no se le agregan cationes divalentes, ii) incubar el sobrenadante de cultivo de células de mamífero con pH ajustado, y iii) remover el precipitado a partir del sobrenadante de cultivo de células de mamífero incubadas y de esta manera producir la inmunoglobulina, en donde el sobrenadante de cultivo de células de mamífero comprende la inmunoglobulina en una concentración no mayor de 10 mg/ml, en donde la concentración del ácido agregado es 5.5 moles/l o menor y, en donde la incubación es durante 2 horas a 48 horas a un valor de pH de pH 5 a una temperatura de 4°C.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10156103 | 2010-03-10 | ||
| PCT/EP2011/053547 WO2011110598A1 (en) | 2010-03-10 | 2011-03-09 | Method for purifying immunoglobulin solutions |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2012009892A MX2012009892A (es) | 2012-09-12 |
| MX348739B true MX348739B (es) | 2017-06-27 |
Family
ID=42236585
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2012009892A MX348739B (es) | 2010-03-10 | 2011-03-09 | Metodo para purificar soluciones de inmunoglobulina. |
Country Status (12)
| Country | Link |
|---|---|
| US (4) | US9884904B2 (es) |
| EP (1) | EP2545066B1 (es) |
| JP (2) | JP5814951B2 (es) |
| KR (1) | KR101555740B1 (es) |
| CN (1) | CN102791726B (es) |
| BR (1) | BR112012020964B8 (es) |
| CA (1) | CA2789564C (es) |
| ES (1) | ES2648264T3 (es) |
| HK (1) | HK1176946A1 (es) |
| MX (1) | MX348739B (es) |
| RU (1) | RU2558367C2 (es) |
| WO (1) | WO2011110598A1 (es) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5814951B2 (ja) * | 2010-03-10 | 2015-11-17 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 免疫グロブリン溶液を精製するための方法 |
| WO2012149197A2 (en) | 2011-04-27 | 2012-11-01 | Abbott Laboratories | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| SG10201605397PA (en) | 2011-07-01 | 2016-08-30 | Hoffmann La Roche | Method For Separation Of Monomeric Polypeptides From Aggregated Polypeptides |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| US9334319B2 (en) | 2012-04-20 | 2016-05-10 | Abbvie Inc. | Low acidic species compositions |
| WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
| WO2013177118A2 (en) * | 2012-05-21 | 2013-11-28 | Abbvie Inc. | Novel purification of non-human antibodies using protein a affinity chromatography |
| WO2013176754A1 (en) | 2012-05-24 | 2013-11-28 | Abbvie Inc. | Novel purification of antibodies using hydrophobic interaction chromatography |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| KR20150043523A (ko) | 2012-09-02 | 2015-04-22 | 애브비 인코포레이티드 | 단백질 불균일성의 제어 방법 |
| SG11201507230PA (en) | 2013-03-12 | 2015-10-29 | Abbvie Inc | Human antibodies that bind human tnf-alpha and methods of preparing the same |
| WO2014151878A2 (en) | 2013-03-14 | 2014-09-25 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides |
| WO2014159579A1 (en) | 2013-03-14 | 2014-10-02 | Abbvie Inc. | MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| WO2015073884A2 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| KR101917197B1 (ko) * | 2014-03-11 | 2018-11-09 | 주식회사 녹십자홀딩스 | 면역글로불린의 정제방법 |
| SI3215528T1 (sl) | 2014-11-06 | 2019-11-29 | Hoffmann La Roche | Variante regije Fc s spremenjeno vezavo FcRn in postopki uporabe |
| CN111378030A (zh) * | 2018-12-29 | 2020-07-07 | 中国科学院青岛生物能源与过程研究所 | 一种纳米抗体分离纯化的方法 |
| IL292760A (en) * | 2019-11-07 | 2022-07-01 | Janssen Vaccines Prevention B V | Purification of proteins |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5196323A (en) * | 1985-04-27 | 1993-03-23 | Boehringer Ingelheim International Gmbh | Process for preparing and purifying alpha-interferon |
| IL118069A (en) * | 1995-05-02 | 2000-06-01 | Japan Tobacco Inc | Monoclonal antibody reactive to human CETP and assay method for human CETP |
| DE10037781A1 (de) * | 2000-08-03 | 2002-02-21 | Agfa Gevaert Ag | Vorrichtung und Verfahren zum Beschreiben von Aufzeichnungsmaterial mit Bildinformationen |
| ES2184594B1 (es) * | 2001-01-17 | 2004-01-01 | Probitas Pharma Sa | Procedimiento para la produccion de gammaglobulina g humana inactivada de virus. |
| US7332289B2 (en) | 2001-03-09 | 2008-02-19 | Chugai Seiyaku Kabushiki Kaisha | Method of purifying protein |
| ES2626268T3 (es) * | 2002-09-11 | 2017-07-24 | Chugai Seiyaku Kabushiki Kaisha | Método de purificación de proteínas |
| ZA200504990B (en) * | 2003-01-09 | 2006-08-30 | Genentech Inc | Purification of polypeptides |
| CA2885854C (en) | 2004-04-13 | 2017-02-21 | F. Hoffmann-La Roche Ag | Anti-p-selectin antibodies |
| MX2007011735A (es) | 2005-03-24 | 2008-03-14 | Thrombogenics Nv | Anticuerpo anti-plgf novedoso. |
| TWI391399B (zh) | 2005-05-25 | 2013-04-01 | Hoffmann La Roche | 測定溶離多肽之鹽濃度之方法 |
| EP2388274A1 (en) * | 2005-06-17 | 2011-11-23 | Janssen Alzheimer Immunotherapy | Methods of purifying anti A Beta antibodies |
| AR062223A1 (es) | 2006-08-09 | 2008-10-22 | Glycart Biotechnology Ag | Moleculas de adhesion al antigeno que se adhieren a egfr, vectores que los codifican, y sus usos de estas |
| WO2008127305A2 (en) | 2006-11-01 | 2008-10-23 | Biogen Idec Ma Inc. | Method of isolating biomacromolecules using low ph and divalent cations |
| KR101226353B1 (ko) | 2007-06-01 | 2013-01-24 | 에프. 호프만-라 로슈 아게 | 면역글로불린 정제 |
| GB0818228D0 (en) | 2008-10-06 | 2008-11-12 | Avecia Biolog Ltd | Purification process |
| HUE029026T2 (en) | 2009-12-22 | 2017-01-30 | Roche Glycart Ag | Anti-HER3 antibodies and their applications |
| JP5814951B2 (ja) * | 2010-03-10 | 2015-11-17 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 免疫グロブリン溶液を精製するための方法 |
| SG10201605397PA (en) | 2011-07-01 | 2016-08-30 | Hoffmann La Roche | Method For Separation Of Monomeric Polypeptides From Aggregated Polypeptides |
-
2011
- 2011-03-09 JP JP2012556507A patent/JP5814951B2/ja active Active
- 2011-03-09 US US13/582,606 patent/US9884904B2/en active Active
- 2011-03-09 RU RU2012141149/04A patent/RU2558367C2/ru active
- 2011-03-09 CN CN201180013130.3A patent/CN102791726B/zh active Active
- 2011-03-09 KR KR1020127022486A patent/KR101555740B1/ko active IP Right Grant
- 2011-03-09 WO PCT/EP2011/053547 patent/WO2011110598A1/en active Application Filing
- 2011-03-09 MX MX2012009892A patent/MX348739B/es active IP Right Grant
- 2011-03-09 EP EP11707416.1A patent/EP2545066B1/en active Active
- 2011-03-09 CA CA2789564A patent/CA2789564C/en active Active
- 2011-03-09 BR BR112012020964A patent/BR112012020964B8/pt active Search and Examination
- 2011-03-09 ES ES11707416.1T patent/ES2648264T3/es active Active
-
2013
- 2013-04-09 HK HK13104287.9A patent/HK1176946A1/zh unknown
-
2015
- 2015-07-01 JP JP2015132573A patent/JP5931255B2/ja active Active
-
2018
- 2018-02-02 US US15/887,894 patent/US20190010216A1/en not_active Abandoned
-
2019
- 2019-12-19 US US16/721,317 patent/US20200231655A1/en not_active Abandoned
-
2022
- 2022-07-13 US US17/863,587 patent/US20230174625A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| BR112012020964B8 (pt) | 2021-08-24 |
| CN102791726A (zh) | 2012-11-21 |
| JP5814951B2 (ja) | 2015-11-17 |
| HK1176946A1 (zh) | 2013-08-09 |
| US9884904B2 (en) | 2018-02-06 |
| JP2015213511A (ja) | 2015-12-03 |
| US20130005948A1 (en) | 2013-01-03 |
| CN102791726B (zh) | 2016-02-03 |
| JP5931255B2 (ja) | 2016-06-08 |
| EP2545066B1 (en) | 2017-09-20 |
| MX2012009892A (es) | 2012-09-12 |
| WO2011110598A1 (en) | 2011-09-15 |
| KR20120107140A (ko) | 2012-09-28 |
| JP2013521323A (ja) | 2013-06-10 |
| US20200231655A1 (en) | 2020-07-23 |
| KR101555740B1 (ko) | 2015-09-25 |
| RU2012141149A (ru) | 2014-04-20 |
| US20230174625A1 (en) | 2023-06-08 |
| BR112012020964B1 (pt) | 2021-02-23 |
| EP2545066A1 (en) | 2013-01-16 |
| CA2789564A1 (en) | 2011-09-15 |
| BR112012020964A2 (pt) | 2016-05-03 |
| US20190010216A1 (en) | 2019-01-10 |
| RU2558367C2 (ru) | 2015-08-10 |
| ES2648264T3 (es) | 2017-12-29 |
| CA2789564C (en) | 2019-08-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |