MX2018010283A - Metodo para producir factor de crecimiento de hepatocitos (hgf) activo. - Google Patents
Metodo para producir factor de crecimiento de hepatocitos (hgf) activo.Info
- Publication number
- MX2018010283A MX2018010283A MX2018010283A MX2018010283A MX2018010283A MX 2018010283 A MX2018010283 A MX 2018010283A MX 2018010283 A MX2018010283 A MX 2018010283A MX 2018010283 A MX2018010283 A MX 2018010283A MX 2018010283 A MX2018010283 A MX 2018010283A
- Authority
- MX
- Mexico
- Prior art keywords
- hgfa
- growth factor
- activated
- hepatocyte growth
- hgf
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/4753—Hepatocyte growth factor; Scatter factor; Tumor cytotoxic factor II
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21034—Plasma kallikrein (3.4.21.34)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
Abstract
La presente invención proporciona un método para producir activador de factor de crecimiento de hepatocitos activo (HGFA) y factor de crecimiento de hepatocitos activo (HGF) sin usar suero animal. La presente invención se refiere a un método para producir HGFA activo sin usar suero animal. El método se caracteriza porque comprende una etapa de obtener un sobrenadante del cultivo que comprende pro-HGFA, cultivando células de mamífero que expresan activador de factor de crecimiento de hepatocitos inactivo (pro-HGFA) en un medio sin suero, y una etapa de ajustar a débilmente ácido el sobrenadante del cultivo que comprende pro-HGFA obtenido en la etapa anterior para convertir pro-HGFA en HGFA activo. La presente invención se refiere también a un método para producir HGF activo con HGFA producido por el método.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2016054128 | 2016-03-17 | ||
PCT/JP2017/010355 WO2017159722A1 (ja) | 2016-03-17 | 2017-03-15 | 活性型肝細胞増殖因子(hgf)の製造方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2018010283A true MX2018010283A (es) | 2019-01-31 |
Family
ID=59850449
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2018010283A MX2018010283A (es) | 2016-03-17 | 2017-03-15 | Metodo para producir factor de crecimiento de hepatocitos (hgf) activo. |
Country Status (16)
Country | Link |
---|---|
US (2) | US11548926B2 (es) |
EP (1) | EP3431590B1 (es) |
JP (1) | JP6861201B2 (es) |
KR (1) | KR102275262B1 (es) |
CN (1) | CN109196099B (es) |
AU (1) | AU2017232582B2 (es) |
CA (1) | CA3014567C (es) |
DK (1) | DK3431590T3 (es) |
ES (1) | ES2894477T3 (es) |
HU (1) | HUE055414T2 (es) |
IL (1) | IL261066B (es) |
LT (1) | LT3431590T (es) |
MX (1) | MX2018010283A (es) |
RU (1) | RU2747977C2 (es) |
SG (1) | SG11201806843UA (es) |
WO (1) | WO2017159722A1 (es) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11548926B2 (en) | 2016-03-17 | 2023-01-10 | Eisai R&D Management Co., Ltd. | Method for producing an active hepatocyte growth factor (HGF) |
CN110606883A (zh) * | 2019-07-25 | 2019-12-24 | 广州凌腾生物医药有限公司 | 肝细胞生长因子的制备方法 |
CN110607304A (zh) * | 2019-07-25 | 2019-12-24 | 广州凌腾生物医药有限公司 | 肝细胞生长因子的重组表达方法 |
Family Cites Families (43)
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JP2564486B2 (ja) | 1986-07-14 | 1996-12-18 | 修治 橋本 | 肝細胞増殖因子 |
NZ232813A (en) | 1989-03-10 | 1992-08-26 | Snow Brand Milk Products Co Ltd | Human fibroblast glycoprotein, cell differentiation, blood vessel endothelial cell growth factor, cellular immunology inforcing factor of 78 or 74 thousand daltons plus or minus two thousand daltons |
JP2706704B2 (ja) | 1989-06-05 | 1998-01-28 | 敏一 中村 | 組換ヒト肝実質細胞増殖因子 |
JP3036782B2 (ja) | 1990-04-03 | 2000-04-24 | 三菱化学株式会社 | ヒト肝実質細胞増殖因子の生産方法および該因子を産生する形質転換体 |
JP2577091B2 (ja) | 1989-08-11 | 1997-01-29 | 三菱化学株式会社 | 肝実質細胞増殖因子及びそれをコードする遺伝子 |
JP2777678B2 (ja) | 1990-06-11 | 1998-07-23 | 敏一 中村 | 組換ヒト肝実質細胞増殖因子及びその製造方法 |
JP3072628B2 (ja) | 1990-11-19 | 2000-07-31 | 敏一 中村 | ヒト肝実質細胞増殖因子をコードする染色体dna |
JP3213985B2 (ja) * | 1991-10-18 | 2001-10-02 | 三菱化学株式会社 | 新規なタンパク質 |
CA2102463A1 (en) * | 1992-11-05 | 1994-05-06 | Mitsubishi Chemical Corporation | Protein and gene encoding said protein |
JP3232714B2 (ja) * | 1992-11-20 | 2001-11-26 | 三菱化学株式会社 | 新規なタンパク質およびそれをコードする遺伝子 |
JP3698721B2 (ja) | 1993-02-23 | 2005-09-21 | ジェネンテク・インコーポレイテッド | 有機溶媒を用いて処理したポリペプチドの賦形剤安定化 |
JP2577091Y2 (ja) | 1993-06-23 | 1998-07-23 | 賢一 片山 | 分流装置の合成樹脂製サドル |
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JP3927248B2 (ja) | 1995-07-11 | 2007-06-06 | 第一製薬株式会社 | Hgf凍結乾燥製剤 |
JP2859577B2 (ja) | 1996-03-27 | 1999-02-17 | 三菱化学株式会社 | 肝実質細胞増殖因子 |
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JP2980888B2 (ja) | 1998-07-09 | 1999-11-22 | 三菱化学株式会社 | 肝実質細胞増殖因子をコードする遺伝子 |
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JP4090236B2 (ja) * | 2000-12-05 | 2008-05-28 | 三菱化学株式会社 | 活性型肝細胞増殖因子アクティベーターに対する特異的抗体とその使用法 |
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RU2506946C1 (ru) | 2012-11-28 | 2014-02-20 | Государственное бюджетное образовательное учреждение высшего профессионального образования "Воронежская государственная медицинская академия им. Н.Н. Бурденко" Министерства здравоохранения и социального развития Российской Федерации | Технология лиофилизации обогащенной тромбоцитами плазмы с сохранением жизнеспособности факторов tgf pdgf vegf |
ES2887079T3 (es) | 2014-04-28 | 2021-12-21 | Eisai R&D Man Co Ltd | Preparación de HGF liofilizada |
US11548926B2 (en) | 2016-03-17 | 2023-01-10 | Eisai R&D Management Co., Ltd. | Method for producing an active hepatocyte growth factor (HGF) |
US20200341014A1 (en) | 2016-03-18 | 2020-10-29 | Eisai R&D Management Co., Ltd. | PD Marker of Hepatocyte Growth Factor (HGF) |
CN208371461U (zh) | 2017-08-21 | 2019-01-15 | 潍坊鸿旺工贸有限公司 | 一种晾衣架用连接构件及晾衣架 |
-
2017
- 2017-03-15 US US16/078,568 patent/US11548926B2/en active Active
- 2017-03-15 EP EP17766718.5A patent/EP3431590B1/en active Active
- 2017-03-15 DK DK17766718.5T patent/DK3431590T3/da active
- 2017-03-15 RU RU2018130530A patent/RU2747977C2/ru active
- 2017-03-15 JP JP2018505972A patent/JP6861201B2/ja active Active
- 2017-03-15 CA CA3014567A patent/CA3014567C/en active Active
- 2017-03-15 SG SG11201806843UA patent/SG11201806843UA/en unknown
- 2017-03-15 ES ES17766718T patent/ES2894477T3/es active Active
- 2017-03-15 CN CN201780013057.7A patent/CN109196099B/zh active Active
- 2017-03-15 WO PCT/JP2017/010355 patent/WO2017159722A1/ja active Application Filing
- 2017-03-15 AU AU2017232582A patent/AU2017232582B2/en active Active
- 2017-03-15 LT LTEPPCT/JP2017/010355T patent/LT3431590T/lt unknown
- 2017-03-15 MX MX2018010283A patent/MX2018010283A/es unknown
- 2017-03-15 HU HUE17766718A patent/HUE055414T2/hu unknown
- 2017-03-15 KR KR1020187024470A patent/KR102275262B1/ko active IP Right Grant
-
2018
- 2018-08-08 IL IL261066A patent/IL261066B/en unknown
-
2022
- 2022-05-05 US US17/737,343 patent/US20220267393A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
AU2017232582A1 (en) | 2018-08-30 |
US20190062390A1 (en) | 2019-02-28 |
IL261066B (en) | 2021-08-31 |
SG11201806843UA (en) | 2018-09-27 |
US20220267393A1 (en) | 2022-08-25 |
EP3431590A1 (en) | 2019-01-23 |
ES2894477T3 (es) | 2022-02-14 |
RU2018130530A3 (es) | 2020-08-13 |
KR20180127322A (ko) | 2018-11-28 |
RU2747977C2 (ru) | 2021-05-18 |
AU2017232582B2 (en) | 2022-07-14 |
KR102275262B1 (ko) | 2021-07-12 |
JPWO2017159722A1 (ja) | 2019-01-24 |
IL261066A (en) | 2018-10-31 |
EP3431590A4 (en) | 2019-11-20 |
DK3431590T3 (da) | 2021-10-11 |
HUE055414T2 (hu) | 2021-11-29 |
EP3431590B1 (en) | 2021-07-28 |
WO2017159722A1 (ja) | 2017-09-21 |
CN109196099B (zh) | 2022-01-11 |
RU2018130530A (ru) | 2020-04-17 |
US11548926B2 (en) | 2023-01-10 |
LT3431590T (lt) | 2021-09-10 |
CA3014567C (en) | 2024-02-20 |
CA3014567A1 (en) | 2017-09-21 |
CN109196099A (zh) | 2019-01-11 |
JP6861201B2 (ja) | 2021-04-21 |
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