MX2016013223A - Formulacion en polvo de medio de compuesto y metodo de preparacion de medio liquido para cultivo celular. - Google Patents

Formulacion en polvo de medio de compuesto y metodo de preparacion de medio liquido para cultivo celular.

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Publication number
MX2016013223A
MX2016013223A MX2016013223A MX2016013223A MX2016013223A MX 2016013223 A MX2016013223 A MX 2016013223A MX 2016013223 A MX2016013223 A MX 2016013223A MX 2016013223 A MX2016013223 A MX 2016013223A MX 2016013223 A MX2016013223 A MX 2016013223A
Authority
MX
Mexico
Prior art keywords
cell culture
powder formulation
culture medium
compounded
preparation
Prior art date
Application number
MX2016013223A
Other languages
English (en)
Inventor
Shimoni Yuval
Moehrle Volker
Original Assignee
Bayer Healthcare Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Healthcare Llc filed Critical Bayer Healthcare Llc
Publication of MX2016013223A publication Critical patent/MX2016013223A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0031—Serum-free culture media
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
    • G01N31/221—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators for investigating pH value
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/35—Polyols, e.g. glycerin, inositol
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/42—Organic phosphate, e.g. beta glycerophosphate
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención proporciona una formulación en polvo de medio de cultivo celular compuesto que comprende: un polvo de medio basal y un complemento de medio de cultivo celular, en el que el complemento de medio de cultivo celular comprende y una o más sales; uno o más factores de crecimiento; uno o más iones inorgánicos; un complemento de aminoácidos que comprende uno o más de asparagina, glutamina, histidina, y serina; uno o más reguladores; y uno o más agentes antiespumantes. La invención adicionalmente proporciona métodos para elaborar una formulación en polvo de medio de cultivo celular compuesto, métodos para elaborar un medio de cultivo celular para hacer crecer células de mamíferos y métodos para producir una proteína de interés al cultivar células en el medio de cultivo celular y aislar la proteína de interés.
MX2016013223A 2014-04-10 2015-04-07 Formulacion en polvo de medio de compuesto y metodo de preparacion de medio liquido para cultivo celular. MX2016013223A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461978027P 2014-04-10 2014-04-10
PCT/US2015/024780 WO2015157335A1 (en) 2014-04-10 2015-04-07 Compounded media powder formulation and method of preparation of liquid medium for cell culture

Publications (1)

Publication Number Publication Date
MX2016013223A true MX2016013223A (es) 2017-04-27

Family

ID=53055095

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016013223A MX2016013223A (es) 2014-04-10 2015-04-07 Formulacion en polvo de medio de compuesto y metodo de preparacion de medio liquido para cultivo celular.

Country Status (14)

Country Link
US (1) US10227559B2 (es)
EP (1) EP3129463B1 (es)
JP (1) JP6665104B2 (es)
KR (1) KR20160144429A (es)
CN (1) CN106459907B (es)
AU (1) AU2015243961A1 (es)
CA (1) CA2945390A1 (es)
IL (1) IL248218A0 (es)
MX (1) MX2016013223A (es)
PE (1) PE20161326A1 (es)
RU (1) RU2016144057A (es)
SG (1) SG11201608390SA (es)
WO (1) WO2015157335A1 (es)
ZA (1) ZA201607719B (es)

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CN106011071B (zh) * 2016-08-09 2019-03-01 海南海医药物安全性评价研究有限责任公司 一种原代肿瘤细胞培养组合物及其应用
KR102216302B1 (ko) * 2018-09-21 2021-02-17 메디포스트(주) 피부 세포 활성화용 조성물 및 이의 용도
CN109628377A (zh) * 2019-01-02 2019-04-16 贵州省人民医院 一种小鼠原代肝细胞灌注式分离及离体培养方法
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EP3889251A1 (en) * 2020-03-31 2021-10-06 Fundació Centre de Regulació Genòmica Culture media for mycoplasma

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Also Published As

Publication number Publication date
SG11201608390SA (en) 2016-11-29
PE20161326A1 (es) 2016-12-11
CN106459907A (zh) 2017-02-22
KR20160144429A (ko) 2016-12-16
US20170191025A1 (en) 2017-07-06
JP2017510283A (ja) 2017-04-13
CN106459907B (zh) 2020-11-06
AU2015243961A1 (en) 2016-10-27
EP3129463B1 (en) 2021-05-26
IL248218A0 (en) 2016-11-30
RU2016144057A3 (es) 2018-11-22
RU2016144057A (ru) 2018-05-15
WO2015157335A1 (en) 2015-10-15
EP3129463A1 (en) 2017-02-15
JP6665104B2 (ja) 2020-03-13
ZA201607719B (en) 2018-11-28
CA2945390A1 (en) 2015-10-15
US10227559B2 (en) 2019-03-12

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