MX2018013469A - Medio enriquecido con nutrientes para el crecimiento de celulas derivadas de tejido de cordon umbilical humano. - Google Patents

Medio enriquecido con nutrientes para el crecimiento de celulas derivadas de tejido de cordon umbilical humano.

Info

Publication number
MX2018013469A
MX2018013469A MX2018013469A MX2018013469A MX2018013469A MX 2018013469 A MX2018013469 A MX 2018013469A MX 2018013469 A MX2018013469 A MX 2018013469A MX 2018013469 A MX2018013469 A MX 2018013469A MX 2018013469 A MX2018013469 A MX 2018013469A
Authority
MX
Mexico
Prior art keywords
serum
enriched media
hutc
nutrient enriched
transferrin
Prior art date
Application number
MX2018013469A
Other languages
English (en)
Inventor
Ravinder Bhatia
L S Klaudyne Hong
Ozturk Sadettin S
Venkat H Kamaraju
Original Assignee
Depuy Synthes Products Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Depuy Synthes Products Inc filed Critical Depuy Synthes Products Inc
Publication of MX2018013469A publication Critical patent/MX2018013469A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Esta invencion proporciona metodos de cultivar celulas dependientes de anclaje (por ejemplo, hUTC) en medio de cultivo que comprende aminoacidos, vitaminas, sales, nucleosidos, insulina, transferrina, etanolamina y sodio selenio, en donde el medio de cultivo se complementa con suero; el metodo comprende, ademas, la adicion de una solucion nutriente libre de suero que comprende aminoacidos, vitaminas, sales, nucleosidos, insulina, transferrina, etanolamina y sodio selenio; la invencion proporciona, ademas, un medio de cultivo y soluciones nutrientes libre de suero para el cultivo de celulas dependientes de anclaje.
MX2018013469A 2012-12-14 2015-06-12 Medio enriquecido con nutrientes para el crecimiento de celulas derivadas de tejido de cordon umbilical humano. MX2018013469A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13/715,532 US20140170748A1 (en) 2012-12-14 2012-12-14 Nutrient Enriched Media for hUTC Growth

Publications (1)

Publication Number Publication Date
MX2018013469A true MX2018013469A (es) 2020-11-12

Family

ID=49881127

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2015007594A MX364731B (es) 2012-12-14 2013-12-12 Medio enriquecido con nutrientes para el crecimiento de celulas derivadas de tejido de cordon umbilical humano.
MX2018013469A MX2018013469A (es) 2012-12-14 2015-06-12 Medio enriquecido con nutrientes para el crecimiento de celulas derivadas de tejido de cordon umbilical humano.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2015007594A MX364731B (es) 2012-12-14 2013-12-12 Medio enriquecido con nutrientes para el crecimiento de celulas derivadas de tejido de cordon umbilical humano.

Country Status (18)

Country Link
US (1) US20140170748A1 (es)
EP (2) EP2931875B1 (es)
JP (2) JP6404227B2 (es)
KR (2) KR101948237B1 (es)
CN (2) CN105026551B (es)
AR (2) AR093985A1 (es)
AU (2) AU2013359265B2 (es)
BR (1) BR112015013685A8 (es)
CA (1) CA2895079C (es)
ES (1) ES2667573T3 (es)
HK (1) HK1215050A1 (es)
MX (2) MX364731B (es)
PH (1) PH12015501310A1 (es)
PL (1) PL2931875T3 (es)
RU (2) RU2684306C2 (es)
SG (2) SG11201504547SA (es)
TW (1) TWI620818B (es)
WO (1) WO2014093598A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10791730B2 (en) * 2016-01-14 2020-10-06 DePuy Synthes Products, Inc. Composition and methods for cryopreservation of hUTC
US11111480B2 (en) 2016-04-29 2021-09-07 Hope Biosctences, Llc Culture media for multipotent stem cells
US10894947B1 (en) * 2016-04-29 2021-01-19 Hope Biosciences, Llc Method for generating protein rich conditioned medium
CN106434532A (zh) * 2016-12-22 2017-02-22 叶宗耀 一种培养肝细胞的培养基及其制备方法
CN106957815B (zh) * 2017-03-16 2021-05-07 杨涛 一种用于人类多潜能干细胞的无血清培养基的配方
EP3604503A4 (en) * 2017-03-28 2020-12-30 Ajinomoto Co., Inc. ADDITIVE FOR DE-DIFFERENTIATION SUPPORT MEDIUM
CN107119017B (zh) * 2017-05-25 2020-05-26 山东省医药生物技术研究中心 一种骨肉瘤细胞的无血清培养基及其制备方法
JPWO2020067502A1 (ja) * 2018-09-28 2021-08-30 味の素株式会社 動物細胞の培養用添加物、培養用培地および培養方法
JP7474520B2 (ja) * 2019-04-05 2024-04-25 ザ・ユニバーシティ・オブ・ホンコン 哺乳類の拡大ポテンシャル幹細胞のための培地、その組成物および方法
CN114058573B (zh) * 2020-08-03 2024-04-05 上海原天生物科技有限公司 一种含有生物素的培养基
CN112608892B (zh) * 2020-12-25 2023-11-14 深圳博雅感知药业有限公司 血小板裂解物用于无血清分离和传代培养脐带间充质干细胞的方法
KR20220104321A (ko) 2021-01-18 2022-07-26 주식회사 엑소코바이오 엑소좀 및/또는 세포외 소포체의 생성 촉진 내지는 생산성 향상 방법
US11735303B2 (en) 2021-06-22 2023-08-22 David Haase Apparatus and method for determining a composition of a replacement therapy treatment

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA776251B (en) * 1976-11-11 1978-07-26 Massachusetts Inst Technology Improved cell culture microcarriers
CA2074363C (en) * 1990-01-22 2004-11-09 David Thomas Vistica Co2-independent growth medium for maintenance and propagation of cells
JP2993614B2 (ja) * 1990-11-20 1999-12-20 有限会社野々川商事 新規化粧料、グルコサミノグリカン生成促進剤および美白剤
US7556958B1 (en) 1997-04-08 2009-07-07 The Rockefeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
WO2002066603A2 (en) * 2001-02-15 2002-08-29 Centocor, Inc. Chemically defined medium for cultured mammalian cells
US9969980B2 (en) 2001-09-21 2018-05-15 Garnet Biotherapeutics Cell populations which co-express CD49c and CD90
WO2003057865A1 (es) * 2002-01-09 2003-07-17 Derbiotek, S.L. Composicion y procedimiento para crear, regenerar y reparar tejidos mediante implante biologico portador de celulas y enriquecido con concentrado de plaquetas mas suplementos.
US8518390B2 (en) * 2003-06-27 2013-08-27 Advanced Technologies And Regenerative Medicine, Llc Treatment of stroke and other acute neural degenerative disorders via intranasal administration of umbilical cord-derived cells
PL1641914T3 (pl) * 2003-06-27 2017-01-31 DePuy Synthes Products, Inc. Komórki pochodzące z poporodowej tkanki łożyska oraz sposoby uzyskiwania i zastosowania tych komórek
WO2005007863A2 (en) 2003-07-10 2005-01-27 National Corn Growers Association Distillers solubles as the primary constituent in protein blocks for livestock
EP1682075A4 (en) * 2003-11-07 2009-12-09 Univ Florida CULTURE AND DIFFERENTIATION OF NEURONAL PRECURSOR CELLS
EP1812557A4 (en) * 2004-10-29 2009-11-04 Centocor Ortho Biotech Inc COMPOSITIONS OF CHEMICALLY DEFINED MEDIA
ES2621847T3 (es) * 2004-12-23 2017-07-05 DePuy Synthes Products, Inc. Células posparto derivadas de tejido de cordón umbilical, y métodos de elaboración y uso de las mismas
US8741638B2 (en) 2005-12-19 2014-06-03 DePuy Synthes Products, LLC In vitro expansion of postpartum-derived cells in roller bottles
WO2008004990A2 (en) * 2006-07-06 2008-01-10 Es Cell International Pte Ltd Method for stem cell culture and cells derived therefrom
ES2524443T3 (es) * 2006-11-13 2014-12-09 DePuy Synthes Products, LLC Expansión in vitro de células postparto usando microportadores
WO2008082525A1 (en) * 2006-12-19 2008-07-10 National Stem Cell Inc Umbilical cord stem cell secreted product derived topical compositions and methods of use thereof
EP2028268A1 (en) * 2007-08-20 2009-02-25 Université Libre De Bruxelles Generation of neuronal cells from pluripotent stem cells
CN100482783C (zh) * 2007-11-27 2009-04-29 天津百若克医药生物技术有限责任公司 一种用于Vero细胞的培养基及其培养方法
US10179900B2 (en) * 2008-12-19 2019-01-15 DePuy Synthes Products, Inc. Conditioned media and methods of making a conditioned media
WO2011017050A1 (en) * 2009-07-28 2011-02-10 Corning Incorporated Synthetic microcarriers for culturing cells

Also Published As

Publication number Publication date
TW201439321A (zh) 2014-10-16
TWI620818B (zh) 2018-04-11
EP3382007A1 (en) 2018-10-03
SG11201504547SA (en) 2015-07-30
EP2931875B1 (en) 2018-03-21
JP2016501026A (ja) 2016-01-18
AR118040A2 (es) 2021-09-15
KR20190016612A (ko) 2019-02-18
US20140170748A1 (en) 2014-06-19
PL2931875T3 (pl) 2018-08-31
AR093985A1 (es) 2015-07-01
AU2013359265A1 (en) 2015-07-16
RU2015128276A (ru) 2017-01-23
RU2653449C2 (ru) 2018-05-08
RU2018112452A (ru) 2019-03-01
PH12015501310B1 (en) 2015-09-02
AU2013359265B2 (en) 2019-01-31
KR102151588B1 (ko) 2020-09-04
SG10201801924VA (en) 2018-05-30
JP6595061B2 (ja) 2019-10-23
CN105026551B (zh) 2020-07-03
EP2931875A1 (en) 2015-10-21
JP6404227B2 (ja) 2018-10-10
CN111690602A (zh) 2020-09-22
CA2895079A1 (en) 2014-06-19
KR101948237B1 (ko) 2019-02-14
RU2018112452A3 (es) 2019-03-01
BR112015013685A8 (pt) 2019-11-05
WO2014093598A1 (en) 2014-06-19
RU2684306C2 (ru) 2019-04-05
JP2018183195A (ja) 2018-11-22
BR112015013685A2 (pt) 2017-07-11
PH12015501310A1 (en) 2015-09-02
CN105026551A (zh) 2015-11-04
ES2667573T3 (es) 2018-05-11
AU2019202191A1 (en) 2019-04-18
HK1215050A1 (zh) 2016-08-12
AU2019202191B2 (en) 2021-01-21
CA2895079C (en) 2021-06-29
MX364731B (es) 2019-05-06
KR20150094760A (ko) 2015-08-19
MX2015007594A (es) 2016-03-15

Similar Documents

Publication Publication Date Title
MX2018013469A (es) Medio enriquecido con nutrientes para el crecimiento de celulas derivadas de tejido de cordon umbilical humano.
WO2011159359A3 (en) Serum-free chemically defined cell culture medium
AR117697A2 (es) Medios de cultivo celular
NZ707059A (en) Methods of culturing microorganisms in non-axenic mixotrophic conditions
MX2021008627A (es) Medio de cultivo celular libre de suero.
MY161726A (en) Biological methods for preparing adipic acid
MX2019010034A (es) Metodo para cultivar celulas eucarionticas.
SG170021A1 (en) Compositions and methods useful for culturing differentiable cells
IN2014MN01680A (es)
MX349658B (es) Metodo para la diferenciacion de las celulas madre pluripotentes en celulas de lecho vascular.
JP2013545489A5 (es)
MX2016013223A (es) Formulacion en polvo de medio de compuesto y metodo de preparacion de medio liquido para cultivo celular.
PL2752484T3 (pl) Sposób wytwarzania podstawowej pożywki hodowlanej dla mezenchymalnych komórek macierzystych, podstawowa pożywka hodowlana dla mezenchymalnych komórek macierzystych i komórkowy środek terapeutyczny hodowany i różnicowany z jej użyciem
WO2008136398A1 (ja) 高濃度アミノ酸含有培地を用いた細胞の培養方法
EP2582792A4 (en) HEART OF CARDIOMYOCYTES WITH DIALYSATED SERUM
PH12014502870A1 (en) Method of producing recombinant iduronate-2-sulfatase
IN2015DN02630A (es)
MY161151A (en) Method and bioreactor for the cultivation of microorganisms
EA201491785A1 (ru) Способ ферментации
CN104357379A (zh) 干细胞培养基
MX353243B (es) Metodo para incrementar la secrecion de proteinas recombinantes.
Gupta Mixotrophic cultivation of microalgae using glycerol, and its effect on nutrient removal from municipal wastewater
NZ616977A (en) Culture medium for human mesenchymal stem cells
UA101881U (ru) Способ приготовления питательной среды для размножения мезенхимальных стволовых клеток человека и их производных
UA84665U (ru) Способ стабилизации рн питательных сред для культивирования молочнокислых бактерий