BR112015013685A2 - meios enriquecidos de nutrientes para o crescimento de hutc - Google Patents

meios enriquecidos de nutrientes para o crescimento de hutc

Info

Publication number
BR112015013685A2
BR112015013685A2 BR112015013685A BR112015013685A BR112015013685A2 BR 112015013685 A2 BR112015013685 A2 BR 112015013685A2 BR 112015013685 A BR112015013685 A BR 112015013685A BR 112015013685 A BR112015013685 A BR 112015013685A BR 112015013685 A2 BR112015013685 A2 BR 112015013685A2
Authority
BR
Brazil
Prior art keywords
serum
transferrin
ethanolamine
vitamins
insulin
Prior art date
Application number
BR112015013685A
Other languages
English (en)
Other versions
BR112015013685A8 (pt
Inventor
S Klaudyne Hong L
Bhatia Ravinder
S Ozturk Sadettin
H Kamaraju Venkat
Original Assignee
Depuy Synthes Products Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Depuy Synthes Products Inc filed Critical Depuy Synthes Products Inc
Publication of BR112015013685A2 publication Critical patent/BR112015013685A2/pt
Publication of BR112015013685A8 publication Critical patent/BR112015013685A8/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

abstract this invention provides for methods of growing anchorage-dependent cells (e.g. hutc) in culture medium comprising amino acids, vitamins, salts nucleosides, insulin, transferrin, ethanolamine and sodium selenium, wherein the culture medium is supplemented with serum. the method further comprises addition of a serum-free nutrient solution comprising amino acids, vitamins, salts nucleosides, insulin, transferrin, ethanolamine and sodium selenium. the invention also provides for culture media and serum-free nutrient solutions for growing anchorage-dependent cells. tradução do resumo resumo patente de invenção "meios enriquecidos de nutrientes para o crescimento de hutc". esta invenção fornece métodos de crescimento de células dependentes de ancoragem (por exemplo hutc) em meio de cultura que compreende aminoácidos, vitaminas, sais, nucleosídeos, insulina, transferrina, etanolamina e sódio selênio, em que o meio de cultura é suplementado com soro. o método adicionalmente compreende a adição de uma solução de nutrientes isenta de soro que compreende aminoácidos, vitaminas, sais, nucleosídeos, insulina, transferrina, etanolamina e sódio selênio. a invenção também fornece meios de cultura e soluções de nutrientes isentas de soro para o crescimento de células dependentes de ancoragem.
BR112015013685A 2012-12-14 2013-12-12 meio de cultura e solução de nutriente isenta de soro para crescimento de células dependentes de ancoragem e método para cultivar células derivadas de tecido do cordão umbilical isoladas BR112015013685A8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/715,532 US20140170748A1 (en) 2012-12-14 2012-12-14 Nutrient Enriched Media for hUTC Growth
PCT/US2013/074615 WO2014093598A1 (en) 2012-12-14 2013-12-12 NUTRIENT ENRICHED MEDIA FOR hUTC GROWTH

Publications (2)

Publication Number Publication Date
BR112015013685A2 true BR112015013685A2 (pt) 2017-07-11
BR112015013685A8 BR112015013685A8 (pt) 2019-11-05

Family

ID=49881127

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015013685A BR112015013685A8 (pt) 2012-12-14 2013-12-12 meio de cultura e solução de nutriente isenta de soro para crescimento de células dependentes de ancoragem e método para cultivar células derivadas de tecido do cordão umbilical isoladas

Country Status (18)

Country Link
US (1) US20140170748A1 (pt)
EP (2) EP3382007A1 (pt)
JP (2) JP6404227B2 (pt)
KR (2) KR101948237B1 (pt)
CN (2) CN111690602A (pt)
AR (2) AR093985A1 (pt)
AU (2) AU2013359265B2 (pt)
BR (1) BR112015013685A8 (pt)
CA (1) CA2895079C (pt)
ES (1) ES2667573T3 (pt)
HK (1) HK1215050A1 (pt)
MX (2) MX364731B (pt)
PH (1) PH12015501310A1 (pt)
PL (1) PL2931875T3 (pt)
RU (2) RU2684306C2 (pt)
SG (2) SG11201504547SA (pt)
TW (1) TWI620818B (pt)
WO (1) WO2014093598A1 (pt)

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MX2018008712A (es) * 2016-01-14 2019-06-06 Depuy Synthes Products Inc Composicion y metodos para la crioconservacion de hutc.
US10894947B1 (en) * 2016-04-29 2021-01-19 Hope Biosciences, Llc Method for generating protein rich conditioned medium
US11111480B2 (en) 2016-04-29 2021-09-07 Hope Biosctences, Llc Culture media for multipotent stem cells
CN106434532A (zh) * 2016-12-22 2017-02-22 叶宗耀 一种培养肝细胞的培养基及其制备方法
CN106957815B (zh) * 2017-03-16 2021-05-07 杨涛 一种用于人类多潜能干细胞的无血清培养基的配方
CN110494559B (zh) * 2017-03-28 2023-10-31 味之素株式会社 不分化地维持用培养基添加剂
CN107119017B (zh) * 2017-05-25 2020-05-26 山东省医药生物技术研究中心 一种骨肉瘤细胞的无血清培养基及其制备方法
KR20210068488A (ko) * 2018-09-28 2021-06-09 아지노모토 가부시키가이샤 동물 세포의 배양용 첨가물, 배양용 배지 및 배양 방법
CN113966393A (zh) * 2019-04-05 2022-01-21 香港大学 哺乳动物扩增潜能干细胞的培养基、组合物及其方法
CN114058573B (zh) * 2020-08-03 2024-04-05 上海原天生物科技有限公司 一种含有生物素的培养基
CN112608892B (zh) * 2020-12-25 2023-11-14 深圳博雅感知药业有限公司 血小板裂解物用于无血清分离和传代培养脐带间充质干细胞的方法
KR20220104321A (ko) 2021-01-18 2022-07-26 주식회사 엑소코바이오 엑소좀 및/또는 세포외 소포체의 생성 촉진 내지는 생산성 향상 방법
US11735303B2 (en) 2021-06-22 2023-08-22 David Haase Apparatus and method for determining a composition of a replacement therapy treatment

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Also Published As

Publication number Publication date
RU2653449C2 (ru) 2018-05-08
BR112015013685A8 (pt) 2019-11-05
CN105026551A (zh) 2015-11-04
EP2931875B1 (en) 2018-03-21
PH12015501310B1 (en) 2015-09-02
JP2018183195A (ja) 2018-11-22
AU2013359265B2 (en) 2019-01-31
PH12015501310A1 (en) 2015-09-02
JP6404227B2 (ja) 2018-10-10
CA2895079C (en) 2021-06-29
AR118040A2 (es) 2021-09-15
RU2018112452A3 (pt) 2019-03-01
EP2931875A1 (en) 2015-10-21
SG10201801924VA (en) 2018-05-30
KR101948237B1 (ko) 2019-02-14
CN105026551B (zh) 2020-07-03
MX2015007594A (es) 2016-03-15
WO2014093598A1 (en) 2014-06-19
SG11201504547SA (en) 2015-07-30
RU2015128276A (ru) 2017-01-23
AU2019202191B2 (en) 2021-01-21
CA2895079A1 (en) 2014-06-19
KR20150094760A (ko) 2015-08-19
KR102151588B1 (ko) 2020-09-04
AU2019202191A1 (en) 2019-04-18
RU2018112452A (ru) 2019-03-01
RU2684306C2 (ru) 2019-04-05
US20140170748A1 (en) 2014-06-19
TW201439321A (zh) 2014-10-16
AU2013359265A1 (en) 2015-07-16
PL2931875T3 (pl) 2018-08-31
ES2667573T3 (es) 2018-05-11
AR093985A1 (es) 2015-07-01
KR20190016612A (ko) 2019-02-18
CN111690602A (zh) 2020-09-22
EP3382007A1 (en) 2018-10-03
HK1215050A1 (zh) 2016-08-12
MX2018013469A (es) 2020-11-12
JP6595061B2 (ja) 2019-10-23
MX364731B (es) 2019-05-06
JP2016501026A (ja) 2016-01-18
TWI620818B (zh) 2018-04-11

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B11D Dismissal acc. art. 38, par 2 of ipl - failure to pay fee after grant in time