WO2008136398A1 - 高濃度アミノ酸含有培地を用いた細胞の培養方法 - Google Patents

高濃度アミノ酸含有培地を用いた細胞の培養方法 Download PDF

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Publication number
WO2008136398A1
WO2008136398A1 PCT/JP2008/058046 JP2008058046W WO2008136398A1 WO 2008136398 A1 WO2008136398 A1 WO 2008136398A1 JP 2008058046 W JP2008058046 W JP 2008058046W WO 2008136398 A1 WO2008136398 A1 WO 2008136398A1
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amino acid
culture
cell culture
culture method
medium
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PCT/JP2008/058046
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English (en)
French (fr)
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Satoshi Katayama
Shouhei Kishishita
Kunihiko Kodaira
Makoto Sadamitsu
Yoshinori Takagi
Hiroki Matsuda
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Chugai Seiyaku Kabushiki Kaisha
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Application filed by Chugai Seiyaku Kabushiki Kaisha filed Critical Chugai Seiyaku Kabushiki Kaisha
Priority to EP08752120.9A priority Critical patent/EP2154244B1/en
Priority to JP2009512972A priority patent/JP5576115B2/ja
Priority to ES08752120.9T priority patent/ES2624185T3/es
Priority to US12/451,003 priority patent/US20110137012A1/en
Priority to DK08752120.9T priority patent/DK2154244T3/en
Publication of WO2008136398A1 publication Critical patent/WO2008136398A1/ja
Priority to US16/297,086 priority patent/US11661579B2/en
Priority to US18/309,489 priority patent/US20230272336A1/en

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Abstract

　所望のタンパク質を生産する細胞を培養して当該タンパク質を製造するための、生物由来培地成分を極力排除した培地による新規な培養方法を提供する。すなわち、培養液中の特定のアミノ酸を高濃度に維持して培養することを特徴とする培養方法、およびこの方法に用いる細胞培養用流加培地を提供する。
PCT/JP2008/058046 2007-04-26 2008-04-25 高濃度アミノ酸含有培地を用いた細胞の培養方法 WO2008136398A1 (ja)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP08752120.9A EP2154244B1 (en) 2007-04-26 2008-04-25 Cell culture method using amino acid-enriched medium
JP2009512972A JP5576115B2 (ja) 2007-04-26 2008-04-25 高濃度アミノ酸含有培地を用いた細胞の培養方法
ES08752120.9T ES2624185T3 (es) 2007-04-26 2008-04-25 Método de cultivo celular que utiliza un medio enriquecido con aminoácidos
US12/451,003 US20110137012A1 (en) 2007-04-26 2008-04-25 Cell culture method using amino acid-enriched medium
DK08752120.9T DK2154244T3 (en) 2007-04-26 2008-04-25 CELL CULTIVATION PROCEDURE WHEN AN ACID-ENRICHED MEDIUM IS USED
US16/297,086 US11661579B2 (en) 2007-04-26 2019-03-08 Cell culture method using amino acid-enriched medium
US18/309,489 US20230272336A1 (en) 2007-04-26 2023-04-28 Cell culture method using amino acid-enriched medium

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007117426 2007-04-26
JP2007-117426 2007-04-26

Related Child Applications (2)

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US12/451,003 A-371-Of-International US20110137012A1 (en) 2007-04-26 2008-04-25 Cell culture method using amino acid-enriched medium
US16/297,086 Division US11661579B2 (en) 2007-04-26 2019-03-08 Cell culture method using amino acid-enriched medium

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WO2008136398A1 true WO2008136398A1 (ja) 2008-11-13

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PCT/JP2008/058046 WO2008136398A1 (ja) 2007-04-26 2008-04-25 高濃度アミノ酸含有培地を用いた細胞の培養方法

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US (3) US20110137012A1 (ja)
EP (1) EP2154244B1 (ja)
JP (1) JP5576115B2 (ja)
KR (1) KR101574355B1 (ja)
CN (2) CN101663391A (ja)
DK (1) DK2154244T3 (ja)
ES (1) ES2624185T3 (ja)
HK (1) HK1224337A1 (ja)
TW (1) TWI601823B (ja)
WO (1) WO2008136398A1 (ja)

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WO2010050448A1 (ja) * 2008-10-28 2010-05-06 中外製薬株式会社 ペプチド含有動物細胞培養用培地
WO2012017925A1 (ja) 2010-08-02 2012-02-09 協和発酵キリン株式会社 物質の製造方法
JP2013524824A (ja) * 2010-04-26 2013-06-20 ノバルティス アーゲー 改良型細胞培養培地
JP2013536683A (ja) * 2010-08-31 2013-09-26 フリエスランド ブランズ ビー．ブイ． 真核細胞のための培養培地
JP2017523798A (ja) * 2014-08-11 2017-08-24 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 真核細胞の比生産速度を増加させる方法
JP2019500878A (ja) * 2016-01-06 2019-01-17 オンコバイオロジクス，インコーポレイティド モノクローナル抗体組成物中の高分子量種、酸性荷電種、及び断片の低減
US10662237B2 (en) 2009-08-06 2020-05-26 Genentech, Inc. Method to improve virus filtration capacity
WO2021090888A1 (ja) 2019-11-05 2021-05-14 味の素株式会社 タンパク質の製造方法

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DK2154244T3 (en) 2007-04-26 2017-06-12 Chugai Pharmaceutical Co Ltd CELL CULTIVATION PROCEDURE WHEN AN ACID-ENRICHED MEDIUM IS USED
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WO2013040444A1 (en) 2011-09-16 2013-03-21 Amgen Inc. Pre-programmed non-feedback controlled continuous feeding of cell cultures
KR101318498B1 (ko) * 2012-03-08 2013-10-16 한화케미칼 주식회사 재조합 단백질의 생산성 향상을 위한 배양용 배지 첨가용 조성물
US11390663B2 (en) 2013-10-11 2022-07-19 Regeneron Pharmaceuticals, Inc. Metabolically optimized cell culture
CN109563161A (zh) 2016-02-03 2019-04-02 安口生物公司 用于提高抗体稳定性的缓冲制剂
TWI763690B (zh) * 2016-07-22 2022-05-11 日商日產化學工業股份有限公司 液狀之培養基組成物之製造方法及製造裝置
WO2018018613A1 (zh) 2016-07-29 2018-02-01 广东东阳光药业有限公司 一种提高抗体纯度的细胞培养基和培养方法
CN113337562B (zh) * 2021-05-24 2022-06-03 宁波人健药业集团股份有限公司 使用CHO细胞高效发酵生产rhCG的方法
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Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8252557B2 (en) 2008-10-28 2012-08-28 Chugai Seiyaku Kabushiki Kaisha Peptide-containing culture medium for culturing animal cell
WO2010050448A1 (ja) * 2008-10-28 2010-05-06 中外製薬株式会社 ペプチド含有動物細胞培養用培地
US10662237B2 (en) 2009-08-06 2020-05-26 Genentech, Inc. Method to improve virus filtration capacity
US11225513B2 (en) 2009-08-06 2022-01-18 Genentech, Inc. Method to improve virus filtration capacity
JP2013524824A (ja) * 2010-04-26 2013-06-20 ノバルティス アーゲー 改良型細胞培養培地
WO2012017925A1 (ja) 2010-08-02 2012-02-09 協和発酵キリン株式会社 物質の製造方法
JPWO2012017925A1 (ja) * 2010-08-02 2013-10-03 協和発酵キリン株式会社 物質の製造方法
JP2013536683A (ja) * 2010-08-31 2013-09-26 フリエスランド ブランズ ビー．ブイ． 真核細胞のための培養培地
JP2017523798A (ja) * 2014-08-11 2017-08-24 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 真核細胞の比生産速度を増加させる方法
JP2019500878A (ja) * 2016-01-06 2019-01-17 オンコバイオロジクス，インコーポレイティド モノクローナル抗体組成物中の高分子量種、酸性荷電種、及び断片の低減
WO2021090888A1 (ja) 2019-11-05 2021-05-14 味の素株式会社 タンパク質の製造方法
JPWO2021090888A1 (ja) * 2019-11-05 2021-05-14
CN114667351A (zh) * 2019-11-05 2022-06-24 味之素株式会社 蛋白质的制造方法
KR20220097421A (ko) 2019-11-05 2022-07-07 아지노모토 가부시키가이샤 단백질의 제조 방법
JP7513034B2 (ja) 2019-11-05 2024-07-09 味の素株式会社 タンパク質の製造方法

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