AR093985A1 - Medios enriquecidos con nutrientes para el cultivo de hutc - Google Patents
Medios enriquecidos con nutrientes para el cultivo de hutcInfo
- Publication number
- AR093985A1 AR093985A1 ARP130104702A ARP130104702A AR093985A1 AR 093985 A1 AR093985 A1 AR 093985A1 AR P130104702 A ARP130104702 A AR P130104702A AR P130104702 A ARP130104702 A AR P130104702A AR 093985 A1 AR093985 A1 AR 093985A1
- Authority
- AR
- Argentina
- Prior art keywords
- hutc
- culture
- serum
- nutrients
- transferrin
- Prior art date
Links
- 235000015097 nutrients Nutrition 0.000 title abstract 3
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 abstract 4
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 4
- 239000001963 growth medium Substances 0.000 abstract 3
- 102000004877 Insulin Human genes 0.000 abstract 2
- 108090001061 Insulin Proteins 0.000 abstract 2
- 102000004338 Transferrin Human genes 0.000 abstract 2
- 108090000901 Transferrin Proteins 0.000 abstract 2
- 150000001413 amino acids Chemical class 0.000 abstract 2
- 230000001419 dependent effect Effects 0.000 abstract 2
- 229940125396 insulin Drugs 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000002777 nucleoside Substances 0.000 abstract 2
- 125000003835 nucleoside group Chemical group 0.000 abstract 2
- 150000003839 salts Chemical class 0.000 abstract 2
- HVAKSLUOHARFLM-UHFFFAOYSA-N selenium;sodium Chemical compound [Se][Na] HVAKSLUOHARFLM-UHFFFAOYSA-N 0.000 abstract 2
- 239000012581 transferrin Substances 0.000 abstract 2
- 235000013343 vitamin Nutrition 0.000 abstract 2
- 239000011782 vitamin Substances 0.000 abstract 2
- 229940088594 vitamin Drugs 0.000 abstract 2
- 229930003231 vitamin Natural products 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 210000003954 umbilical cord Anatomy 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
- C12N5/0075—General culture methods using substrates using microcarriers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
- C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Hematology (AREA)
- Rheumatology (AREA)
- Pregnancy & Childbirth (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Métodos para cultivar células dependientes del anclaje (por ejemplo, hUTC (células derivadas de tejido de cordón umbilical humano)) en medio de cultivo que comprende aminoácidos, vitaminas, sales, nucleósidos, insulina, transferrina, etanolamina y selenio de sodio, en donde el medio de cultivo se complementa con suero. El método comprende, además, la adición de una solución nutriente libre de suero que comprende aminoácidos, vitaminas, sales, nucleósidos, insulina, transferrina, etanolamina y selenio de sodio. Además, medios de cultivo y soluciones nutrientes libres de suero para cultivar células dependientes del anclaje. Kit.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/715,532 US20140170748A1 (en) | 2012-12-14 | 2012-12-14 | Nutrient Enriched Media for hUTC Growth |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR093985A1 true AR093985A1 (es) | 2015-07-01 |
Family
ID=49881127
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP130104702A AR093985A1 (es) | 2012-12-14 | 2013-12-13 | Medios enriquecidos con nutrientes para el cultivo de hutc |
| ARP200100348A AR118040A2 (es) | 2012-12-14 | 2020-02-10 | Medios enriquecidos con nutrientes para el cultivo de hutc |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP200100348A AR118040A2 (es) | 2012-12-14 | 2020-02-10 | Medios enriquecidos con nutrientes para el cultivo de hutc |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US20140170748A1 (es) |
| EP (2) | EP2931875B1 (es) |
| JP (2) | JP6404227B2 (es) |
| KR (2) | KR101948237B1 (es) |
| CN (2) | CN105026551B (es) |
| AR (2) | AR093985A1 (es) |
| AU (2) | AU2013359265B2 (es) |
| BR (1) | BR112015013685A8 (es) |
| CA (1) | CA2895079C (es) |
| ES (1) | ES2667573T3 (es) |
| HK (1) | HK1215050A1 (es) |
| MX (2) | MX364731B (es) |
| PH (1) | PH12015501310A1 (es) |
| PL (1) | PL2931875T3 (es) |
| RU (2) | RU2684306C2 (es) |
| SG (2) | SG11201504547SA (es) |
| TW (1) | TWI620818B (es) |
| WO (1) | WO2014093598A1 (es) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10791730B2 (en) * | 2016-01-14 | 2020-10-06 | DePuy Synthes Products, Inc. | Composition and methods for cryopreservation of hUTC |
| US11111480B2 (en) | 2016-04-29 | 2021-09-07 | Hope Biosctences, Llc | Culture media for multipotent stem cells |
| US10894947B1 (en) * | 2016-04-29 | 2021-01-19 | Hope Biosciences, Llc | Method for generating protein rich conditioned medium |
| CN106434532A (zh) * | 2016-12-22 | 2017-02-22 | 叶宗耀 | 一种培养肝细胞的培养基及其制备方法 |
| CN106957815B (zh) * | 2017-03-16 | 2021-05-07 | 杨涛 | 一种用于人类多潜能干细胞的无血清培养基的配方 |
| EP3604503A4 (en) * | 2017-03-28 | 2020-12-30 | Ajinomoto Co., Inc. | ADDITIVE FOR DE-DIFFERENTIATION SUPPORT MEDIUM |
| CN107119017B (zh) * | 2017-05-25 | 2020-05-26 | 山东省医药生物技术研究中心 | 一种骨肉瘤细胞的无血清培养基及其制备方法 |
| JPWO2020067502A1 (ja) * | 2018-09-28 | 2021-08-30 | 味の素株式会社 | 動物細胞の培養用添加物、培養用培地および培養方法 |
| JP7474520B2 (ja) * | 2019-04-05 | 2024-04-25 | ザ・ユニバーシティ・オブ・ホンコン | 哺乳類の拡大ポテンシャル幹細胞のための培地、その組成物および方法 |
| CN114058573B (zh) * | 2020-08-03 | 2024-04-05 | 上海原天生物科技有限公司 | 一种含有生物素的培养基 |
| CN112608892B (zh) * | 2020-12-25 | 2023-11-14 | 深圳博雅感知药业有限公司 | 血小板裂解物用于无血清分离和传代培养脐带间充质干细胞的方法 |
| KR20220104321A (ko) | 2021-01-18 | 2022-07-26 | 주식회사 엑소코바이오 | 엑소좀 및/또는 세포외 소포체의 생성 촉진 내지는 생산성 향상 방법 |
| US11735303B2 (en) | 2021-06-22 | 2023-08-22 | David Haase | Apparatus and method for determining a composition of a replacement therapy treatment |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA776251B (en) * | 1976-11-11 | 1978-07-26 | Massachusetts Inst Technology | Improved cell culture microcarriers |
| CA2074363C (en) * | 1990-01-22 | 2004-11-09 | David Thomas Vistica | Co2-independent growth medium for maintenance and propagation of cells |
| JP2993614B2 (ja) * | 1990-11-20 | 1999-12-20 | 有限会社野々川商事 | 新規化粧料、グルコサミノグリカン生成促進剤および美白剤 |
| US7556958B1 (en) | 1997-04-08 | 2009-07-07 | The Rockefeller University | Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof |
| WO2002066603A2 (en) * | 2001-02-15 | 2002-08-29 | Centocor, Inc. | Chemically defined medium for cultured mammalian cells |
| US9969980B2 (en) | 2001-09-21 | 2018-05-15 | Garnet Biotherapeutics | Cell populations which co-express CD49c and CD90 |
| WO2003057865A1 (es) * | 2002-01-09 | 2003-07-17 | Derbiotek, S.L. | Composicion y procedimiento para crear, regenerar y reparar tejidos mediante implante biologico portador de celulas y enriquecido con concentrado de plaquetas mas suplementos. |
| US8518390B2 (en) * | 2003-06-27 | 2013-08-27 | Advanced Technologies And Regenerative Medicine, Llc | Treatment of stroke and other acute neural degenerative disorders via intranasal administration of umbilical cord-derived cells |
| PL1641914T3 (pl) * | 2003-06-27 | 2017-01-31 | DePuy Synthes Products, Inc. | Komórki pochodzące z poporodowej tkanki łożyska oraz sposoby uzyskiwania i zastosowania tych komórek |
| WO2005007863A2 (en) | 2003-07-10 | 2005-01-27 | National Corn Growers Association | Distillers solubles as the primary constituent in protein blocks for livestock |
| EP1682075A4 (en) * | 2003-11-07 | 2009-12-09 | Univ Florida | CULTURE AND DIFFERENTIATION OF NEURONAL PRECURSOR CELLS |
| EP1812557A4 (en) * | 2004-10-29 | 2009-11-04 | Centocor Ortho Biotech Inc | COMPOSITIONS OF CHEMICALLY DEFINED MEDIA |
| ES2621847T3 (es) * | 2004-12-23 | 2017-07-05 | DePuy Synthes Products, Inc. | Células posparto derivadas de tejido de cordón umbilical, y métodos de elaboración y uso de las mismas |
| US8741638B2 (en) | 2005-12-19 | 2014-06-03 | DePuy Synthes Products, LLC | In vitro expansion of postpartum-derived cells in roller bottles |
| WO2008004990A2 (en) * | 2006-07-06 | 2008-01-10 | Es Cell International Pte Ltd | Method for stem cell culture and cells derived therefrom |
| ES2524443T3 (es) * | 2006-11-13 | 2014-12-09 | DePuy Synthes Products, LLC | Expansión in vitro de células postparto usando microportadores |
| WO2008082525A1 (en) * | 2006-12-19 | 2008-07-10 | National Stem Cell Inc | Umbilical cord stem cell secreted product derived topical compositions and methods of use thereof |
| EP2028268A1 (en) * | 2007-08-20 | 2009-02-25 | Université Libre De Bruxelles | Generation of neuronal cells from pluripotent stem cells |
| CN100482783C (zh) * | 2007-11-27 | 2009-04-29 | 天津百若克医药生物技术有限责任公司 | 一种用于Vero细胞的培养基及其培养方法 |
| US10179900B2 (en) * | 2008-12-19 | 2019-01-15 | DePuy Synthes Products, Inc. | Conditioned media and methods of making a conditioned media |
| WO2011017050A1 (en) * | 2009-07-28 | 2011-02-10 | Corning Incorporated | Synthetic microcarriers for culturing cells |
-
2012
- 2012-12-14 US US13/715,532 patent/US20140170748A1/en not_active Abandoned
-
2013
- 2013-12-12 PL PL13812432T patent/PL2931875T3/pl unknown
- 2013-12-12 KR KR1020157018827A patent/KR101948237B1/ko active IP Right Grant
- 2013-12-12 CN CN201380072759.4A patent/CN105026551B/zh not_active Expired - Fee Related
- 2013-12-12 ES ES13812432.6T patent/ES2667573T3/es active Active
- 2013-12-12 CN CN202010511518.7A patent/CN111690602A/zh active Pending
- 2013-12-12 MX MX2015007594A patent/MX364731B/es active IP Right Grant
- 2013-12-12 AU AU2013359265A patent/AU2013359265B2/en not_active Ceased
- 2013-12-12 SG SG11201504547SA patent/SG11201504547SA/en unknown
- 2013-12-12 SG SG10201801924VA patent/SG10201801924VA/en unknown
- 2013-12-12 CA CA2895079A patent/CA2895079C/en active Active
- 2013-12-12 JP JP2015547532A patent/JP6404227B2/ja not_active Expired - Fee Related
- 2013-12-12 EP EP13812432.6A patent/EP2931875B1/en active Active
- 2013-12-12 BR BR112015013685A patent/BR112015013685A8/pt active Search and Examination
- 2013-12-12 WO PCT/US2013/074615 patent/WO2014093598A1/en active Application Filing
- 2013-12-12 EP EP18159408.6A patent/EP3382007A1/en not_active Withdrawn
- 2013-12-12 RU RU2018112452A patent/RU2684306C2/ru active
- 2013-12-12 KR KR1020197003828A patent/KR102151588B1/ko active IP Right Grant
- 2013-12-12 RU RU2015128276A patent/RU2653449C2/ru active
- 2013-12-13 TW TW102146011A patent/TWI620818B/zh not_active IP Right Cessation
- 2013-12-13 AR ARP130104702A patent/AR093985A1/es unknown
-
2015
- 2015-06-09 PH PH12015501310A patent/PH12015501310A1/en unknown
- 2015-06-12 MX MX2018013469A patent/MX2018013469A/es unknown
-
2016
- 2016-03-15 HK HK16102960.4A patent/HK1215050A1/zh not_active IP Right Cessation
-
2018
- 2018-09-04 JP JP2018165126A patent/JP6595061B2/ja not_active Expired - Fee Related
-
2019
- 2019-03-29 AU AU2019202191A patent/AU2019202191B2/en not_active Ceased
-
2020
- 2020-02-10 AR ARP200100348A patent/AR118040A2/es unknown
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |