ES2721664T3 - Formulaciones y proceso para aislar un microorganismo viable de hemocultivos positivos - Google Patents

Formulaciones y proceso para aislar un microorganismo viable de hemocultivos positivos Download PDF

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Publication number
ES2721664T3
ES2721664T3 ES13708630T ES13708630T ES2721664T3 ES 2721664 T3 ES2721664 T3 ES 2721664T3 ES 13708630 T ES13708630 T ES 13708630T ES 13708630 T ES13708630 T ES 13708630T ES 2721664 T3 ES2721664 T3 ES 2721664T3
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Spain
Prior art keywords
buffer
approximately
concentration
positive blood
isolate
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Active
Application number
ES13708630T
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English (en)
Inventor
Susan Kircher
Vanda White
William Brasso
Dyan Luper
James Zhou
Julie Rosales
Jeffery Bruton
John Mantlo
Adrien Malick
Donald Callihan
Ben Turng
Liping Feng
Curtis Gosnell
Shawn Beaty
John Douglass
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Becton Dickinson and Co
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Becton Dickinson and Co
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Publication of ES2721664T3 publication Critical patent/ES2721664T3/es
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/14—Streptococcus; Staphylococcus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Un tampón para aislar y concentrar microorganismos viables de una muestra de hemocultivo positivo que comprende una solución base que comprende un caldo nutritivo; al menos un detergente no iónico que comprende saponina y tritón X-100; al menos un tiol; y opcionalmente, cloruro de amonio, en el que la concentración del caldo nutritivo en el tampón es de aproximadamente 10 g/l a aproximadamente 50 g/l, la concentración de los detergentes no iónicos en el tampón es de aproximadamente 0,01 g/l a aproximadamente 20 g/l, la concentración del al menos un tiol en el tampón es de aproximadamente 0,005 g/l a 4 g/l y la concentración del cloruro de amonio opcional en el tampón, si está presente, es de aproximadamente 0,01 g/l a aproximadamente 80 g/l, en el que las cantidades relativas de la solución base, el al menos un detergente no iónico y el al menos un tiol en el tampón se seleccionan para conservar la viabilidad de S. pneumoniae.
ES13708630T 2012-02-29 2013-02-28 Formulaciones y proceso para aislar un microorganismo viable de hemocultivos positivos Active ES2721664T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261604732P 2012-02-29 2012-02-29
PCT/US2013/028249 WO2013130759A1 (en) 2012-02-29 2013-02-28 Formulations and process for isolating viable microorganism from positive blood cultures

Publications (1)

Publication Number Publication Date
ES2721664T3 true ES2721664T3 (es) 2019-08-02

Family

ID=47844525

Family Applications (1)

Application Number Title Priority Date Filing Date
ES13708630T Active ES2721664T3 (es) 2012-02-29 2013-02-28 Formulaciones y proceso para aislar un microorganismo viable de hemocultivos positivos

Country Status (8)

Country Link
US (2) US10519482B2 (es)
EP (1) EP2820120B1 (es)
JP (1) JP6542531B2 (es)
CN (1) CN104254597A (es)
AU (1) AU2013225974B2 (es)
CA (1) CA2865582C (es)
ES (1) ES2721664T3 (es)
WO (1) WO2013130759A1 (es)

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US10053722B2 (en) 2011-12-21 2018-08-21 Geneohm Sciences Canada Inc. Enrichment and isolation of microbial cells and microbial nucleic acids from a biological sample
EP2979614B1 (en) * 2013-03-29 2016-12-28 FUJIFILM Corporation Trocar
WO2016028684A1 (en) * 2014-08-18 2016-02-25 Becton, Dickinson And Company Method of sample preparation for maldi and automated system therefor
WO2017042819A1 (en) * 2015-09-11 2017-03-16 Molecular Detection Israel Ltd. Methods for isolating microbial cells from a blood sample
KR101692666B1 (ko) 2016-02-03 2017-01-11 가톨릭대학교 산학협력단 혈액 배양 양성 검체에서 균종 동정 및 항균제 감수성 검사를 위한 전처리 방법
CN107236782A (zh) * 2017-06-05 2017-10-10 中国医学科学院北京协和医院 革兰氏阴性菌阳性血培养分离胶及化学试剂前处理方法
GB201801022D0 (en) * 2018-01-22 2018-03-07 Q Linea Ab Single dye concentration determination
CN112368561A (zh) * 2018-05-25 2021-02-12 克维拉公司 用于选择性裂解血细胞和分离微生物细胞的方法和组合物
WO2020223511A1 (en) * 2019-04-30 2020-11-05 SeLux Diagnostics, Inc. Rapid methods for determining microorganism growth in samples of human origin
CN112779176B (zh) * 2019-11-06 2022-07-12 珠海恒屹生物科技有限公司 分离试剂、其制备方法、应用、分离细菌的方法和凝胶提取管
CN111500460B (zh) * 2020-05-12 2022-03-01 山东大学齐鲁医院 一种用于血液中微生物快速纯化和富集的提取液及纯化和富集方法与应用
US20220011298A1 (en) * 2020-07-10 2022-01-13 Acutis Diagnostics, Inc. Methods and compositions for isolation and rapid detection of micro-organisms from blood and bodily fluids
US20240026278A1 (en) 2020-08-20 2024-01-25 Becton, Dickinson And Company Blood cell lysing agent for isolating bacteria from blood culture
CN112345319B (zh) * 2020-09-29 2024-05-17 郑州安图生物工程股份有限公司 一种痰液样本的消化方法
CN112345318B (zh) * 2020-09-29 2024-05-17 郑州安图生物工程股份有限公司 一种复合痰消化液

Family Cites Families (16)

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US5314822A (en) * 1992-10-15 1994-05-24 Merck & Co., Inc. Method of clonal growth of Streptococcus pneumoniae
US5694478A (en) 1994-12-15 1997-12-02 Minnesota Mining And Manufacturing Company Method and apparatus for detecting and identifying microbial colonies
US5702884A (en) * 1996-03-12 1997-12-30 Johnson & Johnson Clinical Diagnostics, Inc. Whole blood sample preparation for polymerase chain reaction using ammonium chloride and a carboxylic acid or metal carboxylate for selective red blood cell lysis
US6245335B1 (en) 1996-05-01 2001-06-12 The Rockefeller University Choline binding proteins for anti-pneumococcal vaccines
US5922593A (en) 1997-05-23 1999-07-13 Becton, Dickinson And Company Microbiological test panel and method therefor
WO1998053301A2 (en) 1997-05-23 1998-11-26 Becton Dickinson And Company Automated microbiological testing apparatus and methods therefor
GB9725839D0 (en) * 1997-12-06 1998-02-04 Baker Matthew J Isolation of nucleic acids
US6849422B1 (en) 2000-05-31 2005-02-01 Becton, Dickinson And Company System and method for analyzing antibiotic susceptibility of biological samples using redox and turbidity measurments to ascertain minimum inhibitory concentrations (MICs)
EP1692511B1 (en) * 2003-12-09 2012-09-19 bioMerieux, Inc. Method for recovering microorganisms from a sample
US7662562B2 (en) * 2004-08-10 2010-02-16 Becton, Dickinson And Company Method for rapid identification of microorganisms
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Also Published As

Publication number Publication date
WO2013130759A1 (en) 2013-09-06
US20200087702A1 (en) 2020-03-19
JP2015508667A (ja) 2015-03-23
JP6542531B2 (ja) 2019-07-10
EP2820120B1 (en) 2019-02-13
AU2013225974A1 (en) 2014-09-18
AU2013225974B2 (en) 2018-08-02
US11225681B2 (en) 2022-01-18
US10519482B2 (en) 2019-12-31
US20150125895A1 (en) 2015-05-07
EP2820120A1 (en) 2015-01-07
CA2865582A1 (en) 2013-09-06
CA2865582C (en) 2021-03-16
CN104254597A (zh) 2014-12-31

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