ES2523998T3 - Método de aislamiento de citoblastos dérmicos - Google Patents

Método de aislamiento de citoblastos dérmicos Download PDF

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Publication number
ES2523998T3
ES2523998T3 ES10817219.8T ES10817219T ES2523998T3 ES 2523998 T3 ES2523998 T3 ES 2523998T3 ES 10817219 T ES10817219 T ES 10817219T ES 2523998 T3 ES2523998 T3 ES 2523998T3
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Spain
Prior art keywords
cytoblast
dermal
isolation method
cells
positive cells
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Active
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ES10817219.8T
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English (en)
Inventor
Tsutomu Soma
Haruyo Yamanishi
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Shiseido Co Ltd
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Shiseido Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Rheumatology (AREA)
  • General Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Un método de aislamiento de citoblastos dérmicos que comprende: someter a células separadas de la piel por tratamiento enzimático a un cultivo en suspensión; y aislar las células positivas de marcadores de citoblastos de las células cultivadas, siendo las células positivas de marcadores de citoblastos las células positivas de CD34.

Description

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Claims (1)

  1. imagen1
ES10817219.8T 2009-09-15 2010-09-15 Método de aislamiento de citoblastos dérmicos Active ES2523998T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2009213291 2009-09-15
JP2009213291 2009-09-15
PCT/JP2010/065981 WO2011034106A1 (ja) 2009-09-15 2010-09-15 真皮幹細胞の単離方法

Publications (1)

Publication Number Publication Date
ES2523998T3 true ES2523998T3 (es) 2014-12-03

Family

ID=43758707

Family Applications (1)

Application Number Title Priority Date Filing Date
ES10817219.8T Active ES2523998T3 (es) 2009-09-15 2010-09-15 Método de aislamiento de citoblastos dérmicos

Country Status (7)

Country Link
US (1) US8962323B2 (es)
EP (1) EP2479258B1 (es)
JP (1) JP5800712B2 (es)
KR (1) KR101717317B1 (es)
CN (1) CN102498205B (es)
ES (1) ES2523998T3 (es)
WO (1) WO2011034106A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103459599B (zh) * 2010-11-23 2017-06-16 库尔纳公司 通过抑制nanog的天然反义转录物而治疗nanog相关疾病
KR101751355B1 (ko) 2011-08-24 2017-07-04 (주)아모레퍼시픽 인간 피부 진피 유래 성체 줄기세포
US20150064141A1 (en) * 2012-04-05 2015-03-05 The Regents Of The University Of California Regenerative sera cells and mesenchymal stem cells
CA2921948C (en) * 2013-09-04 2019-11-19 Otsuka Pharmaceutical Factory, Inc. Method for preparing pluripotent stem cells
KR101700598B1 (ko) * 2014-11-11 2017-01-31 한국원자력의학원 Cd105를 포함하는 줄기세포능을 가진 섬유아세포 분리용 바이오마커 조성물 및 이를 이용한 줄기세포능을 가진 섬유아세포 분리 방법
EP3436035A4 (en) * 2016-03-29 2019-10-23 SmsBiotech, Inc. COMPOSITIONS AND METHODS FOR USE OF SMALL MOBILE STEM CELLS
CN110709506A (zh) 2017-07-18 2020-01-17 株式会社资生堂 间充质干细胞导引剂
EP3656867A1 (en) 2017-07-18 2020-05-27 Shiseido Company, Ltd. Method for screening anti-aging substances
CN110940568B (zh) * 2019-12-17 2022-02-18 武汉友芝友医疗科技股份有限公司 一种用于细胞富集、分离、染色、制片的一体化系统
CN113174365A (zh) * 2021-03-26 2021-07-27 万绵水 一种表皮干细胞的分离及培养方法和细胞悬液
TWI828062B (zh) 2022-02-14 2024-01-01 陞醫生物科技股份有限公司 用於生產外泌體的方法及其所製得的外泌體暨其應用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2004101775A1 (ja) * 2003-05-16 2006-07-13 協和醗酵工業株式会社 新規な成体組織由来の幹細胞およびその用途
CN1253558C (zh) * 2003-09-02 2006-04-26 中国人民解放军第四军医大学口腔医学院 人表皮干细胞的分离与培养方法

Also Published As

Publication number Publication date
JPWO2011034106A1 (ja) 2013-02-14
CN102498205B (zh) 2014-06-11
EP2479258A4 (en) 2013-08-14
CN102498205A (zh) 2012-06-13
KR101717317B1 (ko) 2017-03-16
JP5800712B2 (ja) 2015-10-28
EP2479258B1 (en) 2014-11-12
KR20120068856A (ko) 2012-06-27
US8962323B2 (en) 2015-02-24
US20120208269A1 (en) 2012-08-16
WO2011034106A1 (ja) 2011-03-24
EP2479258A1 (en) 2012-07-25

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