MX348269B - Metodo para producir lamina celular epitelial de pigmento retinal. - Google Patents

Metodo para producir lamina celular epitelial de pigmento retinal.

Info

Publication number
MX348269B
MX348269B MX2013009770A MX2013009770A MX348269B MX 348269 B MX348269 B MX 348269B MX 2013009770 A MX2013009770 A MX 2013009770A MX 2013009770 A MX2013009770 A MX 2013009770A MX 348269 B MX348269 B MX 348269B
Authority
MX
Mexico
Prior art keywords
retinal pigment
pigment epithelial
cell sheet
epithelial cell
epithelial cells
Prior art date
Application number
MX2013009770A
Other languages
English (en)
Other versions
MX2013009770A (es
Inventor
Takahashi Masayo
Okamoto Satoshi
Kamao Hiroyuki
Original Assignee
Riken
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Riken filed Critical Riken
Publication of MX2013009770A publication Critical patent/MX2013009770A/es
Publication of MX348269B publication Critical patent/MX348269B/es

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/14—Eye parts, e.g. lenses, corneal implants; Implanting instruments specially adapted therefor; Artificial eyes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Ophthalmology & Optometry (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Neurology (AREA)
  • Medicinal Chemistry (AREA)
  • Neurosurgery (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Transplantation (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Cardiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)

Abstract

La presente invención se refiere a un método para producir una lámina celular que comprende los siguientes pasos: (a) sembrar y cultivar células epiteliales de pigmento retinal sobre un gel de colágeno fibrótico derivado del tendón del cerdo para formar una lámina celular compuesta por las células epiteliales de pigmento retinal, y (b) degradar el gel de colágeno fibrótico derivado del tendón del cerdo con colagenasa para separar la lámina compuesta por las células epiteliales de pigmento retinal.
MX2013009770A 2011-02-25 2012-02-24 Metodo para producir lamina celular epitelial de pigmento retinal. MX348269B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2011040130 2011-02-25
PCT/JP2012/054631 WO2012115244A1 (ja) 2011-02-25 2012-02-24 網膜色素上皮細胞シートの製造方法

Publications (2)

Publication Number Publication Date
MX2013009770A MX2013009770A (es) 2014-01-23
MX348269B true MX348269B (es) 2017-06-05

Family

ID=46721016

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013009770A MX348269B (es) 2011-02-25 2012-02-24 Metodo para producir lamina celular epitelial de pigmento retinal.

Country Status (14)

Country Link
US (2) US9902933B2 (es)
EP (1) EP2679673B1 (es)
JP (1) JP6011979B2 (es)
KR (1) KR101994492B1 (es)
CN (2) CN107937341A (es)
AU (1) AU2012222255B2 (es)
BR (1) BR112013021514A2 (es)
CA (1) CA2828197C (es)
ES (1) ES2726804T3 (es)
IL (1) IL228087A0 (es)
MX (1) MX348269B (es)
RU (1) RU2628697C2 (es)
SG (2) SG192878A1 (es)
WO (1) WO2012115244A1 (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11033586B2 (en) * 2012-08-24 2021-06-15 Riken Method for producing retinal pigment epithelial cell sheet
EP2980207B1 (en) 2013-03-25 2018-12-05 Foundation for Biomedical Research and Innovation at Kobe Cell sorting method
US20160163505A1 (en) * 2013-07-17 2016-06-09 The Regents Of The University Of California Highly conductive nanocomposite, biological and small molecule materials for enhanced resin conductivity
CN105849254A (zh) * 2013-10-09 2016-08-10 希里欧斯株式会社 用于纯化视网膜色素上皮细胞的方法
CN105829527A (zh) 2013-11-11 2016-08-03 住友化学株式会社 用于制备视网膜色素上皮细胞的方法
SG11201606900PA (en) * 2014-02-21 2016-10-28 Healios Kk Eye disease treatment agent, screening method therefor, and method for predicting rejection response associated with retinal pigment epithelial cell transplant
EP3108891B1 (en) * 2015-02-20 2021-01-06 Sumitomo Dainippon Pharma Co., Ltd. Eye disease treatment agent, screening method therefor, and method for predicting rejection response associated with retinal pigment epithelial cell transplant
JP6799248B2 (ja) * 2016-09-28 2020-12-16 澁谷工業株式会社 細胞シートの切断方法
AU2017372468A1 (en) 2016-12-07 2019-06-13 Mayo Foundation For Medical Education And Research Methods and materials for using fibrin supports for retinal pigment epithelium transplantation
CN108728413A (zh) * 2017-06-13 2018-11-02 中山大学中山眼科中心 一种人多能干细胞来源人视网膜色素上皮细胞的制备和扩增培养方法
CN110106147B (zh) * 2018-04-18 2021-04-13 浙江大学 一种诱导人羊膜上皮细胞向视网膜感光细胞分化的方法及其应用
CN108642014B (zh) * 2018-05-17 2019-10-08 山东兴瑞生物科技有限公司 利用自体免疫细胞制备视网膜色素上皮细胞薄片的方法
WO2020218480A1 (ja) * 2019-04-26 2020-10-29 国立研究開発法人理化学研究所 神経網膜と網膜色素上皮細胞とハイドロゲルとを含む複合体及びその製造方法
CN111714698A (zh) * 2020-05-18 2020-09-29 北京奥泰康医药技术开发有限公司 含有细胞的生物凝胶组合物、其制备方法及应用
WO2021248158A1 (en) * 2020-06-01 2021-12-09 Figene, Llc Fibroblasts as a regenerative cellular source for the treatment of blindness
BR112022025542A2 (pt) 2020-06-19 2023-01-17 Endogena Therapeutics Inc Compostos e seu uso como substâncias terapeuticamente ativas no tratamento e/ou prevenção de doenças envolvendo o epitélio pigmentado da retina
US11541039B2 (en) 2020-10-08 2023-01-03 Endogena Therapeutics, Inc. Compounds and their use as therapeutically active substances in the treatment and/or reducing signs or symptoms of diseases involving the retinal pigment epithelium
WO2023166762A1 (ja) * 2022-03-04 2023-09-07 四国計測工業株式会社 細胞培養基材、細胞培養容器および接着細胞の細胞剥離方法
JP7333573B1 (ja) * 2022-03-04 2023-08-25 四国計測工業株式会社 接着細胞の細胞剥離方法、接着細胞の培養方法および接着細胞培養用のキット
CN117625536B (zh) * 2024-01-26 2024-04-30 中国人民解放军总医院第三医学中心 一种人视网膜色素上皮细胞的纯化、培养方法

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IL94611A (en) 1989-06-05 1994-12-29 Organogenesis Inc Medium for cell cultures containing insulin or growth factor similar to insulin, transferrin or iron ion, triiodothyronine or thyroxine and method of use
AU723614B2 (en) * 1997-02-13 2000-08-31 Bausch & Lomb Surgical, Inc. Method for destroying retinal pigment epithelial cells
WO2005079879A1 (ja) 2004-02-25 2005-09-01 Ihara & Company Ltd. コラーゲンゲルおよびその製造方法
JP2005261292A (ja) 2004-03-18 2005-09-29 Ihara Suisan Kk 細胞シートおよびその製造方法
RU2279886C2 (ru) * 2004-06-10 2006-07-20 Московский научно-исследовательский институт глазных болезней им. Гельмгольца Минздрава РФ Способ улучшения функциональной активности сетчатки при ее патологии различного генеза
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WO2009100253A1 (en) * 2008-02-07 2009-08-13 Ceregene, Inc. Rescue of photoreceptors by intravitreal administation of an expression vector encoding a therapeutic protein
GB0806746D0 (en) * 2008-04-14 2008-05-14 Ucl Business Plc Membrane
ES2750028T3 (es) 2010-05-10 2020-03-24 Tsutomu Yasukawa Método de producción de una lámina celular
WO2012009377A2 (en) * 2010-07-12 2012-01-19 University Of Southern California Biocompatible substrate for facilitating interconnections between stem cells and target tissues and methods for implanting same
US11033586B2 (en) 2012-08-24 2021-06-15 Riken Method for producing retinal pigment epithelial cell sheet

Also Published As

Publication number Publication date
EP2679673A1 (en) 2014-01-01
US9902933B2 (en) 2018-02-27
JP6011979B2 (ja) 2016-10-25
KR101994492B1 (ko) 2019-06-28
SG192878A1 (en) 2013-09-30
WO2012115244A1 (ja) 2012-08-30
CA2828197C (en) 2019-11-12
CA2828197A1 (en) 2012-08-30
AU2012222255A1 (en) 2013-10-10
CN103459593A (zh) 2013-12-18
ES2726804T3 (es) 2019-10-09
IL228087A0 (en) 2013-09-30
MX2013009770A (es) 2014-01-23
AU2012222255B2 (en) 2017-04-13
KR20140033008A (ko) 2014-03-17
CN107937341A (zh) 2018-04-20
RU2013143307A (ru) 2015-03-27
EP2679673B1 (en) 2019-02-20
US20180171292A1 (en) 2018-06-21
RU2628697C2 (ru) 2017-08-21
JPWO2012115244A1 (ja) 2014-07-07
US20140057281A1 (en) 2014-02-27
EP2679673A4 (en) 2014-11-12
SG10201704505TA (en) 2017-06-29
BR112013021514A2 (pt) 2020-09-29

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