MX345399B - Metodo de cultivo de celulas animales. - Google Patents

Metodo de cultivo de celulas animales.

Info

Publication number
MX345399B
MX345399B MX2013007650A MX2013007650A MX345399B MX 345399 B MX345399 B MX 345399B MX 2013007650 A MX2013007650 A MX 2013007650A MX 2013007650 A MX2013007650 A MX 2013007650A MX 345399 B MX345399 B MX 345399B
Authority
MX
Mexico
Prior art keywords
culturing
animal cell
cell culturing
cells
culturing method
Prior art date
Application number
MX2013007650A
Other languages
English (en)
Other versions
MX2013007650A (es
Inventor
Shohei Kishishita
Tomoko Okui
Yasuharu Shinoda
Shinya Takuma
Original Assignee
Chugai Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd
Publication of MX2013007650A publication Critical patent/MX2013007650A/es
Publication of MX345399B publication Critical patent/MX345399B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/303—Liver or Pancreas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a un método para reducir un nivel de picos ácidos de un anticuerpo deseado mientras el anticuerpo se prepara al cultivar una célula animal que produce el anticuerpo, en donde la célula animal es una célula que tiene tal carácter que la productividad del anticuerpo deseado por célula no se eleva o decae a una temperatura más baja que una temperatura de cultivo norma y en donde el cultivo se realiza a la temperatura de cultivo normal por un cierto periodo y después el cultivo se continúa a una temperatura de cultivo reducida a 25-35°C.
MX2013007650A 2010-12-28 2011-12-28 Metodo de cultivo de celulas animales. MX345399B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2010291636 2010-12-28
PCT/JP2011/080478 WO2012091124A1 (ja) 2010-12-28 2011-12-28 動物細胞の培養方法

Publications (2)

Publication Number Publication Date
MX2013007650A MX2013007650A (es) 2013-08-01
MX345399B true MX345399B (es) 2017-01-30

Family

ID=46383211

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013007650A MX345399B (es) 2010-12-28 2011-12-28 Metodo de cultivo de celulas animales.

Country Status (17)

Country Link
US (1) US20130295613A1 (es)
EP (1) EP2660328A4 (es)
JP (1) JP6001456B2 (es)
KR (1) KR101903208B1 (es)
CN (2) CN107629124A (es)
AU (1) AU2011350456B2 (es)
BR (1) BR112013018751B1 (es)
CA (1) CA2822947A1 (es)
HK (1) HK1248242A1 (es)
IL (1) IL227185B (es)
MX (1) MX345399B (es)
MY (1) MY163081A (es)
NZ (1) NZ611862A (es)
RU (1) RU2615448C2 (es)
SG (1) SG191371A1 (es)
WO (1) WO2012091124A1 (es)
ZA (1) ZA201304332B (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
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US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9334319B2 (en) 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
US10144923B2 (en) 2012-12-10 2018-12-04 Seikagaku Corporation Recombinant Factor C and method for producing the same, and method for measuring endotoxin
AR095196A1 (es) * 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
CN103255238A (zh) * 2013-05-15 2013-08-21 江苏泰康生物医药有限公司 一种控制哺乳动物细胞乳酸生成的方法
EP3019528A1 (en) * 2013-07-06 2016-05-18 Cadila Healthcare Limited Improved process for production of monoclonal antibodies
WO2015057910A1 (en) 2013-10-16 2015-04-23 Oncobiologics, Inc. Buffer formulations for enhanced antibody stability
US10696735B2 (en) 2015-01-21 2020-06-30 Outlook Therapeutics, Inc. Modulation of charge variants in a monoclonal antibody composition
CN105777896B (zh) * 2015-03-19 2019-08-16 广东东阳光药业有限公司 一种抗体酸性峰的纯化方法
TWI899515B (zh) 2015-08-04 2025-10-01 美商再生元醫藥公司 補充牛磺酸之細胞培養基及用法
KR101789509B1 (ko) * 2015-11-05 2017-10-26 주식회사 프로젠 재조합 인간 갑상선 자극 호르몬을 포함하는 조성물 및 상기 재조합 인간 갑상선 자극 호르몬의 생산 방법
WO2017120359A1 (en) * 2016-01-06 2017-07-13 Oncobiologics, Inc. Reduction of high molecular weight species, acidic charge species, and fragments in a monoclonal antibody composition
EP3411401A1 (en) 2016-02-03 2018-12-12 Oncobiologics, Inc. Buffer formulations for enhanced antibody stability
US20200377918A1 (en) * 2017-03-31 2020-12-03 Boehringer Ingelheim International Gmbh Perfusion medium
CN111500662A (zh) * 2018-06-25 2020-08-07 深圳市菲鹏生物制药股份有限公司 调节cho-k1表达系统所分泌抗体的酸性峰含量的方法
US12297451B1 (en) 2019-10-25 2025-05-13 Regeneron Pharmaceuticals, Inc. Cell culture medium
KR102682066B1 (ko) * 2021-10-12 2024-07-05 프레스티지바이오로직스 주식회사 항체 집단의 제조 방법
WO2026009803A1 (ja) * 2024-07-05 2026-01-08 国立大学法人九州大学 ヘパリン様物質を製造する方法
CN118471326B (zh) * 2024-07-11 2024-10-01 浙江大学 一种细胞培养过程数据分析方法、系统、设备及介质

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR242986A1 (es) * 1988-09-28 1993-06-30 Lilly Co Eli Un metodo de reduccion de la heterogeneidad de anticuerpos secretados por celulas productoras de anticuerpo.
JP4306813B2 (ja) * 1995-09-19 2009-08-05 アスビオファーマ株式会社 動物細胞の新規培養方法
TW426733B (en) * 1995-10-06 2001-03-21 Merck & Co Inc Method of disrupting cultured cells using an impinging jet device
WO2003083066A2 (en) * 2002-03-27 2003-10-09 Immunex Corporation Methods for increasing polypeptide production
JP4318180B2 (ja) * 2002-07-08 2009-08-19 ジーイー・ヘルスケア・ユーケイ・リミテッド タンパク質の飽和標識用の試薬及び方法
PL1674111T3 (pl) * 2004-07-09 2011-04-29 Chugai Pharmaceutical Co Ltd Przeciwciała anty-glipikan 3
DK3255141T3 (da) * 2006-07-13 2022-01-31 Wyeth Llc Fremstilling af antistoffer med forbedret glycosyleringsmønster
JP5586235B2 (ja) 2007-03-02 2014-09-10 ワイス・エルエルシー ポリペプチドの産生のための細胞培養における銅およびグルタミン酸塩の使用
TW200902708A (en) * 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
JP5868593B2 (ja) * 2007-07-17 2016-02-24 メダレックス・リミテッド・ライアビリティ・カンパニーＭｅｄａｒｅｘ， Ｌ．Ｌ．Ｃ． Ｇｌｙｐｉｃａｎ−３に対するモノクローナル抗体
BRPI0818674B1 (pt) * 2007-10-15 2023-01-17 Chugai Seiyaku Kabushiki Kaisha Métodos para produção de um anticorpo inteiro ou de um fragmento do mesmo e para preparação de fármacos, bem como vetor recombinante
BRPI0821110B8 (pt) * 2007-12-05 2021-05-25 Chugai Pharmaceutical Co Ltd anticorpo de neutralização de anti-nr10/il31ra, composição farmacêutica compreendendo o referido anticorpo e uso do mesmo
ES2560470T3 (es) * 2011-04-29 2016-02-19 Biocon Research Limited Un método para reducir la heterogeneidad de anticuerpos y un proceso de producción de dichos anticuerpos

Also Published As

Publication number Publication date
IL227185B (en) 2018-05-31
MY163081A (en) 2017-08-15
ZA201304332B (en) 2014-08-27
RU2615448C2 (ru) 2017-04-04
NZ611862A (en) 2015-05-29
BR112013018751B1 (pt) 2021-01-05
CA2822947A1 (en) 2012-07-05
HK1248242A1 (zh) 2018-10-12
CN103282509A (zh) 2013-09-04
WO2012091124A1 (ja) 2012-07-05
JP6001456B2 (ja) 2016-10-05
EP2660328A4 (en) 2016-07-13
KR101903208B1 (ko) 2018-10-01
AU2011350456A1 (en) 2013-07-04
JPWO2012091124A1 (ja) 2014-06-05
MX2013007650A (es) 2013-08-01
SG191371A1 (en) 2013-08-30
AU2011350456B2 (en) 2016-05-26
EP2660328A1 (en) 2013-11-06
RU2013135255A (ru) 2015-02-10
KR20130131415A (ko) 2013-12-03
BR112013018751A2 (pt) 2016-08-09
CN107629124A (zh) 2018-01-26
US20130295613A1 (en) 2013-11-07

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