BR112015013685A8 - meio de cultura e solução de nutriente isenta de soro para crescimento de células dependentes de ancoragem e método para cultivar células derivadas de tecido do cordão umbilical isoladas - Google Patents

meio de cultura e solução de nutriente isenta de soro para crescimento de células dependentes de ancoragem e método para cultivar células derivadas de tecido do cordão umbilical isoladas Download PDF

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BR112015013685A8
BR112015013685A8 BR112015013685A BR112015013685A BR112015013685A8 BR 112015013685 A8 BR112015013685 A8 BR 112015013685A8 BR 112015013685 A BR112015013685 A BR 112015013685A BR 112015013685 A BR112015013685 A BR 112015013685A BR 112015013685 A8 BR112015013685 A8 BR 112015013685A8
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serum
anchor
culture medium
cell growth
dependent cell
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BR112015013685A
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BR112015013685A2 (pt
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S Klaudyne Hong L
Bhatia Ravinder
S Ozturk Sadettin
H Kamaraju Venkat
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Depuy Synthes Products Inc
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Publication of BR112015013685A8 publication Critical patent/BR112015013685A8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

"meios enriquecidos de nutrientes para o crescimento de hutc". esta invenção fornece métodos de crescimento de células dependentes de ancoragem (por exemplo hutc) em meio de cultura que compreende aminoácidos, vitaminas, sais, nucleosídeos, insulina, transferrina, etanolamina e sódio selênio, em que o meio de cultura é suplementado com soro. o método adicionalmente compreende a adição de uma solução de nutrientes isenta de soro que compreende aminoácidos, vitaminas, sais, nucleosídeos, insulina, transferrina, etanolamina e sódio selênio. a invenção também fornece meios de cultura e soluções de nutrientes isentas de soro para o crescimento de células dependentes de ancoragem.
BR112015013685A 2012-12-14 2013-12-12 meio de cultura e solução de nutriente isenta de soro para crescimento de células dependentes de ancoragem e método para cultivar células derivadas de tecido do cordão umbilical isoladas BR112015013685A8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/715,532 US20140170748A1 (en) 2012-12-14 2012-12-14 Nutrient Enriched Media for hUTC Growth
PCT/US2013/074615 WO2014093598A1 (en) 2012-12-14 2013-12-12 NUTRIENT ENRICHED MEDIA FOR hUTC GROWTH

Publications (2)

Publication Number Publication Date
BR112015013685A2 BR112015013685A2 (pt) 2017-07-11
BR112015013685A8 true BR112015013685A8 (pt) 2019-11-05

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015013685A BR112015013685A8 (pt) 2012-12-14 2013-12-12 meio de cultura e solução de nutriente isenta de soro para crescimento de células dependentes de ancoragem e método para cultivar células derivadas de tecido do cordão umbilical isoladas

Country Status (18)

Country Link
US (1) US20140170748A1 (pt)
EP (2) EP2931875B1 (pt)
JP (2) JP6404227B2 (pt)
KR (2) KR102151588B1 (pt)
CN (2) CN111690602A (pt)
AR (2) AR093985A1 (pt)
AU (2) AU2013359265B2 (pt)
BR (1) BR112015013685A8 (pt)
CA (1) CA2895079C (pt)
ES (1) ES2667573T3 (pt)
HK (1) HK1215050A1 (pt)
MX (2) MX364731B (pt)
PH (1) PH12015501310A1 (pt)
PL (1) PL2931875T3 (pt)
RU (2) RU2684306C2 (pt)
SG (2) SG10201801924VA (pt)
TW (1) TWI620818B (pt)
WO (1) WO2014093598A1 (pt)

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EP3402329A1 (en) 2016-01-14 2018-11-21 DePuy Synthes Products, Inc. Composition and methods for cryopreservation of hutc
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US11111480B2 (en) 2016-04-29 2021-09-07 Hope Biosctences, Llc Culture media for multipotent stem cells
CN106434532A (zh) * 2016-12-22 2017-02-22 叶宗耀 一种培养肝细胞的培养基及其制备方法
CN106957815B (zh) * 2017-03-16 2021-05-07 杨涛 一种用于人类多潜能干细胞的无血清培养基的配方
KR102641031B1 (ko) * 2017-03-28 2024-02-28 아지노모토 가부시키가이샤 미분화 유지 배지 첨가제
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CN112608892B (zh) * 2020-12-25 2023-11-14 深圳博雅感知药业有限公司 血小板裂解物用于无血清分离和传代培养脐带间充质干细胞的方法
KR20220104321A (ko) 2021-01-18 2022-07-26 주식회사 엑소코바이오 엑소좀 및/또는 세포외 소포체의 생성 촉진 내지는 생산성 향상 방법
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Also Published As

Publication number Publication date
AR118040A2 (es) 2021-09-15
BR112015013685A2 (pt) 2017-07-11
RU2018112452A3 (pt) 2019-03-01
KR102151588B1 (ko) 2020-09-04
MX2015007594A (es) 2016-03-15
RU2015128276A (ru) 2017-01-23
KR20150094760A (ko) 2015-08-19
AR093985A1 (es) 2015-07-01
SG10201801924VA (en) 2018-05-30
AU2013359265A1 (en) 2015-07-16
RU2684306C2 (ru) 2019-04-05
MX2018013469A (es) 2020-11-12
MX364731B (es) 2019-05-06
HK1215050A1 (zh) 2016-08-12
US20140170748A1 (en) 2014-06-19
JP2018183195A (ja) 2018-11-22
KR20190016612A (ko) 2019-02-18
CN105026551A (zh) 2015-11-04
CN105026551B (zh) 2020-07-03
KR101948237B1 (ko) 2019-02-14
PH12015501310B1 (en) 2015-09-02
CN111690602A (zh) 2020-09-22
AU2019202191B2 (en) 2021-01-21
JP6404227B2 (ja) 2018-10-10
AU2013359265B2 (en) 2019-01-31
EP2931875A1 (en) 2015-10-21
ES2667573T3 (es) 2018-05-11
PL2931875T3 (pl) 2018-08-31
PH12015501310A1 (en) 2015-09-02
CA2895079C (en) 2021-06-29
EP3382007A1 (en) 2018-10-03
EP2931875B1 (en) 2018-03-21
TW201439321A (zh) 2014-10-16
AU2019202191A1 (en) 2019-04-18
CA2895079A1 (en) 2014-06-19
JP2016501026A (ja) 2016-01-18
JP6595061B2 (ja) 2019-10-23
WO2014093598A1 (en) 2014-06-19
RU2018112452A (ru) 2019-03-01
SG11201504547SA (en) 2015-07-30
TWI620818B (zh) 2018-04-11
RU2653449C2 (ru) 2018-05-08

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
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