MX364731B - Medio enriquecido con nutrientes para el crecimiento de celulas derivadas de tejido de cordon umbilical humano. - Google Patents

Medio enriquecido con nutrientes para el crecimiento de celulas derivadas de tejido de cordon umbilical humano.

Info

Publication number
MX364731B
MX364731B MX2015007594A MX2015007594A MX364731B MX 364731 B MX364731 B MX 364731B MX 2015007594 A MX2015007594 A MX 2015007594A MX 2015007594 A MX2015007594 A MX 2015007594A MX 364731 B MX364731 B MX 364731B
Authority
MX
Mexico
Prior art keywords
serum
enriched media
hutc
nutrient enriched
transferrin
Prior art date
Application number
MX2015007594A
Other languages
English (en)
Other versions
MX2015007594A (es
Inventor
Ravinder Bhatia
L S Klaudyne Hong
Sadettin S Ozturk
Venkat H Kamaraju
Original Assignee
Depuy Synthes Products Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Depuy Synthes Products Inc filed Critical Depuy Synthes Products Inc
Publication of MX2015007594A publication Critical patent/MX2015007594A/es
Publication of MX364731B publication Critical patent/MX364731B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente invención se refiere a métodos de cultivar células dependientes de anclaje (por ejemplo, hUTC) en medio de cultivo que comprende aminoácidos, vitaminas, sales, nucleósidos, insulina, transferrina, etanolamina y sodio selenio, en donde el medio de cultivo se complementa con suero; el método comprende, además, la adición de una solución nutriente libre de suero que comprende aminoácidos, vitaminas, sales, nucleósidos, insulina, transferrina, etanolamina y sodio selenio; la invención proporciona, además, un medio de cultivo y soluciones nutrientes libre de suero para el cultivo de células dependientes de anclaje.
MX2015007594A 2012-12-14 2013-12-12 Medio enriquecido con nutrientes para el crecimiento de celulas derivadas de tejido de cordon umbilical humano. MX364731B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/715,532 US20140170748A1 (en) 2012-12-14 2012-12-14 Nutrient Enriched Media for hUTC Growth
PCT/US2013/074615 WO2014093598A1 (en) 2012-12-14 2013-12-12 NUTRIENT ENRICHED MEDIA FOR hUTC GROWTH

Publications (2)

Publication Number Publication Date
MX2015007594A MX2015007594A (es) 2016-03-15
MX364731B true MX364731B (es) 2019-05-06

Family

ID=49881127

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2015007594A MX364731B (es) 2012-12-14 2013-12-12 Medio enriquecido con nutrientes para el crecimiento de celulas derivadas de tejido de cordon umbilical humano.
MX2018013469A MX2018013469A (es) 2012-12-14 2015-06-12 Medio enriquecido con nutrientes para el crecimiento de celulas derivadas de tejido de cordon umbilical humano.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2018013469A MX2018013469A (es) 2012-12-14 2015-06-12 Medio enriquecido con nutrientes para el crecimiento de celulas derivadas de tejido de cordon umbilical humano.

Country Status (18)

Country Link
US (1) US20140170748A1 (es)
EP (2) EP2931875B1 (es)
JP (2) JP6404227B2 (es)
KR (2) KR101948237B1 (es)
CN (2) CN105026551B (es)
AR (2) AR093985A1 (es)
AU (2) AU2013359265B2 (es)
BR (1) BR112015013685A8 (es)
CA (1) CA2895079C (es)
ES (1) ES2667573T3 (es)
HK (1) HK1215050A1 (es)
MX (2) MX364731B (es)
PH (1) PH12015501310A1 (es)
PL (1) PL2931875T3 (es)
RU (2) RU2684306C2 (es)
SG (2) SG11201504547SA (es)
TW (1) TWI620818B (es)
WO (1) WO2014093598A1 (es)

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US10791730B2 (en) * 2016-01-14 2020-10-06 DePuy Synthes Products, Inc. Composition and methods for cryopreservation of hUTC
US11111480B2 (en) 2016-04-29 2021-09-07 Hope Biosctences, Llc Culture media for multipotent stem cells
US10894947B1 (en) * 2016-04-29 2021-01-19 Hope Biosciences, Llc Method for generating protein rich conditioned medium
CN106434532A (zh) * 2016-12-22 2017-02-22 叶宗耀 一种培养肝细胞的培养基及其制备方法
CN106957815B (zh) * 2017-03-16 2021-05-07 杨涛 一种用于人类多潜能干细胞的无血清培养基的配方
EP3604503A4 (en) * 2017-03-28 2020-12-30 Ajinomoto Co., Inc. ADDITIVE FOR DE-DIFFERENTIATION SUPPORT MEDIUM
CN107119017B (zh) * 2017-05-25 2020-05-26 山东省医药生物技术研究中心 一种骨肉瘤细胞的无血清培养基及其制备方法
JPWO2020067502A1 (ja) * 2018-09-28 2021-08-30 味の素株式会社 動物細胞の培養用添加物、培養用培地および培養方法
JP7474520B2 (ja) * 2019-04-05 2024-04-25 ザ・ユニバーシティ・オブ・ホンコン 哺乳類の拡大ポテンシャル幹細胞のための培地、その組成物および方法
CN114058573B (zh) * 2020-08-03 2024-04-05 上海原天生物科技有限公司 一种含有生物素的培养基
CN112608892B (zh) * 2020-12-25 2023-11-14 深圳博雅感知药业有限公司 血小板裂解物用于无血清分离和传代培养脐带间充质干细胞的方法
KR20220104321A (ko) 2021-01-18 2022-07-26 주식회사 엑소코바이오 엑소좀 및/또는 세포외 소포체의 생성 촉진 내지는 생산성 향상 방법
US11735303B2 (en) 2021-06-22 2023-08-22 David Haase Apparatus and method for determining a composition of a replacement therapy treatment

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JP2993614B2 (ja) * 1990-11-20 1999-12-20 有限会社野々川商事 新規化粧料、グルコサミノグリカン生成促進剤および美白剤
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WO2003057865A1 (es) * 2002-01-09 2003-07-17 Derbiotek, S.L. Composicion y procedimiento para crear, regenerar y reparar tejidos mediante implante biologico portador de celulas y enriquecido con concentrado de plaquetas mas suplementos.
US8518390B2 (en) * 2003-06-27 2013-08-27 Advanced Technologies And Regenerative Medicine, Llc Treatment of stroke and other acute neural degenerative disorders via intranasal administration of umbilical cord-derived cells
PL1641914T3 (pl) * 2003-06-27 2017-01-31 DePuy Synthes Products, Inc. Komórki pochodzące z poporodowej tkanki łożyska oraz sposoby uzyskiwania i zastosowania tych komórek
WO2005007863A2 (en) 2003-07-10 2005-01-27 National Corn Growers Association Distillers solubles as the primary constituent in protein blocks for livestock
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Also Published As

Publication number Publication date
TW201439321A (zh) 2014-10-16
TWI620818B (zh) 2018-04-11
EP3382007A1 (en) 2018-10-03
SG11201504547SA (en) 2015-07-30
EP2931875B1 (en) 2018-03-21
JP2016501026A (ja) 2016-01-18
AR118040A2 (es) 2021-09-15
KR20190016612A (ko) 2019-02-18
US20140170748A1 (en) 2014-06-19
PL2931875T3 (pl) 2018-08-31
AR093985A1 (es) 2015-07-01
AU2013359265A1 (en) 2015-07-16
RU2015128276A (ru) 2017-01-23
RU2653449C2 (ru) 2018-05-08
RU2018112452A (ru) 2019-03-01
PH12015501310B1 (en) 2015-09-02
AU2013359265B2 (en) 2019-01-31
KR102151588B1 (ko) 2020-09-04
SG10201801924VA (en) 2018-05-30
JP6595061B2 (ja) 2019-10-23
CN105026551B (zh) 2020-07-03
EP2931875A1 (en) 2015-10-21
JP6404227B2 (ja) 2018-10-10
CN111690602A (zh) 2020-09-22
CA2895079A1 (en) 2014-06-19
KR101948237B1 (ko) 2019-02-14
MX2018013469A (es) 2020-11-12
RU2018112452A3 (es) 2019-03-01
BR112015013685A8 (pt) 2019-11-05
WO2014093598A1 (en) 2014-06-19
RU2684306C2 (ru) 2019-04-05
JP2018183195A (ja) 2018-11-22
BR112015013685A2 (pt) 2017-07-11
PH12015501310A1 (en) 2015-09-02
CN105026551A (zh) 2015-11-04
ES2667573T3 (es) 2018-05-11
AU2019202191A1 (en) 2019-04-18
HK1215050A1 (zh) 2016-08-12
AU2019202191B2 (en) 2021-01-21
CA2895079C (en) 2021-06-29
KR20150094760A (ko) 2015-08-19
MX2015007594A (es) 2016-03-15

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