MX349658B - Metodo para la diferenciacion de las celulas madre pluripotentes en celulas de lecho vascular. - Google Patents

Metodo para la diferenciacion de las celulas madre pluripotentes en celulas de lecho vascular.

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Publication number
MX349658B
MX349658B MX2013013854A MX2013013854A MX349658B MX 349658 B MX349658 B MX 349658B MX 2013013854 A MX2013013854 A MX 2013013854A MX 2013013854 A MX2013013854 A MX 2013013854A MX 349658 B MX349658 B MX 349658B
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MX
Mexico
Prior art keywords
cells
stem cells
pluripotent stem
vascular bed
differentiation
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Application number
MX2013013854A
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English (en)
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MX2013013854A (es
Inventor
Christensen Klaus
Graf Martin
Iacone Roberto
Patsch Christoph
Christina Thoma Eva
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Hoffmann La Roche
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Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2013013854A publication Critical patent/MX2013013854A/es
Publication of MX349658B publication Critical patent/MX349658B/es

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/0602—Vertebrate cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C12N5/0691—Vascular smooth muscle cells; 3D culture thereof, e.g. models of blood vessels
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/165—Vascular endothelial growth factor [VEGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Biomedical Technology (AREA)
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  • Biotechnology (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Vascular Medicine (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Tropical Medicine & Parasitology (AREA)
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  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
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  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)

Abstract

La presente invención se refiere a un método para la diferenciación de células madre pluripotentes (PSCs) en células de lecho vascular. Además, esta solicitud se refiere a un método para la diferenciación de células madre embrionarias humanas (ESCs) y células madre pluripotentes inducidas (iPSCs) en células de lecho vascular con base en pasos ligados de inducciones de medio químicamente definido.
MX2013013854A 2011-06-09 2012-06-04 Metodo para la diferenciacion de las celulas madre pluripotentes en celulas de lecho vascular. MX349658B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11169316 2011-06-09
PCT/EP2012/060461 WO2012168167A1 (en) 2011-06-09 2012-06-04 Method for differentiation of pluripotent stem cells into vascular bed cells

Publications (2)

Publication Number Publication Date
MX2013013854A MX2013013854A (es) 2014-01-20
MX349658B true MX349658B (es) 2017-08-08

Family

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Family Applications (1)

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MX2013013854A MX349658B (es) 2011-06-09 2012-06-04 Metodo para la diferenciacion de las celulas madre pluripotentes en celulas de lecho vascular.

Country Status (10)

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US (2) US20150017674A1 (es)
EP (1) EP2718425B1 (es)
JP (1) JP6124880B2 (es)
KR (1) KR20140031300A (es)
CN (1) CN103620024B (es)
BR (1) BR112013030166A2 (es)
CA (1) CA2836843A1 (es)
MX (1) MX349658B (es)
RU (1) RU2618871C2 (es)
WO (1) WO2012168167A1 (es)

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KR101842957B1 (ko) * 2015-11-11 2018-03-28 한국원자력의학원 p38 저해제를 처리하여 단층세포배양을 통한 역분화줄기세포에서 중간엽줄기세포로의 분화방법
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CN112567022A (zh) * 2018-08-21 2021-03-26 豪夫迈·罗氏有限公司 用于评定跨内皮屏障完整性的方法
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JP2022549245A (ja) * 2019-09-20 2022-11-24 エモリー ユニバーシティー 尿の細胞から作製される内皮および平滑筋様組織およびその使用
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Publication number Publication date
CN103620024A (zh) 2014-03-05
RU2618871C2 (ru) 2017-05-11
EP2718425B1 (en) 2017-05-10
CA2836843A1 (en) 2012-12-13
US20200182861A1 (en) 2020-06-11
RU2013156940A (ru) 2015-07-20
WO2012168167A1 (en) 2012-12-13
MX2013013854A (es) 2014-01-20
US20150017674A1 (en) 2015-01-15
BR112013030166A2 (pt) 2017-03-21
JP6124880B2 (ja) 2017-05-10
CN103620024B (zh) 2016-06-22
EP2718425A1 (en) 2014-04-16
JP2014516557A (ja) 2014-07-17
KR20140031300A (ko) 2014-03-12

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