BR112013030166A2 - método de diferenciação de células haste pluripotentes em células leito vasculares, células endoteliais ou células dos músculos moles vasculares, banco biológico e uso de células endoteliais ou células dos músculos moles vasculares, composição terapêutica, métodos e usos. - Google Patents

método de diferenciação de células haste pluripotentes em células leito vasculares, células endoteliais ou células dos músculos moles vasculares, banco biológico e uso de células endoteliais ou células dos músculos moles vasculares, composição terapêutica, métodos e usos.

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Publication number
BR112013030166A2
BR112013030166A2 BR112013030166A BR112013030166A BR112013030166A2 BR 112013030166 A2 BR112013030166 A2 BR 112013030166A2 BR 112013030166 A BR112013030166 A BR 112013030166A BR 112013030166 A BR112013030166 A BR 112013030166A BR 112013030166 A2 BR112013030166 A2 BR 112013030166A2
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BR
Brazil
Prior art keywords
cells
vascular
soft muscle
endothelial
endothelial cells
Prior art date
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BR112013030166A
Other languages
English (en)
Inventor
Patsch Christoph
Christina Thoma Eva
Christensen Klaus
Graf Martin
Iacone Roberto
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of BR112013030166A2 publication Critical patent/BR112013030166A2/pt

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C12N5/0691—Vascular smooth muscle cells; 3D culture thereof, e.g. models of blood vessels
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/165—Vascular endothelial growth factor [VEGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Vascular Medicine (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
BR112013030166A 2011-06-09 2012-06-04 método de diferenciação de células haste pluripotentes em células leito vasculares, células endoteliais ou células dos músculos moles vasculares, banco biológico e uso de células endoteliais ou células dos músculos moles vasculares, composição terapêutica, métodos e usos. BR112013030166A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11169316 2011-06-09
PCT/EP2012/060461 WO2012168167A1 (en) 2011-06-09 2012-06-04 Method for differentiation of pluripotent stem cells into vascular bed cells

Publications (1)

Publication Number Publication Date
BR112013030166A2 true BR112013030166A2 (pt) 2017-03-21

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013030166A BR112013030166A2 (pt) 2011-06-09 2012-06-04 método de diferenciação de células haste pluripotentes em células leito vasculares, células endoteliais ou células dos músculos moles vasculares, banco biológico e uso de células endoteliais ou células dos músculos moles vasculares, composição terapêutica, métodos e usos.

Country Status (10)

Country Link
US (2) US20150017674A1 (pt)
EP (1) EP2718425B1 (pt)
JP (1) JP6124880B2 (pt)
KR (1) KR20140031300A (pt)
CN (1) CN103620024B (pt)
BR (1) BR112013030166A2 (pt)
CA (1) CA2836843A1 (pt)
MX (1) MX349658B (pt)
RU (1) RU2618871C2 (pt)
WO (1) WO2012168167A1 (pt)

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WO2014121449A1 (en) * 2013-02-05 2014-08-14 Guangzhou Institutes Of Biomedicine And Health, Chinese Academy Of Sciences Preparing tooth-like structure using stem cell
US9506037B2 (en) 2013-03-15 2016-11-29 The Johns Hopkins University Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix
WO2014145871A1 (en) * 2013-03-15 2014-09-18 The Johns Hopkins University Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix
US9994825B2 (en) 2013-03-15 2018-06-12 The Johns Hopkins University Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix
WO2014200340A1 (en) * 2013-06-10 2014-12-18 Academisch Ziekenhuis Leiden Differentiation and expansion of endothelial cells from pluripotent stem cells and the in vitro formation of vasculature like structures
EP3027737B1 (en) * 2013-07-29 2018-12-26 F.Hoffmann-La Roche Ag Method for differentiation of pluripotent stem cells into multi-competent renal precursors
EP3039126B1 (en) * 2013-08-26 2019-10-09 The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone Small molecule cellular reprogramming to generate neuronal cells
WO2015058117A1 (en) * 2013-10-18 2015-04-23 Icahn School Of Medicine At Mount Sinai Directed cardiomyocyte differentiation and ventricular specification of stem cells
WO2015169762A1 (en) * 2014-05-06 2015-11-12 F. Hoffmann-La Roche Ag Method for differentiation of pluripotent stem cells into cardiomyocytes
SG10201903910RA (en) * 2014-11-07 2019-05-30 Univ Osaka Differentiation-induced cell population from which undifferentiated cells have been removed, use of same, and method for producing same
US20160168538A1 (en) * 2014-12-15 2016-06-16 The Board Of Trustees Of The University Of Illinois Flk1+ and VE-Cadherin+ Endothelial Cells Derived from iPS or ES Cells, and Methods of Preparing and Using the Same
AU2014277667B2 (en) * 2014-12-15 2022-07-14 The University Of Queensland Differentiation of pluripotent stem cells to form renal organoids
KR101642637B1 (ko) 2015-01-08 2016-07-25 이화여자대학교 산학협력단 편도 유래 중간엽 줄기세포로부터 근육 세포의 분화방법
KR102306231B1 (ko) 2015-06-09 2021-09-28 이화여자대학교 산학협력단 편도 유래 중간엽 줄기세포로부터 건 세포의 분화방법
GB201510913D0 (en) * 2015-06-22 2015-08-05 Nat University Of Singapore And Agency For Science Technology And Res Vascularized tissue, skin or mucosa quivalent
US20180201899A1 (en) * 2015-07-15 2018-07-19 Northwestern University Ipsc-ec performance enhancement via sirt1 overexpression
EP3964565A1 (en) 2015-09-01 2022-03-09 Ncardia B.V. An in vitro method of differentiating a human pluripotent stem cell population into a cardiomyocyte cell population
EP3347451B1 (en) * 2015-09-11 2019-08-28 The General Hospital Corporation Regeneration of a functional pulmonary vascular bed
KR101781693B1 (ko) 2015-09-24 2017-09-26 서울대학교병원 심장내막 유래 성체줄기세포로부터 제조된 유도만능 줄기세포의 심혈관계 세포로의 분화방법 및 이의 용도
KR101842957B1 (ko) * 2015-11-11 2018-03-28 한국원자력의학원 p38 저해제를 처리하여 단층세포배양을 통한 역분화줄기세포에서 중간엽줄기세포로의 분화방법
WO2017136479A1 (en) 2016-02-01 2017-08-10 Cedars-Sinai Medical Center Systems and methods for growth of intestinal cells in microfluidic devices
KR101717402B1 (ko) 2016-07-18 2017-03-16 이화여자대학교 산학협력단 편도 유래 중간엽 줄기세포로부터 근육 세포의 분화방법
WO2018019780A1 (en) * 2016-07-28 2018-02-01 F. Hoffmann-La Roche Ag Non-human primate induced pluripotent stem cell derived hepatocytes and uses thereof
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US11767513B2 (en) 2017-03-14 2023-09-26 Cedars-Sinai Medical Center Neuromuscular junction
WO2018176001A2 (en) 2017-03-24 2018-09-27 Cedars-Sinai Medical Center Methods and compositions for production of fallopian tube epithelium
CN111065731B (zh) * 2017-06-16 2024-03-12 Imba-莫利库尔生物技术研究所 血管类器官、产生和使用所述类器官的方法
CN108384746B (zh) * 2018-02-08 2020-04-21 北京呈诺医学科技有限公司 一种诱导性多能干细胞向成熟内皮细胞高效分化的方法
US20210130774A1 (en) * 2018-04-06 2021-05-06 Cedars-Sinai Medical Center Human pluripotent stem cell derived neurodegenerative disease models on a microfluidic chip
TW202122573A (zh) * 2019-08-28 2021-06-16 安斯泰來再生醫藥協會 治療血管疾病之組成物及方法
KR20220065805A (ko) * 2019-09-20 2022-05-20 에모리 유니버시티 요세포로부터 생성된 조직과 같은 내피 및 평활근 및 이와 관련된 용도
CN114746543A (zh) * 2019-11-13 2022-07-12 延世大学校产学协力团 分离纯培养血管内皮细胞的方法、维持血管内皮细胞特性的培养基和包括其的培养方法
AU2021251255A1 (en) * 2020-04-09 2022-10-27 Emory University Methods of generating pluripotent stem cell-derived vascular smooth muscle cells, uses, and composition related thereto
WO2023107553A1 (en) * 2021-12-08 2023-06-15 United Therapeutics Corporation Methods and compositions for improving endothelial cell barrier
CN114231481B (zh) * 2021-12-21 2023-07-18 中国人民解放军总医院 一种重编程真皮成纤维细胞为内皮祖细胞的化学诱导方法
WO2023230382A1 (en) * 2022-05-27 2023-11-30 President And Fellows Of Harvard College Brain endothelial cells and methods of making

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Publication number Publication date
EP2718425B1 (en) 2017-05-10
CN103620024A (zh) 2014-03-05
RU2618871C2 (ru) 2017-05-11
MX349658B (es) 2017-08-08
WO2012168167A1 (en) 2012-12-13
KR20140031300A (ko) 2014-03-12
RU2013156940A (ru) 2015-07-20
US20200182861A1 (en) 2020-06-11
MX2013013854A (es) 2014-01-20
JP2014516557A (ja) 2014-07-17
US20150017674A1 (en) 2015-01-15
CN103620024B (zh) 2016-06-22
EP2718425A1 (en) 2014-04-16
CA2836843A1 (en) 2012-12-13
JP6124880B2 (ja) 2017-05-10

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