MX353243B - Metodo para incrementar la secrecion de proteinas recombinantes. - Google Patents
Metodo para incrementar la secrecion de proteinas recombinantes.Info
- Publication number
- MX353243B MX353243B MX2014012407A MX2014012407A MX353243B MX 353243 B MX353243 B MX 353243B MX 2014012407 A MX2014012407 A MX 2014012407A MX 2014012407 A MX2014012407 A MX 2014012407A MX 353243 B MX353243 B MX 353243B
- Authority
- MX
- Mexico
- Prior art keywords
- secretion
- increasing
- recombinant proteins
- cells
- cell wall
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 230000028327 secretion Effects 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 abstract 2
- 210000002421 cell wall Anatomy 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 229910021645 metal ion Inorganic materials 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 229920000642 polymer Polymers 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0025—Culture media for plant cell or plant tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
La presente invención se refiere a un método para producir una proteína recombinante en células de plantas, caracterizado porque comprende las etapas de: (a) desarrollar células que tienen una pared celular en un primer medio de cultivo que tiene una osmolaridad de menos de 0.1 osmol/L, en donde el medio no comprende un polímero de superficie activa no iónico o comprende el polímero de superficie activa no iónico en una concentración máxima, por lo que la proteína se acumula en el interior de la célula o en el espacio apoplástico de la célula (b) desarrollar luego las células en un segundo medio de cultivo que contiene una combinación de un polímero de superficie activa no iónico en una concentración mínima y iones de metal monovalente y que tiene una osmolaridad de al menos 0.32 osmol/L, en donde la proteína recombinante es expresada en las células y por lo que la proteína recombinante es secretada a través de la pared celular en el medio, en donde la etapa (a) se lleva a cabo durante un período de al menos 2 días, en donde para el polímero también (i) la concentración máxima de la etapa (a) es 0.08% en peso y la concentración mínima de la etapa (b) es 0.5% en peso, o (ii) la concentración máxima de la etapa (a) es 0.01% en peso y la concentración mínima de la etapa (b) es 0.1% en peso.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12164458.7A EP2653548A1 (de) | 2012-04-17 | 2012-04-17 | Verfahren zur Erhöhung der Sekretion rekombinanter Proteine |
| PCT/EP2013/057956 WO2013156504A1 (de) | 2012-04-17 | 2013-04-17 | Verfahren zur erhöhung der sekretion rekombinanter proteine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2014012407A MX2014012407A (es) | 2015-04-09 |
| MX353243B true MX353243B (es) | 2018-01-05 |
Family
ID=48143287
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014012407A MX353243B (es) | 2012-04-17 | 2013-04-17 | Metodo para incrementar la secrecion de proteinas recombinantes. |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US9850513B2 (es) |
| EP (2) | EP2653548A1 (es) |
| JP (1) | JP6215305B2 (es) |
| KR (1) | KR102028738B1 (es) |
| CN (1) | CN104379750A (es) |
| AU (1) | AU2013248312B2 (es) |
| BR (1) | BR112014025306B1 (es) |
| CA (1) | CA2870587A1 (es) |
| DK (1) | DK2839012T3 (es) |
| ES (1) | ES2755973T3 (es) |
| HR (1) | HRP20191876T1 (es) |
| IL (1) | IL235052B (es) |
| IN (1) | IN2014DN08664A (es) |
| LT (1) | LT2839012T (es) |
| MX (1) | MX353243B (es) |
| PL (1) | PL2839012T3 (es) |
| PT (1) | PT2839012T (es) |
| SI (1) | SI2839012T1 (es) |
| WO (1) | WO2013156504A1 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2729732B2 (ja) | 1992-10-05 | 1998-03-18 | 新日本製鐵株式会社 | 底つき容器の製造方法 |
| EP3808854A1 (de) | 2019-10-17 | 2021-04-21 | eleva GmbH | Verfahren zur gewinnung von material von pflanzenzelloberflächen |
| AU2021213606A1 (en) * | 2020-01-31 | 2022-07-21 | Chugai Seiyaku Kabushiki Kaisha | Method for producing composition containing polypeptide with suppressed coloring |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63226281A (ja) * | 1986-12-26 | 1988-09-20 | Seitai Kinou Riyou Kagakuhin Shinseizou Gijutsu Kenkyu Kumiai | 組織培養方法 |
| JP2000245470A (ja) * | 1999-02-26 | 2000-09-12 | Noda Inst For Scient Res | 植物細胞間液による外来ポリペプチドの製造方法 |
| DE19947290A1 (de) | 1999-10-01 | 2001-04-19 | Greenovation Pflanzenbiotechno | Verfahren zur Herstellung proteinöser Substanzen |
| CN1109750C (zh) * | 2000-08-15 | 2003-05-28 | 中国水产科学研究院黄海水产研究所 | 一种新型低温碱性蛋白酶、制造方法、应用和产生该蛋白酶的微生物 |
| MXPA04009381A (es) | 2002-03-27 | 2005-01-25 | Immunex Corp | Metodos para incrementar la produccion de polipeptidos. |
| EP2154252B1 (en) * | 2008-07-16 | 2014-12-24 | Sumitomo Chemical Company, Limited | Methods for producing secreted proteins |
| US20100035327A1 (en) * | 2008-08-11 | 2010-02-11 | Ann Marie Steele | Use of rice-derived products in a universal cell culture medium |
-
2012
- 2012-04-17 EP EP12164458.7A patent/EP2653548A1/de not_active Withdrawn
-
2013
- 2013-04-17 LT LT13717769T patent/LT2839012T/lt unknown
- 2013-04-17 SI SI201331618T patent/SI2839012T1/sl unknown
- 2013-04-17 MX MX2014012407A patent/MX353243B/es active IP Right Grant
- 2013-04-17 BR BR112014025306-4A patent/BR112014025306B1/pt active IP Right Grant
- 2013-04-17 WO PCT/EP2013/057956 patent/WO2013156504A1/de not_active Ceased
- 2013-04-17 JP JP2015506220A patent/JP6215305B2/ja active Active
- 2013-04-17 HR HRP20191876TT patent/HRP20191876T1/hr unknown
- 2013-04-17 CN CN201380020538.2A patent/CN104379750A/zh active Pending
- 2013-04-17 US US14/394,991 patent/US9850513B2/en active Active
- 2013-04-17 EP EP13717769.7A patent/EP2839012B1/de active Active
- 2013-04-17 KR KR1020147032061A patent/KR102028738B1/ko active Active
- 2013-04-17 AU AU2013248312A patent/AU2013248312B2/en active Active
- 2013-04-17 PL PL13717769T patent/PL2839012T3/pl unknown
- 2013-04-17 PT PT137177697T patent/PT2839012T/pt unknown
- 2013-04-17 IN IN8664DEN2014 patent/IN2014DN08664A/en unknown
- 2013-04-17 ES ES13717769T patent/ES2755973T3/es active Active
- 2013-04-17 CA CA2870587A patent/CA2870587A1/en active Pending
- 2013-04-17 DK DK13717769.7T patent/DK2839012T3/da active
-
2014
- 2014-10-07 IL IL235052A patent/IL235052B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| AU2013248312B2 (en) | 2018-08-30 |
| HRP20191876T1 (hr) | 2019-12-27 |
| WO2013156504A1 (de) | 2013-10-24 |
| JP6215305B2 (ja) | 2017-10-18 |
| EP2839012B1 (de) | 2019-08-28 |
| LT2839012T (lt) | 2019-11-25 |
| KR102028738B1 (ko) | 2019-10-04 |
| KR20150014454A (ko) | 2015-02-06 |
| US20150079632A1 (en) | 2015-03-19 |
| MX2014012407A (es) | 2015-04-09 |
| AU2013248312A1 (en) | 2014-10-23 |
| PL2839012T3 (pl) | 2020-05-18 |
| IL235052B (en) | 2019-12-31 |
| BR112014025306B1 (pt) | 2021-11-30 |
| ES2755973T3 (es) | 2020-04-24 |
| JP2015514408A (ja) | 2015-05-21 |
| DK2839012T3 (da) | 2019-10-21 |
| US9850513B2 (en) | 2017-12-26 |
| CN104379750A (zh) | 2015-02-25 |
| PT2839012T (pt) | 2019-12-05 |
| IL235052A0 (en) | 2014-12-31 |
| EP2653548A1 (de) | 2013-10-23 |
| SI2839012T1 (sl) | 2019-12-31 |
| EP2839012A1 (de) | 2015-02-25 |
| BR112014025306A2 (pt) | 2017-06-20 |
| IN2014DN08664A (es) | 2015-05-22 |
| CA2870587A1 (en) | 2013-10-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |