MX353243B - Metodo para incrementar la secrecion de proteinas recombinantes. - Google Patents
Metodo para incrementar la secrecion de proteinas recombinantes.Info
- Publication number
- MX353243B MX353243B MX2014012407A MX2014012407A MX353243B MX 353243 B MX353243 B MX 353243B MX 2014012407 A MX2014012407 A MX 2014012407A MX 2014012407 A MX2014012407 A MX 2014012407A MX 353243 B MX353243 B MX 353243B
- Authority
- MX
- Mexico
- Prior art keywords
- secretion
- increasing
- recombinant proteins
- cells
- cell wall
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0025—Culture media for plant cell or plant tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
La presente invención se refiere a un método para producir una proteína recombinante en células de plantas, caracterizado porque comprende las etapas de: (a) desarrollar células que tienen una pared celular en un primer medio de cultivo que tiene una osmolaridad de menos de 0.1 osmol/L, en donde el medio no comprende un polímero de superficie activa no iónico o comprende el polímero de superficie activa no iónico en una concentración máxima, por lo que la proteína se acumula en el interior de la célula o en el espacio apoplástico de la célula (b) desarrollar luego las células en un segundo medio de cultivo que contiene una combinación de un polímero de superficie activa no iónico en una concentración mínima y iones de metal monovalente y que tiene una osmolaridad de al menos 0.32 osmol/L, en donde la proteína recombinante es expresada en las células y por lo que la proteína recombinante es secretada a través de la pared celular en el medio, en donde la etapa (a) se lleva a cabo durante un período de al menos 2 días, en donde para el polímero también (i) la concentración máxima de la etapa (a) es 0.08% en peso y la concentración mínima de la etapa (b) es 0.5% en peso, o (ii) la concentración máxima de la etapa (a) es 0.01% en peso y la concentración mínima de la etapa (b) es 0.1% en peso.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12164458.7A EP2653548A1 (de) | 2012-04-17 | 2012-04-17 | Verfahren zur Erhöhung der Sekretion rekombinanter Proteine |
| PCT/EP2013/057956 WO2013156504A1 (de) | 2012-04-17 | 2013-04-17 | Verfahren zur erhöhung der sekretion rekombinanter proteine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2014012407A MX2014012407A (es) | 2015-04-09 |
| MX353243B true MX353243B (es) | 2018-01-05 |
Family
ID=48143287
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014012407A MX353243B (es) | 2012-04-17 | 2013-04-17 | Metodo para incrementar la secrecion de proteinas recombinantes. |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US9850513B2 (es) |
| EP (2) | EP2653548A1 (es) |
| JP (1) | JP6215305B2 (es) |
| KR (1) | KR102028738B1 (es) |
| CN (1) | CN104379750A (es) |
| AU (1) | AU2013248312B2 (es) |
| BR (1) | BR112014025306B1 (es) |
| CA (1) | CA2870587A1 (es) |
| DK (1) | DK2839012T3 (es) |
| ES (1) | ES2755973T3 (es) |
| HR (1) | HRP20191876T1 (es) |
| IL (1) | IL235052B (es) |
| IN (1) | IN2014DN08664A (es) |
| LT (1) | LT2839012T (es) |
| MX (1) | MX353243B (es) |
| PL (1) | PL2839012T3 (es) |
| PT (1) | PT2839012T (es) |
| SI (1) | SI2839012T1 (es) |
| WO (1) | WO2013156504A1 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2729732B2 (ja) | 1992-10-05 | 1998-03-18 | 新日本製鐵株式会社 | 底つき容器の製造方法 |
| EP3808854A1 (de) | 2019-10-17 | 2021-04-21 | eleva GmbH | Verfahren zur gewinnung von material von pflanzenzelloberflächen |
| JP7621284B2 (ja) * | 2020-01-31 | 2025-01-24 | 中外製薬株式会社 | 着色を抑制したポリペプチドを含む組成物の製造方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63226281A (ja) * | 1986-12-26 | 1988-09-20 | Seitai Kinou Riyou Kagakuhin Shinseizou Gijutsu Kenkyu Kumiai | 組織培養方法 |
| JP2000245470A (ja) * | 1999-02-26 | 2000-09-12 | Noda Inst For Scient Res | 植物細胞間液による外来ポリペプチドの製造方法 |
| DE19947290A1 (de) | 1999-10-01 | 2001-04-19 | Greenovation Pflanzenbiotechno | Verfahren zur Herstellung proteinöser Substanzen |
| CN1109750C (zh) * | 2000-08-15 | 2003-05-28 | 中国水产科学研究院黄海水产研究所 | 一种新型低温碱性蛋白酶、制造方法、应用和产生该蛋白酶的微生物 |
| CA2480121C (en) | 2002-03-27 | 2012-02-28 | Immunex Corporation | Methods for increasing polypeptide production |
| US8637316B2 (en) * | 2008-07-16 | 2014-01-28 | Sumitomo Chemical Company, Limited | Glycinin signal sequence for producing secreted proteins in plants |
| US20100035327A1 (en) * | 2008-08-11 | 2010-02-11 | Ann Marie Steele | Use of rice-derived products in a universal cell culture medium |
-
2012
- 2012-04-17 EP EP12164458.7A patent/EP2653548A1/de not_active Withdrawn
-
2013
- 2013-04-17 PT PT137177697T patent/PT2839012T/pt unknown
- 2013-04-17 CN CN201380020538.2A patent/CN104379750A/zh active Pending
- 2013-04-17 KR KR1020147032061A patent/KR102028738B1/ko active Active
- 2013-04-17 BR BR112014025306-4A patent/BR112014025306B1/pt active IP Right Grant
- 2013-04-17 PL PL13717769T patent/PL2839012T3/pl unknown
- 2013-04-17 LT LT13717769T patent/LT2839012T/lt unknown
- 2013-04-17 EP EP13717769.7A patent/EP2839012B1/de active Active
- 2013-04-17 MX MX2014012407A patent/MX353243B/es active IP Right Grant
- 2013-04-17 AU AU2013248312A patent/AU2013248312B2/en active Active
- 2013-04-17 SI SI201331618T patent/SI2839012T1/sl unknown
- 2013-04-17 JP JP2015506220A patent/JP6215305B2/ja active Active
- 2013-04-17 WO PCT/EP2013/057956 patent/WO2013156504A1/de not_active Ceased
- 2013-04-17 DK DK13717769.7T patent/DK2839012T3/da active
- 2013-04-17 IN IN8664DEN2014 patent/IN2014DN08664A/en unknown
- 2013-04-17 US US14/394,991 patent/US9850513B2/en active Active
- 2013-04-17 ES ES13717769T patent/ES2755973T3/es active Active
- 2013-04-17 HR HRP20191876TT patent/HRP20191876T1/hr unknown
- 2013-04-17 CA CA2870587A patent/CA2870587A1/en active Pending
-
2014
- 2014-10-07 IL IL235052A patent/IL235052B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| CN104379750A (zh) | 2015-02-25 |
| PT2839012T (pt) | 2019-12-05 |
| KR102028738B1 (ko) | 2019-10-04 |
| US9850513B2 (en) | 2017-12-26 |
| US20150079632A1 (en) | 2015-03-19 |
| WO2013156504A1 (de) | 2013-10-24 |
| EP2653548A1 (de) | 2013-10-23 |
| LT2839012T (lt) | 2019-11-25 |
| SI2839012T1 (sl) | 2019-12-31 |
| AU2013248312A1 (en) | 2014-10-23 |
| EP2839012B1 (de) | 2019-08-28 |
| DK2839012T3 (da) | 2019-10-21 |
| IL235052B (en) | 2019-12-31 |
| EP2839012A1 (de) | 2015-02-25 |
| IL235052A0 (en) | 2014-12-31 |
| KR20150014454A (ko) | 2015-02-06 |
| BR112014025306B1 (pt) | 2021-11-30 |
| PL2839012T3 (pl) | 2020-05-18 |
| JP6215305B2 (ja) | 2017-10-18 |
| ES2755973T3 (es) | 2020-04-24 |
| MX2014012407A (es) | 2015-04-09 |
| AU2013248312B2 (en) | 2018-08-30 |
| IN2014DN08664A (es) | 2015-05-22 |
| HRP20191876T1 (hr) | 2019-12-27 |
| JP2015514408A (ja) | 2015-05-21 |
| BR112014025306A2 (pt) | 2017-06-20 |
| CA2870587A1 (en) | 2013-10-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MX343604B (es) | Disminuir nivel de lactato e incrementar producción de polipéptidos por regulación a la baja de la expresión de lactato dehidrogenasa y piruvato dehidrogenasa quinasa. | |
| MY181513A (en) | Cell culture compositions and methods for polypeptide production | |
| SG10201809452RA (en) | Cell culture compositions with antioxidants and methods for polypeptide production | |
| WO2008154014A3 (en) | A method for culturing mammalian cells to improve recombinant protein production | |
| WO2012135621A3 (en) | Priming of pluripotent stem cells for neural differentiation | |
| IN2015DN02630A (es) | ||
| EP4148121A3 (en) | Cell-controlled perfusion in continuous culture | |
| WO2010096588A3 (en) | Cell culture media containing combinations of proteins | |
| AR069956A1 (es) | Procesos para cultivar celulas mamiferas secretoras de proteinas sanguineas heterologas | |
| EP2633036A4 (en) | DIFFERENTIATION METHOD FOR THE PRODUCTION OF GLIA CELL POPULATIONS | |
| MX351974B (es) | Cultivo de celulas de mamifero. | |
| SG10201901417UA (en) | Production of proteins in glutamine-free cell culture media | |
| MX348269B (es) | Metodo para producir lamina celular epitelial de pigmento retinal. | |
| MX2015011781A (es) | Medio de cultivo celular y metodos de produccion de anticuerpos. | |
| PH12014501019A1 (en) | Kit comprising serum replacement and labile factors | |
| EP2582792A4 (en) | HEART OF CARDIOMYOCYTES WITH DIALYSATED SERUM | |
| EA201170024A1 (ru) | Способы получения высокой плотности жизнеспособных клеток в культуре клеток млекопитающих | |
| SG10201806083RA (en) | Microcarrier perfusion culturing methods and uses thereof | |
| MY180183A (en) | Methods and compositions for preventing norleucine misincorporation into proteins | |
| WO2011112601A3 (en) | Continuous flow bioreactor for magnetically stabilized three-dimensional tissue culture | |
| MX2021001981A (es) | Metodos para preparar un poloxamero para usar en medio de cultivo celular. | |
| MX2014012407A (es) | Metodo para incrementar la secrecion de proteinas recombinantes. | |
| WO2012106089A3 (en) | 3d cell viability assay | |
| MX351991B (es) | Adicion de hierro para mejorar cultivo celular. | |
| MX2015010887A (es) | Formulaciones y metodos para aumentar la produccion de proteinas recombinantes. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |