MX351991B - Adicion de hierro para mejorar cultivo celular. - Google Patents

Adicion de hierro para mejorar cultivo celular.

Info

Publication number
MX351991B
MX351991B MX2014004735A MX2014004735A MX351991B MX 351991 B MX351991 B MX 351991B MX 2014004735 A MX2014004735 A MX 2014004735A MX 2014004735 A MX2014004735 A MX 2014004735A MX 351991 B MX351991 B MX 351991B
Authority
MX
Mexico
Prior art keywords
cell culture
iron
addition
improve cell
titer
Prior art date
Application number
MX2014004735A
Other languages
English (en)
Other versions
MX2014004735A (es
Inventor
Wang Wenge
Luan Yen-Tung
Drapeau Denis
P Nolan Ryan
Original Assignee
Pfizer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=47324213&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX351991(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Pfizer filed Critical Pfizer
Publication of MX2014004735A publication Critical patent/MX2014004735A/es
Publication of MX351991B publication Critical patent/MX351991B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Reproductive Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a un método para aumentar al menos una de densidad, viabilidad y título celular en un medio de cultivo que comprende etapas de: (a) proporcionar células en un cultivo celular par a iniciar un proceso de cultivo celular en donde el medio de cultivo celular comprende hierro como elemento de traza; y (b) añadir una composición que comprende hierro al medio de cultivo celular durante el proceso de cultivo celular de tal manera que la concentración de hierro en el cultivo celular se incremente durante el curso del proceso de cultivo celular para aumentar la densidad celular, viabilidad y/o título en el cultivo, en donde la composición que comprende hierro se añade el día 3 o después del proceso de cultivo celular.
MX2014004735A 2011-10-21 2012-10-09 Adicion de hierro para mejorar cultivo celular. MX351991B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161550058P 2011-10-21 2011-10-21
PCT/IB2012/055457 WO2013057628A1 (en) 2011-10-21 2012-10-09 Addition of iron to improve cell culture

Publications (2)

Publication Number Publication Date
MX2014004735A MX2014004735A (es) 2014-07-28
MX351991B true MX351991B (es) 2017-11-03

Family

ID=47324213

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014004735A MX351991B (es) 2011-10-21 2012-10-09 Adicion de hierro para mejorar cultivo celular.

Country Status (17)

Country Link
US (1) US20140322758A1 (es)
EP (1) EP2768944B2 (es)
JP (1) JP6400473B2 (es)
KR (2) KR20170021919A (es)
CN (1) CN103890166A (es)
AU (1) AU2012324495B2 (es)
BR (1) BR112014009379A2 (es)
CA (1) CA2852021C (es)
DK (1) DK2768944T3 (es)
ES (1) ES2784146T3 (es)
HK (1) HK1198657A1 (es)
IL (1) IL232171B (es)
MX (1) MX351991B (es)
RU (1) RU2663794C2 (es)
SG (2) SG11201401220SA (es)
WO (1) WO2013057628A1 (es)
ZA (1) ZA201402490B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3601584A1 (en) * 2017-03-31 2020-02-05 Boehringer Ingelheim International GmbH Perfusion medium
EP4368698A1 (en) * 2022-11-09 2024-05-15 Sciperio, Inc. Optimized cell culture medium utilizing iron (iii) citrate as an iron delivery method for the in vitro, bioreactor-centric production of manufactured blood

Family Cites Families (38)

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AU2353384A (en) 1983-01-19 1984-07-26 Genentech Inc. Amplification in eukaryotic host cells
US4713339A (en) 1983-01-19 1987-12-15 Genentech, Inc. Polycistronic expression vector construction
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
JPS6147500A (ja) 1984-08-15 1986-03-07 Res Dev Corp Of Japan キメラモノクロ−ナル抗体及びその製造法
EP0173494A3 (en) 1984-08-27 1987-11-25 The Board Of Trustees Of The Leland Stanford Junior University Chimeric receptors by dna splicing and expression
GB8422238D0 (en) 1984-09-03 1984-10-10 Neuberger M S Chimeric proteins
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
US5166320A (en) 1987-04-22 1992-11-24 University Of Connecticut Carrier system and method for the introduction of genes into mammalian cells
US6291159B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for producing polymers having a preselected activity
JPH0396383A (ja) 1989-09-08 1991-04-22 Riso Kagaku Corp 画像形成装置
DK0417563T3 (da) 1989-09-12 2000-11-06 Hoffmann La Roche TNF-bindende proteiner
DK0494955T3 (da) 1989-10-05 1998-10-26 Optein Inc Cellefri syntese og isolering af hidtil ukendte gener og polypeptider
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DE4115722A1 (de) * 1991-05-14 1992-11-19 Boehringer Mannheim Gmbh Serumfreies medium zur kultivierung von saeugerzellen
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Also Published As

Publication number Publication date
US20140322758A1 (en) 2014-10-30
BR112014009379A2 (pt) 2017-04-18
WO2013057628A1 (en) 2013-04-25
AU2012324495B2 (en) 2016-04-21
IL232171A0 (en) 2014-06-30
DK2768944T3 (da) 2020-04-20
EP2768944A1 (en) 2014-08-27
CN103890166A (zh) 2014-06-25
SG11201401220SA (en) 2014-07-30
EP2768944B2 (en) 2023-11-22
MX2014004735A (es) 2014-07-28
IL232171B (en) 2018-07-31
CA2852021C (en) 2018-08-28
HK1198657A1 (en) 2015-05-22
KR20140077977A (ko) 2014-06-24
CA2852021A1 (en) 2013-04-25
SG10201702454YA (en) 2017-05-30
RU2014115731A (ru) 2015-11-27
JP2014530620A (ja) 2014-11-20
EP2768944B1 (en) 2020-02-26
JP6400473B2 (ja) 2018-10-03
ZA201402490B (en) 2015-11-25
RU2663794C2 (ru) 2018-08-09
AU2012324495A1 (en) 2014-04-24
KR20170021919A (ko) 2017-02-28
ES2784146T3 (es) 2020-09-22

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