HRP20191876T1 - Postupak za povećanje izlučivanja rekombinantnih proteina - Google Patents
Postupak za povećanje izlučivanja rekombinantnih proteina Download PDFInfo
- Publication number
- HRP20191876T1 HRP20191876T1 HRP20191876TT HRP20191876T HRP20191876T1 HR P20191876 T1 HRP20191876 T1 HR P20191876T1 HR P20191876T T HRP20191876T T HR P20191876TT HR P20191876 T HRP20191876 T HR P20191876T HR P20191876 T1 HRP20191876 T1 HR P20191876T1
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- Prior art keywords
- concentration
- ions
- medium
- cell
- active polymer
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims 14
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title claims 4
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title claims 4
- 230000028327 secretion Effects 0.000 title claims 3
- 239000002609 medium Substances 0.000 claims 11
- 210000004027 cell Anatomy 0.000 claims 10
- 229920000642 polymer Polymers 0.000 claims 5
- 229910021645 metal ion Inorganic materials 0.000 claims 4
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims 3
- 229910002651 NO3 Inorganic materials 0.000 claims 3
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims 3
- 229910001424 calcium ion Inorganic materials 0.000 claims 3
- 239000001963 growth medium Substances 0.000 claims 3
- -1 nitrate ions Chemical class 0.000 claims 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 3
- 229910001414 potassium ion Inorganic materials 0.000 claims 3
- 229910001415 sodium ion Inorganic materials 0.000 claims 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims 2
- 210000002421 cell wall Anatomy 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 239000002202 Polyethylene glycol Substances 0.000 claims 1
- 238000009825 accumulation Methods 0.000 claims 1
- 229910001413 alkali metal ion Inorganic materials 0.000 claims 1
- 230000029142 excretion Effects 0.000 claims 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 229920001223 polyethylene glycol Polymers 0.000 claims 1
- 230000003248 secreting effect Effects 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 230000004936 stimulating effect Effects 0.000 claims 1
- 238000004114 suspension culture Methods 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0025—Culture media for plant cell or plant tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Claims (15)
1. Postupak za proizvodnju rekombinantnog proteina u stanicama biljke koje imaju staničnu stijenku, koji obuhvaća korak izlučivanja rekombinantnog proteina kroz staničnu stijenku ekspresijom proteina u biljnim stanicama u mediju za kultiviranje koji sadrži kombinaciju površinski aktivnog polimera, jednovalentne ione metala i ima osmolarnost od najmanje 0,32 osmol/L.
2. Postupak prema patentnom zahtjevu 1, naznačen time što se izlučivanje u medij odvija iz apoplastičnog prostora stanice.
3. Postupak prema bilo kojem zahtjevu 1 ili zahtjevu 2, naznačen time što biljna stanica je stanica biljke ili biljnog tkiva, posebno je poželjna stanica mahovine.
4. Postupak prema bilo kojem od patentnih zahtjeva 1 do 3, naznačen time što se stanica uzgaja u suspenzijskoj kulturi.
5. Postupak prema bilo kojem od patentnih zahtjeva 1 do 4, naznačen time što je ion metala, ion alkalijskog metala, poželjno natrij, i/ili ion metala je prisutan u mediju u koncentraciji od najmanje 20 mM.
6. Postupak prema bilo kojem od patentnih zahtjeva 1 do 5, naznačen time što je površinski aktivni polimer polialkil glikol, poželjno polietilen glikol, i/ili površinski aktivni polimer je prisutan u mediju u koncentraciji od najmanje 0,05 mas. %.
7. Postupak prema bilo kojem od patentnih zahtjeva 1 do 6, naznačen time što medij sadrži nitratne ione, poželjno u koncentraciji od najmanje 0,2 mM, fosfatne ione, poželjno u koncentraciji od najmanje 0,05 mM, sulfatne ione, poželjno u koncentraciji od najmanje 0,02 mM, kalcijeve ione, poželjno u koncentraciji od najmanje 0,1 mM, kalijeve ione, poželjno u koncentraciji od najmanje 0,1 mM, natrijeve ione, poželjno u koncentraciji od najmanje 20 mM, ili njihove kombinacije.
8. Postupak prema bilo kojem od patentnih zahtjeva 1 do 7, naznačen time što, se prije navedenog koraka izlučivanja rekombinantnog proteina u navedenom mediju, stanice uzgajaju u prethodnom koraku u kultivacijskom mediju koji ima osmolarnost manju od 0,1 osmol/L, naročito bez navedenog izlučivanja, što uzrokuje nakupljanje proteina unutar stanice ili u apoplastičnom prostoru stanica.
9. Postupak prema patentnom zahtjevu 8, naznačen time što kultivacijski medij, u navedenom prethodnom koraku, sadrži nitratne ione, poželjno u koncentraciji od najmanje 0,2 mM, fosfatne ione, poželjno u koncentraciji od najmanje 0,05 mM, sulfatne ione, poželjno u koncentraciji od najmanje 0,02 mM, kalcijeve ione, poželjno u koncentraciji od najmanje 0,1 mM, kalijeve ione, poželjno u koncentraciji od najmanje 0,1 mM, ili njihove kombinacije; i/ili sadrži natrijeve ione u koncentraciji od najviše 20 mM.
10. Postupak prema bilo kojem zahtjevu 8 ili zahtjevu 9, naznačen time što kultivacijski medij, u navedenom prethodnom koraku, ne sadrži površinski aktivan polimer ili ga sadrži u koncentraciji od najviše 0,08 mas. %.
11. Postupak prema bilo kojem od patentnih zahtjeva 8 do 10, naznačen time što se navedeni prethodni korak provodi u periodu od 2 do 30 dana.
12. Postupak prema bilo kojem od patentnih zahtjeva 1 do 11, naznačen time što se navedeni korak izlučivanja provodi kroz vrijeme od najmanje 2 dana, poželjno kroz 3 do 120 dana.
13. Kultivacijski medij pogodan za poticanje izlučivanja pomoću postupka prema bilo kojem od patentnih zahtjeva 1 do 12, naznačen time što sadrži površinski aktivni polimer u količini od najmanje 0,05 mas. % i jednovalentne ione metala u koncentraciji od najmanje 20 mM i ima osmolarnost od najmanje 0,32 osmol/L.
14. Kultivacijski medij prema patentnom zahtjevu 13 naznačen time što medij sadrži nitratne ione, poželjno u koncentraciji od najmanje 0,2 mM, fosfatne ione, poželjno u koncentraciji od najmanje 0,05 mM, sulfatne ione, poželjno u koncentraciji od najmanje 0,02 mM, kalcijeve ione, poželjno u koncentraciji od najmanje 0,1 mM, kalijeve ione, poželjno u koncentraciji od najmanje 0,1 mM, natrijeve ione, poželjno u koncentraciji od najmanje 40 mM, ili njihove kombinacije.
15. Smjesa suhog medija za rast za rekonstituciju kultivacijskog medija prema bilo kojem od zahtjevu 13 ili zahtjevu 14.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12164458.7A EP2653548A1 (de) | 2012-04-17 | 2012-04-17 | Verfahren zur Erhöhung der Sekretion rekombinanter Proteine |
| PCT/EP2013/057956 WO2013156504A1 (de) | 2012-04-17 | 2013-04-17 | Verfahren zur erhöhung der sekretion rekombinanter proteine |
| EP13717769.7A EP2839012B1 (de) | 2012-04-17 | 2013-04-17 | Verfahren zur erhöhung der sekretion rekombinanter proteine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20191876T1 true HRP20191876T1 (hr) | 2019-12-27 |
Family
ID=48143287
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20191876TT HRP20191876T1 (hr) | 2012-04-17 | 2019-10-16 | Postupak za povećanje izlučivanja rekombinantnih proteina |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US9850513B2 (hr) |
| EP (2) | EP2653548A1 (hr) |
| JP (1) | JP6215305B2 (hr) |
| KR (1) | KR102028738B1 (hr) |
| CN (1) | CN104379750A (hr) |
| AU (1) | AU2013248312B2 (hr) |
| CA (1) | CA2870587A1 (hr) |
| DK (1) | DK2839012T3 (hr) |
| ES (1) | ES2755973T3 (hr) |
| HR (1) | HRP20191876T1 (hr) |
| IL (1) | IL235052B (hr) |
| IN (1) | IN2014DN08664A (hr) |
| LT (1) | LT2839012T (hr) |
| MX (1) | MX353243B (hr) |
| PL (1) | PL2839012T3 (hr) |
| PT (1) | PT2839012T (hr) |
| SI (1) | SI2839012T1 (hr) |
| WO (1) | WO2013156504A1 (hr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3808854A1 (de) | 2019-10-17 | 2021-04-21 | eleva GmbH | Verfahren zur gewinnung von material von pflanzenzelloberflächen |
| EP4098751A4 (en) * | 2020-01-31 | 2023-06-21 | Chugai Seiyaku Kabushiki Kaisha | METHOD FOR PRODUCING A LESS COLORED COMPOSITION CONTAINING A POLYPEPTIDE |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63226281A (ja) * | 1986-12-26 | 1988-09-20 | Seitai Kinou Riyou Kagakuhin Shinseizou Gijutsu Kenkyu Kumiai | 組織培養方法 |
| JP2000245470A (ja) * | 1999-02-26 | 2000-09-12 | Noda Inst For Scient Res | 植物細胞間液による外来ポリペプチドの製造方法 |
| DE19947290A1 (de) | 1999-10-01 | 2001-04-19 | Greenovation Pflanzenbiotechno | Verfahren zur Herstellung proteinöser Substanzen |
| CN1109750C (zh) * | 2000-08-15 | 2003-05-28 | 中国水产科学研究院黄海水产研究所 | 一种新型低温碱性蛋白酶、制造方法、应用和产生该蛋白酶的微生物 |
| EP1497444B1 (en) | 2002-03-27 | 2015-11-04 | Immunex Corporation | Methods for increasing polypeptide production |
| EP2154252B1 (en) * | 2008-07-16 | 2014-12-24 | Sumitomo Chemical Company, Limited | Methods for producing secreted proteins |
| US20100035327A1 (en) * | 2008-08-11 | 2010-02-11 | Ann Marie Steele | Use of rice-derived products in a universal cell culture medium |
-
2012
- 2012-04-17 EP EP12164458.7A patent/EP2653548A1/de not_active Withdrawn
-
2013
- 2013-04-17 PT PT137177697T patent/PT2839012T/pt unknown
- 2013-04-17 LT LT13717769T patent/LT2839012T/lt unknown
- 2013-04-17 DK DK13717769.7T patent/DK2839012T3/da active
- 2013-04-17 CN CN201380020538.2A patent/CN104379750A/zh active Pending
- 2013-04-17 ES ES13717769T patent/ES2755973T3/es active Active
- 2013-04-17 IN IN8664DEN2014 patent/IN2014DN08664A/en unknown
- 2013-04-17 EP EP13717769.7A patent/EP2839012B1/de active Active
- 2013-04-17 MX MX2014012407A patent/MX353243B/es active IP Right Grant
- 2013-04-17 AU AU2013248312A patent/AU2013248312B2/en active Active
- 2013-04-17 WO PCT/EP2013/057956 patent/WO2013156504A1/de active Application Filing
- 2013-04-17 KR KR1020147032061A patent/KR102028738B1/ko active IP Right Grant
- 2013-04-17 SI SI201331618T patent/SI2839012T1/sl unknown
- 2013-04-17 PL PL13717769T patent/PL2839012T3/pl unknown
- 2013-04-17 CA CA2870587A patent/CA2870587A1/en active Pending
- 2013-04-17 US US14/394,991 patent/US9850513B2/en active Active
- 2013-04-17 JP JP2015506220A patent/JP6215305B2/ja active Active
-
2014
- 2014-10-07 IL IL235052A patent/IL235052B/en active IP Right Grant
-
2019
- 2019-10-16 HR HRP20191876TT patent/HRP20191876T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| IN2014DN08664A (hr) | 2015-05-22 |
| JP2015514408A (ja) | 2015-05-21 |
| AU2013248312B2 (en) | 2018-08-30 |
| MX353243B (es) | 2018-01-05 |
| WO2013156504A1 (de) | 2013-10-24 |
| CA2870587A1 (en) | 2013-10-24 |
| PT2839012T (pt) | 2019-12-05 |
| SI2839012T1 (sl) | 2019-12-31 |
| JP6215305B2 (ja) | 2017-10-18 |
| EP2839012B1 (de) | 2019-08-28 |
| LT2839012T (lt) | 2019-11-25 |
| KR20150014454A (ko) | 2015-02-06 |
| US9850513B2 (en) | 2017-12-26 |
| DK2839012T3 (da) | 2019-10-21 |
| CN104379750A (zh) | 2015-02-25 |
| KR102028738B1 (ko) | 2019-10-04 |
| IL235052A0 (en) | 2014-12-31 |
| US20150079632A1 (en) | 2015-03-19 |
| EP2653548A1 (de) | 2013-10-23 |
| MX2014012407A (es) | 2015-04-09 |
| EP2839012A1 (de) | 2015-02-25 |
| ES2755973T3 (es) | 2020-04-24 |
| IL235052B (en) | 2019-12-31 |
| AU2013248312A1 (en) | 2014-10-23 |
| PL2839012T3 (pl) | 2020-05-18 |
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