WO2012124978A3 - 3차원 세포배양으로 수득한 조정 배지를 유효성분으로 포함하는 허혈성 질환 치료용 약학적 조성물 - Google Patents

3차원 세포배양으로 수득한 조정 배지를 유효성분으로 포함하는 허혈성 질환 치료용 약학적 조성물 Download PDF

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WO2012124978A3
WO2012124978A3 PCT/KR2012/001838 KR2012001838W WO2012124978A3 WO 2012124978 A3 WO2012124978 A3 WO 2012124978A3 KR 2012001838 W KR2012001838 W KR 2012001838W WO 2012124978 A3 WO2012124978 A3 WO 2012124978A3
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conditioned medium
cell culture
pharmaceutical composition
active ingredient
medium obtained
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PCT/KR2012/001838
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French (fr)
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WO2012124978A2 (ko
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김병수
방석호
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서울대학교산학협력단
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Publication of WO2012124978A3 publication Critical patent/WO2012124978A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/03—Coculture with; Conditioned medium produced by non-embryonic pluripotent stem cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Epidemiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Vascular Medicine (AREA)
  • Urology & Nephrology (AREA)
  • Birds (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 성체 줄기세포를 세포 배양용 배지에서 3차원적으로 배양하여 얻은 배양물로부터 수거한 조정 배지를 유효성분으로 포함하는, 허혈성 질환 치료용 약학적 조성물을 제공한다. 본 발명에 따른 조정 배지는 2차원 배양법에 의해 얻어진 조정 배지에 비해 혈관생성 촉진 인자의 농도 및 함량이 매우 높아 허혈성 혈관 질환의 혈관 재생에 유용하게 사용될 수 있다. 또한, 종래 세포 배양 배지 또는 조정 배지에 사용되던 동물 유래 성분과 완충용액 및 지시약 성분들이 없어 임상에 안전하게 적용가능하며, 적은 양으로도 허혈성 혈관 질환 등에 효과적으로 사용될 수 있다.
PCT/KR2012/001838 2011-03-14 2012-03-14 3차원 세포배양으로 수득한 조정 배지를 유효성분으로 포함하는 허혈성 질환 치료용 약학적 조성물 WO2012124978A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0022382 2011-03-14
KR1020110022382A KR101349183B1 (ko) 2011-03-14 2011-03-14 3차원 세포배양으로 수득한 조건 배지를 유효성분으로 포함하는 허혈성 질환 치료용 약학적 조성물

Publications (2)

Publication Number Publication Date
WO2012124978A2 WO2012124978A2 (ko) 2012-09-20
WO2012124978A3 true WO2012124978A3 (ko) 2012-12-27

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PCT/KR2012/001838 WO2012124978A2 (ko) 2011-03-14 2012-03-14 3차원 세포배양으로 수득한 조정 배지를 유효성분으로 포함하는 허혈성 질환 치료용 약학적 조성물

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KR (1) KR101349183B1 (ko)
WO (1) WO2012124978A2 (ko)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101914580B1 (ko) * 2012-03-07 2018-11-06 주식회사 탑셀바이오 줄기세포 컨디션드 배지 분말을 포함하는 뇌신경 또는 척수신경 관련 질환 예방, 개선 또는 치료용 의약 조성물
KR102029358B1 (ko) * 2018-03-16 2019-10-07 주식회사 이에이치엘바이오 줄기세포 배양액을 포함하는 화장료 조성물 및 상기 줄기세포 배양액의 제조방법
KR102249366B1 (ko) * 2018-10-05 2021-05-06 성균관대학교산학협력단 혈관신생 성장인자 분비 촉진용 나노입자, 이를 이용한 조정배지의 제조 방법, 조정배지 및 이를 포함하는 주사제 조성물
KR102283340B1 (ko) * 2020-03-27 2021-07-30 서울대학교산학협력단 심근 직분화를 위한 심장 모사 세포 배양장치 및 이를 이용한 세포 분화 방법
CN111795879A (zh) * 2020-07-14 2020-10-20 青岛大学附属医院 利用脱水套装制作的病理标本在数字化评估系统中的应用
CN113943696A (zh) * 2021-10-29 2022-01-18 华中农业大学 利用猪血管内皮细胞建立缺氧诱导-三维血管生成模型的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070077232A1 (en) * 1999-05-14 2007-04-05 Skinmedica, Inc. Conditioned cell culture medium compositions and methods of use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070077232A1 (en) * 1999-05-14 2007-04-05 Skinmedica, Inc. Conditioned cell culture medium compositions and methods of use

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
SUK HO BHANG ET AL.: "Angiogenesis in ischemic tissue produced by spheroid grafting of human adipose-derived stromal cells", BIOMATERIALS, vol. 32, no. 11, 22 January 2011 (2011-01-22), pages 2734 - 2747 *
WON-SERK KIM ET AL.: "Antiwrinkle effect of adipose-derived stem cell: Activation of dermal fibroblast by secretory factors", JOURNAL OF DERMATOLOGICAL SCIENCE, vol. 53, no. 2, 2009, pages 96 - 102 *
WON-SERK KIM ET AL.: "Wound healing effect of adipose-derived stem cells: A critical role of secretory factors on human dermal fibroblasts", JOURNAL OF DERMATOLOGICAL SCIENCE, vol. 48, no. 1, 2007, pages 15 - 24 *

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KR101349183B1 (ko) 2014-01-08
WO2012124978A2 (ko) 2012-09-20
KR20120104771A (ko) 2012-09-24

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