BR112015022770A8 - meio e sistema de cultivo da célula livre de xenógeno, livre de albumina, forma concentrada, métodos de diferenciação de células-tronco pluripotentes humanas, para a produção de mesenquimoangioblastos e sistema definido de cultivo da célula para diferenciação hematoendotelial das células- tronco pluripotentes humanas - Google Patents
meio e sistema de cultivo da célula livre de xenógeno, livre de albumina, forma concentrada, métodos de diferenciação de células-tronco pluripotentes humanas, para a produção de mesenquimoangioblastos e sistema definido de cultivo da célula para diferenciação hematoendotelial das células- tronco pluripotentes humanas Download PDFInfo
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- BR112015022770A8 BR112015022770A8 BR112015022770A BR112015022770A BR112015022770A8 BR 112015022770 A8 BR112015022770 A8 BR 112015022770A8 BR 112015022770 A BR112015022770 A BR 112015022770A BR 112015022770 A BR112015022770 A BR 112015022770A BR 112015022770 A8 BR112015022770 A8 BR 112015022770A8
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- pluripotent stem
- human pluripotent
- stem cells
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- 210000001778 pluripotent stem cell Anatomy 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 4
- 230000004069 differentiation Effects 0.000 title abstract 3
- 238000004113 cell culture Methods 0.000 title 1
- 239000006143 cell culture medium Substances 0.000 title 1
- 230000024245 cell differentiation Effects 0.000 title 1
- 239000000203 mixture Substances 0.000 abstract 2
- 102000005606 Activins Human genes 0.000 abstract 1
- 108010059616 Activins Proteins 0.000 abstract 1
- 101150063837 Aplnr gene Proteins 0.000 abstract 1
- 101150019331 FGF2 gene Proteins 0.000 abstract 1
- 206010021143 Hypoxia Diseases 0.000 abstract 1
- 239000000488 activin Substances 0.000 abstract 1
- 101150067309 bmp4 gene Proteins 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000003511 endothelial effect Effects 0.000 abstract 1
- YSFTYXKQUONNFY-NQXPEFQPSA-N fgf2 Chemical compound C([C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](C)NC(=O)CNC(=O)[C@H](CO)NC(=O)CNC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H]1NCCC1)C1=CC=CC=C1 YSFTYXKQUONNFY-NQXPEFQPSA-N 0.000 abstract 1
- 230000003394 haemopoietic effect Effects 0.000 abstract 1
- 230000001146 hypoxic effect Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 210000001704 mesoblast Anatomy 0.000 abstract 1
- 210000000130 stem cell Anatomy 0.000 abstract 1
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Abstract
"métodos e materiais para diferenciação hematoendotelial de células-tronco pluripotentes humanas sob condições definidas". o presente pedido de patente refere-se a métodos e composições para a diferenciação de células tronco pluripotentes para células de linhagens hematopoiéticas e endoteliais são divulgados. particularmente, refere-se a um método de diferenciação de células tronco pluripotentes humanas, compreendendo as etapas de (a) forncecer células tronco pluripotentes humanas; e (b) expor as células tronco a uma mistura compreendendo componentes fgf2, bmp4, activina a, e licl, sob condições hipóxicas durante um período de cerca de dois dias de modo a formar uma população de células da mesoderme primitivas emhlin- kdr+aplnr+pdgfralpha+ com potencial mesenquimoangioblasto.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361779564P | 2013-03-13 | 2013-03-13 | |
US61/779,564 | 2013-03-13 | ||
PCT/US2014/024518 WO2014165131A1 (en) | 2013-03-13 | 2014-03-12 | Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions |
Publications (3)
Publication Number | Publication Date |
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BR112015022770A2 BR112015022770A2 (pt) | 2017-07-18 |
BR112015022770A8 true BR112015022770A8 (pt) | 2019-11-26 |
BR112015022770B1 BR112015022770B1 (pt) | 2022-11-16 |
Family
ID=50678273
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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BR112015022770-8A BR112015022770B1 (pt) | 2013-03-13 | 2014-03-12 | Meio e sistema de cultivo da célula livre de xenógeno, livre de albumina, forma concentrada, métodos de diferenciação de células-tronco pluripotentes humanas, para a produção de mesenquimoangioblastos e sistema definido de cultivo da célula para diferenciação hematoendotelial das células- tronco pluripotentes humanas |
Country Status (19)
Country | Link |
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US (3) | US9938499B2 (pt) |
EP (1) | EP2970912B1 (pt) |
JP (1) | JP6677631B2 (pt) |
KR (1) | KR102151210B1 (pt) |
CN (1) | CN105209609B (pt) |
AU (1) | AU2014248651B2 (pt) |
BR (1) | BR112015022770B1 (pt) |
CA (2) | CA3141731A1 (pt) |
DK (1) | DK2970912T3 (pt) |
ES (1) | ES2720196T3 (pt) |
HK (1) | HK1218927A1 (pt) |
HU (1) | HUE043785T2 (pt) |
IL (1) | IL241286B (pt) |
MX (1) | MX366900B (pt) |
PL (1) | PL2970912T3 (pt) |
PT (1) | PT2970912T (pt) |
SG (1) | SG11201507275TA (pt) |
TR (1) | TR201904270T4 (pt) |
WO (1) | WO2014165131A1 (pt) |
Families Citing this family (64)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3712258A1 (en) | 2006-04-14 | 2020-09-23 | Astellas Institute for Regenerative Medicine | Hemangio-colony forming cells |
WO2011140441A2 (en) | 2010-05-06 | 2011-11-10 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into intestinal tissues through directed differentiation |
US9868939B2 (en) | 2013-06-12 | 2018-01-16 | Wisconsin Alumni Research Foundation | Generating vasculogenic cell populations |
EP3712254A1 (en) | 2014-05-28 | 2020-09-23 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into gastric tissues through directed differentiation |
JP6804438B2 (ja) | 2014-10-17 | 2020-12-23 | チルドレンズ ホスピタル メディカル センター | 多能性幹細胞を使用するヒト小腸のin vivoモデル、並びにそれを作製、及び使用する方法 |
US11220672B2 (en) * | 2014-12-31 | 2022-01-11 | Wisconsin Alumni Research Foundation | Human pluripotent stem cell-based system for generating endothelial cells |
SG10202101358XA (en) * | 2015-01-26 | 2021-03-30 | Fate Therapeutics Inc | Methods and compositions for inducing hematopoietic cell differentiation |
AU2016219815B2 (en) | 2015-02-20 | 2022-03-10 | Wisconsin Alumni Research Foundation | Generating arterial endothelial cell populations |
US10787640B2 (en) | 2015-03-03 | 2020-09-29 | The Board Of Trustees Of The Leland Stanford Junior University | Producing mesodermal cell types and methods of using the same |
CN104660703B (zh) * | 2015-03-05 | 2017-12-15 | 国家电网公司 | 多网络融合用电信息采集系统及最优通信网络的选择方法 |
WO2016143826A1 (ja) * | 2015-03-09 | 2016-09-15 | 学校法人慶應義塾 | 多能性幹細胞を所望の細胞型へ分化する方法 |
WO2017004169A1 (en) * | 2015-06-29 | 2017-01-05 | Northeastern University | Dendritic cell generator |
JP6646311B2 (ja) * | 2015-07-17 | 2020-02-14 | 国立大学法人京都大学 | 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法 |
ES2862676T3 (es) * | 2015-10-20 | 2021-10-07 | Fujifilm Cellular Dynamics Inc | Producción de células precursoras hematopoyéticas multilinaje mediante programación genética |
CN115927199A (zh) * | 2015-11-04 | 2023-04-07 | 菲特治疗公司 | 用于诱导造血细胞分化的方法和组合物 |
CN108368520B (zh) | 2015-11-04 | 2023-01-17 | 菲特治疗公司 | 多能细胞的基因组工程改造 |
AU2017234378B2 (en) | 2016-03-16 | 2022-10-13 | Cynata Therapeutics Limited | Colony forming medium and use thereof |
US20190127760A1 (en) * | 2016-04-15 | 2019-05-02 | Angiocrine Bioscience, Inc. | Enhanced gene delivery methods |
WO2017192997A1 (en) | 2016-05-05 | 2017-11-09 | Children's Hospital Medical Center | Methods for the in vitro manufacture of gastric fundus tissue and compositions related to same |
WO2017200486A1 (en) * | 2016-05-17 | 2017-11-23 | Agency For Science, Technology And Research | Human stem cell derived endothelial cells, endothelial- hepatocyte co-culture system and uses thereof |
WO2018037091A1 (en) * | 2016-08-24 | 2018-03-01 | Hi-Stem Ggmbh | Methods for the identification and isolation of hematopoietic stem and progenitor cells |
JP7215994B2 (ja) | 2016-09-06 | 2023-01-31 | ザ チルドレンズ メディカル センター コーポレーション | 人工多能性幹細胞由来の免疫細胞 |
US10961505B2 (en) | 2016-10-05 | 2021-03-30 | FUJIFILM Cellular Dynamics, Inc. | Generating mature lineages from induced pluripotent stem cells with MECP2 disruption |
JP7212615B2 (ja) * | 2016-10-05 | 2023-01-25 | フジフィルム セルラー ダイナミクス,インコーポレイテッド | 多能性幹細胞からhlaホモ接合免疫細胞への指向分化方法 |
KR102558606B1 (ko) | 2016-12-05 | 2023-07-26 | 칠드런즈 호스피탈 메디칼 센터 | 결장 유사장기 및 이를 제조 및 사용하는 방법 |
KR102660814B1 (ko) * | 2017-03-21 | 2024-04-26 | 에따블리스망 프랑스와 뒤 상 | 조혈 이식체의 개선 방법 |
CN106867961A (zh) * | 2017-03-27 | 2017-06-20 | 广州润虹医药科技有限公司 | 一种诱导多能干细胞形成中胚层细胞的诱导培养基及诱导方法 |
JP2020516605A (ja) * | 2017-04-07 | 2020-06-11 | サイナータ セラピューティクス リミテッド | キメラ抗原受容体(car)t細胞療法の副作用を治療する方法 |
MX2019014867A (es) * | 2017-06-16 | 2020-02-13 | Cynata Therapeutics Ltd | Metodo para tratar un efecto secundario de la inmunoterapia. |
EP3638773A1 (en) * | 2017-06-16 | 2020-04-22 | IMBA-Institut für Molekulare Biotechnologie GmbH | Blood vessel organoid, methods of producing and using said organoids |
WO2019051281A1 (en) * | 2017-09-07 | 2019-03-14 | StemoniX Inc. | EFFECTIVE DIFFERENTIATION OF HUMAN STEM CELLS IN DEFINITIVE ENDODERM |
SG11202001909TA (en) | 2017-09-15 | 2020-04-29 | Cynata Therapeutics Ltd | Method for treating allergic airways disease (aad)/ asthma |
CN111433351A (zh) | 2017-10-31 | 2020-07-17 | 默多克儿童研究所 | 组合物和方法 |
WO2019094614A1 (en) | 2017-11-08 | 2019-05-16 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Methods of preparing hematopoietic progenitor cells in vitro |
CN112004923A (zh) | 2017-11-22 | 2020-11-27 | 迈索布拉斯特国际有限公司 | 细胞组合物及治疗方法 |
WO2019126146A1 (en) | 2017-12-19 | 2019-06-27 | Xcell Biosciences, Inc. | Methods of modulating cell phenotype by way of regulating the gaseous environment |
KR102592176B1 (ko) * | 2018-01-05 | 2023-10-20 | 플레이틀렛 바이오제네시스, 인크. | 거핵구 생성을 위한 조성물 및 방법 |
EP3847243A1 (en) * | 2018-09-07 | 2021-07-14 | Wisconsin Alumni Research Foundation | Generation of hematopoietic progenitor cells from human pluripotent stem cells |
CN109334379B (zh) * | 2018-09-19 | 2021-11-23 | 江苏大学 | 重型车用液电式惯容器非线性惯性力辨识方法 |
US10647954B1 (en) | 2018-11-15 | 2020-05-12 | Flaskworks, Llc | Dendritic cell generating apparatus and method |
US20220025330A1 (en) * | 2019-01-22 | 2022-01-27 | Washington University | Compositions and methods for generating hematopoietic stem cells (hscs) |
CN111154719B (zh) * | 2019-02-28 | 2024-03-01 | 西湖生物医药科技(杭州)有限公司 | 利用外周血体外制备成熟红细胞的方法及制剂 |
JP2022543445A (ja) | 2019-08-05 | 2022-10-12 | メゾブラスト・インターナショナル・エスアーエールエル | ウイルスベクターを含む細胞組成物及び処置方法 |
US11566217B2 (en) | 2019-08-13 | 2023-01-31 | Flaskworks, Llc | Duty cycle for cell culture systems |
CA3150037A1 (en) * | 2019-08-20 | 2021-02-25 | Adaptimmune Limited | Culture medium for haematopoietic induction |
GB201911953D0 (en) * | 2019-08-20 | 2019-10-02 | Adaptimmune Ltd | T cell production fram RAG inacivated iPSCs |
GB201911957D0 (en) * | 2019-08-20 | 2019-10-02 | Adaptimmune Ltd | Methods of producing haemogenic progenitor cells from pluripotent stem cells |
AU2020337929A1 (en) * | 2019-08-28 | 2022-03-24 | Astellas Institute For Regenerative Medicine | Methods of treating vascular diseases |
JP2022552724A (ja) | 2019-10-21 | 2022-12-19 | フラスクワークス, エルエルシー | 細胞培養のためのシステムおよび方法 |
WO2021085462A1 (ja) * | 2019-10-29 | 2021-05-06 | 国立大学法人京都大学 | 多能性幹細胞から造血性内皮細胞および/または造血前駆細胞を製造する方法 |
WO2021126841A1 (en) * | 2019-12-16 | 2021-06-24 | The Board Of Trustees Of The Leland Stanford Junior University | Use of polyvinyl alcohol for cell culture of immune cells |
JP7072756B2 (ja) * | 2019-12-27 | 2022-05-23 | 国立大学法人京都大学 | 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法 |
CN113493767B (zh) * | 2020-04-01 | 2024-04-26 | 北京大学 | 利用人多潜能干细胞体外制备嗜酸性粒细胞 |
AU2021324483A1 (en) | 2020-08-10 | 2023-04-13 | Mesoblast International Sàrl | Cellular compositions and methods of treatment |
CA3190402A1 (en) * | 2020-08-25 | 2022-03-03 | Gordon Keller | Compositions and methods for generating human yolk sac-like hematopoietic cells |
US11591381B2 (en) | 2020-11-30 | 2023-02-28 | Crispr Therapeutics Ag | Gene-edited natural killer cells |
EP4271798A1 (en) | 2020-12-30 | 2023-11-08 | CRISPR Therapeutics AG | Compositions and methods for differentiating stem cells into nk cells |
CN112680410B (zh) * | 2021-01-19 | 2022-08-02 | 上海爱萨尔生物科技有限公司 | 诱导多能干细胞分化培养心脏成纤维细胞的方法及其培养液 |
EP4293107A1 (en) * | 2021-02-05 | 2023-12-20 | Sungkwang Medical Foundation | Method for differentiating pluripotent stem cell-derived hemogenic endothelial cells into lymphoid lineage cells |
KR20220113586A (ko) * | 2021-02-05 | 2022-08-16 | 의료법인 성광의료재단 | 다능성 줄기세포 유래 조혈 내피세포를 이용한 유전자 편집된 세포의 제조방법 |
GB202102297D0 (en) * | 2021-02-18 | 2021-04-07 | Adaptimmune Ltd | Methods of Producing Haemogenic Endothelial Cells from Pluripotent Stem Cells |
CN113046318B (zh) * | 2021-04-13 | 2023-04-18 | 中国科学院深圳先进技术研究院 | 一种诱导多能干细胞向造血前体细胞分化的培养基以及方法 |
CN117836405A (zh) * | 2021-08-13 | 2024-04-05 | 先声创新公司 | 三维培养多能干细胞产生造血干细胞 |
WO2023201361A1 (en) | 2022-04-15 | 2023-10-19 | Aspen Neuroscience, Inc. | Methods of classifying the differentiation state of cells and related compositions of differentiated cells |
Family Cites Families (13)
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NZ553241A (en) | 2004-09-08 | 2009-11-27 | Wisconsin Alumni Res Found | Medium and culture of pluripotent stem cells |
US8034613B2 (en) | 2005-06-01 | 2011-10-11 | Wisconsin Alumni Research Foundation | Multipotent lymphohematopoietic progenitor cells |
CA2610243C (en) | 2005-06-01 | 2016-11-15 | Wisconsin Alumni Research Foundation | Method of forming dendritic cells from embryonic stem cells |
GB2449042B (en) | 2006-02-09 | 2010-07-07 | Wisconsin Alumni Res Found | Erythroid cells producing adult-type b-hemoglobin generated from human embryonic stem cells |
AU2008231020B2 (en) | 2007-03-23 | 2013-09-05 | Wisconsin Alumni Research Foundation | Somatic cell reprogramming |
US20110236971A2 (en) | 2007-09-25 | 2011-09-29 | Maksym Vodyanyk | Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions |
US7615374B2 (en) | 2007-09-25 | 2009-11-10 | Wisconsin Alumni Research Foundation | Generation of clonal mesenchymal progenitors and mesenchymal stem cell lines under serum-free conditions |
WO2009072990A1 (en) | 2007-12-06 | 2009-06-11 | Agency For Science, Technology And Research | Method for extracellular matrix mediated differentiation and proliferation of stem cells |
MX2010012089A (es) * | 2008-05-06 | 2011-07-28 | Advanced Cell Tech Inc | Metodos para producir celulas eritroides enucleadas derivadas de celulas troncales pluripotentes. |
US8372642B2 (en) | 2009-02-27 | 2013-02-12 | Cellular Dynamics International, Inc. | Differentiation of pluripotent cells |
WO2010143529A1 (ja) * | 2009-06-09 | 2010-12-16 | 国立大学法人名古屋大学 | 多能性幹細胞を胸腺上皮へ分化誘導する方法 |
JP2013524836A (ja) * | 2010-04-25 | 2013-06-20 | マウント・シナイ・スクール・オブ・メディスン | 多能性細胞からの前部前腸内胚葉の生成 |
US9279107B2 (en) | 2010-08-05 | 2016-03-08 | Wisconsin Alumni Research Foundation | Simplified basic media for human pluripotent cell culture |
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US20140273211A1 (en) | 2014-09-18 |
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AU2014248651B2 (en) | 2019-01-17 |
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US9938499B2 (en) | 2018-04-10 |
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SG11201507275TA (en) | 2015-10-29 |
JP2016512031A (ja) | 2016-04-25 |
ES2720196T3 (es) | 2019-07-18 |
JP6677631B2 (ja) | 2020-04-08 |
CA3141731A1 (en) | 2014-10-09 |
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