BR112015022770A8 - meio e sistema de cultivo da célula livre de xenógeno, livre de albumina, forma concentrada, métodos de diferenciação de células-tronco pluripotentes humanas, para a produção de mesenquimoangioblastos e sistema definido de cultivo da célula para diferenciação hematoendotelial das células- tronco pluripotentes humanas - Google Patents

meio e sistema de cultivo da célula livre de xenógeno, livre de albumina, forma concentrada, métodos de diferenciação de células-tronco pluripotentes humanas, para a produção de mesenquimoangioblastos e sistema definido de cultivo da célula para diferenciação hematoendotelial das células- tronco pluripotentes humanas Download PDF

Info

Publication number
BR112015022770A8
BR112015022770A8 BR112015022770A BR112015022770A BR112015022770A8 BR 112015022770 A8 BR112015022770 A8 BR 112015022770A8 BR 112015022770 A BR112015022770 A BR 112015022770A BR 112015022770 A BR112015022770 A BR 112015022770A BR 112015022770 A8 BR112015022770 A8 BR 112015022770A8
Authority
BR
Brazil
Prior art keywords
pluripotent stem
human pluripotent
stem cells
free
cell culture
Prior art date
Application number
BR112015022770A
Other languages
English (en)
Other versions
BR112015022770A2 (pt
BR112015022770B1 (pt
Inventor
Ichiro Uenishi Gene
Slukvin Igor
Original Assignee
Wisconsin Alumni Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Publication of BR112015022770A2 publication Critical patent/BR112015022770A2/pt
Publication of BR112015022770A8 publication Critical patent/BR112015022770A8/pt
Publication of BR112015022770B1 publication Critical patent/BR112015022770B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0056—Xeno-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0695—Stem cells; Progenitor cells; Precursor cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/165—Vascular endothelial growth factor [VEGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1394—Bone marrow stromal cells; whole marrow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Vascular Medicine (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

"métodos e materiais para diferenciação hematoendotelial de células-tronco pluripotentes humanas sob condições definidas". o presente pedido de patente refere-se a métodos e composições para a diferenciação de células tronco pluripotentes para células de linhagens hematopoiéticas e endoteliais são divulgados. particularmente, refere-se a um método de diferenciação de células tronco pluripotentes humanas, compreendendo as etapas de (a) forncecer células tronco pluripotentes humanas; e (b) expor as células tronco a uma mistura compreendendo componentes fgf2, bmp4, activina a, e licl, sob condições hipóxicas durante um período de cerca de dois dias de modo a formar uma população de células da mesoderme primitivas emhlin- kdr+aplnr+pdgfralpha+ com potencial mesenquimoangioblasto.
BR112015022770-8A 2013-03-13 2014-03-12 Meio e sistema de cultivo da célula livre de xenógeno, livre de albumina, forma concentrada, métodos de diferenciação de células-tronco pluripotentes humanas, para a produção de mesenquimoangioblastos e sistema definido de cultivo da célula para diferenciação hematoendotelial das células- tronco pluripotentes humanas BR112015022770B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361779564P 2013-03-13 2013-03-13
US61/779,564 2013-03-13
PCT/US2014/024518 WO2014165131A1 (en) 2013-03-13 2014-03-12 Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions

Publications (3)

Publication Number Publication Date
BR112015022770A2 BR112015022770A2 (pt) 2017-07-18
BR112015022770A8 true BR112015022770A8 (pt) 2019-11-26
BR112015022770B1 BR112015022770B1 (pt) 2022-11-16

Family

ID=50678273

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015022770-8A BR112015022770B1 (pt) 2013-03-13 2014-03-12 Meio e sistema de cultivo da célula livre de xenógeno, livre de albumina, forma concentrada, métodos de diferenciação de células-tronco pluripotentes humanas, para a produção de mesenquimoangioblastos e sistema definido de cultivo da célula para diferenciação hematoendotelial das células- tronco pluripotentes humanas

Country Status (19)

Country Link
US (3) US9938499B2 (pt)
EP (1) EP2970912B1 (pt)
JP (1) JP6677631B2 (pt)
KR (1) KR102151210B1 (pt)
CN (1) CN105209609B (pt)
AU (1) AU2014248651B2 (pt)
BR (1) BR112015022770B1 (pt)
CA (2) CA3141731A1 (pt)
DK (1) DK2970912T3 (pt)
ES (1) ES2720196T3 (pt)
HK (1) HK1218927A1 (pt)
HU (1) HUE043785T2 (pt)
IL (1) IL241286B (pt)
MX (1) MX366900B (pt)
PL (1) PL2970912T3 (pt)
PT (1) PT2970912T (pt)
SG (1) SG11201507275TA (pt)
TR (1) TR201904270T4 (pt)
WO (1) WO2014165131A1 (pt)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3712258A1 (en) 2006-04-14 2020-09-23 Astellas Institute for Regenerative Medicine Hemangio-colony forming cells
WO2011140441A2 (en) 2010-05-06 2011-11-10 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
US9868939B2 (en) 2013-06-12 2018-01-16 Wisconsin Alumni Research Foundation Generating vasculogenic cell populations
EP3712254A1 (en) 2014-05-28 2020-09-23 Children's Hospital Medical Center Methods and systems for converting precursor cells into gastric tissues through directed differentiation
JP6804438B2 (ja) 2014-10-17 2020-12-23 チルドレンズ ホスピタル メディカル センター 多能性幹細胞を使用するヒト小腸のｉｎ ｖｉｖｏモデル、並びにそれを作製、及び使用する方法
US11220672B2 (en) * 2014-12-31 2022-01-11 Wisconsin Alumni Research Foundation Human pluripotent stem cell-based system for generating endothelial cells
SG10202101358XA (en) * 2015-01-26 2021-03-30 Fate Therapeutics Inc Methods and compositions for inducing hematopoietic cell differentiation
AU2016219815B2 (en) 2015-02-20 2022-03-10 Wisconsin Alumni Research Foundation Generating arterial endothelial cell populations
US10787640B2 (en) 2015-03-03 2020-09-29 The Board Of Trustees Of The Leland Stanford Junior University Producing mesodermal cell types and methods of using the same
CN104660703B (zh) * 2015-03-05 2017-12-15 国家电网公司 多网络融合用电信息采集系统及最优通信网络的选择方法
WO2016143826A1 (ja) * 2015-03-09 2016-09-15 学校法人慶應義塾 多能性幹細胞を所望の細胞型へ分化する方法
WO2017004169A1 (en) * 2015-06-29 2017-01-05 Northeastern University Dendritic cell generator
JP6646311B2 (ja) * 2015-07-17 2020-02-14 国立大学法人京都大学 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法
ES2862676T3 (es) * 2015-10-20 2021-10-07 Fujifilm Cellular Dynamics Inc Producción de células precursoras hematopoyéticas multilinaje mediante programación genética
CN115927199A (zh) * 2015-11-04 2023-04-07 菲特治疗公司 用于诱导造血细胞分化的方法和组合物
CN108368520B (zh) 2015-11-04 2023-01-17 菲特治疗公司 多能细胞的基因组工程改造
AU2017234378B2 (en) 2016-03-16 2022-10-13 Cynata Therapeutics Limited Colony forming medium and use thereof
US20190127760A1 (en) * 2016-04-15 2019-05-02 Angiocrine Bioscience, Inc. Enhanced gene delivery methods
WO2017192997A1 (en) 2016-05-05 2017-11-09 Children's Hospital Medical Center Methods for the in vitro manufacture of gastric fundus tissue and compositions related to same
WO2017200486A1 (en) * 2016-05-17 2017-11-23 Agency For Science, Technology And Research Human stem cell derived endothelial cells, endothelial- hepatocyte co-culture system and uses thereof
WO2018037091A1 (en) * 2016-08-24 2018-03-01 Hi-Stem Ggmbh Methods for the identification and isolation of hematopoietic stem and progenitor cells
JP7215994B2 (ja) 2016-09-06 2023-01-31 ザ チルドレンズ メディカル センター コーポレーション 人工多能性幹細胞由来の免疫細胞
US10961505B2 (en) 2016-10-05 2021-03-30 FUJIFILM Cellular Dynamics, Inc. Generating mature lineages from induced pluripotent stem cells with MECP2 disruption
JP7212615B2 (ja) * 2016-10-05 2023-01-25 フジフィルム セルラー ダイナミクス，インコーポレイテッド 多能性幹細胞からｈｌａホモ接合免疫細胞への指向分化方法
KR102558606B1 (ko) 2016-12-05 2023-07-26 칠드런즈 호스피탈 메디칼 센터 결장 유사장기 및 이를 제조 및 사용하는 방법
KR102660814B1 (ko) * 2017-03-21 2024-04-26 에따블리스망 프랑스와 뒤 상 조혈 이식체의 개선 방법
CN106867961A (zh) * 2017-03-27 2017-06-20 广州润虹医药科技有限公司 一种诱导多能干细胞形成中胚层细胞的诱导培养基及诱导方法
JP2020516605A (ja) * 2017-04-07 2020-06-11 サイナータ セラピューティクス リミテッド キメラ抗原受容体（ｃａｒ）ｔ細胞療法の副作用を治療する方法
MX2019014867A (es) * 2017-06-16 2020-02-13 Cynata Therapeutics Ltd Metodo para tratar un efecto secundario de la inmunoterapia.
EP3638773A1 (en) * 2017-06-16 2020-04-22 IMBA-Institut für Molekulare Biotechnologie GmbH Blood vessel organoid, methods of producing and using said organoids
WO2019051281A1 (en) * 2017-09-07 2019-03-14 StemoniX Inc. EFFECTIVE DIFFERENTIATION OF HUMAN STEM CELLS IN DEFINITIVE ENDODERM
SG11202001909TA (en) 2017-09-15 2020-04-29 Cynata Therapeutics Ltd Method for treating allergic airways disease (aad)/ asthma
CN111433351A (zh) 2017-10-31 2020-07-17 默多克儿童研究所 组合物和方法
WO2019094614A1 (en) 2017-11-08 2019-05-16 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods of preparing hematopoietic progenitor cells in vitro
CN112004923A (zh) 2017-11-22 2020-11-27 迈索布拉斯特国际有限公司 细胞组合物及治疗方法
WO2019126146A1 (en) 2017-12-19 2019-06-27 Xcell Biosciences, Inc. Methods of modulating cell phenotype by way of regulating the gaseous environment
KR102592176B1 (ko) * 2018-01-05 2023-10-20 플레이틀렛 바이오제네시스, 인크. 거핵구 생성을 위한 조성물 및 방법
EP3847243A1 (en) * 2018-09-07 2021-07-14 Wisconsin Alumni Research Foundation Generation of hematopoietic progenitor cells from human pluripotent stem cells
CN109334379B (zh) * 2018-09-19 2021-11-23 江苏大学 重型车用液电式惯容器非线性惯性力辨识方法
US10647954B1 (en) 2018-11-15 2020-05-12 Flaskworks, Llc Dendritic cell generating apparatus and method
US20220025330A1 (en) * 2019-01-22 2022-01-27 Washington University Compositions and methods for generating hematopoietic stem cells (hscs)
CN111154719B (zh) * 2019-02-28 2024-03-01 西湖生物医药科技(杭州)有限公司 利用外周血体外制备成熟红细胞的方法及制剂
JP2022543445A (ja) 2019-08-05 2022-10-12 メゾブラスト・インターナショナル・エスアーエールエル ウイルスベクターを含む細胞組成物及び処置方法
US11566217B2 (en) 2019-08-13 2023-01-31 Flaskworks, Llc Duty cycle for cell culture systems
CA3150037A1 (en) * 2019-08-20 2021-02-25 Adaptimmune Limited Culture medium for haematopoietic induction
GB201911953D0 (en) * 2019-08-20 2019-10-02 Adaptimmune Ltd T cell production fram RAG inacivated iPSCs
GB201911957D0 (en) * 2019-08-20 2019-10-02 Adaptimmune Ltd Methods of producing haemogenic progenitor cells from pluripotent stem cells
AU2020337929A1 (en) * 2019-08-28 2022-03-24 Astellas Institute For Regenerative Medicine Methods of treating vascular diseases
JP2022552724A (ja) 2019-10-21 2022-12-19 フラスクワークス， エルエルシー 細胞培養のためのシステムおよび方法
WO2021085462A1 (ja) * 2019-10-29 2021-05-06 国立大学法人京都大学 多能性幹細胞から造血性内皮細胞および／または造血前駆細胞を製造する方法
WO2021126841A1 (en) * 2019-12-16 2021-06-24 The Board Of Trustees Of The Leland Stanford Junior University Use of polyvinyl alcohol for cell culture of immune cells
JP7072756B2 (ja) * 2019-12-27 2022-05-23 国立大学法人京都大学 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法
CN113493767B (zh) * 2020-04-01 2024-04-26 北京大学 利用人多潜能干细胞体外制备嗜酸性粒细胞
AU2021324483A1 (en) 2020-08-10 2023-04-13 Mesoblast International Sàrl Cellular compositions and methods of treatment
CA3190402A1 (en) * 2020-08-25 2022-03-03 Gordon Keller Compositions and methods for generating human yolk sac-like hematopoietic cells
US11591381B2 (en) 2020-11-30 2023-02-28 Crispr Therapeutics Ag Gene-edited natural killer cells
EP4271798A1 (en) 2020-12-30 2023-11-08 CRISPR Therapeutics AG Compositions and methods for differentiating stem cells into nk cells
CN112680410B (zh) * 2021-01-19 2022-08-02 上海爱萨尔生物科技有限公司 诱导多能干细胞分化培养心脏成纤维细胞的方法及其培养液
EP4293107A1 (en) * 2021-02-05 2023-12-20 Sungkwang Medical Foundation Method for differentiating pluripotent stem cell-derived hemogenic endothelial cells into lymphoid lineage cells
KR20220113586A (ko) * 2021-02-05 2022-08-16 의료법인 성광의료재단 다능성 줄기세포 유래 조혈 내피세포를 이용한 유전자 편집된 세포의 제조방법
GB202102297D0 (en) * 2021-02-18 2021-04-07 Adaptimmune Ltd Methods of Producing Haemogenic Endothelial Cells from Pluripotent Stem Cells
CN113046318B (zh) * 2021-04-13 2023-04-18 中国科学院深圳先进技术研究院 一种诱导多能干细胞向造血前体细胞分化的培养基以及方法
CN117836405A (zh) * 2021-08-13 2024-04-05 先声创新公司 三维培养多能干细胞产生造血干细胞
WO2023201361A1 (en) 2022-04-15 2023-10-19 Aspen Neuroscience, Inc. Methods of classifying the differentiation state of cells and related compositions of differentiated cells

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ553241A (en) 2004-09-08 2009-11-27 Wisconsin Alumni Res Found Medium and culture of pluripotent stem cells
US8034613B2 (en) 2005-06-01 2011-10-11 Wisconsin Alumni Research Foundation Multipotent lymphohematopoietic progenitor cells
CA2610243C (en) 2005-06-01 2016-11-15 Wisconsin Alumni Research Foundation Method of forming dendritic cells from embryonic stem cells
GB2449042B (en) 2006-02-09 2010-07-07 Wisconsin Alumni Res Found Erythroid cells producing adult-type b-hemoglobin generated from human embryonic stem cells
AU2008231020B2 (en) 2007-03-23 2013-09-05 Wisconsin Alumni Research Foundation Somatic cell reprogramming
US20110236971A2 (en) 2007-09-25 2011-09-29 Maksym Vodyanyk Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions
US7615374B2 (en) 2007-09-25 2009-11-10 Wisconsin Alumni Research Foundation Generation of clonal mesenchymal progenitors and mesenchymal stem cell lines under serum-free conditions
WO2009072990A1 (en) 2007-12-06 2009-06-11 Agency For Science, Technology And Research Method for extracellular matrix mediated differentiation and proliferation of stem cells
MX2010012089A (es) * 2008-05-06 2011-07-28 Advanced Cell Tech Inc Metodos para producir celulas eritroides enucleadas derivadas de celulas troncales pluripotentes.
US8372642B2 (en) 2009-02-27 2013-02-12 Cellular Dynamics International, Inc. Differentiation of pluripotent cells
WO2010143529A1 (ja) * 2009-06-09 2010-12-16 国立大学法人名古屋大学 多能性幹細胞を胸腺上皮へ分化誘導する方法
JP2013524836A (ja) * 2010-04-25 2013-06-20 マウント・シナイ・スクール・オブ・メディスン 多能性細胞からの前部前腸内胚葉の生成
US9279107B2 (en) 2010-08-05 2016-03-08 Wisconsin Alumni Research Foundation Simplified basic media for human pluripotent cell culture

Also Published As

Publication number Publication date
EP2970912A1 (en) 2016-01-20
CN105209609A (zh) 2015-12-30
DK2970912T3 (en) 2019-04-23
US20220228118A1 (en) 2022-07-21
PT2970912T (pt) 2019-05-31
HK1218927A1 (zh) 2017-03-17
MX366900B (es) 2019-07-30
AU2014248651A1 (en) 2015-10-01
TR201904270T4 (tr) 2019-05-21
EP2970912B1 (en) 2019-03-06
CN105209609B (zh) 2020-08-21
MX2015012202A (es) 2015-11-30
CA2905786A1 (en) 2014-10-09
WO2014165131A1 (en) 2014-10-09
HUE043785T2 (hu) 2019-09-30
KR102151210B1 (ko) 2020-09-02
CA2905786C (en) 2022-01-25
IL241286B (en) 2020-03-31
IL241286A0 (en) 2015-11-30
KR20150126943A (ko) 2015-11-13
US20140273211A1 (en) 2014-09-18
US20180258398A1 (en) 2018-09-13
AU2014248651B2 (en) 2019-01-17
PL2970912T3 (pl) 2019-08-30
US9938499B2 (en) 2018-04-10
BR112015022770A2 (pt) 2017-07-18
SG11201507275TA (en) 2015-10-29
JP2016512031A (ja) 2016-04-25
ES2720196T3 (es) 2019-07-18
JP6677631B2 (ja) 2020-04-08
CA3141731A1 (en) 2014-10-09
BR112015022770B1 (pt) 2022-11-16

Similar Documents

Publication Publication Date Title
BR112015022770A8 (pt) meio e sistema de cultivo da célula livre de xenógeno, livre de albumina, forma concentrada, métodos de diferenciação de células-tronco pluripotentes humanas, para a produção de mesenquimoangioblastos e sistema definido de cultivo da célula para diferenciação hematoendotelial das células- tronco pluripotentes humanas
WO2014153069A3 (en) Compositions and methods for reprogramming hematopoietic stem cell lineages
BR112015001620A2 (pt) composição de meio de cultura, e método para cultivo de célula ou tecido usando-se dita composição
BR112015030918A2 (pt) método para produção de células progenitoras renais, e fármacos contendo células progenitoras renais
MX2022003477A (es) Medio de cultivo celular complementado con taurina y metodos de uso.
BR112015030946A8 (pt) composição compreendendo uma célula ß pancreática não-nativa, método de diferenciação de células progenitoras em células ß pancreáticas in vitro e uso de uma célula ß pancreática não-nativa
SG10201806217UA (en) Culture medium composition
BR112017007770A2 (pt) cultura in vitro, população de hipscs, método para modificar um locus-alvo genômico, e, hipsc.
EP3546570A4 (en) KIT WITH MEDIUM FOR THE CULTURE OF SERUM-FREE IMMUNELL CELLS, METHOD FOR THE CULTURE OF IMMUNAL CELLS BY USE OF THE KIT, SERUM-FREE IMMUNE CELL CULTURE RECEIVED BY THE KIT OR CULTURE METHOD AND COSMETIC COMPOSITION
WO2016162747A3 (en) Methods and compositions for producing stem cell derived dopaminergic cells for use in treatment of neurodegenerative diseases
WO2012135621A3 (en) Priming of pluripotent stem cells for neural differentiation
CA2909230C (en) Method for inducing alveolar epithelial progenitor cells
WO2014100779A8 (en) Methods for production of platelets from pluripotent stem cells and compositions thereof
EA201391313A1 (ru) C4-монометилтритерпеноидные производные и способы их применения
WO2015065524A3 (en) Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells
WO2014093505A3 (en) Acp-mediated production of fatty acid derivatives
CR20180503A (es) Composiciones y métodos para programar células terapéuticas utilizando nanoportadores de ácidos nucleicos dirigidos
WO2016168890A8 (en) Generation of muscle-lineage cells from stem cells
BR112015021189A2 (pt) métodos de banco de células de alta densidade
CA2775299C (en) Thrombin isolated from blood and blood fractions
MX2018011162A (es) Metodos de purificacion de colageno 7.
EP2582792A4 (en) HEART OF CARDIOMYOCYTES WITH DIALYSATED SERUM
WO2015056258A3 (en) Method of obtaining terminally differentiated neuronal lineages and uses thereof
BR112015009229A2 (pt) composição tendo como alvo a expressão de genes para plaquetas, célula tronco hematopoiética, uso da referida célula tronco e método in vitro ou ex vivo para gerar uma célula tronco modificada
WO2015073625A3 (en) Synergistic genome-nonintegrating reprogramming by micrornas and transcription factors

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 12/03/2014, OBSERVADAS AS CONDICOES LEGAIS