BR112015022770A2 - métodos e materiais para diferenciação hematoendotelial de células-tronco pluripotentes humanas sob condições definidas - Google Patents

métodos e materiais para diferenciação hematoendotelial de células-tronco pluripotentes humanas sob condições definidas

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BR112015022770A2
BR112015022770A2 BR112015022770A BR112015022770A BR112015022770A2 BR 112015022770 A2 BR112015022770 A2 BR 112015022770A2 BR 112015022770 A BR112015022770 A BR 112015022770A BR 112015022770 A BR112015022770 A BR 112015022770A BR 112015022770 A2 BR112015022770 A2 BR 112015022770A2
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stem cells
pluripotent stem
methods
human pluripotent
materials
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BR112015022770A
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BR112015022770A8 (pt
BR112015022770B1 (pt
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Ichiro Uenishi Gene
Slukvin Igor
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Wisconsin Alumni Res Found
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Abstract

abstract methods and compositions for differentiating pluripotent stem cells into cells of endothelial and hematopoietic lineages are disclosed. tradução do resumo resumo patente de invenção: "métodos e materiais para diferenciação hematoendotelial de células-tronco pluripotentes humanas sob condições definidas". o presente pedido de patente refere-se a métodos e composições para a diferenciação de células tronco pluripotentes para células de linhagens hematopoiéticas e endoteliais são divulgados. particularmente, refere-se a um método de diferenciação de células tronco pluripotentes humanas, compreendendo as etapas de (a) forncecer células tronco pluripotentes humanas; e (b) expor as células tronco a uma mistura compreendendo componentes fgf2, bmp4, activina a, e licl, sob condições hipóxicas durante um período de cerca de dois dias de modo a formar uma população de células da mesoderme primitivas emhlin- kdr+aplnr+pdgfralpha+ com potencial mesenquimoangioblasto.
BR112015022770-8A 2013-03-13 2014-03-12 Meio e sistema de cultivo da célula livre de xenógeno, livre de albumina, forma concentrada, métodos de diferenciação de células-tronco pluripotentes humanas, para a produção de mesenquimoangioblastos e sistema definido de cultivo da célula para diferenciação hematoendotelial das células- tronco pluripotentes humanas BR112015022770B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361779564P 2013-03-13 2013-03-13
US61/779,564 2013-03-13
PCT/US2014/024518 WO2014165131A1 (en) 2013-03-13 2014-03-12 Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions

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BR112015022770A2 true BR112015022770A2 (pt) 2017-07-18
BR112015022770A8 BR112015022770A8 (pt) 2019-11-26
BR112015022770B1 BR112015022770B1 (pt) 2022-11-16

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BR112015022770-8A BR112015022770B1 (pt) 2013-03-13 2014-03-12 Meio e sistema de cultivo da célula livre de xenógeno, livre de albumina, forma concentrada, métodos de diferenciação de células-tronco pluripotentes humanas, para a produção de mesenquimoangioblastos e sistema definido de cultivo da célula para diferenciação hematoendotelial das células- tronco pluripotentes humanas

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US (3) US9938499B2 (pt)
EP (1) EP2970912B1 (pt)
JP (1) JP6677631B2 (pt)
KR (1) KR102151210B1 (pt)
CN (1) CN105209609B (pt)
AU (1) AU2014248651B2 (pt)
BR (1) BR112015022770B1 (pt)
CA (2) CA2905786C (pt)
DK (1) DK2970912T3 (pt)
ES (1) ES2720196T3 (pt)
HK (1) HK1218927A1 (pt)
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IL (1) IL241286B (pt)
MX (1) MX366900B (pt)
PL (1) PL2970912T3 (pt)
PT (1) PT2970912T (pt)
SG (1) SG11201507275TA (pt)
TR (1) TR201904270T4 (pt)
WO (1) WO2014165131A1 (pt)

Families Citing this family (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2377924A1 (en) 2006-04-14 2011-10-19 Advanced Cell Technology, Inc. Hemangio-colony forming cells
US9719068B2 (en) 2010-05-06 2017-08-01 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
US9868939B2 (en) 2013-06-12 2018-01-16 Wisconsin Alumni Research Foundation Generating vasculogenic cell populations
US10174289B2 (en) 2014-05-28 2019-01-08 Children's Hospital Medical Center Methods and systems for converting precursor cells into gastric tissues through directed differentiation
WO2016061464A1 (en) 2014-10-17 2016-04-21 Children's Hospital Center, D/B/A Cincinnati Children's Hospital Medical Center In vivo model of human small intetine using pluripotent stem cells and methods of making and using same
US11220672B2 (en) * 2014-12-31 2022-01-11 Wisconsin Alumni Research Foundation Human pluripotent stem cell-based system for generating endothelial cells
US10626372B1 (en) * 2015-01-26 2020-04-21 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
BR112017017886B1 (pt) 2015-02-20 2024-02-06 Wisconsin Alumni Research Foundation Método para obter células endoteliais arteriais humanas, populações isoladas puras de células endoteliais arteriais, método de rastreio de um agente in vitro, método in vitro para vascularizar um construto de tecido engenheirado, uso das referidas células e composição farmacêutica compreendendo as referidas células
US10787640B2 (en) 2015-03-03 2020-09-29 The Board Of Trustees Of The Leland Stanford Junior University Producing mesodermal cell types and methods of using the same
CN104660703B (zh) * 2015-03-05 2017-12-15 国家电网公司 多网络融合用电信息采集系统及最优通信网络的选择方法
JP6773268B2 (ja) * 2015-03-09 2020-10-21 学校法人慶應義塾 多能性幹細胞を所望の細胞型へ分化する方法
EP3313434A4 (en) * 2015-06-29 2019-03-27 Northeastern University GENERATOR OF DENDRITIC CELLS
JP6646311B2 (ja) * 2015-07-17 2020-02-14 国立大学法人京都大学 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法
WO2017070337A1 (en) * 2015-10-20 2017-04-27 Cellular Dynamics International, Inc. Methods for directed differentiation of pluripotent stem cells to immune cells
US10858628B2 (en) 2015-11-04 2020-12-08 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
CN108368520B (zh) 2015-11-04 2023-01-17 菲特治疗公司 多能细胞的基因组工程改造
CN108884441B (zh) * 2016-03-16 2022-05-06 洋蓟治疗有限公司 集落形成培养基及其用途
WO2017181168A1 (en) * 2016-04-15 2017-10-19 Angiocrine Bioscience, Inc. Enhanced gene delivery methods
US11066650B2 (en) 2016-05-05 2021-07-20 Children's Hospital Medical Center Methods for the in vitro manufacture of gastric fundus tissue and compositions related to same
WO2017200486A1 (en) * 2016-05-17 2017-11-23 Agency For Science, Technology And Research Human stem cell derived endothelial cells, endothelial- hepatocyte co-culture system and uses thereof
WO2018037091A1 (en) * 2016-08-24 2018-03-01 Hi-Stem Ggmbh Methods for the identification and isolation of hematopoietic stem and progenitor cells
EP3510145A4 (en) 2016-09-06 2020-03-25 The Children's Medical Center Corporation IMMUNCELLS FROM INDUCED PLURIPOTENT STEM CELLS
CN110050061A (zh) 2016-10-05 2019-07-23 富士胶片细胞动力公司 从具有MeCP2破坏的诱导多能干细胞生成成熟谱系
JP7212615B2 (ja) * 2016-10-05 2023-01-25 フジフィルム セルラー ダイナミクス，インコーポレイテッド 多能性幹細胞からｈｌａホモ接合免疫細胞への指向分化方法
AU2017373767B2 (en) 2016-12-05 2021-09-16 Children's Hospital Medical Center Colonic organoids and methods of making and using same
EP3601540A1 (en) * 2017-03-21 2020-02-05 Etablissement Français du Sang Methods of improving hematopoietic grafts
CN106867961A (zh) * 2017-03-27 2017-06-20 广州润虹医药科技有限公司 一种诱导多能干细胞形成中胚层细胞的诱导培养基及诱导方法
EP3606540A1 (en) * 2017-04-07 2020-02-12 Cynata Therapeutics Limited Method for treating a side effect of chimeric antigen receptor (car) t cell therapy
WO2018227244A1 (en) * 2017-06-16 2018-12-20 Cynata Therapeutics Limited Method for treating a side effect of immunotherapy
WO2018229251A1 (en) * 2017-06-16 2018-12-20 Imba - Institut Für Molekulare Biotechnologie Gmbh Blood vessel organoid, methods of producing and using said organoids
EP3679124A1 (en) * 2017-09-07 2020-07-15 Stemonix Inc. Efficient differentiation of human stem cells to definitive endoderm
US11471493B2 (en) 2017-09-15 2022-10-18 Cynata Therapeutics Limited Method for treating allergic airways disease (AAD)/asthma
CA3084575A1 (en) 2017-11-22 2019-05-31 Mesoblast International Sarl Cellular compositions and methods of treatment i
WO2019126146A1 (en) 2017-12-19 2019-06-27 Xcell Biosciences, Inc. Methods of modulating cell phenotype by way of regulating the gaseous environment
BR112020013656A2 (pt) * 2018-01-05 2020-12-01 Platelet Biogenesis, Inc. composições e métodos para produção de megacariócitos
EP3847243A1 (en) * 2018-09-07 2021-07-14 Wisconsin Alumni Research Foundation Generation of hematopoietic progenitor cells from human pluripotent stem cells
CN109334379B (zh) * 2018-09-19 2021-11-23 江苏大学 重型车用液电式惯容器非线性惯性力辨识方法
US10647954B1 (en) 2018-11-15 2020-05-12 Flaskworks, Llc Dendritic cell generating apparatus and method
WO2020154412A1 (en) * 2019-01-22 2020-07-30 Washington University Compositions and methods for generating hematopoietic stem cells (hscs)
CN109652369B (zh) * 2019-02-28 2020-01-03 西湖生物医药科技(杭州)有限公司 利用外周血体外制备成熟红细胞的方法及制剂
WO2021024207A1 (en) 2019-08-05 2021-02-11 Mesoblast International Sarl Cellular compositions comprising viral vectors and methods of treatment
US11566217B2 (en) 2019-08-13 2023-01-31 Flaskworks, Llc Duty cycle for cell culture systems
US20220275335A1 (en) * 2019-08-20 2022-09-01 Adaptimmune Limited Culture medium for haematopoietic induction
GB201911953D0 (en) * 2019-08-20 2019-10-02 Adaptimmune Ltd T cell production fram RAG inacivated iPSCs
GB201911957D0 (en) * 2019-08-20 2019-10-02 Adaptimmune Ltd Methods of producing haemogenic progenitor cells from pluripotent stem cells
WO2021080847A1 (en) 2019-10-21 2021-04-29 Flaskworks, Llc Systems and methods for cell culturing
JPWO2021085462A1 (pt) * 2019-10-29 2021-05-06
WO2021126841A1 (en) * 2019-12-16 2021-06-24 The Board Of Trustees Of The Leland Stanford Junior University Use of polyvinyl alcohol for cell culture of immune cells
JP7072756B2 (ja) * 2019-12-27 2022-05-23 国立大学法人京都大学 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法
CN113493767B (zh) * 2020-04-01 2024-04-26 北京大学 利用人多潜能干细胞体外制备嗜酸性粒细胞
CN115996735A (zh) 2020-08-10 2023-04-21 迈索布拉斯特国际有限公司 细胞组合物和治疗方法
CA3190402A1 (en) * 2020-08-25 2022-03-03 Gordon Keller Compositions and methods for generating human yolk sac-like hematopoietic cells
US11591381B2 (en) 2020-11-30 2023-02-28 Crispr Therapeutics Ag Gene-edited natural killer cells
WO2022144632A1 (en) 2020-12-30 2022-07-07 Crispr Therapeutics Ag Compositions and methods for differentiating stem cells into nk cells
CN112680410B (zh) * 2021-01-19 2022-08-02 上海爱萨尔生物科技有限公司 诱导多能干细胞分化培养心脏成纤维细胞的方法及其培养液
KR20220113586A (ko) * 2021-02-05 2022-08-16 의료법인 성광의료재단 다능성 줄기세포 유래 조혈 내피세포를 이용한 유전자 편집된 세포의 제조방법
KR20220113290A (ko) * 2021-02-05 2022-08-12 의료법인 성광의료재단 다능성 줄기세포 유래 조혈 내피세포로부터 림프구계세포의 분화 방법
GB202102297D0 (en) * 2021-02-18 2021-04-07 Adaptimmune Ltd Methods of Producing Haemogenic Endothelial Cells from Pluripotent Stem Cells
CN113046318B (zh) * 2021-04-13 2023-04-18 中国科学院深圳先进技术研究院 一种诱导多能干细胞向造血前体细胞分化的培养基以及方法
WO2023019213A1 (en) * 2021-08-13 2023-02-16 Simcere Innovation, Inc. Three-dimensional culturing of pluripotent stem cells to produce hematopoietic stem cells
WO2023201361A1 (en) 2022-04-15 2023-10-19 Aspen Neuroscience, Inc. Methods of classifying the differentiation state of cells and related compositions of differentiated cells

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006029197A1 (en) 2004-09-08 2006-03-16 Wisconsin Alumni Research Foundation Medium and culture of embryonic stem cells
WO2006130651A2 (en) 2005-06-01 2006-12-07 Wisconsin Alumni Research Foundation Method of forming dendritic cells from embryonic stem cells
US8034613B2 (en) 2005-06-01 2011-10-11 Wisconsin Alumni Research Foundation Multipotent lymphohematopoietic progenitor cells
GB2449042B (en) 2006-02-09 2010-07-07 Wisconsin Alumni Res Found Erythroid cells producing adult-type b-hemoglobin generated from human embryonic stem cells
US8440461B2 (en) 2007-03-23 2013-05-14 Wisconsin Alumni Research Foundation Reprogramming somatic cells using retroviral vectors comprising Oct-4 and Sox2 genes
US20110236971A2 (en) 2007-09-25 2011-09-29 Maksym Vodyanyk Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions
US7615374B2 (en) 2007-09-25 2009-11-10 Wisconsin Alumni Research Foundation Generation of clonal mesenchymal progenitors and mesenchymal stem cell lines under serum-free conditions
EP2229435B1 (en) 2007-12-06 2016-03-02 Agency for Science, Technology And Research Method for extracellular matrix mediated differentiation and proliferation of stem cells
EP3282010A1 (en) * 2008-05-06 2018-02-14 Astellas Institute for Regenerative Medicine Methods for producing enucleated erythroid cells derived from pluripotent stem cells
WO2010099539A1 (en) * 2009-02-27 2010-09-02 Cellular Dynamics International, Inc. Differentiation of pluripotent cells
WO2010143529A1 (ja) * 2009-06-09 2010-12-16 国立大学法人名古屋大学 多能性幹細胞を胸腺上皮へ分化誘導する方法
EP2563908B1 (en) * 2010-04-25 2019-01-09 Icahn School of Medicine at Mount Sinai Generation of anterior foregut endoderm from pluripotent cells
US9279107B2 (en) 2010-08-05 2016-03-08 Wisconsin Alumni Research Foundation Simplified basic media for human pluripotent cell culture

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US20140273211A1 (en) 2014-09-18
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