PL2970912T3 - Sposoby i materiały do hematopoetyczno-śródbłonkowego różnicowania ludzkich pluripotentnych komórek macierzystych w zdefiniowanych warunkach - Google Patents

Sposoby i materiały do hematopoetyczno-śródbłonkowego różnicowania ludzkich pluripotentnych komórek macierzystych w zdefiniowanych warunkach

Info

Publication number
PL2970912T3
PL2970912T3 PL14722013T PL14722013T PL2970912T3 PL 2970912 T3 PL2970912 T3 PL 2970912T3 PL 14722013 T PL14722013 T PL 14722013T PL 14722013 T PL14722013 T PL 14722013T PL 2970912 T3 PL2970912 T3 PL 2970912T3
Authority
PL
Poland
Prior art keywords
materials
methods
stem cells
pluripotent stem
cells under
Prior art date
Application number
PL14722013T
Other languages
English (en)
Inventor
Igor Slukvin
Gene Ichiro Uenishi
Original Assignee
Wisconsin Alumni Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Research Foundation filed Critical Wisconsin Alumni Research Foundation
Publication of PL2970912T3 publication Critical patent/PL2970912T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0056—Xeno-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0695—Stem cells; Progenitor cells; Precursor cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/165—Vascular endothelial growth factor [VEGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1394—Bone marrow stromal cells; whole marrow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
PL14722013T 2013-03-13 2014-03-12 Sposoby i materiały do hematopoetyczno-śródbłonkowego różnicowania ludzkich pluripotentnych komórek macierzystych w zdefiniowanych warunkach PL2970912T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361779564P 2013-03-13 2013-03-13
PCT/US2014/024518 WO2014165131A1 (en) 2013-03-13 2014-03-12 Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions
EP14722013.1A EP2970912B1 (en) 2013-03-13 2014-03-12 Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions

Publications (1)

Publication Number Publication Date
PL2970912T3 true PL2970912T3 (pl) 2019-08-30

Family

ID=50678273

Family Applications (1)

Application Number Title Priority Date Filing Date
PL14722013T PL2970912T3 (pl) 2013-03-13 2014-03-12 Sposoby i materiały do hematopoetyczno-śródbłonkowego różnicowania ludzkich pluripotentnych komórek macierzystych w zdefiniowanych warunkach

Country Status (19)

Country Link
US (3) US9938499B2 (pl)
EP (1) EP2970912B1 (pl)
JP (1) JP6677631B2 (pl)
KR (1) KR102151210B1 (pl)
CN (1) CN105209609B (pl)
AU (1) AU2014248651B2 (pl)
BR (1) BR112015022770B1 (pl)
CA (2) CA3141731A1 (pl)
DK (1) DK2970912T3 (pl)
ES (1) ES2720196T3 (pl)
HK (1) HK1218927A1 (pl)
HU (1) HUE043785T2 (pl)
IL (1) IL241286B (pl)
MX (1) MX366900B (pl)
PL (1) PL2970912T3 (pl)
PT (1) PT2970912T (pl)
SG (1) SG11201507275TA (pl)
TR (1) TR201904270T4 (pl)
WO (1) WO2014165131A1 (pl)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5630781B2 (ja) 2006-04-14 2014-11-26 アドバンスド セル テクノロジー、インコーポレイテッド 血管コロニー形成細胞
WO2011140441A2 (en) 2010-05-06 2011-11-10 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
US9868939B2 (en) 2013-06-12 2018-01-16 Wisconsin Alumni Research Foundation Generating vasculogenic cell populations
SG10201801654RA (en) 2014-05-28 2018-04-27 Childrens Hospital Med Ct Methods and systems for converting precursor cells into gastric tissues through directed differentiation
EP3207123A1 (en) 2014-10-17 2017-08-23 Children's Hospital Center D/b/a Cincinnati Children's Hospital Medical Center In vivo model of human small intestine using pluripotent stem cells and methods of making and using same
US11220672B2 (en) * 2014-12-31 2022-01-11 Wisconsin Alumni Research Foundation Human pluripotent stem cell-based system for generating endothelial cells
SG11201706041XA (en) 2015-01-26 2017-08-30 Fate Therapeutics Inc Methods and compositions for inducing hematopoietic cell differentiation
US11674123B2 (en) 2015-02-20 2023-06-13 Wisconsin Alumni Research Foundation Generating arterial endothelial cell populations
US10787640B2 (en) 2015-03-03 2020-09-29 The Board Of Trustees Of The Leland Stanford Junior University Producing mesodermal cell types and methods of using the same
CN104660703B (zh) * 2015-03-05 2017-12-15 国家电网公司 多网络融合用电信息采集系统及最优通信网络的选择方法
WO2016143826A1 (ja) * 2015-03-09 2016-09-15 学校法人慶應義塾 多能性幹細胞を所望の細胞型へ分化する方法
WO2017004169A1 (en) * 2015-06-29 2017-01-05 Northeastern University Dendritic cell generator
JP6646311B2 (ja) * 2015-07-17 2020-02-14 国立大学法人京都大学 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法
CA3002156A1 (en) * 2015-10-20 2017-04-27 FUJIFILM Cellular Dynamics, Inc. Multi-lineage hematopoietic precursor cell production by genetic programming
US10858628B2 (en) 2015-11-04 2020-12-08 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
AU2016349504B2 (en) 2015-11-04 2023-02-09 Fate Therapeutics, Inc. Genomic engineering of pluripotent cells
SG11201807114TA (en) * 2016-03-16 2018-09-27 Cynata Therapeutics Ltd Colony forming medium and use thereof
WO2017181168A1 (en) * 2016-04-15 2017-10-19 Angiocrine Bioscience, Inc. Enhanced gene delivery methods
WO2017192997A1 (en) 2016-05-05 2017-11-09 Children's Hospital Medical Center Methods for the in vitro manufacture of gastric fundus tissue and compositions related to same
CN109153970A (zh) * 2016-05-17 2019-01-04 新加坡科技研究局 人类干细胞衍生的内皮细胞、内皮-肝细胞共培养系统以及其用途
WO2018037091A1 (en) * 2016-08-24 2018-03-01 Hi-Stem Ggmbh Methods for the identification and isolation of hematopoietic stem and progenitor cells
WO2018048828A1 (en) 2016-09-06 2018-03-15 The Children's Medical Center Corporation Immune cells derived from induced pluripotent stem cell
US20200263139A1 (en) * 2016-10-05 2020-08-20 FUJIFILM Cellular Dynamics, Inc. Methods for directed differentiation of pluripotent stem cells to hla homozygous immune cells
WO2018067826A1 (en) 2016-10-05 2018-04-12 Cellular Dynamics International, Inc. Generating mature lineages from induced pluripotent stem cells with mecp2 disruption
SG10202105768WA (en) 2016-12-05 2021-06-29 Childrens Hospital Med Ct Colonic organoids and methods of making and using same
CA3056709A1 (en) * 2017-03-21 2018-09-27 Etablissement Francais Du Sang Methods of improving hematopoietic grafts
CN106867961A (zh) * 2017-03-27 2017-06-20 广州润虹医药科技有限公司 一种诱导多能干细胞形成中胚层细胞的诱导培养基及诱导方法
RU2019133327A (ru) * 2017-04-07 2021-05-07 Сината Терапьютикс Лимитед Способ лечения побочного эффекта терапии Т-клетками с химерными антигенными рецепторами (CAR)
US20200199541A1 (en) * 2017-06-16 2020-06-25 Imba - Institut Für Molekulare Biotechnologie Gmbh Blood vessel organoid, methods of producing and using said organoids
EP3638264A1 (en) * 2017-06-16 2020-04-22 Cynata Therapeutics Limited Method for treating a side effect of immunotherapy
WO2019051281A1 (en) * 2017-09-07 2019-03-14 StemoniX Inc. EFFECTIVE DIFFERENTIATION OF HUMAN STEM CELLS IN DEFINITIVE ENDODERM
CN111093680A (zh) 2017-09-15 2020-05-01 洋蓟治疗有限公司 治疗变应性气道疾病(aad)/哮喘的方法
CN112004923A (zh) 2017-11-22 2020-11-27 迈索布拉斯特国际有限公司 细胞组合物及治疗方法
US11365390B2 (en) 2017-12-19 2022-06-21 Xcell Biosciences, Inc. Methods of modulating cell phenotype by way of regulating the gaseous environment
EP3735457A4 (en) * 2018-01-05 2021-08-25 Platelet Biogenesis, Inc. COMPOSITIONS AND METHOD OF MANUFACTURING MEGAKARYOCYTE
WO2020051453A1 (en) * 2018-09-07 2020-03-12 Wisconsin Alumni Research Foundation Generation of hematopoietic progenitor cells from human pluripotent stem cells
CN109334379B (zh) * 2018-09-19 2021-11-23 江苏大学 重型车用液电式惯容器非线性惯性力辨识方法
US10647954B1 (en) 2018-11-15 2020-05-12 Flaskworks, Llc Dendritic cell generating apparatus and method
US20220025330A1 (en) * 2019-01-22 2022-01-27 Washington University Compositions and methods for generating hematopoietic stem cells (hscs)
CN109652369B (zh) * 2019-02-28 2020-01-03 西湖生物医药科技(杭州)有限公司 利用外周血体外制备成熟红细胞的方法及制剂
US20220275337A1 (en) 2019-08-05 2022-09-01 Mesoblast International Sárl Cellular compositions comprising viral vectors and methods of treatment
US11566217B2 (en) 2019-08-13 2023-01-31 Flaskworks, Llc Duty cycle for cell culture systems
GB201911953D0 (en) * 2019-08-20 2019-10-02 Adaptimmune Ltd T cell production fram RAG inacivated iPSCs
WO2021032855A1 (en) * 2019-08-20 2021-02-25 Adaptimmune Limited Culture medium for haematopoietic induction
GB201911957D0 (en) * 2019-08-20 2019-10-02 Adaptimmune Ltd Methods of producing haemogenic progenitor cells from pluripotent stem cells
KR20220086625A (ko) 2019-10-21 2022-06-23 플라스크웍스, 엘엘씨 세포 배양을 위한 시스템 및 방법
WO2021085462A1 (ja) * 2019-10-29 2021-05-06 国立大学法人京都大学 多能性幹細胞から造血性内皮細胞および／または造血前駆細胞を製造する方法
WO2021126841A1 (en) * 2019-12-16 2021-06-24 The Board Of Trustees Of The Leland Stanford Junior University Use of polyvinyl alcohol for cell culture of immune cells
JP7072756B2 (ja) * 2019-12-27 2022-05-23 国立大学法人京都大学 多能性幹細胞から中胚葉前駆細胞および血液血管前駆細胞への分化誘導法
WO2022034506A1 (en) 2020-08-10 2022-02-17 Mesoblast International Sárl Cellular compositions and methods of treatment
EP4204544A1 (en) * 2020-08-25 2023-07-05 University Health Network Compositions and methods for generating human yolk sac-like hematopoietic cells
US11459372B2 (en) 2020-11-30 2022-10-04 Crispr Therapeutics Ag Gene-edited natural killer cells
EP4271798A1 (en) 2020-12-30 2023-11-08 CRISPR Therapeutics AG Compositions and methods for differentiating stem cells into nk cells
CN112680410B (zh) * 2021-01-19 2022-08-02 上海爱萨尔生物科技有限公司 诱导多能干细胞分化培养心脏成纤维细胞的方法及其培养液
EP4293107A1 (en) * 2021-02-05 2023-12-20 Sungkwang Medical Foundation Method for differentiating pluripotent stem cell-derived hemogenic endothelial cells into lymphoid lineage cells
KR20220113586A (ko) * 2021-02-05 2022-08-16 의료법인 성광의료재단 다능성 줄기세포 유래 조혈 내피세포를 이용한 유전자 편집된 세포의 제조방법
GB202102297D0 (en) * 2021-02-18 2021-04-07 Adaptimmune Ltd Methods of Producing Haemogenic Endothelial Cells from Pluripotent Stem Cells
CN113046318B (zh) * 2021-04-13 2023-04-18 中国科学院深圳先进技术研究院 一种诱导多能干细胞向造血前体细胞分化的培养基以及方法
WO2023019213A1 (en) * 2021-08-13 2023-02-16 Simcere Innovation, Inc. Three-dimensional culturing of pluripotent stem cells to produce hematopoietic stem cells
US20230377685A1 (en) 2022-04-15 2023-11-23 Aspen Neuroscience, Inc. Methods of classifying the differentiation state of cells and related compositions of differentiated cells

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005282510B2 (en) 2004-09-08 2010-12-02 Wisconsin Alumni Research Foundation Medium and culture of embryonic stem cells
US8034613B2 (en) 2005-06-01 2011-10-11 Wisconsin Alumni Research Foundation Multipotent lymphohematopoietic progenitor cells
SE531979C2 (sv) 2005-06-01 2009-09-22 Wisconsin Alumni Res Found Metod för att framställa dendritiska celler från mänskliga embryonala stamceller, samt odlingar framställda med metoden
US8158422B2 (en) 2006-02-09 2012-04-17 Wisconsin Alumni Research Foundation Erythroid cells producing adult-type β-hemoglobin generated from human embryonic stem cells
EP2137296A2 (en) 2007-03-23 2009-12-30 Wisconsin Alumni Research Foundation Somatic cell reprogramming
US20110236971A2 (en) * 2007-09-25 2011-09-29 Maksym Vodyanyk Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions
US7615374B2 (en) 2007-09-25 2009-11-10 Wisconsin Alumni Research Foundation Generation of clonal mesenchymal progenitors and mesenchymal stem cell lines under serum-free conditions
EP2229435B1 (en) 2007-12-06 2016-03-02 Agency for Science, Technology And Research Method for extracellular matrix mediated differentiation and proliferation of stem cells
JP5748654B2 (ja) * 2008-05-06 2015-07-15 オカタ セラピューティクス， インコーポレイテッド 多能性幹細胞に由来する除核赤血球細胞を産生するための方法
US8372642B2 (en) 2009-02-27 2013-02-12 Cellular Dynamics International, Inc. Differentiation of pluripotent cells
WO2010143529A1 (ja) * 2009-06-09 2010-12-16 国立大学法人名古屋大学 多能性幹細胞を胸腺上皮へ分化誘導する方法
US9234170B2 (en) * 2010-04-25 2016-01-12 Mount Sinai School Of Medicine Generation of anterior foregut endoderm from pluripotent cells
US9279107B2 (en) 2010-08-05 2016-03-08 Wisconsin Alumni Research Foundation Simplified basic media for human pluripotent cell culture

Also Published As

Publication number Publication date
CA2905786A1 (en) 2014-10-09
US9938499B2 (en) 2018-04-10
WO2014165131A1 (en) 2014-10-09
CN105209609A (zh) 2015-12-30
HK1218927A1 (zh) 2017-03-17
AU2014248651A1 (en) 2015-10-01
KR102151210B1 (ko) 2020-09-02
DK2970912T3 (en) 2019-04-23
IL241286A0 (en) 2015-11-30
JP2016512031A (ja) 2016-04-25
AU2014248651B2 (en) 2019-01-17
CA2905786C (en) 2022-01-25
US20220228118A1 (en) 2022-07-21
JP6677631B2 (ja) 2020-04-08
US20140273211A1 (en) 2014-09-18
MX366900B (es) 2019-07-30
US20180258398A1 (en) 2018-09-13
CA3141731A1 (en) 2014-10-09
CN105209609B (zh) 2020-08-21
SG11201507275TA (en) 2015-10-29
BR112015022770B1 (pt) 2022-11-16
EP2970912A1 (en) 2016-01-20
PT2970912T (pt) 2019-05-31
HUE043785T2 (hu) 2019-09-30
IL241286B (en) 2020-03-31
ES2720196T3 (es) 2019-07-18
BR112015022770A2 (pt) 2017-07-18
TR201904270T4 (tr) 2019-05-21
KR20150126943A (ko) 2015-11-13
BR112015022770A8 (pt) 2019-11-26
EP2970912B1 (en) 2019-03-06
MX2015012202A (es) 2015-11-30

Similar Documents

Publication Publication Date Title
HK1218927A1 (zh) 人類多能幹細胞在限定條件下進行血液內皮分化的方法和材料
IL241930B (en) Isolated naive pluripotent stem cells and methods of generating same
HK1210494A1 (zh) 用於細胞培養的裝置和方法
IL236672B (en) System and methods for preparing vegetable cells from animal fats
HK1216851A1 (zh) 用於提高細胞存活力的組合物和使用該組合物的方法
SG11201603047PA (en) Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells
SG11201507510PA (en) Isolation of non-embryonic stem cells and uses thereof
EP2904103A4 (en) PROCESS FOR THE PREPARATION OF INDUCED PLURIPOTENT STEM CELLS AND APPLICATIONS THEREOF
HK1220487A1 (zh) 全能幹細胞的誘導方法、及用該方法製備的全能幹細胞
EP2949746A4 (en) METHOD OF SUB-CULTURE OF PLURIPOTENT STEM CELLS
GB201515255D0 (en) Devices and methods for purification of biological cells
IL245272A0 (en) pif-transfected cells and methods of use
EP3070159A4 (en) METHOD AND PLANT FOR CURING PLURIPOTENT STEM CELLS
EP3088415A4 (en) Method for inducing differentiation of pluripotent stem cells into endodermal cells
EP3064576A4 (en) POLYPEPTIDE COMPOSITION AND METHOD FOR CULTURING PLURIPOTENT STEM CELLS USING THE SAME
SG11201406771PA (en) Stem cell preparations and methods of use
GB201307486D0 (en) Cell-cycle directed differentiation of pluripotent cells
EP3045531A4 (en) Method for inducing differentiation of induced pluripotent stem cells and method for selecting induced pluripotent stem cells
GB201307377D0 (en) Devices and methods for culture of cells
GB201317657D0 (en) Methods for differentiation of primate pluripotent stem cells
GB201303823D0 (en) Methods for characterising pluripotent cells and prediction of differentiation capacity