EA201490480A1 - Интегрированный способ высокопроизводительной идентификации новых пестицидных композиций и его применение - Google Patents

Интегрированный способ высокопроизводительной идентификации новых пестицидных композиций и его применение

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Publication number
EA201490480A1
EA201490480A1 EA201490480A EA201490480A EA201490480A1 EA 201490480 A1 EA201490480 A1 EA 201490480A1 EA 201490480 A EA201490480 A EA 201490480A EA 201490480 A EA201490480 A EA 201490480A EA 201490480 A1 EA201490480 A1 EA 201490480A1
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EA
Eurasian Patent Office
Prior art keywords
application
methods
high performance
compositions
integrated method
Prior art date
Application number
EA201490480A
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English (en)
Other versions
EA025208B1 (ru
Inventor
Кристофер Дж. Грэндлик
Тоби Ричардсон
Янне С. Керовуо
Ариэль Шварц
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Синтетик Дженомикс, Инк.
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Application filed by Синтетик Дженомикс, Инк. filed Critical Синтетик Дженомикс, Инк.
Publication of EA201490480A1 publication Critical patent/EA201490480A1/ru
Publication of EA025208B1 publication Critical patent/EA025208B1/ru

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Abstract

Предложены способы быстрой идентификации последовательностей нуклеиновой кислоты, которые кодируют новые биотоксины. В частности, описаны способы быстрого отбора и скрининга внехромосомного генетического содержимого микроорганизмов для получения новых представляющих интерес последовательностей. Предложены композиции, которые содержат кодирующие последовательности для биотоксинов и полипептиды, и вытекающие из них применения. Композиции и способы применимы, например, для придания пестицидной активности бактериям, растениям, растительным клеткам, тканям и семенам.
EA201490480A 2011-08-19 2012-08-17 Интегрированный способ высокопроизводительной идентификации новых пестицидных композиций и его применение EA025208B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161525674P 2011-08-19 2011-08-19
PCT/US2012/051466 WO2013028563A2 (en) 2011-08-19 2012-08-17 Integrated method for high-throughput identification of novel pesticidal compositions and uses therefor

Publications (2)

Publication Number Publication Date
EA201490480A1 true EA201490480A1 (ru) 2014-06-30
EA025208B1 EA025208B1 (ru) 2016-11-30

Family

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EA201490480A EA025208B1 (ru) 2011-08-19 2012-08-17 Интегрированный способ высокопроизводительной идентификации новых пестицидных композиций и его применение

Country Status (10)

Country Link
US (1) US20130227743A1 (ru)
EP (1) EP2744920A4 (ru)
JP (1) JP6230125B2 (ru)
CN (1) CN103946393A (ru)
AU (2) AU2012299142A1 (ru)
BR (1) BR112014003911A2 (ru)
CA (1) CA2844913A1 (ru)
EA (1) EA025208B1 (ru)
MX (1) MX2014002027A (ru)
WO (1) WO2013028563A2 (ru)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106133142B (zh) * 2014-03-28 2020-10-30 孟山都技术公司 具有抗鞘翅目昆虫活性的杀虫毒素蛋白
WO2016154602A1 (en) * 2015-03-26 2016-09-29 Epibiome, Inc. Compositions and methods for pest control
US20160345590A1 (en) * 2015-05-01 2016-12-01 Board Of Regents, The University Of Texas System Chlamydomonas Strains With Chloroplast-Expressed Cry Proteins For Biological Control Of Mosquitoes That Transmit Disease
CN108271390A (zh) 2015-06-03 2018-07-10 农业生物群落股份有限公司 杀虫基因和使用方法
CA2989169A1 (en) * 2015-06-22 2016-12-29 AgBiome, Inc. Pesticidal genes and methods of use
RU2765310C2 (ru) * 2015-07-30 2022-01-28 Монсанто Текнолоджи Ллс Новые белки, проявляющие ингибирующую активность по отношению к насекомым
MA42987A (fr) * 2015-10-14 2021-06-02 BASF Agricultural Solutions Seed US LLC Gène axmi554 de l'endotoxine delta et ses procédés d'utilisation
CN109152369A (zh) * 2016-05-17 2019-01-04 先锋国际良种公司 使用针对鞘翅目有害生物的cyt1a突变体的方法
WO2018048915A1 (en) 2016-09-06 2018-03-15 AgBiome, Inc. Pesticidal genes and methods of use
BR112020012763A2 (pt) * 2017-12-22 2020-12-15 AgBiome, Inc. Genes pesticidas e métodos de uso
CN108359673A (zh) * 2018-05-08 2018-08-03 江苏省农业科学院 一种高效杀食用菌眼蕈蚊的Bt cry11基因、编码蛋白及其应用
MX2021004825A (es) * 2018-10-30 2021-06-15 Syngenta Participations Ag Proteinas insecticidas.
WO2022072480A1 (en) * 2020-09-30 2022-04-07 Zymergen Inc. Methods to identify novel insecticidal proteins from complex metagenomic microbial samples
WO2024035660A2 (en) * 2022-08-06 2024-02-15 The Johns Hopkins University Methods, systems and kits for identifying bio active compounds and therapeutic methods and compositions

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2000601A (en) 1931-07-28 1935-05-07 Eastman Kodak Co Precipitation of cellulose esters
US2007727A (en) 1931-09-21 1935-07-09 Earl B Putt Flavoring and coloring extract
US2007724A (en) 1932-09-17 1935-07-09 Palli Stefano Tubular rubber tire with metal springs
US2007728A (en) 1932-11-22 1935-07-09 George Russell Safety toe cap
US2007723A (en) 1933-01-10 1935-07-09 Midd Alphonso Thomas A Douglas Manufacture of fish liver oils
US4196265A (en) 1977-06-15 1980-04-01 The Wistar Institute Method of producing antibodies
US4695462A (en) 1985-06-28 1987-09-22 Mycogen Corporation Cellular encapsulation of biological pesticides
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US5039523A (en) 1988-10-27 1991-08-13 Mycogen Corporation Novel Bacillus thuringiensis isolate denoted B.t. PS81F, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5240842A (en) 1989-07-11 1993-08-31 Biotechnology Research And Development Corporation Aerosol beam microinjector
ATE191931T1 (de) 1990-11-23 2000-05-15 Plant Genetic Systems Nv Verfahren zur transformation monokotyler pflanzen
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5308760A (en) 1992-01-10 1994-05-03 Washington Research Foundation Crystal proteins of Bacillus thuringiensis, genes encoding them, and host expressing them
US5743477A (en) 1992-08-27 1998-04-28 Dowelanco Insecticidal proteins and method for plant protection
US5527883A (en) 1994-05-06 1996-06-18 Mycogen Corporation Delta-endotoxin expression in pseudomonas fluorescens
US6093695A (en) * 1996-09-26 2000-07-25 Monsanto Company Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and ctenocephalides SPP
US6603063B1 (en) 1999-05-07 2003-08-05 Mycogen Corp. Plants and cells transformed with a nucleic acid from Bacillus thuringiensis strain KB59A4-6 encoding a novel SUP toxin
US7129212B2 (en) 1996-10-30 2006-10-31 Mycogen Corporation Polynucleotides, pesticidal proteins, and novel methods of using them
US6468523B1 (en) 1998-11-02 2002-10-22 Monsanto Technology Llc Polypeptide compositions toxic to diabrotic insects, and methods of use
EP1250447B1 (en) 1999-11-29 2011-12-21 Midwest Oilseeds, Inc. Methods, media and apparatus for the introduction of molecules into plant cells and bacteria using aerosol beams
EP3034627B1 (en) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massive parallel method for decoding dna and rna
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AU2003218114B2 (en) * 2002-03-11 2008-09-18 Athenix Corporation Integrated system for high throughput capture of genetic diversity
AU2004208093A1 (en) * 2003-01-21 2004-08-12 Dow Agrosciences Llc Mixing and matching TC proteins for pest control
AU2004223493A1 (en) 2003-02-28 2004-10-07 Brown University Nanopores, methods for using same, methods for making same and methods for characterizing biomolecules using same
WO2005042781A2 (en) 2003-10-31 2005-05-12 Agencourt Personal Genomics Corporation Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof
EP1580273A1 (en) * 2004-03-26 2005-09-28 Friedrich-Alexander-Universität Erlangen-Nürnberg Peptide-based method for monitoring gene expression in a host cell
US20060231419A1 (en) 2005-04-15 2006-10-19 Barth Philip W Molecular resonant tunneling sensor and methods of fabricating and using the same
WO2007145612A1 (en) 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
US20060287833A1 (en) 2005-06-17 2006-12-21 Zohar Yakhini Method and system for sequencing nucleic acid molecules using sequencing by hybridization and comparison with decoration patterns
US20070048745A1 (en) 2005-08-30 2007-03-01 Joyce Timothy H Systems and methods for partitioned nanopore analysis of polymers
EP1920060B1 (en) * 2005-08-31 2011-04-13 Monsanto Technology LLC Insecticidal compositions and methods for making insect-resistant transgenic plants
WO2007041621A2 (en) 2005-10-03 2007-04-12 Xingsheng Sean Ling Hybridization assisted nanopore sequencing
US20070172839A1 (en) 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
US20070178507A1 (en) 2006-01-31 2007-08-02 Wei Wu Method and apparatus for detection of molecules using nanopores
ES2864641T3 (es) * 2006-06-15 2021-10-14 BASF Agricultural Solutions Seed US LLC Una familia de proteínas pesticidas y procedimientos para su uso
CN105002189A (zh) * 2008-06-25 2015-10-28 阿森尼克斯公司 毒素基因及其使用方法
AU2010217915B2 (en) * 2009-02-27 2016-03-17 BASF Agricultural Solutions Seed US LLC Pesticidal proteins and methods for their use
US20100298211A1 (en) * 2009-03-11 2010-11-25 Athenix Corporation Axmi-001, axmi-002, axmi-030, axmi-035, and axmi-045: toxin genes and methods for their use
WO2010115156A2 (en) 2009-04-03 2010-10-07 Synthetic Genomics, Inc. Endophytic fungus and uses therefor
WO2011009182A2 (pt) 2009-07-24 2011-01-27 Embrapa - Empresa Brasileira De Pesquisa Agropecuária Molécula de ácido nucléico isolada, construção gênica, vetor, célula transgênica, método para obtenção de uma célula e de uma planta transgênica, polipeptídeo isolado e purificado, composição pesticida biodegradável, método para o controle de uma praga, método de obtenção de linhagens transgênicas resistentes a um inseto praga

Also Published As

Publication number Publication date
JP6230125B2 (ja) 2017-11-15
CN103946393A (zh) 2014-07-23
CA2844913A1 (en) 2013-02-28
AU2018200012A1 (en) 2018-01-25
WO2013028563A2 (en) 2013-02-28
EP2744920A2 (en) 2014-06-25
WO2013028563A3 (en) 2014-05-15
MX2014002027A (es) 2014-11-10
EP2744920A4 (en) 2015-06-03
BR112014003911A2 (pt) 2017-03-14
JP2014526893A (ja) 2014-10-09
EA025208B1 (ru) 2016-11-30
US20130227743A1 (en) 2013-08-29
AU2018200012A8 (en) 2018-02-22
AU2012299142A1 (en) 2014-02-27

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