MX343604B - Disminuir nivel de lactato e incrementar producción de polipéptidos por regulación a la baja de la expresión de lactato dehidrogenasa y piruvato dehidrogenasa quinasa. - Google Patents

Disminuir nivel de lactato e incrementar producción de polipéptidos por regulación a la baja de la expresión de lactato dehidrogenasa y piruvato dehidrogenasa quinasa.

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Publication number
MX343604B
MX343604B MX2012013579A MX2012013579A MX343604B MX 343604 B MX343604 B MX 343604B MX 2012013579 A MX2012013579 A MX 2012013579A MX 2012013579 A MX2012013579 A MX 2012013579A MX 343604 B MX343604 B MX 343604B
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MX
Mexico
Prior art keywords
ldh
pdhk
downregulating
polypeptide production
increasing polypeptide
Prior art date
Application number
MX2012013579A
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English (en)
Other versions
MX2012013579A (es
Inventor
Meixia Zhou
Bradley Richard Snedecor
Chi Kin Domingos Ng
Amy Shen
Original Assignee
Genentech Inc
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Application filed by Genentech Inc filed Critical Genentech Inc
Publication of MX2012013579A publication Critical patent/MX2012013579A/es
Publication of MX343604B publication Critical patent/MX343604B/es

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Abstract

La presente invención se refiere a métodos y composiciones para reducir producción de lactato e incrementar producción de polipéptido en células cultivadas. En un aspecto, la invención proporciona un método que comprende cultivar células que expresan a) un RNA pequeño interferente (siRNA) específico para una lactato dehidrogenasa (LDH) y b) un siRNA específico para una piruvato dehidrogenasa quinasa (PDHK). En otro aspecto, la invención proporciona células cultivadas o vectores que comprenden un siRNA específico para un LDH y un siRNA específico para un PDHK.
MX2012013579A 2010-05-28 2011-05-26 Disminuir nivel de lactato e incrementar producción de polipéptidos por regulación a la baja de la expresión de lactato dehidrogenasa y piruvato dehidrogenasa quinasa. MX343604B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34972710P 2010-05-28 2010-05-28
PCT/US2011/038191 WO2011150241A2 (en) 2010-05-28 2011-05-26 Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase

Publications (2)

Publication Number Publication Date
MX2012013579A MX2012013579A (es) 2013-01-24
MX343604B true MX343604B (es) 2016-11-11

Family

ID=45004831

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2012013579A MX343604B (es) 2010-05-28 2011-05-26 Disminuir nivel de lactato e incrementar producción de polipéptidos por regulación a la baja de la expresión de lactato dehidrogenasa y piruvato dehidrogenasa quinasa.
MX2020009460A MX2020009460A (es) 2010-05-28 2012-11-22 Disminuir nivel de lactato e incrementar produccion de polipeptidos por regulacion a la baja de la expresion de lactato dehidrogenasa y piruvato dehidrogenasa quinasa.

Family Applications After (1)

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MX2020009460A MX2020009460A (es) 2010-05-28 2012-11-22 Disminuir nivel de lactato e incrementar produccion de polipeptidos por regulacion a la baja de la expresion de lactato dehidrogenasa y piruvato dehidrogenasa quinasa.

Country Status (15)

Country Link
US (4) US9487809B2 (es)
EP (1) EP2576580B1 (es)
JP (3) JP6050226B2 (es)
KR (3) KR101989762B1 (es)
CN (1) CN102985437B (es)
BR (1) BR112012030197A2 (es)
CA (1) CA2800728C (es)
DK (1) DK2576580T3 (es)
ES (1) ES2599412T3 (es)
HU (1) HUE030820T2 (es)
MX (2) MX343604B (es)
PL (1) PL2576580T3 (es)
RU (1) RU2614125C2 (es)
SI (1) SI2576580T1 (es)
WO (1) WO2011150241A2 (es)

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