BR112012030197A2 - diminuição do nível de lactato e aumento da produção de poliptídeo por subregulação da expressão de lactato desidrogenase e piruvato desidrogenase cinase. - Google Patents

diminuição do nível de lactato e aumento da produção de poliptídeo por subregulação da expressão de lactato desidrogenase e piruvato desidrogenase cinase.

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Publication number
BR112012030197A2
BR112012030197A2 BR112012030197A BR112012030197A BR112012030197A2 BR 112012030197 A2 BR112012030197 A2 BR 112012030197A2 BR 112012030197 A BR112012030197 A BR 112012030197A BR 112012030197 A BR112012030197 A BR 112012030197A BR 112012030197 A2 BR112012030197 A2 BR 112012030197A2
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Prior art keywords
lactate
dehydrogenase
polypeptide production
downregulating
decreased
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BR112012030197A
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English (en)
Inventor
Amy Shen
Bradley Richard Snedecor
Chi Kin Domingos Ng
Meixia Zhou
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Genentech Inc
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Application filed by Genentech Inc filed Critical Genentech Inc
Publication of BR112012030197A2 publication Critical patent/BR112012030197A2/pt

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Abstract

diminuição do nível de lactatto e aumento da produção de polipeptídeo por sub-regulação da expressão de lactato desidrogenase e piruvato desidrogenase cinase - a presente invenção refere-se a métodos e composições para reduzir a produção de lactado e aumentar a produção de polipeptídeo em células cultivadas. em um aspecto, a invenção fornece um método compreendendo cultivar as células que expressam a) um rna de interferência pequeno (sirna) específico para uma lactato desidrogenase (ldh) e b) um sirna específico para uma piruvato desidrogenase cinase (pshk). em outro aspecto, a invenção fornece células cultivadas ou vetores que compreendem um sirna específico para uma ldh e um sirna específico para uma pdhk.
BR112012030197A 2010-05-28 2011-05-26 diminuição do nível de lactato e aumento da produção de poliptídeo por subregulação da expressão de lactato desidrogenase e piruvato desidrogenase cinase. BR112012030197A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34972710P 2010-05-28 2010-05-28
PCT/US2011/038191 WO2011150241A2 (en) 2010-05-28 2011-05-26 Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase

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BR112012030197A2 true BR112012030197A2 (pt) 2015-09-29

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BR112012030197A BR112012030197A2 (pt) 2010-05-28 2011-05-26 diminuição do nível de lactato e aumento da produção de poliptídeo por subregulação da expressão de lactato desidrogenase e piruvato desidrogenase cinase.

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US (4) US9487809B2 (pt)
EP (1) EP2576580B1 (pt)
JP (3) JP6050226B2 (pt)
KR (3) KR101869987B1 (pt)
CN (1) CN102985437B (pt)
BR (1) BR112012030197A2 (pt)
CA (1) CA2800728C (pt)
DK (1) DK2576580T3 (pt)
ES (1) ES2599412T3 (pt)
HU (1) HUE030820T2 (pt)
MX (2) MX343604B (pt)
PL (1) PL2576580T3 (pt)
RU (1) RU2614125C2 (pt)
SI (1) SI2576580T1 (pt)
WO (1) WO2011150241A2 (pt)

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