MX352837B - Método para marcaje de objetivos intracelulares y extracelulares de leucocitos. - Google Patents
Método para marcaje de objetivos intracelulares y extracelulares de leucocitos.Info
- Publication number
- MX352837B MX352837B MX2014007284A MX2014007284A MX352837B MX 352837 B MX352837 B MX 352837B MX 2014007284 A MX2014007284 A MX 2014007284A MX 2014007284 A MX2014007284 A MX 2014007284A MX 352837 B MX352837 B MX 352837B
- Authority
- MX
- Mexico
- Prior art keywords
- leukocytes
- extracellular targets
- labeling intracellular
- intracellular
- labeling
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 230000003834 intracellular effect Effects 0.000 title abstract 2
- 238000002372 labelling Methods 0.000 title abstract 2
- 210000000265 leukocyte Anatomy 0.000 title abstract 2
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
- G01N33/56972—White blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
- G01N2333/70514—CD4
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/70589—CD45
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/70596—Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- G01N2333/91205—Phosphotransferases in general
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Virology (AREA)
- Ecology (AREA)
- Physiology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
La presente invención se refiere a métodos para etiquetar objetivos intracelulares y extracelulares de leucocitos y a kits para llevar a cabo dichos métodos.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP11290586.4A EP2607899B1 (en) | 2011-12-21 | 2011-12-21 | Method for labeling intracellular and extracellular targets of leukocytes |
PCT/IB2012/003071 WO2013093642A1 (en) | 2011-12-21 | 2012-12-21 | Method for labeling intracellular and extracellular targets of leukocytes |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2014007284A MX2014007284A (es) | 2015-10-29 |
MX352837B true MX352837B (es) | 2017-12-11 |
Family
ID=47563336
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2014007284A MX352837B (es) | 2011-12-21 | 2012-12-21 | Método para marcaje de objetivos intracelulares y extracelulares de leucocitos. |
Country Status (10)
Country | Link |
---|---|
US (3) | US9678073B2 (es) |
EP (2) | EP3104177B1 (es) |
JP (1) | JP6053818B2 (es) |
KR (1) | KR102067742B1 (es) |
CN (1) | CN104011542B (es) |
AU (2) | AU2012356324A1 (es) |
BR (1) | BR112014015204B1 (es) |
CA (1) | CA2859605C (es) |
MX (1) | MX352837B (es) |
WO (1) | WO2013093642A1 (es) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3104177B1 (en) | 2011-12-21 | 2020-11-25 | Beckman Coulter, Inc. | Method for labeling intracellular and extracellular targets of leukocytes |
CA2936167A1 (en) | 2015-07-15 | 2017-01-15 | Streck, Inc. | Method and device for stabilizing of proteins |
JPWO2017141789A1 (ja) * | 2016-02-19 | 2018-12-13 | コニカミノルタ株式会社 | 血液由来検体の保管方法および希少細胞の判定方法 |
WO2019118355A1 (en) * | 2017-12-12 | 2019-06-20 | 10X Genomics, Inc. | Systems and methods for single cell processing |
SG11202011500UA (en) * | 2018-07-12 | 2020-12-30 | Beckman Coulter Inc | Fixable viability dyes and their uses |
CN111807809B (zh) * | 2019-04-10 | 2021-12-28 | 盐城工业职业技术学院 | 一种棕榈纳米纤维-石墨烯-碳纳米管复合气凝胶的制备方法 |
CN113884671A (zh) * | 2021-09-27 | 2022-01-04 | 苏州东岭生物技术有限公司 | 一种流式染色试剂盒及其配置方法和应用方法 |
Family Cites Families (25)
Publication number | Priority date | Publication date | Assignee | Title |
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JPS6179163A (ja) * | 1984-09-26 | 1986-04-22 | Toa Medical Electronics Co Ltd | 血球測定用試薬 |
JP2891302B2 (ja) * | 1990-03-01 | 1999-05-17 | シスメックス株式会社 | 血液中の白血球およびヘモグロビンの測定用試薬 |
WO1992010395A1 (en) * | 1990-12-08 | 1992-06-25 | Maeda Industries, Ltd. | Change gear mechanism for bicycle |
US5422277A (en) * | 1992-03-27 | 1995-06-06 | Ortho Diagnostic Systems Inc. | Cell fixative composition and method of staining cells without destroying the cell surface |
DE69434942T2 (de) * | 1993-02-25 | 2007-11-29 | Abbott Laboratories, Abbott Park | Mehrzweckreagenzsystem zur schnellen lysierung von vollblutproben |
US5891734A (en) * | 1994-08-01 | 1999-04-06 | Abbott Laboratories | Method for performing automated analysis |
JP3170176B2 (ja) * | 1995-05-17 | 2001-05-28 | 大塚製薬株式会社 | 薬剤耐性獲得癌細胞の検出方法 |
US6319683B1 (en) * | 1996-04-12 | 2001-11-20 | Intergen Company | Method and composition for controlling formaldehyde fixation by delayed quenching |
JP2002523782A (ja) * | 1998-08-27 | 2002-07-30 | マヨ ファウンデーション フォー メディカル エデュケーション アンド リサーチ | 不安定アンギナを診断するための方法および材料 |
US6210969B1 (en) * | 1999-04-28 | 2001-04-03 | Coulter International Corp. | Composition and method for differentiation of basophil and eosinophil subpopulations of leukocytes in blood |
US7354773B2 (en) * | 2003-05-14 | 2008-04-08 | Beckman Coulter, Inc. | Method and apparatus for preparing cell samples for intracellular antigen detection using flow cytometry |
JP2005274425A (ja) * | 2004-03-25 | 2005-10-06 | New Industry Research Organization | Pxプローブを用いたフォスファチジルイノシトール3リン酸の検出方法 |
US20050266503A1 (en) * | 2004-05-24 | 2005-12-01 | Esoterix, Inc. | Simultaneous assay of target and target-drug binding |
US7803523B2 (en) | 2004-08-27 | 2010-09-28 | University Health Network | Whole blood preparation for cytometric analysis of cell signaling pathways |
US7678578B2 (en) * | 2005-02-07 | 2010-03-16 | Beckman Coulter, Inc. | Cell permeabilization and stabilization reagent and method of use |
JP5252682B2 (ja) * | 2005-02-25 | 2013-07-31 | 学校法人日本大学 | 哺乳動物t細胞のサイトカインの分析方法と関連する一切の疾患の評価法と予防・治療の分析 |
US7235404B2 (en) * | 2005-05-04 | 2007-06-26 | Beckman Coulter, Inc. | Cyanide-free lytic reagent composition and method of use for hemoglobin and white blood cell measurement |
TWI333959B (en) * | 2005-08-31 | 2010-12-01 | Academia Sinica | Methods and reagents for the analysis and purification of polysaccharides |
US7625757B2 (en) * | 2006-01-27 | 2009-12-01 | Sysmex Corporation | Reagent for immature leukocyte analysis and reagent kit |
JP4976038B2 (ja) * | 2006-03-29 | 2012-07-18 | シスメックス株式会社 | 血液学的試料の測定方法 |
HU0600578D0 (en) * | 2006-07-13 | 2006-09-28 | Szilak Labor Bioinformatikai E | Nuclear protein transport |
RU2435164C2 (ru) * | 2007-06-25 | 2011-11-27 | Сисмекс Корпорейшн | Реактив и набор реактивов для анализа незрелых лейкоцитов |
WO2009001869A1 (ja) * | 2007-06-25 | 2008-12-31 | Sysmex Corporation | 幼若白血球の分析用試薬及び分析用試薬キット |
GB2455204B (en) * | 2007-11-28 | 2010-07-14 | Smart Tube Inc | Devices, systems and methods for the collection, stimulation, stabilization and analysis of a biological sample |
EP3104177B1 (en) | 2011-12-21 | 2020-11-25 | Beckman Coulter, Inc. | Method for labeling intracellular and extracellular targets of leukocytes |
-
2011
- 2011-12-21 EP EP16001447.8A patent/EP3104177B1/en active Active
- 2011-12-21 EP EP11290586.4A patent/EP2607899B1/en active Active
-
2012
- 2012-12-21 US US14/366,024 patent/US9678073B2/en active Active
- 2012-12-21 JP JP2014548248A patent/JP6053818B2/ja active Active
- 2012-12-21 AU AU2012356324A patent/AU2012356324A1/en not_active Abandoned
- 2012-12-21 KR KR1020147016948A patent/KR102067742B1/ko active IP Right Grant
- 2012-12-21 WO PCT/IB2012/003071 patent/WO2013093642A1/en active Application Filing
- 2012-12-21 CA CA2859605A patent/CA2859605C/en active Active
- 2012-12-21 BR BR112014015204-7A patent/BR112014015204B1/pt active IP Right Grant
- 2012-12-21 CN CN201280063544.1A patent/CN104011542B/zh active Active
- 2012-12-21 MX MX2014007284A patent/MX352837B/es active IP Right Grant
-
2016
- 2016-06-21 US US15/188,856 patent/US11635433B2/en active Active
-
2018
- 2018-10-11 AU AU2018247279A patent/AU2018247279B2/en active Active
-
2023
- 2023-03-21 US US18/124,224 patent/US20230288416A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
US11635433B2 (en) | 2023-04-25 |
BR112014015204B1 (pt) | 2021-03-30 |
KR102067742B1 (ko) | 2020-01-20 |
JP6053818B2 (ja) | 2016-12-27 |
CN104011542B (zh) | 2016-04-27 |
AU2012356324A1 (en) | 2014-07-03 |
KR20140100536A (ko) | 2014-08-14 |
US9678073B2 (en) | 2017-06-13 |
WO2013093642A1 (en) | 2013-06-27 |
CA2859605C (en) | 2020-07-07 |
BR112014015204A2 (pt) | 2017-06-13 |
US20150010923A1 (en) | 2015-01-08 |
EP2607899B1 (en) | 2016-08-10 |
MX2014007284A (es) | 2015-10-29 |
EP3104177B1 (en) | 2020-11-25 |
JP2015500999A (ja) | 2015-01-08 |
US20230288416A1 (en) | 2023-09-14 |
AU2018247279B2 (en) | 2020-12-24 |
EP3104177A1 (en) | 2016-12-14 |
US20160299139A1 (en) | 2016-10-13 |
CN104011542A (zh) | 2014-08-27 |
EP2607899A1 (en) | 2013-06-26 |
AU2018247279A1 (en) | 2018-11-01 |
BR112014015204A8 (pt) | 2017-06-13 |
CA2859605A1 (en) | 2013-06-27 |
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Legal Events
Date | Code | Title | Description |
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FG | Grant or registration |