BR112014015204A8 - método para a marcação de alvos intracelulares e extracelulares de leucócitos - Google Patents

método para a marcação de alvos intracelulares e extracelulares de leucócitos

Info

Publication number
BR112014015204A8
BR112014015204A8 BR112014015204A BR112014015204A BR112014015204A8 BR 112014015204 A8 BR112014015204 A8 BR 112014015204A8 BR 112014015204 A BR112014015204 A BR 112014015204A BR 112014015204 A BR112014015204 A BR 112014015204A BR 112014015204 A8 BR112014015204 A8 BR 112014015204A8
Authority
BR
Brazil
Prior art keywords
intracellular
extracellular
marking
targets
leukocyte
Prior art date
Application number
BR112014015204A
Other languages
English (en)
Other versions
BR112014015204B1 (pt
BR112014015204A2 (pt
Inventor
Van Agthoven Andreas
Malergue Fabrice
Original Assignee
Beckman Coulter Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Coulter Inc filed Critical Beckman Coulter Inc
Publication of BR112014015204A2 publication Critical patent/BR112014015204A2/pt
Publication of BR112014015204A8 publication Critical patent/BR112014015204A8/pt
Publication of BR112014015204B1 publication Critical patent/BR112014015204B1/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G01N2333/70514—CD4
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70589—CD45
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70596—Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • Physiology (AREA)
  • Ecology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

resumo patente de invenção: "método para a marcação de alvos intracelulares e extracelulares de leucócitos". a presente invenção refere-se a métodos para a marcação de alvos intracelulares e extracelulares de leucócitos, bem como a kits para a realização dos ditos métodos, pois o manchamento de leucócitos com agentes de ligação, tais como anticorpos monoclonais (mabs), é uma importante tarefa que é rotineiramente realizada tanto em pesquisa básica bem como em uma variedade de aplicações de diagnóstico. muito frequentemente, marcadores extracelulares bem como intracelulares dos leucócitos têm de ser manchados, por exemplo, a fim de identificar um subconjunto particular de leucócitos por marcadores de superfície extracelulares específicos, bem como características funcionais das células por moléculas específicas que estão presentes no citoplasma. 4/4 1/1
BR112014015204-7A 2011-12-21 2012-12-21 Método para a marcação de alvos intracelulares e extracelulares de leucócitos BR112014015204B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11290586.4 2011-12-21
EP11290586.4A EP2607899B1 (en) 2011-12-21 2011-12-21 Method for labeling intracellular and extracellular targets of leukocytes
PCT/IB2012/003071 WO2013093642A1 (en) 2011-12-21 2012-12-21 Method for labeling intracellular and extracellular targets of leukocytes

Publications (3)

Publication Number Publication Date
BR112014015204A2 BR112014015204A2 (pt) 2017-06-13
BR112014015204A8 true BR112014015204A8 (pt) 2017-06-13
BR112014015204B1 BR112014015204B1 (pt) 2021-03-30

Family

ID=47563336

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014015204-7A BR112014015204B1 (pt) 2011-12-21 2012-12-21 Método para a marcação de alvos intracelulares e extracelulares de leucócitos

Country Status (10)

Country Link
US (3) US9678073B2 (pt)
EP (2) EP3104177B1 (pt)
JP (1) JP6053818B2 (pt)
KR (1) KR102067742B1 (pt)
CN (1) CN104011542B (pt)
AU (2) AU2012356324A1 (pt)
BR (1) BR112014015204B1 (pt)
CA (1) CA2859605C (pt)
MX (1) MX352837B (pt)
WO (1) WO2013093642A1 (pt)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3104177B1 (en) 2011-12-21 2020-11-25 Beckman Coulter, Inc. Method for labeling intracellular and extracellular targets of leukocytes
US20170097361A1 (en) 2015-07-15 2017-04-06 Streck, Inc. Method and device for stabilizing of proteins
WO2017141789A1 (ja) * 2016-02-19 2017-08-24 コニカミノルタ株式会社 血液由来検体の保管方法および希少細胞の判定方法
WO2020014639A1 (en) 2018-07-12 2020-01-16 Beckman Coulter, Inc. Fixable viability dyes and their uses
CN111807809B (zh) * 2019-04-10 2021-12-28 盐城工业职业技术学院 一种棕榈纳米纤维-石墨烯-碳纳米管复合气凝胶的制备方法
CN113884671A (zh) * 2021-09-27 2022-01-04 苏州东岭生物技术有限公司 一种流式染色试剂盒及其配置方法和应用方法

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6179163A (ja) * 1984-09-26 1986-04-22 Toa Medical Electronics Co Ltd 血球測定用試薬
JP2891302B2 (ja) * 1990-03-01 1999-05-17 シスメックス株式会社 血液中の白血球およびヘモグロビンの測定用試薬
DE69113445T2 (de) * 1990-12-08 1996-05-09 Mory Suntour Inc Fahrradgangschaltung.
US5422277A (en) * 1992-03-27 1995-06-06 Ortho Diagnostic Systems Inc. Cell fixative composition and method of staining cells without destroying the cell surface
AU6235994A (en) * 1993-02-25 1994-09-14 Abbott Laboratories Multipurpose reagent system for rapid lysis of whole blood samples
US5891734A (en) * 1994-08-01 1999-04-06 Abbott Laboratories Method for performing automated analysis
JP3170176B2 (ja) * 1995-05-17 2001-05-28 大塚製薬株式会社 薬剤耐性獲得癌細胞の検出方法
EP0900367A1 (en) * 1996-04-12 1999-03-10 Oncor, Inc. Method and composition for controlling formaldehyde fixation by delayed quenching
ATE253223T1 (de) * 1998-08-27 2003-11-15 Mayo Foundation Verfahren und materialien zur diagnose instabiler angina
US6210969B1 (en) * 1999-04-28 2001-04-03 Coulter International Corp. Composition and method for differentiation of basophil and eosinophil subpopulations of leukocytes in blood
US7354773B2 (en) * 2003-05-14 2008-04-08 Beckman Coulter, Inc. Method and apparatus for preparing cell samples for intracellular antigen detection using flow cytometry
JP2005274425A (ja) * 2004-03-25 2005-10-06 New Industry Research Organization Ｐｘプローブを用いたフォスファチジルイノシトール３リン酸の検出方法
US20050266503A1 (en) * 2004-05-24 2005-12-01 Esoterix, Inc. Simultaneous assay of target and target-drug binding
US7803523B2 (en) * 2004-08-27 2010-09-28 University Health Network Whole blood preparation for cytometric analysis of cell signaling pathways
US7678578B2 (en) * 2005-02-07 2010-03-16 Beckman Coulter, Inc. Cell permeabilization and stabilization reagent and method of use
WO2006090874A1 (ja) * 2005-02-25 2006-08-31 Nihon University 哺乳動物ｔ細胞のサイトカインの分析方法と関連する一切の疾患の評価法と予防・治療の分析
US7235404B2 (en) * 2005-05-04 2007-06-26 Beckman Coulter, Inc. Cyanide-free lytic reagent composition and method of use for hemoglobin and white blood cell measurement
TWI333959B (en) * 2005-08-31 2010-12-01 Academia Sinica Methods and reagents for the analysis and purification of polysaccharides
US7625757B2 (en) * 2006-01-27 2009-12-01 Sysmex Corporation Reagent for immature leukocyte analysis and reagent kit
JP4976038B2 (ja) * 2006-03-29 2012-07-18 シスメックス株式会社 血液学的試料の測定方法
HU0600578D0 (en) * 2006-07-13 2006-09-28 Szilak Labor Bioinformatikai E Nuclear protein transport
RU2435164C2 (ru) * 2007-06-25 2011-11-27 Сисмекс Корпорейшн Реактив и набор реактивов для анализа незрелых лейкоцитов
EP2166354B1 (en) * 2007-06-25 2012-11-07 Sysmex Corporation Reagent and reagent kit for analysis of primitive leukocyte
CN101896276A (zh) * 2007-11-28 2010-11-24 智能管公司 用于生物标本的收集、刺激、稳定化和分析的装置、系统和方法
EP3104177B1 (en) 2011-12-21 2020-11-25 Beckman Coulter, Inc. Method for labeling intracellular and extracellular targets of leukocytes

Also Published As

Publication number Publication date
US20160299139A1 (en) 2016-10-13
CA2859605C (en) 2020-07-07
BR112014015204B1 (pt) 2021-03-30
EP3104177A1 (en) 2016-12-14
US20230288416A1 (en) 2023-09-14
EP2607899B1 (en) 2016-08-10
US9678073B2 (en) 2017-06-13
AU2012356324A1 (en) 2014-07-03
KR20140100536A (ko) 2014-08-14
CN104011542B (zh) 2016-04-27
EP3104177B1 (en) 2020-11-25
US20150010923A1 (en) 2015-01-08
MX2014007284A (es) 2015-10-29
AU2018247279A1 (en) 2018-11-01
KR102067742B1 (ko) 2020-01-20
WO2013093642A1 (en) 2013-06-27
MX352837B (es) 2017-12-11
EP2607899A1 (en) 2013-06-26
JP6053818B2 (ja) 2016-12-27
JP2015500999A (ja) 2015-01-08
CA2859605A1 (en) 2013-06-27
BR112014015204A2 (pt) 2017-06-13
US11635433B2 (en) 2023-04-25
CN104011542A (zh) 2014-08-27
AU2018247279B2 (en) 2020-12-24

Similar Documents

Publication Publication Date Title
BR112014015204A8 (pt) método para a marcação de alvos intracelulares e extracelulares de leucócitos
BR112018010933A2 (pt) anticorpos multiespecíficos
BR112017027498A2 (pt) anticorpo, ácido nucleico, composto, composição, e, método para tratamento de um paciente com uma doença.
AR118722A2 (es) Proteínas tóxicas para especies de insectos hemípteros
BR112015014751A2 (pt) anticorpos anti-tau humanos
BR112019000512A2 (pt) anticorpo, ácido nucleico, vetor de expressão, célula hospedeira, composição, métodos de tratamento de uma doença, para produzir um anticorpo biespecífico e para detectar se a reticulação entre as células que expressam cd40 e cd137 ocorre em uma amostra, uso de um anticorpo multiespecífico, e, kit
NZ709167A (en) Compositions and methods for diagnosing thyroid tumors
BR112014012101A2 (pt) métodos de tratamento de artrite psoriática (psa) usando os antagonistas de il- 17 e alelos de resposta ou não resposta à psa
BR112015022322A2 (pt) sistemas e métodos para detecção de células com o uso de partículas de transdução projetadas
BR112016015677A2 (pt) Moléculas de anticorpo isoladas capazes de se ligarem à pd-1 humana, bem como seu método de produção e seu uso, composições farmacêuticas, ácido nucleico, vetor de expressão, célula hospedeira, e método para detecção de pd-1 em uma amostra biológica
BR112017025051A2 (pt) ensaio celular para detecção de homodímeros anti-cd3
SI2925888T1 (en) Compounds and methods for the treatment of cancer
AR085138A1 (es) VARIANTES DE Fc Y METODOS PARA SU PRODUCCION
ECSP14001249A (es) Inmunoenlazadores dirigidos contra el tnf
BR112012008665A2 (pt) tratamento de câncer
AR089393A1 (es) Composiciones y metodos para anticuerpos que actuan sobre el factor p
MX2017000083A (es) Compuestos de platino, composiciones y usos de estos.
BR112014003877A2 (pt) biomarcadores de micro-rna e métodos de uso dos mesmos
BR112017009151A2 (pt) ensaios para detectar subgrupos imunológicos de célula t e métodos de uso dos mesmos
BR112015021189A2 (pt) métodos de banco de células de alta densidade
BR112014000181A2 (pt) bloqueamento de anticorpo agr2 e uso dos mesmos
BR112013024148A2 (pt) dispositivos e métodos para separar partes marcadas magneticamente em uma amostra
BR112014009962A2 (pt) anticorpos humanizados para inkt
BR112014032892A2 (pt) processo de amplificação por rcp e de detecção de pelo menos uma sequência-alvo nucleotídica, dispositivo de amplificação e de detecção rápida de pelo menos uma sequência-alvo nucleotídica e estojo de amplificação por rcp e de detecção de pelo menos uma sequência nucleotídica-alvo
BR112013030875A2 (pt) "anticorpos anti-cd52 humanizados, polinucleotídeo codificando os mesmos, vetor, célula hospedeira, composição compreendendo os mesmos, método para detecção da presença de antígeno de cd52 humana em uma amostra, bem como uso dos referidos anticorpos, polinucleotídeo e vetor"

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 21/12/2012, OBSERVADAS AS CONDICOES LEGAIS.