BR112014015204A8 - method for marking intracellular and extracellular leukocyte targets - Google Patents

method for marking intracellular and extracellular leukocyte targets

Info

Publication number
BR112014015204A8
BR112014015204A8 BR112014015204A BR112014015204A BR112014015204A8 BR 112014015204 A8 BR112014015204 A8 BR 112014015204A8 BR 112014015204 A BR112014015204 A BR 112014015204A BR 112014015204 A BR112014015204 A BR 112014015204A BR 112014015204 A8 BR112014015204 A8 BR 112014015204A8
Authority
BR
Brazil
Prior art keywords
intracellular
extracellular
marking
targets
leukocyte
Prior art date
Application number
BR112014015204A
Other languages
Portuguese (pt)
Other versions
BR112014015204A2 (en
BR112014015204B1 (en
Inventor
Van Agthoven Andreas
Malergue Fabrice
Original Assignee
Beckman Coulter Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Coulter Inc filed Critical Beckman Coulter Inc
Publication of BR112014015204A8 publication Critical patent/BR112014015204A8/en
Publication of BR112014015204A2 publication Critical patent/BR112014015204A2/en
Publication of BR112014015204B1 publication Critical patent/BR112014015204B1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G01N2333/70514—CD4
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70589—CD45
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70596—Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • Ecology (AREA)
  • Physiology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

resumo patente de invenção: "método para a marcação de alvos intracelulares e extracelulares de leucócitos". a presente invenção refere-se a métodos para a marcação de alvos intracelulares e extracelulares de leucócitos, bem como a kits para a realização dos ditos métodos, pois o manchamento de leucócitos com agentes de ligação, tais como anticorpos monoclonais (mabs), é uma importante tarefa que é rotineiramente realizada tanto em pesquisa básica bem como em uma variedade de aplicações de diagnóstico. muito frequentemente, marcadores extracelulares bem como intracelulares dos leucócitos têm de ser manchados, por exemplo, a fim de identificar um subconjunto particular de leucócitos por marcadores de superfície extracelulares específicos, bem como características funcionais das células por moléculas específicas que estão presentes no citoplasma. 4/4 1/1patent summary: "method for marking intracellular and extracellular leukocyte targets". The present invention relates to methods for marking intracellular and extracellular leukocyte targets, as well as kits for performing said methods, since leukocyte staining with binding agents such as monoclonal antibodies (mabs) is a This is an important task that is routinely performed in both basic research and a variety of diagnostic applications. very often extracellular as well as intracellular markers of leukocytes have to be stained, for example, in order to identify a particular subset of leukocytes by specific extracellular surface markers, as well as functional characteristics of cells by specific molecules that are present in the cytoplasm. 4/4 1/1

BR112014015204-7A 2011-12-21 2012-12-21 METHOD FOR THE MARKING OF INTRACELLULAR AND EXTRACELLULAR TARGETS OF LEUKOCYTES BR112014015204B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11290586.4 2011-12-21
EP11290586.4A EP2607899B1 (en) 2011-12-21 2011-12-21 Method for labeling intracellular and extracellular targets of leukocytes
PCT/IB2012/003071 WO2013093642A1 (en) 2011-12-21 2012-12-21 Method for labeling intracellular and extracellular targets of leukocytes

Publications (3)

Publication Number Publication Date
BR112014015204A8 true BR112014015204A8 (en) 2017-06-13
BR112014015204A2 BR112014015204A2 (en) 2017-06-13
BR112014015204B1 BR112014015204B1 (en) 2021-03-30

Family

ID=47563336

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014015204-7A BR112014015204B1 (en) 2011-12-21 2012-12-21 METHOD FOR THE MARKING OF INTRACELLULAR AND EXTRACELLULAR TARGETS OF LEUKOCYTES

Country Status (10)

Country Link
US (3) US9678073B2 (en)
EP (2) EP2607899B1 (en)
JP (1) JP6053818B2 (en)
KR (1) KR102067742B1 (en)
CN (1) CN104011542B (en)
AU (2) AU2012356324A1 (en)
BR (1) BR112014015204B1 (en)
CA (1) CA2859605C (en)
MX (1) MX352837B (en)
WO (1) WO2013093642A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2607899B1 (en) 2011-12-21 2016-08-10 Beckman Coulter, Inc. Method for labeling intracellular and extracellular targets of leukocytes
US20170097361A1 (en) 2015-07-15 2017-04-06 Streck, Inc. Method and device for stabilizing of proteins
JPWO2017141789A1 (en) * 2016-02-19 2018-12-13 コニカミノルタ株式会社 Storage method of blood-derived specimen and determination method of rare cells
JP7076016B2 (en) * 2018-07-12 2022-05-26 ベックマン コールター， インコーポレイテッド Immobilable viability analysis dyes and their use
CN111807809B (en) * 2019-04-10 2021-12-28 盐城工业职业技术学院 Preparation method of palm nanofiber-graphene-carbon nanotube composite aerogel
CN113884671A (en) * 2021-09-27 2022-01-04 苏州东岭生物技术有限公司 Flow type dyeing kit and configuration method and application method thereof

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EP2607899B1 (en) 2011-12-21 2016-08-10 Beckman Coulter, Inc. Method for labeling intracellular and extracellular targets of leukocytes

Also Published As

Publication number Publication date
US20230288416A1 (en) 2023-09-14
US11635433B2 (en) 2023-04-25
US9678073B2 (en) 2017-06-13
EP3104177B1 (en) 2020-11-25
EP3104177A1 (en) 2016-12-14
EP2607899A1 (en) 2013-06-26
MX2014007284A (en) 2015-10-29
AU2018247279B2 (en) 2020-12-24
WO2013093642A1 (en) 2013-06-27
EP2607899B1 (en) 2016-08-10
BR112014015204A2 (en) 2017-06-13
US20160299139A1 (en) 2016-10-13
US20150010923A1 (en) 2015-01-08
AU2012356324A1 (en) 2014-07-03
AU2018247279A1 (en) 2018-11-01
JP2015500999A (en) 2015-01-08
JP6053818B2 (en) 2016-12-27
CA2859605A1 (en) 2013-06-27
CN104011542A (en) 2014-08-27
CN104011542B (en) 2016-04-27
CA2859605C (en) 2020-07-07
KR20140100536A (en) 2014-08-14
MX352837B (en) 2017-12-11
BR112014015204B1 (en) 2021-03-30
KR102067742B1 (en) 2020-01-20

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 21/12/2012, OBSERVADAS AS CONDICOES LEGAIS.