DE60230858D1 - Chemisch definiertes medium für kultivierte säugerzellen - Google Patents
Chemisch definiertes medium für kultivierte säugerzellenInfo
- Publication number
- DE60230858D1 DE60230858D1 DE60230858T DE60230858T DE60230858D1 DE 60230858 D1 DE60230858 D1 DE 60230858D1 DE 60230858 T DE60230858 T DE 60230858T DE 60230858 T DE60230858 T DE 60230858T DE 60230858 D1 DE60230858 D1 DE 60230858D1
- Authority
- DE
- Germany
- Prior art keywords
- mammalian cells
- chemically defined
- defined medium
- cultivated mammalian
- cultivated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/34—Sugars
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/04—Immortalised cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicinal Preparation (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US26884901P | 2001-02-15 | 2001-02-15 | |
PCT/US2002/003274 WO2002066603A2 (en) | 2001-02-15 | 2002-02-05 | Chemically defined medium for cultured mammalian cells |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60230858D1 true DE60230858D1 (de) | 2009-03-05 |
Family
ID=23024766
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60230858T Expired - Lifetime DE60230858D1 (de) | 2001-02-15 | 2002-02-05 | Chemisch definiertes medium für kultivierte säugerzellen |
Country Status (7)
Country | Link |
---|---|
US (1) | US6900056B2 (de) |
EP (1) | EP1360314B1 (de) |
JP (2) | JP2005505240A (de) |
AT (1) | ATE420945T1 (de) |
CA (1) | CA2438148A1 (de) |
DE (1) | DE60230858D1 (de) |
WO (1) | WO2002066603A2 (de) |
Families Citing this family (55)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003052064A2 (en) * | 2001-12-14 | 2003-06-26 | Centocor Inc. | Clonal myeloma cell lines useful for manufacturing proteins in chemically defined media |
CA2417689C (en) * | 2002-03-05 | 2006-05-09 | F. Hoffmann-La Roche Ag | Improved methods for growing mammalian cells in vitro |
WO2005028626A2 (en) * | 2003-09-18 | 2005-03-31 | Raven Biotechnologies, Inc. | Cell culture media |
DE602004026917D1 (de) * | 2003-12-26 | 2010-06-10 | Makoto Asashima | Basalmedium zur kultivierung von es-zellen |
WO2006024966A2 (en) * | 2004-09-02 | 2006-03-09 | Angela Cruz | Three-dimensional self assembly in suspension of adherent cells |
US8273553B2 (en) * | 2004-11-02 | 2012-09-25 | Ares Trading S.A. | Production of growth hormone in serum-free cell culture medium for mammalian cells |
DK1807504T3 (da) * | 2004-11-02 | 2011-03-21 | Ares Trading Sa | Serumfrit cellekulturmedium til celler fra pattedyr |
US20090214528A1 (en) | 2005-08-31 | 2009-08-27 | Haimanti Dorai | Host cell lines for production of antibody constant region with enhanced effector function |
DE102005046225B4 (de) * | 2005-09-28 | 2012-01-05 | Cellca Gmbh | Verbessertes Zellkulturmedium |
CN105567627B (zh) * | 2007-04-26 | 2020-09-29 | 中外制药株式会社 | 使用含高浓度氨基酸的培养基的细胞培养方法 |
EP2152856B1 (de) | 2007-05-11 | 2012-08-29 | Amgen Inc. | Verbesserte zufuhrmedien |
US7723491B1 (en) | 2007-05-25 | 2010-05-25 | Clemson University | Methods of isolating specific compounds from supramammary lymph node tissue |
US8637312B2 (en) * | 2008-01-09 | 2014-01-28 | Cellca Gmbh | Mammalian culture media with polyamine and iron |
WO2011019619A1 (en) * | 2009-08-11 | 2011-02-17 | Genentech, Inc. | Production of proteins in glutamine-free cell culture media |
US8759098B2 (en) | 2009-12-04 | 2014-06-24 | Boston Biomedical Research Institute, Inc. | Method for cloning pluripotent stem cells |
MX2012012526A (es) | 2010-04-26 | 2012-11-23 | Novartis Ag | Medio de cultivo celular mejorado. |
WO2012149197A2 (en) | 2011-04-27 | 2012-11-01 | Abbott Laboratories | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
JP6236383B2 (ja) | 2011-05-13 | 2017-11-22 | バイオジェン・エムエイ・インコーポレイテッドBiogen MA Inc. | トリスルフィド結合を防止および除去する方法 |
WO2013009648A2 (en) | 2011-07-08 | 2013-01-17 | Momenta Pharmaceuticals, Inc. | Cell culture process |
WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
US9334319B2 (en) | 2012-04-20 | 2016-05-10 | Abbvie Inc. | Low acidic species compositions |
US20150267237A1 (en) * | 2012-04-24 | 2015-09-24 | Genentech, Inc. | Cell culture compositions and methods for polypeptide production |
US20130281355A1 (en) * | 2012-04-24 | 2013-10-24 | Genentech, Inc. | Cell culture compositions and methods for polypeptide production |
US9249182B2 (en) | 2012-05-24 | 2016-02-02 | Abbvie, Inc. | Purification of antibodies using hydrophobic interaction chromatography |
EP2890782A1 (de) | 2012-09-02 | 2015-07-08 | AbbVie Inc. | Verfahren zur steuerung einer proteinheterogenität |
US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
NO2760138T3 (de) * | 2012-10-01 | 2018-08-04 | ||
US20140170748A1 (en) * | 2012-12-14 | 2014-06-19 | DePuy Synthes Products, LLC | Nutrient Enriched Media for hUTC Growth |
CA2905010A1 (en) | 2013-03-12 | 2014-09-18 | Abbvie Inc. | Human antibodies that bind human tnf-alpha and methods of preparing the same |
US9611455B2 (en) | 2013-03-13 | 2017-04-04 | Merck Sharp & Dohme Corp. | Adapted lepidopteran insect cells for the production of recombinant proteins |
WO2014159579A1 (en) | 2013-03-14 | 2014-10-02 | Abbvie Inc. | MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE |
US9499614B2 (en) | 2013-03-14 | 2016-11-22 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides |
US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
EP3052640A2 (de) | 2013-10-04 | 2016-08-10 | AbbVie Inc. | Verwendung von metallionen zur modulation von proteinglykosylierungsprofilen rekombinanter proteine |
US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
WO2015073884A2 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
BR112016017660B1 (pt) | 2014-02-27 | 2022-04-19 | F. Hoffmann-La Roche Ag | Método para a produção de uma glicoproteína recombinante |
WO2015137640A1 (ko) * | 2014-03-13 | 2015-09-17 | 한양대학교 산학협력단 | 화학 조성 세포 배양 배지 첨가물 |
KR101714860B1 (ko) | 2014-03-13 | 2017-03-09 | 한양대학교 산학협력단 | 화학 조성 세포 배양 배지 첨가물 |
US10227559B2 (en) * | 2014-04-10 | 2019-03-12 | Bayer Healthcare Llc | Compounded media powder formulation and method of preparation of liquid medium for cell culture |
US10564148B2 (en) | 2014-09-24 | 2020-02-18 | Triad National Security, Llc | Multi-organ media compositions and methods of their use |
EP3090738A1 (de) * | 2015-05-04 | 2016-11-09 | Universidade de Santiago de Compostela | Riboflavin zur behandlung ischämischer schlaganfälle und/oder anderer glutamatexzitotoxizität-assoziierter erkrankungen |
EP3384004A1 (de) * | 2015-12-03 | 2018-10-10 | Merck Patent GmbH | Chemisch definierte medien zum wachstum oder nachweis von mikroorganismen |
WO2017123465A1 (en) | 2016-01-14 | 2017-07-20 | DePuy Synthes Products, Inc. | Composition and methods for cryopreservation of hutc |
EP3440190B1 (de) | 2016-04-05 | 2024-08-28 | Pfizer Inc. | Zellkulturverfahren |
JP2020127383A (ja) * | 2019-02-08 | 2020-08-27 | 学校法人 東洋大学 | 培養方法、培養装置、廃水処理方法及び廃水処理装置 |
CA3129732A1 (en) * | 2019-02-14 | 2020-08-20 | North Grove Investments, Inc. | Compositions for maintaining the viability of living and static biological material, methods of making and the uses thereof |
CN110241090B (zh) * | 2019-05-07 | 2023-10-13 | 江苏南农高科技股份有限公司 | 一种全悬浮细胞培养生产猪伪狂犬病毒抗原的方法 |
BR112021025432A2 (pt) | 2019-12-06 | 2022-06-21 | Regeneron Pharma | Composições de proteína anti-vegf e métodos para a produção das mesmas |
JP2022060169A (ja) | 2020-10-02 | 2022-04-14 | ファイザー・インク | Rsv fタンパク質生産のための細胞培養工程 |
WO2022254319A1 (en) | 2021-06-01 | 2022-12-08 | Pfizer Inc. | Cell culture method for producing sfgfr3 polypeptide |
CN115386534B (zh) * | 2022-10-26 | 2023-02-21 | 天信和(苏州)生物科技有限公司 | 利用无血清培养基培养bhk21细胞的方法及其在制备疫苗中的用途 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4205126A (en) * | 1978-01-01 | 1980-05-27 | Cartaya Oscar A | Serum-free cell culture media |
US4767704A (en) * | 1983-10-07 | 1988-08-30 | Columbia University In The City Of New York | Protein-free culture medium |
CS262822B1 (en) * | 1986-10-03 | 1989-04-14 | Kovar Jan | Synthetic medium for the cultivation of myelomic cells |
JPH078273A (ja) * | 1993-06-14 | 1995-01-13 | Kurabo Ind Ltd | 接着性動物細胞の無血清培養法 |
US5650317A (en) | 1994-09-16 | 1997-07-22 | Michigan State University | Human breast epithelial cell type with stem cell and luminal epithelial cell characteristics |
ES2180689T3 (es) * | 1996-04-19 | 2003-02-16 | Nestle Sa | Linea inmortalizada de celulas epiteliales del colon humano. |
EP2221361A3 (de) * | 1996-08-30 | 2011-02-09 | Life Technologies Corporation | Methode zur Herstellung eines Polypeptids in vitro in einer Säugerzelle in einem Kulturmedium ohne Serum und ohne Proteine |
WO1998016629A1 (en) * | 1996-10-11 | 1998-04-23 | Life Technologies, Inc. | Defined systems for epithelial cell culture and use thereof |
WO2002101019A2 (en) | 2001-06-13 | 2002-12-19 | Genentech, Inc. | Methods of culturing animal cells and polypeptide production in animal cells |
-
2002
- 2002-02-05 AT AT02709335T patent/ATE420945T1/de not_active IP Right Cessation
- 2002-02-05 CA CA 2438148 patent/CA2438148A1/en not_active Abandoned
- 2002-02-05 EP EP02709335A patent/EP1360314B1/de not_active Expired - Lifetime
- 2002-02-05 WO PCT/US2002/003274 patent/WO2002066603A2/en active Application Filing
- 2002-02-05 US US10/067,382 patent/US6900056B2/en not_active Expired - Lifetime
- 2002-02-05 JP JP2002566310A patent/JP2005505240A/ja active Pending
- 2002-02-05 DE DE60230858T patent/DE60230858D1/de not_active Expired - Lifetime
-
2008
- 2008-03-25 JP JP2008077561A patent/JP2008178419A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
ATE420945T1 (de) | 2009-01-15 |
JP2005505240A (ja) | 2005-02-24 |
JP2008178419A (ja) | 2008-08-07 |
WO2002066603A3 (en) | 2002-12-19 |
EP1360314A2 (de) | 2003-11-12 |
US20030096402A1 (en) | 2003-05-22 |
EP1360314B1 (de) | 2009-01-14 |
US6900056B2 (en) | 2005-05-31 |
CA2438148A1 (en) | 2002-08-29 |
EP1360314A4 (de) | 2004-08-11 |
WO2002066603A2 (en) | 2002-08-29 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |