BR112012020964B8 - método para produzir uma imunoglobulina da subclasse igg1 ou igg4 - Google Patents

método para produzir uma imunoglobulina da subclasse igg1 ou igg4

Info

Publication number
BR112012020964B8
BR112012020964B8 BR112012020964A BR112012020964A BR112012020964B8 BR 112012020964 B8 BR112012020964 B8 BR 112012020964B8 BR 112012020964 A BR112012020964 A BR 112012020964A BR 112012020964 A BR112012020964 A BR 112012020964A BR 112012020964 B8 BR112012020964 B8 BR 112012020964B8
Authority
BR
Brazil
Prior art keywords
host cell
immunoglobulin
producing
igg1
protein
Prior art date
Application number
BR112012020964A
Other languages
English (en)
Other versions
BR112012020964A2 (pt
BR112012020964B1 (pt
Inventor
Hakemeyer Christian
Schwarz Felizitas
Binder Vinzenz
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42236585&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112012020964(B8) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of BR112012020964A2 publication Critical patent/BR112012020964A2/pt
Publication of BR112012020964B1 publication Critical patent/BR112012020964B1/pt
Publication of BR112012020964B8 publication Critical patent/BR112012020964B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

método para produzir uma imunoglobulina. e relatado no presente um método de purificação de sobrenadantes de cultivo celular tanto diretamente após a fermentação ou apés uma ou mais etapas de purificação preliminares, tais como cromatografia de afinidade de proteína a. por meio de ajuste do valor de ph na faixa acida e subsequente incubação da solução acidificada de ácido nucleico da célula hospedeira e a proteína da célula hospedeira pode ser precipitada, mas o polipeptídeo alvo permanece em solução. em seguida o precipitado e com ele os componentes de células hospedeiras contaminantes podem ser removidos por meio de uma etapa simples de separação física
BR112012020964A 2010-03-10 2011-03-09 método para produzir uma imunoglobulina da subclasse igg1 ou igg4 BR112012020964B8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10156103 2010-03-10
PCT/EP2011/053547 WO2011110598A1 (en) 2010-03-10 2011-03-09 Method for purifying immunoglobulin solutions

Publications (3)

Publication Number Publication Date
BR112012020964A2 BR112012020964A2 (pt) 2016-05-03
BR112012020964B1 BR112012020964B1 (pt) 2021-02-23
BR112012020964B8 true BR112012020964B8 (pt) 2021-08-24

Family

ID=42236585

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012020964A BR112012020964B8 (pt) 2010-03-10 2011-03-09 método para produzir uma imunoglobulina da subclasse igg1 ou igg4

Country Status (12)

Country Link
US (4) US9884904B2 (pt)
EP (1) EP2545066B1 (pt)
JP (2) JP5814951B2 (pt)
KR (1) KR101555740B1 (pt)
CN (1) CN102791726B (pt)
BR (1) BR112012020964B8 (pt)
CA (1) CA2789564C (pt)
ES (1) ES2648264T3 (pt)
HK (1) HK1176946A1 (pt)
MX (1) MX348739B (pt)
RU (1) RU2558367C2 (pt)
WO (1) WO2011110598A1 (pt)

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CA2789564C (en) * 2010-03-10 2019-08-13 F. Hoffmann-La Roche Ag Method for purifying immunoglobulin solutions
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
EP2726496B1 (en) 2011-07-01 2018-04-04 F.Hoffmann-La Roche Ag Method for separation of monomeric polypeptides from aggregated polypeptides
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
WO2013177118A2 (en) * 2012-05-21 2013-11-28 Abbvie Inc. Novel purification of non-human antibodies using protein a affinity chromatography
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
AU2013309506A1 (en) 2012-09-02 2015-03-12 Abbvie Inc. Methods to control protein heterogeneity
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
AU2013381687A1 (en) 2013-03-12 2015-09-24 Abbvie Inc. Human antibodies that bind human TNF-alpha and methods of preparing the same
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
EP3052640A2 (en) 2013-10-04 2016-08-10 AbbVie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
EP3118210B1 (en) * 2014-03-11 2019-11-13 Green Cross Holdings Corporation Method for purifying immunoglobulin
WO2016071376A2 (en) 2014-11-06 2016-05-12 F. Hoffmann-La Roche Ag Fc-region variants with modified fcrn-binding and methods of use
CN111378030A (zh) * 2018-12-29 2020-07-07 中国科学院青岛生物能源与过程研究所 一种纳米抗体分离纯化的方法
BR112022007929A2 (pt) * 2019-11-07 2022-07-12 Janssen Vaccines & Prevention Bv Purificação de proteínas

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US5196323A (en) * 1985-04-27 1993-03-23 Boehringer Ingelheim International Gmbh Process for preparing and purifying alpha-interferon
IL118069A (en) * 1995-05-02 2000-06-01 Japan Tobacco Inc Monoclonal antibody reactive to human CETP and assay method for human CETP
DE10037781A1 (de) * 2000-08-03 2002-02-21 Agfa Gevaert Ag Vorrichtung und Verfahren zum Beschreiben von Aufzeichnungsmaterial mit Bildinformationen
ES2184594B1 (es) * 2001-01-17 2004-01-01 Probitas Pharma Sa Procedimiento para la produccion de gammaglobulina g humana inactivada de virus.
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AU2003262087B2 (en) 2002-09-11 2010-11-11 Chugai Seiyaku Kabushiki Kaisha Protein purification method
ZA200504990B (en) * 2003-01-09 2006-08-30 Genentech Inc Purification of polypeptides
PL1737891T3 (pl) 2004-04-13 2013-08-30 Hoffmann La Roche Przeciwciała przeciw selektynie p
BRPI0609151B8 (pt) 2005-03-24 2021-05-25 Life Sciences Res Partners Vzw molécula de ligação ao antígeno capaz de se ligar a plgf, usos da mesma, linhagem celular de hibridoma, composição farmacêutica e polinucleotídeo
TWI320788B (en) 2005-05-25 2010-02-21 Hoffmann La Roche Method for the purification of antibodies
CN104926938B (zh) * 2005-06-17 2019-06-04 惠氏有限责任公司 纯化含Fc区蛋白的方法
AR062223A1 (es) 2006-08-09 2008-10-22 Glycart Biotechnology Ag Moleculas de adhesion al antigeno que se adhieren a egfr, vectores que los codifican, y sus usos de estas
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CA2789564C (en) * 2010-03-10 2019-08-13 F. Hoffmann-La Roche Ag Method for purifying immunoglobulin solutions
EP2726496B1 (en) 2011-07-01 2018-04-04 F.Hoffmann-La Roche Ag Method for separation of monomeric polypeptides from aggregated polypeptides

Also Published As

Publication number Publication date
US20190010216A1 (en) 2019-01-10
US20130005948A1 (en) 2013-01-03
JP5814951B2 (ja) 2015-11-17
US20230174625A1 (en) 2023-06-08
BR112012020964A2 (pt) 2016-05-03
CA2789564A1 (en) 2011-09-15
KR101555740B1 (ko) 2015-09-25
KR20120107140A (ko) 2012-09-28
US9884904B2 (en) 2018-02-06
MX348739B (es) 2017-06-27
BR112012020964B1 (pt) 2021-02-23
JP5931255B2 (ja) 2016-06-08
JP2013521323A (ja) 2013-06-10
CA2789564C (en) 2019-08-13
EP2545066A1 (en) 2013-01-16
HK1176946A1 (zh) 2013-08-09
WO2011110598A1 (en) 2011-09-15
CN102791726A (zh) 2012-11-21
US20200231655A1 (en) 2020-07-23
RU2012141149A (ru) 2014-04-20
EP2545066B1 (en) 2017-09-20
MX2012009892A (es) 2012-09-12
RU2558367C2 (ru) 2015-08-10
ES2648264T3 (es) 2017-12-29
CN102791726B (zh) 2016-02-03
JP2015213511A (ja) 2015-12-03

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
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Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NAO 10196/2001, QUE MODIFICOU A LEI NAO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUAANCIA PRA VIA DA ANVISA. CONSIDERANDO A APROVAA AO DOS TERMOS DO PARECER NAO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NAO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDAANCIAS CABA-VEIS.

B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
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