MX2013001447A - Metodo de produccion y purificacion de una sialiltransferasa soluble activa. - Google Patents
Metodo de produccion y purificacion de una sialiltransferasa soluble activa.Info
- Publication number
- MX2013001447A MX2013001447A MX2013001447A MX2013001447A MX2013001447A MX 2013001447 A MX2013001447 A MX 2013001447A MX 2013001447 A MX2013001447 A MX 2013001447A MX 2013001447 A MX2013001447 A MX 2013001447A MX 2013001447 A MX2013001447 A MX 2013001447A
- Authority
- MX
- Mexico
- Prior art keywords
- sialyltransferase
- polypeptide
- purifying
- producing
- active soluble
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
- C12Y204/99003—Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase (2.4.99.3)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
Abstract
La presente invención se relaciona con un método para la producción y purificación de un polipéptido de sialiltransferasa, en particular un polipéptido de N-Acetilgalactosamina (Gal NAc)-a-2, 6-sialiltransferasa I (ST6GalNAcI). El método comprende los pasos de producir el polipéptido de sialiltransferasa en una célula de Ovario de Hámster Chino (CHO) y purificar el polipéptido con una combinación de pasos cromatográficos. El método da por resultado en alto rendimiento del polipéptido de sialiltransferasa que es bastante pura y activa. La sialiltransferasa obtenida, en especial ST6GalNAcI, se puede emplear para la glucosilación de proteínas terapéuticas tales como G-CSF.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20100171626 EP2415779B1 (en) | 2010-08-02 | 2010-08-02 | Method of producing and purifying an active soluble sialyltransferase |
| PCT/EP2011/063291 WO2012016984A1 (en) | 2010-08-02 | 2011-08-02 | Method of producing and purifying an active soluble sialyltransferase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2013001447A true MX2013001447A (es) | 2013-11-20 |
Family
ID=42736163
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013001447A MX2013001447A (es) | 2010-08-02 | 2011-08-02 | Metodo de produccion y purificacion de una sialiltransferasa soluble activa. |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US9222081B2 (es) |
| EP (2) | EP2415779B1 (es) |
| JP (1) | JP5984267B2 (es) |
| CN (1) | CN103154017B (es) |
| CA (1) | CA2807397C (es) |
| ES (1) | ES2534936T3 (es) |
| MX (1) | MX2013001447A (es) |
| PT (1) | PT2415779E (es) |
| WO (1) | WO2012016984A1 (es) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013075740A1 (en) * | 2011-11-23 | 2013-05-30 | Sanofi | Antibody purification method |
| JP2016513245A (ja) * | 2013-02-06 | 2016-05-12 | エイジェンシー・フォー・サイエンス，テクノロジー・アンド・リサーチ | タンパク質製剤の凝集体含有量を減少させる混合多官能金属親和性表面 |
| JP6463734B2 (ja) | 2013-05-06 | 2019-02-06 | サノフイＳａｎｏｆｉ | 抗体を精製するための連続的多工程法 |
| RU2017135552A (ru) * | 2015-03-31 | 2019-04-30 | Иеда Рисеч Энд Девелопмент Ко. Лтд. | Глутамат оксалоацетат трансаминаза 1 (got1), препараты и способы их получения и применения |
| KR101667677B1 (ko) * | 2015-05-29 | 2016-10-19 | 한국생명공학연구원 | 재조합 ST6Gal1 단백질을 활성형으로 제조하는 방법 |
| AU2017381657B2 (en) | 2016-12-21 | 2020-07-23 | F. Hoffmann-La Roche Ag | Method for in vitro glycoengineering of antibodies |
| CA3043158A1 (en) * | 2016-12-21 | 2018-06-28 | F. Hoffmann-La Roche Ag | Re-use of enzymes in in vitro glycoengineering of antibodies |
| ES2930534T3 (es) | 2017-09-04 | 2022-12-16 | 89Bio Ltd | Conjugados de péptidos de FGF-21 mutantes y usos de los mismos |
| WO2019063849A1 (en) * | 2017-12-20 | 2019-04-04 | Dsm Ip Assets B.V. | PURIFICATION OF A POLYPEPTIDE OF INTEREST |
| KR102362777B1 (ko) * | 2018-03-27 | 2022-02-15 | 주식회사 녹십자 | 친화성 크로마토그래피 공정을 포함하는 백일해균 유래 단백질 수득 방법 |
| KR102140531B1 (ko) * | 2018-08-07 | 2020-08-04 | (주)휴온스 | Gly-Tβ4의 제조방법 |
| DK3613486T3 (da) * | 2018-08-24 | 2021-01-04 | Uga Biopharma Gmbh | Metode og anlæg til rengøring af epo og/eller et epo-derivat |
| CN113416243B (zh) * | 2021-08-23 | 2021-12-10 | 上海盛迪医药有限公司 | 一种纯化抗体的方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5032519A (en) | 1989-10-24 | 1991-07-16 | The Regents Of The Univ. Of California | Method for producing secretable glycosyltransferases and other Golgi processing enzymes |
| WO1995018217A1 (fr) | 1993-12-24 | 1995-07-06 | The Institute Of Physical And Chemical Research | Nouvelle synthetase a chaine de sucres, et procede pour produire cette derniere |
| US6280989B1 (en) * | 1999-06-17 | 2001-08-28 | Dmitri Kapitonov | Sialyltransferases |
| EP2279755B1 (en) | 2001-10-10 | 2014-02-26 | ratiopharm GmbH | Remodelling and glycoconjugation of Fibroblast Growth Factor (FGF) |
| EP1694274B1 (en) * | 2003-12-03 | 2013-04-24 | BioGeneriX AG | Glycopegylated granulocyte colony stimulating factor |
| US20080206810A1 (en) * | 2004-06-03 | 2008-08-28 | Neose Technologies, Inc. | Truncated St6galnaci Polypeptides and Nucleic Acids |
| ATE527371T1 (de) | 2005-03-24 | 2011-10-15 | Biogenerix Ag | Expression löslicher, aktiver, eukaryotischer glucosyltransferasen in prokaryotischen organismen |
| WO2006105426A2 (en) | 2005-03-30 | 2006-10-05 | Neose Technologies, Inc. | Manufacturing process for the production of peptides grown in insect cell lines |
| US20090047710A1 (en) | 2005-11-09 | 2009-02-19 | Neose Technologies, Inc. | ST3Gal-1/ST6GalNAc-1 Chimeras |
| WO2008057683A2 (en) * | 2006-10-03 | 2008-05-15 | Novo Nordisk A/S | Methods for the purification of polypeptide conjugates |
| US20080207487A1 (en) | 2006-11-02 | 2008-08-28 | Neose Technologies, Inc. | Manufacturing process for the production of polypeptides expressed in insect cell-lines |
-
2010
- 2010-08-02 EP EP20100171626 patent/EP2415779B1/en active Active
- 2010-08-02 ES ES10171626.4T patent/ES2534936T3/es active Active
- 2010-08-02 PT PT101716264T patent/PT2415779E/pt unknown
-
2011
- 2011-08-02 US US13/813,804 patent/US9222081B2/en active Active
- 2011-08-02 CA CA2807397A patent/CA2807397C/en active Active
- 2011-08-02 MX MX2013001447A patent/MX2013001447A/es unknown
- 2011-08-02 CN CN201180047627.7A patent/CN103154017B/zh not_active Expired - Fee Related
- 2011-08-02 WO PCT/EP2011/063291 patent/WO2012016984A1/en active Application Filing
- 2011-08-02 EP EP11738247.3A patent/EP2601207B1/en active Active
- 2011-08-02 JP JP2013522234A patent/JP5984267B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103154017B (zh) | 2016-02-03 |
| JP2013535207A (ja) | 2013-09-12 |
| EP2415779B1 (en) | 2015-01-21 |
| CA2807397A1 (en) | 2012-02-09 |
| EP2601207B1 (en) | 2016-12-28 |
| US20140057316A1 (en) | 2014-02-27 |
| CA2807397C (en) | 2019-03-19 |
| EP2415779A1 (en) | 2012-02-08 |
| US9222081B2 (en) | 2015-12-29 |
| ES2534936T3 (es) | 2015-04-30 |
| WO2012016984A1 (en) | 2012-02-09 |
| JP5984267B2 (ja) | 2016-09-06 |
| CN103154017A (zh) | 2013-06-12 |
| PT2415779E (pt) | 2015-05-13 |
| EP2601207A1 (en) | 2013-06-12 |
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