MX344815B - Metodo para purificar el factor estimulante de colonias de granulocitos humano a partir de e. coli recombinante. - Google Patents
Metodo para purificar el factor estimulante de colonias de granulocitos humano a partir de e. coli recombinante.Info
- Publication number
- MX344815B MX344815B MX2013004830A MX2013004830A MX344815B MX 344815 B MX344815 B MX 344815B MX 2013004830 A MX2013004830 A MX 2013004830A MX 2013004830 A MX2013004830 A MX 2013004830A MX 344815 B MX344815 B MX 344815B
- Authority
- MX
- Mexico
- Prior art keywords
- coli
- colony stimulating
- recombinant
- human granulocyte
- stimulating factor
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente invención se refiere a un método para purificar una gran cantidad de factores estimulantes de colonias de granulocitos humanos (hG-CSFs) a partir de un E. coli recombinante con alto rendimiento y pureza. De acuerdo con el método de la presente invención, el factor estimulante de colonias de granulocitos humano, idéntico a la forma nativa expresado en el cuerpo humano, se puede purificar fácilmente con alto rendimiento y pureza sin un proceso de activación adicional. En particular, de acuerdo con el método de purificación de la presente invención, las variantes de hG-CSF expresadas en E. coli se remueven eficientemente para obtener hG-CSFs fisiológicamente activos con alta pureza.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020100106994A KR101831300B1 (ko) | 2010-10-29 | 2010-10-29 | 재조합 대장균으로부터 인간 과립구 콜로니 자극인자를 정제하는 방법 |
| PCT/KR2011/008046 WO2012057529A2 (en) | 2010-10-29 | 2011-10-26 | Method for purifying human granulocyte-colony stimulating factor from recombinant e. coli |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2013004830A MX2013004830A (es) | 2013-06-03 |
| MX344815B true MX344815B (es) | 2017-01-06 |
Family
ID=45994563
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013004830A MX344815B (es) | 2010-10-29 | 2011-10-26 | Metodo para purificar el factor estimulante de colonias de granulocitos humano a partir de e. coli recombinante. |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US9193761B2 (es) |
| EP (1) | EP2632944B1 (es) |
| JP (2) | JP6138690B2 (es) |
| KR (1) | KR101831300B1 (es) |
| CN (1) | CN103228672B (es) |
| AU (1) | AU2011321169B2 (es) |
| BR (1) | BR112013010522B1 (es) |
| CA (1) | CA2816050C (es) |
| DK (1) | DK2632944T3 (es) |
| ES (1) | ES2640321T3 (es) |
| IL (1) | IL226043A (es) |
| MX (1) | MX344815B (es) |
| NZ (1) | NZ610314A (es) |
| RU (1) | RU2596408C2 (es) |
| SG (1) | SG189509A1 (es) |
| WO (1) | WO2012057529A2 (es) |
| ZA (1) | ZA201303834B (es) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112012017979B1 (pt) | 2010-01-19 | 2021-10-13 | Hanmi Science Co., Ltd. | Formulações líquidas para conjugado de g-csf de ação prolongada |
| KR101847405B1 (ko) | 2010-07-30 | 2018-04-10 | 이엠디 밀리포어 코포레이션 | 크로마토그래피 매질 및 방법 |
| WO2014155365A2 (en) * | 2013-03-29 | 2014-10-02 | Dr.Reddy's Laboratories Limited | Purification method |
| CN103233053B (zh) * | 2013-04-03 | 2014-03-05 | 北京四环生物制药有限公司 | 一种重组人粒细胞刺激因子的生产方法 |
| WO2016009451A2 (en) | 2014-07-14 | 2016-01-21 | Gennova Biopharmaceuticals Limited | A novel process for purification of rhu-gcsf |
| EP3230299A1 (en) | 2014-12-08 | 2017-10-18 | EMD Millipore Corporation | Mixed bed ion exchange adsorber |
| US20180086808A1 (en) * | 2015-03-16 | 2018-03-29 | Arven Ilac Sanayi Ve Ticaret A.S. | A process for preparing g-csf (granulocyte colony stimulating factor) |
| WO2017181920A1 (zh) * | 2016-04-18 | 2017-10-26 | 江苏恒瑞医药股份有限公司 | 一种重组人粒细胞集落刺激因子的制备方法 |
| KR20180064316A (ko) * | 2016-12-05 | 2018-06-14 | 한미약품 주식회사 | 재조합 단백질의 개선된 정제 방법 |
| KR102075256B1 (ko) * | 2018-07-16 | 2020-02-07 | 충북대학교 산학협력단 | 대식세포 콜로니 자극인자의 분리 및 정제 방법 |
| WO2020234742A1 (en) * | 2019-05-20 | 2020-11-26 | Lupin Limited | Granulocyte colony stimulating factor purification |
| CN115491328A (zh) * | 2022-08-31 | 2022-12-20 | 深圳市卫光生物制品股份有限公司 | 一种大肠杆菌表达蛋白及其表达纯化方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IE63992B1 (en) | 1985-09-17 | 1995-06-28 | Chugai Pharmaceutical Co Ltd | Human granulocyte colony stimulating factor |
| RU2057809C1 (ru) * | 1985-12-02 | 1996-04-10 | Чугай Сейяку Кабусики Кайся | Способ получения фактора, стимулирующего образование колоний гранулоцитов |
| JPH07165602A (ja) * | 1993-12-16 | 1995-06-27 | Kirin Brewery Co Ltd | 放射線障害防護剤 |
| KR100316347B1 (ko) | 1998-09-15 | 2002-08-27 | 한미약품(주) | 대장균엔테로톡신ⅱ신호펩티드의변형체와인체성장호르몬의융합단백질을발현하는재조합미생물및그를이용한인체성장호르몬의제조방법 |
| KR100356140B1 (ko) * | 1999-07-08 | 2002-10-19 | 한미약품공업 주식회사 | 인간 과립구 콜로니 자극인자 변이체 및 이의 생산 방법 |
| US6555660B2 (en) * | 2000-01-10 | 2003-04-29 | Maxygen Holdings Ltd. | G-CSF conjugates |
| EP1869078A2 (en) * | 2005-03-17 | 2007-12-26 | Zenotech Laboratories Limited | Process for the purification of recombinant granulocyte-colony stimulating factor |
| DE102005033250A1 (de) * | 2005-07-15 | 2007-01-18 | Bioceuticals Arzneimittel Ag | Verfahren zur Reinigung von G-CSF |
| GB0605684D0 (en) * | 2006-03-21 | 2006-05-03 | Sicor Biotech Uab | Method For Purifying Granulocyte-Colony Stimulating Factor |
-
2010
- 2010-10-29 KR KR1020100106994A patent/KR101831300B1/ko not_active Application Discontinuation
-
2011
- 2011-10-26 EP EP11836621.0A patent/EP2632944B1/en active Active
- 2011-10-26 CN CN201180056986.9A patent/CN103228672B/zh active Active
- 2011-10-26 JP JP2013536514A patent/JP6138690B2/ja active Active
- 2011-10-26 SG SG2013030804A patent/SG189509A1/en unknown
- 2011-10-26 AU AU2011321169A patent/AU2011321169B2/en active Active
- 2011-10-26 NZ NZ610314A patent/NZ610314A/en unknown
- 2011-10-26 CA CA2816050A patent/CA2816050C/en active Active
- 2011-10-26 DK DK11836621.0T patent/DK2632944T3/en active
- 2011-10-26 WO PCT/KR2011/008046 patent/WO2012057529A2/en active Application Filing
- 2011-10-26 ES ES11836621.0T patent/ES2640321T3/es active Active
- 2011-10-26 RU RU2013124809/10A patent/RU2596408C2/ru active
- 2011-10-26 US US13/881,637 patent/US9193761B2/en active Active
- 2011-10-26 BR BR112013010522-4A patent/BR112013010522B1/pt active IP Right Grant
- 2011-10-26 MX MX2013004830A patent/MX344815B/es active IP Right Grant
-
2013
- 2013-04-28 IL IL226043A patent/IL226043A/en active IP Right Grant
- 2013-05-27 ZA ZA2013/03834A patent/ZA201303834B/en unknown
-
2016
- 2016-11-07 JP JP2016216909A patent/JP2017060488A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| SG189509A1 (en) | 2013-05-31 |
| AU2011321169B2 (en) | 2016-02-25 |
| BR112013010522B1 (pt) | 2021-09-28 |
| IL226043A (en) | 2017-03-30 |
| DK2632944T3 (en) | 2017-10-02 |
| CN103228672B (zh) | 2015-09-16 |
| EP2632944B1 (en) | 2017-06-21 |
| WO2012057529A2 (en) | 2012-05-03 |
| CA2816050A1 (en) | 2012-05-03 |
| ZA201303834B (en) | 2014-04-30 |
| BR112013010522A2 (pt) | 2016-08-02 |
| KR101831300B1 (ko) | 2018-02-23 |
| EP2632944A4 (en) | 2015-07-08 |
| NZ610314A (en) | 2014-03-28 |
| KR20120045451A (ko) | 2012-05-09 |
| MX2013004830A (es) | 2013-06-03 |
| AU2011321169A1 (en) | 2013-06-06 |
| RU2596408C2 (ru) | 2016-09-10 |
| US9193761B2 (en) | 2015-11-24 |
| CN103228672A (zh) | 2013-07-31 |
| JP2017060488A (ja) | 2017-03-30 |
| JP2013540809A (ja) | 2013-11-07 |
| US20130211054A1 (en) | 2013-08-15 |
| EP2632944A2 (en) | 2013-09-04 |
| IL226043A0 (en) | 2013-06-27 |
| RU2013124809A (ru) | 2014-12-10 |
| ES2640321T3 (es) | 2017-11-02 |
| WO2012057529A3 (en) | 2012-06-28 |
| JP6138690B2 (ja) | 2017-05-31 |
| CA2816050C (en) | 2020-06-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |