MX342235B - Metodo optimizado para captura de anticuerpo por cromatografia de modo mixto. - Google Patents

Metodo optimizado para captura de anticuerpo por cromatografia de modo mixto.

Info

Publication number
MX342235B
MX342235B MX2013004627A MX2013004627A MX342235B MX 342235 B MX342235 B MX 342235B MX 2013004627 A MX2013004627 A MX 2013004627A MX 2013004627 A MX2013004627 A MX 2013004627A MX 342235 B MX342235 B MX 342235B
Authority
MX
Mexico
Prior art keywords
mixed mode
mode chromatography
optimized method
antibody capturing
protein
Prior art date
Application number
MX2013004627A
Other languages
English (en)
Other versions
MX2013004627A (es
Inventor
Roberto Falkenstein
Hidenari Yamada
Thorsten Lemm
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2013004627A publication Critical patent/MX2013004627A/es
Publication of MX342235B publication Critical patent/MX342235B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un método para producir un anticuerpo anti-IGF-1R, caracterizado porque comprende las siguientes etapas: a) aplicar un sobrenadante de cultivo de células de mamífero crudas a un material de cromatografía de intercambio de cationes débiles multimodal, b) recuperar el anticuerpo anti IGF-1R al aplicar una solución amortiguada que comprende etilen glicol y una sal inorgánica, al material de cromatografía de intercambio de cationes débiles multimodal y de esta manera producir un anticuerpo anti-IGF-1R.
MX2013004627A 2010-11-05 2011-11-02 Metodo optimizado para captura de anticuerpo por cromatografia de modo mixto. MX342235B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10190192 2010-11-05
PCT/EP2011/069202 WO2012059495A1 (en) 2010-11-05 2011-11-02 Optimized method for antibody capturing by mixed mode chromatography

Publications (2)

Publication Number Publication Date
MX2013004627A MX2013004627A (es) 2013-06-28
MX342235B true MX342235B (es) 2016-09-21

Family

ID=43768878

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013004627A MX342235B (es) 2010-11-05 2011-11-02 Metodo optimizado para captura de anticuerpo por cromatografia de modo mixto.

Country Status (10)

Country Link
US (3) US9422329B2 (es)
EP (1) EP2635600B1 (es)
JP (1) JP5947802B2 (es)
KR (1) KR101593766B1 (es)
CN (1) CN103201288B (es)
BR (1) BR112013007237A2 (es)
CA (1) CA2815282C (es)
MX (1) MX342235B (es)
RU (1) RU2591523C2 (es)
WO (1) WO2012059495A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2401040A (en) 2003-04-28 2004-11-03 Chugai Pharmaceutical Co Ltd Method for treating interleukin-6 related diseases
SI3202785T1 (sl) 2009-10-26 2024-08-30 F. Hoffmann-La Roche Ag Postopek za proizvodnjo glikoziliranega imunoglobulina
WO2012064627A2 (en) 2010-11-08 2012-05-18 Genentech, Inc. Subcutaneously administered anti-il-6 receptor antibody
GB201103363D0 (en) * 2011-02-28 2011-04-13 Ge Healthcare Uk Ltd Sample preservation method and sample application substrate
US9783570B2 (en) * 2011-07-01 2017-10-10 Hoffmann-La Roche Inc. Method for separation of monomeric polypeptides from aggregated polypeptides
SG11201407801VA (en) * 2012-05-31 2014-12-30 Agency Science Tech & Res Methods for use of mixed multifunctional surfaces for reducing aggregate content in protein preparations
CN105777862A (zh) * 2012-10-24 2016-07-20 建新公司 使用mes和mops作为流动相改性剂从多峰树脂洗脱生物分子
US10119944B2 (en) * 2013-12-24 2018-11-06 Waters Technologies Corporation Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides
US11092574B2 (en) 2013-12-24 2021-08-17 Waters Technologies Corporation Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides
CN105837687B (zh) * 2015-03-23 2019-08-16 广东东阳光药业有限公司 一种抗TNF-α类单克隆抗体的层析方法
MX2018015173A (es) * 2016-06-17 2019-07-04 Genentech Inc Purificacion de anticuerpos multiespecificos.
WO2018063981A1 (en) * 2016-09-29 2018-04-05 Bio-Rad Laboratories, Inc. Protein-nanoparticle conjugate purification methods

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2276672C2 (ru) * 2000-04-10 2006-05-20 Институт Биоорганической химии им. акад. М.М. Шемякина и Ю.А. Овчинникова Российской Академии Наук Антитело к фактору hldf, способ его получения (варианты), пептид с антигенными и нк-гидролизующими свойствами и способ диагностики анапластического состояния клетки человека
KR100788093B1 (ko) * 2002-04-26 2007-12-21 제넨테크, 인크. 단백질의 비친화성 정제
EP1613658B1 (en) 2003-04-02 2012-03-14 F. Hoffmann-La Roche AG Antibodies against insulin-like growth factor i receptor and uses thereof
DE102005050623A1 (de) 2005-10-21 2007-04-26 CCS Technology, Inc., Wilmington Optisches Kabel und Verfahren zur Herstellung eines optischen Kabels
CN101415728A (zh) * 2006-03-28 2009-04-22 比奥根艾迪克Ma公司 抗-igf-1r抗体及其用途
US20080014203A1 (en) 2006-04-11 2008-01-17 Silke Hansen Antibodies against insulin-like growth factor I receptor and uses thereof
US20080207487A1 (en) * 2006-11-02 2008-08-28 Neose Technologies, Inc. Manufacturing process for the production of polypeptides expressed in insect cell-lines
US7691980B2 (en) * 2007-01-09 2010-04-06 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
WO2009007993A2 (en) * 2007-04-23 2009-01-15 Arvind Mallinath Lali A process for purification of immunoglobulins using a pseudobioaffinity adsorbent
JP5485873B2 (ja) * 2007-05-22 2014-05-07 バクスター・インターナショナル・インコーポレイテッド ヒトフューリンのための予備的な精製方法
PL2167526T3 (pl) 2007-07-11 2011-09-30 Novo Nordisk As Oczyszczanie czynnika VIII za pomocą żywicy o mieszanej funkcji lub wielofunkcyjnej
EP2027875A1 (en) * 2007-08-23 2009-02-25 Octapharma AG A Process for Isolation and Purification of a Target Protein free of Prion Protein (PrPSC)
JP2011506396A (ja) 2007-12-13 2011-03-03 グラクソ グループ リミテッド 肺送達用組成物
NZ587511A (en) 2008-02-29 2012-08-31 Biogen Idec Inc Purified lymphotoxin-beta-receptor immunoglobulin fusion proteins and methods of their purification
JP5619738B2 (ja) * 2008-06-24 2014-11-05 オクタファルマ アクチェンゲゼルシャフト 凝固第ｖｉｉｉ因子の精製方法
CN102272144B (zh) * 2009-01-08 2014-09-17 通用电气健康护理生物科学股份公司 采用单一聚合物相系统的分离方法

Also Published As

Publication number Publication date
US10377794B2 (en) 2019-08-13
WO2012059495A1 (en) 2012-05-10
US9422329B2 (en) 2016-08-23
RU2591523C2 (ru) 2016-07-20
US20170015702A1 (en) 2017-01-19
RU2013123899A (ru) 2014-12-10
KR101593766B1 (ko) 2016-02-15
CA2815282A1 (en) 2012-05-10
KR20130067306A (ko) 2013-06-21
JP2014502259A (ja) 2014-01-30
CN103201288A (zh) 2013-07-10
MX2013004627A (es) 2013-06-28
EP2635600B1 (en) 2017-06-21
JP5947802B2 (ja) 2016-07-06
CA2815282C (en) 2019-05-21
BR112013007237A2 (pt) 2016-06-14
CN103201288B (zh) 2017-11-10
EP2635600A1 (en) 2013-09-11
US20130217866A1 (en) 2013-08-22
US20200165297A1 (en) 2020-05-28

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