MX2015005178A - Purificacion de polipeptidos usando ultrafiltracion de flujo tangencial de etapa doble. - Google Patents
Purificacion de polipeptidos usando ultrafiltracion de flujo tangencial de etapa doble.Info
- Publication number
- MX2015005178A MX2015005178A MX2015005178A MX2015005178A MX2015005178A MX 2015005178 A MX2015005178 A MX 2015005178A MX 2015005178 A MX2015005178 A MX 2015005178A MX 2015005178 A MX2015005178 A MX 2015005178A MX 2015005178 A MX2015005178 A MX 2015005178A
- Authority
- MX
- Mexico
- Prior art keywords
- processes
- unit operations
- purification
- dual stage
- methods
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/12—Purification
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2315/00—Details relating to the membrane module operation
- B01D2315/10—Cross-flow filtration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2317/00—Membrane module arrangements within a plant or an apparatus
- B01D2317/02—Elements in series
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/145—Ultrafiltration
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Water Supply & Treatment (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La presente invención está dirigida a métodos para la separación de una molécula de interés desde una solución que contiene la molécula utilizando la ultrafiltración de flujo tangencial de doble etapa ("TFF"). En particular, los métodos de la invención están dirigidos al procesamiento de las corrientes de alimentación crudas tales como el sobrenadante del cultivo celular acondicionado para reducir notablemente los niveles de los contaminantes y/o las impurezas previo a las operaciones unitarias de refinación subsiguientes, es decir corriente abajo. Los métodos de la invención pueden ser utilizados en el procesamiento de una corriente de alimentación cruda desde los sistemas de producción biológica tales como la fermentación u otro proceso de cultivo celular, y puede eliminar además la necesidad de los procesos de precipitación de las impurezas (por ejemplo, impulsado por el pH) y/o la filtración del precipitado, que consumen mucho tiempo, previo a los procesos corriente abajo que son sensibles a cargas de impurezas elevadas tales como las operaciones unitarias cromatográficas. El proceso de TFF de doble etapa descrito combina al menos dos operaciones unitarias de TFF que pueden ser llevadas a cabo ventajosamente a un pH que corresponde a, o que es aproximadamente aquel del pH de la corriente de alimentación, por ejemplo, un sobrenadante del cultivo celular, típicamente un pH de 7.5 - 1.0. El uso de las operaciones unitarias de TFF para suplementar, mejorar o reemplazar los procesos tradicionales para la purificación de las proteínas de interés para una corriente de alimentación puede representar ahorros significativos en los costos de procesamiento tanto directos como indirectos. Por ejemplo, además de los ahorros indirectos por la eliminación de los procesos de precipitación y de filtración del precipitado, la reducción en las cargas de las impurezas efectuadas por las operaciones unitarias de TFF de doble etapa puede conducir a ahorros indirectos por la mejora del funcionamiento de la columna corriente abajo, por ejemplo, de la separación cromatográfica, de la capacidad de aglutinación dinámica, del tiempo de vida útil opeativa y/o una reducción del tamaño de columna requerido. En las modalidades particulares, los métodos de la invención son utilizados en los procesos para la purificación de moléculas de inmunoglobulina, por ejemplos, los anticuerpos, tales procesos están desprovistos de etapas de purificación por afinidad, por ejemplo la purificación por cromatografía de afinidad con proteína A.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12190682 | 2012-10-30 | ||
| PCT/EP2013/072511 WO2014067898A1 (en) | 2012-10-30 | 2013-10-28 | Purification of polypeptides using dual stage tangential-flow ultrafiltration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2015005178A true MX2015005178A (es) | 2015-09-07 |
Family
ID=47115562
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2015005178A MX2015005178A (es) | 2012-10-30 | 2013-10-28 | Purificacion de polipeptidos usando ultrafiltracion de flujo tangencial de etapa doble. |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US20150274773A1 (es) |
| EP (1) | EP2914612B1 (es) |
| JP (1) | JP6345184B2 (es) |
| KR (1) | KR20150079830A (es) |
| CN (1) | CN104755489B (es) |
| BR (1) | BR112015009553A2 (es) |
| CA (1) | CA2887684A1 (es) |
| ES (1) | ES2706190T3 (es) |
| HK (1) | HK1208872A1 (es) |
| HR (1) | HRP20190043T1 (es) |
| MX (1) | MX2015005178A (es) |
| PL (1) | PL2914612T3 (es) |
| RU (1) | RU2632568C2 (es) |
| SI (1) | SI2914612T1 (es) |
| WO (1) | WO2014067898A1 (es) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2483305T3 (en) * | 2009-10-01 | 2016-10-24 | Hoffmann La Roche | MULTI-STEP-END FILTERING Immunoglobulin |
| US10550148B2 (en) * | 2014-06-16 | 2020-02-04 | Emd Millipore Corporation | Methods for increasing the capacity of flow-through processes |
| US10207225B2 (en) | 2014-06-16 | 2019-02-19 | Emd Millipore Corporation | Single-pass filtration systems and processes |
| EP2986361B1 (en) | 2014-06-25 | 2019-06-12 | EMD Millipore Corporation | Compact spiral-wound membrane filter elements |
| KR102061553B1 (ko) | 2014-08-29 | 2020-02-11 | 이엠디 밀리포어 코포레이션 | 잔류물의 재순환에 의한 싱글 패스 접선 유동 여과 시스템 및 접선 유동 여과 시스템을 사용하여 액체를 여과하는 공정 |
| CN105745009B (zh) | 2014-08-29 | 2018-09-28 | Emd 密理博公司 | 单程切向流过滤系统和具有渗余物的再循环的切向流过滤系统 |
| CN104845956A (zh) * | 2015-06-11 | 2015-08-19 | 山东农业大学 | 一种切向流超滤技术提取生姜蛋白酶的方法 |
| EP3443348A1 (en) * | 2016-04-14 | 2019-02-20 | Lonza Ltd | Compositions and methods for the detection of host cell proteins |
| TWI767930B (zh) * | 2016-08-16 | 2022-06-21 | 美商健臻公司 | 處理包括重組治療性蛋白之流體的方法及其用途 |
| EP3500588A1 (en) * | 2016-08-17 | 2019-06-26 | Boehringer Ingelheim International GmbH | Process for the preparation of highly concentrated liquid formulations containing biomolecules |
| AU2017347809A1 (en) * | 2016-10-28 | 2019-05-09 | Merck Sharp & Dohme Llc | Purification process for removal of tyrosine sulfation antibody variants; purified compositions |
| EP3141594A3 (en) * | 2016-11-11 | 2017-06-14 | Bayer Pharma Aktiengesellschaft | Method for sampling fluid streams for monitoring contaminants in a continuous flow |
| US20190359930A1 (en) * | 2016-11-11 | 2019-11-28 | Bayer Aktiengesellschaft | Method for sampling fluid streams for monitoring contaminants in a continuous flow |
| CN110241012B (zh) * | 2018-03-09 | 2022-11-01 | 嘉和生物药业有限公司 | 一种生物大分子上游分阶段截留的生产方法、生产模块及在生产中的应用 |
| US11028124B2 (en) * | 2018-05-07 | 2021-06-08 | Repligen Corporation | Methods, devices and systems for 3-stage filtration |
| WO2019236811A1 (en) * | 2018-06-08 | 2019-12-12 | Bristol-Myers Squibb Company | Single pass tangential flow filtration hybrid configurations for enhancing concentration of macromolecule solutions |
| CN114750976B (zh) * | 2022-06-15 | 2022-11-01 | 成都凯天电子股份有限公司 | 一种直升机自适应供油系统性能地面测试系统及方法 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4420398A (en) | 1981-08-13 | 1983-12-13 | American National Red Cross | Filteration method for cell produced antiviral substances |
| US4888115A (en) | 1983-12-29 | 1989-12-19 | Cuno, Incorporated | Cross-flow filtration |
| DE3432718C1 (de) | 1984-09-06 | 1986-05-22 | Biotest Pharma GmbH, 6000 Frankfurt | Verfahren zur Herstellung einer Loesung von Milch- und/oder Kolostralimmunglobulinen |
| GB2176715A (en) | 1985-06-27 | 1987-01-07 | Apv Int Ltd | Beer filtration |
| AT385426B (de) * | 1986-06-30 | 1988-03-25 | Vogelbusch Gmbh | Verfahren zum kontinuierlichen filtrieren einer fluessigkeit und einrichtung zur durchfuehrung des verfahrens |
| JPH03180192A (ja) * | 1989-12-07 | 1991-08-06 | Kirin Brewery Co Ltd | 遺伝子組換えによるヒトプロＴＧＦ―β１の製造 |
| US5256294A (en) | 1990-09-17 | 1993-10-26 | Genentech, Inc. | Tangential flow filtration process and apparatus |
| RU2049471C1 (ru) * | 1992-12-21 | 1995-12-10 | Научно-производственное объединение "Иммунопрепарат" | Способ получения гидролизата мозговой ткани "церебролизат" |
| DE19548630A1 (de) * | 1995-12-12 | 1997-06-19 | Schering Ag | Verfahren zur Reinigung von rekombinantem Human-Interleukin - 8 |
| US6214221B1 (en) * | 1999-02-22 | 2001-04-10 | Henry B. Kopf | Method and apparatus for purification of biological substances |
| US6492327B2 (en) * | 2000-12-19 | 2002-12-10 | Sulzer Biologics Inc. | Isolation of purified TGF- β1 and TGF -β2 from bone tissue |
| JP2006515227A (ja) * | 2003-02-24 | 2006-05-25 | ジーティーシー バイオセラピューティックス インコーポレイテッド | 十字流濾過の方法およびそのための装置 |
| US7531632B2 (en) * | 2006-02-16 | 2009-05-12 | Gtc Biotherapeutics, Inc. | Clarification of transgenic milk using depth filtration |
| US8741600B2 (en) | 2007-06-19 | 2014-06-03 | Asahi Kasei Kabushiki Kaisha | Method for separation of immunoglobulin monomers |
| US8268314B2 (en) | 2008-10-08 | 2012-09-18 | Hoffmann-La Roche Inc. | Bispecific anti-VEGF/anti-ANG-2 antibodies |
| CA2789071C (en) | 2010-03-05 | 2018-03-27 | F. Hoffmann-La Roche Ag | Antibodies against human csf-1r and uses thereof |
-
2013
- 2013-10-28 CN CN201380056755.7A patent/CN104755489B/zh active Active
- 2013-10-28 ES ES13794828T patent/ES2706190T3/es active Active
- 2013-10-28 JP JP2015538482A patent/JP6345184B2/ja active Active
- 2013-10-28 PL PL13794828T patent/PL2914612T3/pl unknown
- 2013-10-28 EP EP13794828.7A patent/EP2914612B1/en active Active
- 2013-10-28 SI SI201331326T patent/SI2914612T1/sl unknown
- 2013-10-28 BR BR112015009553A patent/BR112015009553A2/pt not_active IP Right Cessation
- 2013-10-28 KR KR1020157013846A patent/KR20150079830A/ko not_active Application Discontinuation
- 2013-10-28 WO PCT/EP2013/072511 patent/WO2014067898A1/en active Application Filing
- 2013-10-28 CA CA2887684A patent/CA2887684A1/en not_active Abandoned
- 2013-10-28 RU RU2015120458A patent/RU2632568C2/ru not_active IP Right Cessation
- 2013-10-28 MX MX2015005178A patent/MX2015005178A/es unknown
-
2015
- 2015-04-30 US US14/701,306 patent/US20150274773A1/en not_active Abandoned
- 2015-09-24 HK HK15109412.4A patent/HK1208872A1/xx unknown
-
2019
- 2019-01-04 HR HRP20190043TT patent/HRP20190043T1/hr unknown
-
2020
- 2020-02-21 US US16/798,141 patent/US20200291064A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20200291064A1 (en) | 2020-09-17 |
| WO2014067898A1 (en) | 2014-05-08 |
| JP2015533378A (ja) | 2015-11-24 |
| EP2914612B1 (en) | 2018-11-14 |
| CN104755489B (zh) | 2019-01-15 |
| SI2914612T1 (sl) | 2019-03-29 |
| CA2887684A1 (en) | 2014-05-08 |
| RU2632568C2 (ru) | 2017-10-05 |
| HK1208872A1 (en) | 2016-03-18 |
| US20150274773A1 (en) | 2015-10-01 |
| RU2015120458A (ru) | 2016-12-20 |
| BR112015009553A2 (pt) | 2017-10-03 |
| ES2706190T3 (es) | 2019-03-27 |
| EP2914612A1 (en) | 2015-09-09 |
| HRP20190043T1 (hr) | 2019-02-22 |
| CN104755489A (zh) | 2015-07-01 |
| KR20150079830A (ko) | 2015-07-08 |
| JP6345184B2 (ja) | 2018-06-20 |
| PL2914612T3 (pl) | 2019-04-30 |
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