SG11201407801VA - Methods for use of mixed multifunctional surfaces for reducing aggregate content in protein preparations - Google Patents

Methods for use of mixed multifunctional surfaces for reducing aggregate content in protein preparations

Info

Publication number
SG11201407801VA
SG11201407801VA SG11201407801VA SG11201407801VA SG11201407801VA SG 11201407801V A SG11201407801V A SG 11201407801VA SG 11201407801V A SG11201407801V A SG 11201407801VA SG 11201407801V A SG11201407801V A SG 11201407801VA SG 11201407801V A SG11201407801V A SG 11201407801VA
Authority
SG
Singapore
Prior art keywords
international
methods
singapore
protein preparations
pct
Prior art date
Application number
SG11201407801VA
Inventor
Peter Gagnon
Original Assignee
Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Publication of SG11201407801VA publication Critical patent/SG11201407801VA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Oncology (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 5 December 2013 (05.12.2013) WIPOIPCT (10) International Publication Number WO 2013/180648 A1 (51) International Patent Classification: C07K1/16 (2006.01) C07K16/00 (2006.01) C07K1/18 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/SG2013/000047 6 February 2013 (06.02.2013) English English (30) Priority Data: 61/653,904 31 May 2012 (31.05.2012) US (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH [SG/SG]; 1 Fusionpolis Way, #20-10 Connexis, Singapore 138632 (SG). (72) Inventor: GAGNON, Peter; 20 Biopolis Way, #06-01 Centres, Singapore 138668 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; P. O. Box 1531, Robinson Road Post Office, Singapore 903031 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3)) 00 •t o 00 i-H o CJ (54) Title: METHODS FOR USE OF MIXED MULTIFUNCTIONAL SURFACES FOR REDUCING AGGREGATE CONTENT IN PROTEIN PREPARATIONS (57) Abstract: Methods for reduction of aggregate levels in antibody and other protein preparations comprising the steps of treat­ ment with an organic multivalent cation (e.g., ethacridine, chlorhexidine, or polyethylenimine) and treatment with composition a having combination of a surfaces bearing electronegative chemical moieties and surfaces bearing electropositive chemical moieties.
SG11201407801VA 2012-05-31 2013-02-06 Methods for use of mixed multifunctional surfaces for reducing aggregate content in protein preparations SG11201407801VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261653904P 2012-05-31 2012-05-31
PCT/SG2013/000047 WO2013180648A1 (en) 2012-05-31 2013-02-06 Methods for use of mixed multifunctional surfaces for reducing aggregate content in protein preparations

Publications (1)

Publication Number Publication Date
SG11201407801VA true SG11201407801VA (en) 2014-12-30

Family

ID=49673719

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201407801VA SG11201407801VA (en) 2012-05-31 2013-02-06 Methods for use of mixed multifunctional surfaces for reducing aggregate content in protein preparations

Country Status (8)

Country Link
US (2) US9920125B2 (en)
EP (1) EP2855503B1 (en)
JP (1) JP6268167B2 (en)
KR (1) KR102065355B1 (en)
CN (1) CN104487446B (en)
IN (1) IN2014DN09955A (en)
SG (1) SG11201407801VA (en)
WO (1) WO2013180648A1 (en)

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EP2855502B1 (en) * 2012-05-31 2019-06-26 Agency For Science, Technology And Research Methods for reducing levels of protein-contaminant complexes and aggregates in protein preparations by treatment with electropositive organic additives
WO2014123483A1 (en) * 2013-02-06 2014-08-14 Agency For Science, Technology And Research Mixed multifunctional metal affinity surfaces for reducing aggregate content in protein preparations
KR20150115745A (en) * 2013-02-06 2015-10-14 에이전시 포 사이언스, 테크놀로지 앤드 리서치 Methods for reducing aggregate content in protein preparations
EP2961759A4 (en) * 2013-02-26 2016-09-28 Agency Science Tech & Res Protein purification in the presence of nonionic organic polymers and electropositive surfaces
US10253063B2 (en) 2013-02-28 2019-04-09 Agency For Science, Technology And Research Protein purification in the presence of nonionic organic polymers at elevated conductivity
CN105980846B (en) 2014-02-21 2018-12-07 宝生物工程美国有限公司 The method of column spinner and its manufacture and use including poly- (acid) UF membrane matrix
CN106061993A (en) * 2014-02-27 2016-10-26 新加坡科技研究局 Methods for reducing aggregate content of protein preparations by treatment with aryl anions
KR20170010750A (en) 2014-05-29 2017-02-01 에이전시 포 사이언스, 테크놀로지 앤드 리서치 Protein extraction methods
SG11201809685XA (en) 2016-06-15 2018-11-29 Agency Science Tech & Res A method of enhancing the performance of chromatography methods for purification of proteins
MX2020000353A (en) * 2017-07-10 2020-08-17 Takeda Pharmaceuticals Co Method for incubating liquids and inactivating viruses.
CN113166792A (en) * 2018-12-14 2021-07-23 比亚分离公司 A method for removing or eliminating endotoxin from an endotoxin-containing source or a potential endotoxin-containing source

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HU228076B1 (en) 1998-06-09 2012-10-29 Csl Behring Ag Process for producing immunoglobulins for intravenous administration and other immunoglobulin products
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US7691980B2 (en) * 2007-01-09 2010-04-06 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
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AU2009256308A1 (en) 2008-06-03 2009-12-10 Patrys Limited Process for purification of antibodies
US20100069617A1 (en) * 2008-09-12 2010-03-18 Ge Healthcare Bio-Sciences Ab Enhanced protein aggregate removal by mixed mode chromatography on hydrophobic interaction media in the presence of protein-excluded zwitterions
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Also Published As

Publication number Publication date
JP6268167B2 (en) 2018-01-24
KR20150033601A (en) 2015-04-01
EP2855503B1 (en) 2019-07-17
EP2855503A1 (en) 2015-04-08
US9920125B2 (en) 2018-03-20
KR102065355B1 (en) 2020-01-13
US20180201685A1 (en) 2018-07-19
US20150183879A1 (en) 2015-07-02
JP2015525213A (en) 2015-09-03
CN104487446A (en) 2015-04-01
CN104487446B (en) 2018-05-29
IN2014DN09955A (en) 2015-08-14
WO2013180648A1 (en) 2013-12-05
EP2855503A4 (en) 2015-12-16

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