BR112012007854A2 - metodos de produção de glicoproteínas em culturas de células de mamíferos usando glicocorticoides - Google Patents

metodos de produção de glicoproteínas em culturas de células de mamíferos usando glicocorticoides

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Publication number
BR112012007854A2
BR112012007854A2 BR112012007854A BR112012007854A BR112012007854A2 BR 112012007854 A2 BR112012007854 A2 BR 112012007854A2 BR 112012007854 A BR112012007854 A BR 112012007854A BR 112012007854 A BR112012007854 A BR 112012007854A BR 112012007854 A2 BR112012007854 A2 BR 112012007854A2
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BR
Brazil
Prior art keywords
mammalian cell
glucocorticoids
cell cultures
methods
production methods
Prior art date
Application number
BR112012007854A
Other languages
English (en)
Inventor
Ying Jing
Yueming Qian
Zheng-Jian Li
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Bristol Myers Squibb Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43823459&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112012007854(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Bristol Myers Squibb Co filed Critical Bristol Myers Squibb Co
Publication of BR112012007854A2 publication Critical patent/BR112012007854A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70521—CD28, CD152
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Reproductive Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

métodos de produção de glicoproteínas em culturaa de células de mamíferos usando glicocorticoides. a presente invenção refere-se a métodos e processos para a produção d eproteínas, particularmente glicopreoteínas, atarvés de cultura de célula de animal ou de mamífero, de preferência, mas não limitado a, culturas de célula alimentadas em bateladas. em um aspecto, os métodos compreendem a adição de composto glicocorticoide durante o período de cultura. a adição de composto glicocorticoide mantém uma alta biabilidade das células cultivadas, e pode render uma titulação de extremidade aumentada do produto de poretína, e uma alta qualidade do produto de proteína, como determinado, por eemplo, pelo conteúdo de áicdo siálico da proteína produzida.
BR112012007854A 2009-10-06 2010-10-06 metodos de produção de glicoproteínas em culturas de células de mamíferos usando glicocorticoides BR112012007854A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US27834309P 2009-10-06 2009-10-06
US12/897,857 US9540426B2 (en) 2009-10-06 2010-10-05 Mammalian cell culture processes for protein production
PCT/US2010/051552 WO2011044180A1 (en) 2009-10-06 2010-10-06 Methods of production of glycoproteins in mammalian cell cultures using glucocorticoids

Publications (1)

Publication Number Publication Date
BR112012007854A2 true BR112012007854A2 (pt) 2016-11-22

Family

ID=43823459

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012007854A BR112012007854A2 (pt) 2009-10-06 2010-10-06 metodos de produção de glicoproteínas em culturas de células de mamíferos usando glicocorticoides

Country Status (26)

Country Link
US (3) US9540426B2 (pt)
EP (1) EP2486048B2 (pt)
JP (1) JP2013506436A (pt)
KR (1) KR20120100973A (pt)
CN (1) CN102753572A (pt)
AR (1) AR078544A1 (pt)
AU (1) AU2010303590B2 (pt)
BR (1) BR112012007854A2 (pt)
CA (1) CA2777050C (pt)
CO (1) CO6531436A2 (pt)
CY (1) CY1118734T1 (pt)
DK (1) DK2486048T4 (pt)
EA (2) EA028491B1 (pt)
ES (1) ES2613269T5 (pt)
HR (1) HRP20170269T4 (pt)
HU (1) HUE031867T2 (pt)
IL (1) IL219124A (pt)
LT (1) LT2486048T (pt)
MX (1) MX2012003654A (pt)
PL (1) PL2486048T5 (pt)
PT (1) PT2486048T (pt)
RS (1) RS55727B2 (pt)
SI (2) SI2486048T1 (pt)
SM (1) SMT201700116B (pt)
TW (1) TW201118176A (pt)
WO (1) WO2011044180A1 (pt)

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Also Published As

Publication number Publication date
CN102753572A (zh) 2012-10-24
MX2012003654A (es) 2012-04-30
JP2013506436A (ja) 2013-02-28
ES2613269T3 (es) 2017-05-23
EA025604B1 (ru) 2017-01-30
PT2486048T (pt) 2017-02-06
US20110081679A1 (en) 2011-04-07
PL2486048T5 (pl) 2020-07-13
EP2486048B1 (en) 2016-11-23
EA201490631A1 (ru) 2016-05-31
US9540426B2 (en) 2017-01-10
HRP20170269T1 (hr) 2017-04-07
US10030064B2 (en) 2018-07-24
LT2486048T (lt) 2017-02-10
US20170129936A1 (en) 2017-05-11
HUE031867T2 (en) 2017-09-28
US10059754B2 (en) 2018-08-28
CA2777050A1 (en) 2011-04-14
DK2486048T3 (en) 2017-02-27
EA028491B1 (ru) 2017-11-30
EP2486048B2 (en) 2019-12-25
WO2011044180A1 (en) 2011-04-14
CY1118734T1 (el) 2017-07-12
AR078544A1 (es) 2011-11-16
CA2777050C (en) 2016-12-20
US20170129937A1 (en) 2017-05-11
SI2486048T2 (sl) 2020-04-30
SMT201700116B (it) 2017-03-08
KR20120100973A (ko) 2012-09-12
AU2010303590B2 (en) 2016-07-07
EP2486048A1 (en) 2012-08-15
IL219124A (en) 2017-10-31
RS55727B2 (sr) 2020-09-30
CO6531436A2 (es) 2012-09-28
DK2486048T4 (da) 2020-03-30
RS55727B1 (sr) 2017-07-31
PL2486048T3 (pl) 2017-07-31
ES2613269T5 (es) 2020-08-03
EA201270469A1 (ru) 2012-10-30
IL219124A0 (en) 2012-06-28
HRP20170269T4 (hr) 2020-04-03
AU2010303590A1 (en) 2012-05-03
TW201118176A (en) 2011-06-01
SI2486048T1 (sl) 2017-02-28

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