CO6531436A2 - Métodos de producción de glucoproteínas en cultivos de celulas de mamiferos usando glucocorticoides - Google Patents
Métodos de producción de glucoproteínas en cultivos de celulas de mamiferos usando glucocorticoidesInfo
- Publication number
- CO6531436A2 CO6531436A2 CO12058562A CO12058562A CO6531436A2 CO 6531436 A2 CO6531436 A2 CO 6531436A2 CO 12058562 A CO12058562 A CO 12058562A CO 12058562 A CO12058562 A CO 12058562A CO 6531436 A2 CO6531436 A2 CO 6531436A2
- Authority
- CO
- Colombia
- Prior art keywords
- production
- methods
- cells
- cultives
- glucoprotein
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70503—Immunoglobulin superfamily
- C07K14/70521—CD28, CD152
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2511/00—Cells for large scale production
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Reproductive Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
La presente invención describe métodos y procesos para la producción de proteínas, en particular de glucoproteínas, mediante el cultivo de células animales o de mamífero, preferentemente, pero no limitados a, cultivos celulares semicontinuos. En un aspecto, los métodos comprenden la adición de compuesto glucocorticoide durante el periodo de cultivo. La adición de compuesto glucocorticoide mantiene una viabilidad alta de los cultivos celulares y puede proporcionar un aumento en la titulación final de producto proteico y una calidad alta de producto proteico, tal como se determina, por ejemplo, por medio del contenido de ácido siálico en la proteína producida.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US27834309P | 2009-10-06 | 2009-10-06 | |
US12/897,857 US9540426B2 (en) | 2009-10-06 | 2010-10-05 | Mammalian cell culture processes for protein production |
Publications (1)
Publication Number | Publication Date |
---|---|
CO6531436A2 true CO6531436A2 (es) | 2012-09-28 |
Family
ID=43823459
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CO12058562A CO6531436A2 (es) | 2009-10-06 | 2012-04-10 | Métodos de producción de glucoproteínas en cultivos de celulas de mamiferos usando glucocorticoides |
Country Status (26)
Country | Link |
---|---|
US (3) | US9540426B2 (es) |
EP (1) | EP2486048B2 (es) |
JP (1) | JP2013506436A (es) |
KR (1) | KR20120100973A (es) |
CN (1) | CN102753572A (es) |
AR (1) | AR078544A1 (es) |
AU (1) | AU2010303590B2 (es) |
BR (1) | BR112012007854A2 (es) |
CA (1) | CA2777050C (es) |
CO (1) | CO6531436A2 (es) |
CY (1) | CY1118734T1 (es) |
DK (1) | DK2486048T4 (es) |
EA (2) | EA028491B1 (es) |
ES (1) | ES2613269T5 (es) |
HR (1) | HRP20170269T4 (es) |
HU (1) | HUE031867T2 (es) |
IL (1) | IL219124A (es) |
LT (1) | LT2486048T (es) |
MX (1) | MX2012003654A (es) |
PL (1) | PL2486048T5 (es) |
PT (1) | PT2486048T (es) |
RS (1) | RS55727B2 (es) |
SI (2) | SI2486048T2 (es) |
SM (1) | SMT201700116B (es) |
TW (1) | TW201118176A (es) |
WO (1) | WO2011044180A1 (es) |
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US9540426B2 (en) * | 2009-10-06 | 2017-01-10 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
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WO2013176754A1 (en) | 2012-05-24 | 2013-11-28 | Abbvie Inc. | Novel purification of antibodies using hydrophobic interaction chromatography |
US9926365B2 (en) * | 2012-06-29 | 2018-03-27 | Bristol-Myers Squibb Company | Methods for reducing glycoprotein aggregation |
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US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
JP6689189B2 (ja) | 2013-03-26 | 2020-04-28 | コヒラス・バイオサイエンシズ・インコーポレイテッド | タンパク質産生方法 |
EP3052640A2 (en) | 2013-10-04 | 2016-08-10 | AbbVie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
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US11124760B2 (en) * | 2014-03-24 | 2021-09-21 | Biogen Ma Inc. | Methods for overcoming glutamine deprivation during mammalian cell culture |
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JP7086601B2 (ja) * | 2014-10-21 | 2022-06-20 | ジェンノヴァ バイオファーマシューティカルズ リミテッド | 組換えtnk-tpa(テネクテプラーゼ)の単離及び商業生産のための新規精製方法 |
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US9540426B2 (en) * | 2009-10-06 | 2017-01-10 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
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2010
- 2010-10-05 US US12/897,857 patent/US9540426B2/en active Active
- 2010-10-06 PT PT107657140T patent/PT2486048T/pt unknown
- 2010-10-06 EA EA201490631A patent/EA028491B1/ru not_active IP Right Cessation
- 2010-10-06 CN CN2010800448022A patent/CN102753572A/zh active Pending
- 2010-10-06 LT LTEP10765714.0T patent/LT2486048T/lt unknown
- 2010-10-06 BR BR112012007854A patent/BR112012007854A2/pt not_active Application Discontinuation
- 2010-10-06 DK DK10765714.0T patent/DK2486048T4/da active
- 2010-10-06 EP EP10765714.0A patent/EP2486048B2/en active Active
- 2010-10-06 KR KR1020127011616A patent/KR20120100973A/ko not_active Application Discontinuation
- 2010-10-06 ES ES10765714T patent/ES2613269T5/es active Active
- 2010-10-06 SI SI201031364T patent/SI2486048T2/sl unknown
- 2010-10-06 MX MX2012003654A patent/MX2012003654A/es not_active Application Discontinuation
- 2010-10-06 TW TW099134097A patent/TW201118176A/zh unknown
- 2010-10-06 JP JP2012533259A patent/JP2013506436A/ja active Pending
- 2010-10-06 HU HUE10765714A patent/HUE031867T2/en unknown
- 2010-10-06 CA CA2777050A patent/CA2777050C/en active Active
- 2010-10-06 AR ARP100103638A patent/AR078544A1/es not_active Application Discontinuation
- 2010-10-06 RS RS20170199A patent/RS55727B2/sr unknown
- 2010-10-06 SI SI201031364A patent/SI2486048T1/sl unknown
- 2010-10-06 PL PL10765714T patent/PL2486048T5/pl unknown
- 2010-10-06 AU AU2010303590A patent/AU2010303590B2/en active Active
- 2010-10-06 WO PCT/US2010/051552 patent/WO2011044180A1/en active Application Filing
- 2010-10-06 EA EA201270469A patent/EA025604B1/ru not_active IP Right Cessation
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2012
- 2012-04-05 IL IL219124A patent/IL219124A/en active IP Right Grant
- 2012-04-10 CO CO12058562A patent/CO6531436A2/es unknown
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2016
- 2016-12-01 US US15/366,218 patent/US10059754B2/en active Active
- 2016-12-01 US US15/366,281 patent/US10030064B2/en active Active
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2017
- 2017-02-20 CY CY20171100223T patent/CY1118734T1/el unknown
- 2017-02-21 SM SM201700116T patent/SMT201700116B/it unknown
- 2017-02-21 HR HRP20170269TT patent/HRP20170269T4/hr unknown
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