AR078544A1 - Procedimiento de cultivo de celulas de mamiferos para la produccion de proteinas - Google Patents
Procedimiento de cultivo de celulas de mamiferos para la produccion de proteinasInfo
- Publication number
- AR078544A1 AR078544A1 ARP100103638A ARP100103638A AR078544A1 AR 078544 A1 AR078544 A1 AR 078544A1 AR P100103638 A ARP100103638 A AR P100103638A AR P100103638 A ARP100103638 A AR P100103638A AR 078544 A1 AR078544 A1 AR 078544A1
- Authority
- AR
- Argentina
- Prior art keywords
- proteins
- protein product
- production
- cultivation
- cell cultures
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70503—Immunoglobulin superfamily
- C07K14/70521—CD28, CD152
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2511/00—Cells for large scale production
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Reproductive Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Procedimientos y procesos para la produccion de proteínas, en particular de glucoproteínas, mediante el cultivo de células animales o de mamífero, preferentemente, pero no limitados a, cultivos celulares semicontinuos. En un aspecto, los procedimientos comprenden la adicion de compuesto glucocorticoide durante el periodo de cultivo. La adicion de compuesto glucocorticoide mantiene una viabilidad alta de los cultivos celulares y puede proporcionar un aumento en la titulacion final de producto proteico y una calidad alta de producto proteico, tal como se determina, por ejemplo, por medio del contenido de acido siálico en la proteína producida. La consistencia en la calidad del producto proteico, producido de manera recombinante, es un requisito para la aprobacion regulatoria del mismo para ser empleado como agente terapéutico y/o profiláctico.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US27834309P | 2009-10-06 | 2009-10-06 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR078544A1 true AR078544A1 (es) | 2011-11-16 |
Family
ID=43823459
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP100103638A AR078544A1 (es) | 2009-10-06 | 2010-10-06 | Procedimiento de cultivo de celulas de mamiferos para la produccion de proteinas |
Country Status (26)
Country | Link |
---|---|
US (3) | US9540426B2 (es) |
EP (1) | EP2486048B2 (es) |
JP (1) | JP2013506436A (es) |
KR (1) | KR20120100973A (es) |
CN (1) | CN102753572A (es) |
AR (1) | AR078544A1 (es) |
AU (1) | AU2010303590B2 (es) |
BR (1) | BR112012007854A2 (es) |
CA (1) | CA2777050C (es) |
CO (1) | CO6531436A2 (es) |
CY (1) | CY1118734T1 (es) |
DK (1) | DK2486048T4 (es) |
EA (2) | EA028491B1 (es) |
ES (1) | ES2613269T5 (es) |
HR (1) | HRP20170269T4 (es) |
HU (1) | HUE031867T2 (es) |
IL (1) | IL219124A (es) |
LT (1) | LT2486048T (es) |
MX (1) | MX2012003654A (es) |
PL (1) | PL2486048T5 (es) |
PT (1) | PT2486048T (es) |
RS (1) | RS55727B2 (es) |
SI (2) | SI2486048T1 (es) |
SM (1) | SMT201700116B (es) |
TW (1) | TW201118176A (es) |
WO (1) | WO2011044180A1 (es) |
Families Citing this family (40)
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US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
AU2009347206C1 (en) | 2008-10-20 | 2016-12-08 | Abbvie Inc. | Isolation and purification of antibodies using Protein A affinity chromatography |
CA2738499A1 (en) | 2008-10-20 | 2010-04-29 | Abbott Laboratories | Viral inactivation during purification of antibodies |
US9540426B2 (en) * | 2009-10-06 | 2017-01-10 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
US9556258B2 (en) | 2011-07-08 | 2017-01-31 | Merck Sharp & Dohme Corp. | Purification of fusion proteins |
US9150645B2 (en) | 2012-04-20 | 2015-10-06 | Abbvie, Inc. | Cell culture methods to reduce acidic species |
WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
US20130281355A1 (en) * | 2012-04-24 | 2013-10-24 | Genentech, Inc. | Cell culture compositions and methods for polypeptide production |
KR102292949B1 (ko) | 2012-05-02 | 2021-08-25 | 라이프 테크놀로지스 코포레이션 | 고밀도 성장 및 형질감염 배지 및 발현 인핸서의 독특한 페어링을 사용하는 포유동물 세포들 내의 고수율의 일시적 발현 |
US9249182B2 (en) | 2012-05-24 | 2016-02-02 | Abbvie, Inc. | Purification of antibodies using hydrophobic interaction chromatography |
US9926365B2 (en) * | 2012-06-29 | 2018-03-27 | Bristol-Myers Squibb Company | Methods for reducing glycoprotein aggregation |
US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
US9206390B2 (en) | 2012-09-02 | 2015-12-08 | Abbvie, Inc. | Methods to control protein heterogeneity |
EP2943563A1 (en) * | 2013-01-10 | 2015-11-18 | Biogen MA Inc. | Medium supplements for improved process performance |
CA2905010A1 (en) | 2013-03-12 | 2014-09-18 | Abbvie Inc. | Human antibodies that bind human tnf-alpha and methods of preparing the same |
WO2014151878A2 (en) | 2013-03-14 | 2014-09-25 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides |
US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
WO2014159579A1 (en) | 2013-03-14 | 2014-10-02 | Abbvie Inc. | MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE |
CA2907771C (en) | 2013-03-26 | 2023-01-10 | Coherus Biosciences, Inc. | Perfusion method for manufacturing etanercept |
EP3052640A2 (en) | 2013-10-04 | 2016-08-10 | AbbVie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
WO2015073884A2 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
EA202092845A1 (ru) * | 2014-01-30 | 2021-06-30 | Кохерус Байосайенсис, Инк. | Перфузионная среда |
US11124760B2 (en) * | 2014-03-24 | 2021-09-21 | Biogen Ma Inc. | Methods for overcoming glutamine deprivation during mammalian cell culture |
CN105296433B (zh) | 2014-08-01 | 2018-02-09 | 中山康方生物医药有限公司 | 一种ctla4抗体、其药物组合物及其用途 |
PT3209767T (pt) * | 2014-10-21 | 2020-11-09 | Gennova Biopharmaceuticals Ltd | Um novo processo de purificação para isolamento e produção comercial de tnk-tpa recombinante (tenecteplase) |
EP3283508B1 (en) | 2015-04-17 | 2021-03-17 | Alpine Immune Sciences, Inc. | Immunomodulatory proteins with tunable affinities |
CN106318998B (zh) * | 2015-07-08 | 2019-08-20 | 浙江海正博锐生物制药有限公司 | 用于提高重组人ⅱ型肿瘤坏死因子受体-抗体融合蛋白唾液酸化水平的组合物 |
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JP2018536404A (ja) * | 2015-11-09 | 2018-12-13 | ブリストル−マイヤーズ スクイブ カンパニーBristol−Myers Squibb Company | Cho細胞において産生したポリペプチドの品質特性を操作する方法 |
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KR20230020022A (ko) | 2017-10-10 | 2023-02-09 | 알파인 이뮨 사이언시즈, 인코포레이티드 | Ctla-4 변이체 면역조절 단백질 및 이의 용도 |
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KR101391457B1 (ko) * | 2005-12-20 | 2014-05-19 | 브리스톨-마이어스 스큅 컴퍼니 | 조성물 및 조성물의 제조 방법 |
US9540426B2 (en) * | 2009-10-06 | 2017-01-10 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
US8678606B2 (en) | 2010-06-14 | 2014-03-25 | Aja Berger | Carrying container with at least two light sources |
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2010
- 2010-10-05 US US12/897,857 patent/US9540426B2/en active Active
- 2010-10-06 AR ARP100103638A patent/AR078544A1/es not_active Application Discontinuation
- 2010-10-06 EP EP10765714.0A patent/EP2486048B2/en active Active
- 2010-10-06 WO PCT/US2010/051552 patent/WO2011044180A1/en active Application Filing
- 2010-10-06 JP JP2012533259A patent/JP2013506436A/ja active Pending
- 2010-10-06 TW TW099134097A patent/TW201118176A/zh unknown
- 2010-10-06 PT PT107657140T patent/PT2486048T/pt unknown
- 2010-10-06 HU HUE10765714A patent/HUE031867T2/en unknown
- 2010-10-06 MX MX2012003654A patent/MX2012003654A/es not_active Application Discontinuation
- 2010-10-06 EA EA201490631A patent/EA028491B1/ru not_active IP Right Cessation
- 2010-10-06 EA EA201270469A patent/EA025604B1/ru not_active IP Right Cessation
- 2010-10-06 RS RS20170199A patent/RS55727B2/sr unknown
- 2010-10-06 CA CA2777050A patent/CA2777050C/en active Active
- 2010-10-06 KR KR1020127011616A patent/KR20120100973A/ko not_active Application Discontinuation
- 2010-10-06 LT LTEP10765714.0T patent/LT2486048T/lt unknown
- 2010-10-06 PL PL10765714T patent/PL2486048T5/pl unknown
- 2010-10-06 DK DK10765714.0T patent/DK2486048T4/da active
- 2010-10-06 ES ES10765714T patent/ES2613269T5/es active Active
- 2010-10-06 CN CN2010800448022A patent/CN102753572A/zh active Pending
- 2010-10-06 SI SI201031364A patent/SI2486048T1/sl unknown
- 2010-10-06 AU AU2010303590A patent/AU2010303590B2/en active Active
- 2010-10-06 BR BR112012007854A patent/BR112012007854A2/pt not_active Application Discontinuation
- 2010-10-06 SI SI201031364T patent/SI2486048T2/sl unknown
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2012
- 2012-04-05 IL IL219124A patent/IL219124A/en active IP Right Grant
- 2012-04-10 CO CO12058562A patent/CO6531436A2/es unknown
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2016
- 2016-12-01 US US15/366,218 patent/US10059754B2/en active Active
- 2016-12-01 US US15/366,281 patent/US10030064B2/en active Active
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2017
- 2017-02-20 CY CY20171100223T patent/CY1118734T1/el unknown
- 2017-02-21 SM SM201700116T patent/SMT201700116B/it unknown
- 2017-02-21 HR HRP20170269TT patent/HRP20170269T4/hr unknown
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