BRPI1006628B8 - método para produzir uma enzima lipolítica - Google Patents

método para produzir uma enzima lipolítica

Info

Publication number
BRPI1006628B8
BRPI1006628B8 BRPI1006628A BRPI1006628A BRPI1006628B8 BR PI1006628 B8 BRPI1006628 B8 BR PI1006628B8 BR PI1006628 A BRPI1006628 A BR PI1006628A BR PI1006628 A BRPI1006628 A BR PI1006628A BR PI1006628 B8 BRPI1006628 B8 BR PI1006628B8
Authority
BR
Brazil
Prior art keywords
lipolytic enzyme
sequence
produce
producing
encoding
Prior art date
Application number
BRPI1006628A
Other languages
English (en)
Inventor
Lin Cherry
Faurschou Isaksen Mai
Rajabi Zargahi Masoud
Ward Michael
Hoegh Lorentsen Rikke
M Madrid Susan
Original Assignee
Danisco
Dupont Nutrition Biosci Aps
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40862772&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI1006628(B8) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Danisco, Dupont Nutrition Biosci Aps filed Critical Danisco
Publication of BRPI1006628A2 publication Critical patent/BRPI1006628A2/pt
Publication of BRPI1006628B1 publication Critical patent/BRPI1006628B1/pt
Publication of BRPI1006628B8 publication Critical patent/BRPI1006628B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/14—Yeasts or derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Food Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Animal Husbandry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

método para produzir uma enzima lipolítica a presente invenção fornece um método para produzir uma enzima lipolítica compreendendo as etapas de: (i) fornecer uma célula de trichoderma reesei que compreende pelo menos uma sequência de nucleotídeos heteróloga que codifica uma enzima lipolítica que compreende a sequência de aminoácidos exibida como a seq id no: 3 ou seq id no: 4; e (ii) cultivo das células em condições que permitam a expressão da(s) dita(s) sequência(s) de nucleotídeos heteróloga(s) que codifica(m) dita enzima lipolítica.
BRPI1006628A 2009-04-24 2010-04-23 método para produzir uma enzima lipolítica BRPI1006628B8 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US17227209P 2009-04-24 2009-04-24
US61/172,272 2009-04-24
GB0908770.1 2009-05-20
GBGB0908770.1A GB0908770D0 (en) 2009-04-24 2009-05-20 Method
PCT/IB2010/051802 WO2010122531A2 (en) 2009-04-24 2010-04-23 Method

Publications (3)

Publication Number Publication Date
BRPI1006628A2 BRPI1006628A2 (pt) 2015-08-25
BRPI1006628B1 BRPI1006628B1 (pt) 2019-04-16
BRPI1006628B8 true BRPI1006628B8 (pt) 2019-05-14

Family

ID=40862772

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI1006628A BRPI1006628B8 (pt) 2009-04-24 2010-04-23 método para produzir uma enzima lipolítica

Country Status (11)

Country Link
US (2) US8940519B2 (pt)
EP (1) EP2421963B2 (pt)
CN (1) CN102439143B (pt)
AU (1) AU2010240473B2 (pt)
BR (1) BRPI1006628B8 (pt)
CA (1) CA2759405C (pt)
DK (1) DK2421963T4 (pt)
ES (1) ES2616057T5 (pt)
GB (1) GB0908770D0 (pt)
NZ (1) NZ595833A (pt)
WO (1) WO2010122531A2 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0908770D0 (en) * 2009-04-24 2009-07-01 Danisco Method
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
MX352973B (es) 2011-09-22 2017-12-15 Danisco Us Inc Actividad de adnasa endogena para reducir el contenido de adn.
CN102604913B (zh) * 2012-04-05 2013-08-14 湖南尤特尔生化有限公司 一种疏棉状嗜热丝孢菌脂肪酶的制备方法和应用
AU2014300554B2 (en) * 2013-06-27 2017-10-26 Sasol Technology (Proprietary) Limited Production of biomass for use in the treatment of Fischer-Tropsch reaction water
CN106434597B (zh) * 2016-11-08 2019-10-01 湖南尤特尔生化有限公司 一种嗜热毛壳菌磷脂酶及其制备方法和应用

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
US3817837A (en) 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3939350A (en) 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4277437A (en) 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4366241A (en) 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4797361A (en) † 1983-10-24 1989-01-10 Lehigh University Microorganism and process
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP1186660A3 (fr) 1985-03-30 2002-03-20 KAUFFMAN, Stuart A. Procédé d'obtension d'ADN, ARN, peptides, polypeptides ou protéines, par une technique de recombination d'ADN
US5780292A (en) 1987-04-29 1998-07-14 Alko Group Ltd. Production of phytate degrading enzymes in trichoderma
WO1991017243A1 (en) 1990-05-09 1991-11-14 Novo Nordisk A/S A cellulase preparation comprising an endoglucanase enzyme
DE4112440C1 (pt) 1991-04-16 1992-10-22 Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De
DK0673429T3 (da) 1992-12-10 2002-10-07 Dsm Nv Fremstilling af heterologe proteiner i filamentøse svampe
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5741665A (en) 1994-05-10 1998-04-21 University Of Hawaii Light-regulated promoters for production of heterologous proteins in filamentous fungi
WO1998044804A1 (en) * 1997-04-09 1998-10-15 Danisco A/S Improved method for preparing flour doughs and products made from such doughs using glycerol oxidase
GB9821198D0 (en) 1998-09-30 1998-11-25 Danisco Enzyme
ATE323759T1 (de) † 1999-01-25 2006-05-15 Novozymes As Rückgewinnung eines proteins bei hohem ph-wert
AU4039400A (en) 1999-03-26 2000-10-16 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US7511005B2 (en) 2003-05-12 2009-03-31 Danisco Us Inc., Genencor Division Lipolytic enzyme elip
US20070213518A1 (en) * 2003-05-12 2007-09-13 Jones Brian E Novel Lipolytic Enzyme Lip2
EP1862539B1 (en) 2003-05-29 2012-01-25 Danisco US Inc. Novel trichoderma genes
EP2410048B1 (en) * 2005-01-24 2016-08-10 DSM IP Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
US7740307B2 (en) 2006-06-14 2010-06-22 Guido Benvenuto Motor vehicle component and methods for its manufacture
US20080026376A1 (en) 2006-07-11 2008-01-31 Huaming Wang KEX2 cleavage regions of recombinant fusion proteins
WO2008008950A2 (en) * 2006-07-14 2008-01-17 Novozymes, Inc. Methods for producing secreted polypeptides having biological activity
MX2009009692A (es) 2007-03-14 2010-03-03 Danisco Us Inc Genencor Div Alfa-amilasa de trichoderma reesei que es una enzima maltogenica.
EP2147104B1 (en) 2007-05-21 2015-09-09 Danisco US Inc. Use of an aspartic protease (nsp24) signal sequence for heterologous protein expression
US8450098B2 (en) 2007-05-21 2013-05-28 Danisco Us Inc. Method for introducing nucleic acids into fungal cells
FI120835B (fi) † 2007-07-10 2010-03-31 Valtion Teknillinen Uusia esteraaseja ja niiden käyttö
GB0908770D0 (en) * 2009-04-24 2009-07-01 Danisco Method

Also Published As

Publication number Publication date
CA2759405A1 (en) 2010-10-28
AU2010240473B2 (en) 2014-01-30
DK2421963T4 (da) 2023-03-27
CN102439143A (zh) 2012-05-02
AU2010240473A1 (en) 2011-11-10
WO2010122531A2 (en) 2010-10-28
CA2759405C (en) 2020-07-21
EP2421963A2 (en) 2012-02-29
BRPI1006628B1 (pt) 2019-04-16
NZ595833A (en) 2013-10-25
ES2616057T5 (es) 2023-05-23
BRPI1006628A2 (pt) 2015-08-25
EP2421963B2 (en) 2022-12-28
US20120196002A1 (en) 2012-08-02
EP2421963B1 (en) 2016-11-30
DK2421963T3 (en) 2017-03-06
ES2616057T3 (es) 2017-06-09
US9382523B2 (en) 2016-07-05
CN102439143B (zh) 2016-03-09
US8940519B2 (en) 2015-01-27
US20150064309A1 (en) 2015-03-05
WO2010122531A3 (en) 2010-12-29
GB0908770D0 (en) 2009-07-01

Similar Documents

Publication Publication Date Title
BRPI1006628B8 (pt) método para produzir uma enzima lipolítica
AR124871A2 (es) Métodos de fabricación para el control del contenido de lisina c-terminal, galactosa y ácido siálico en proteínas recombinantes
SG179048A1 (en) Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids
NZ608972A (en) Engineered nucleic acids and methods of use thereof
MY186219A (en) Production of proteins in glutamine-free cell culture media
IN2014DN10996A (pt)
IN2014CN01614A (pt)
WO2010089151A8 (en) Bioreactor for the cultivation of mammalian cells
BR112012015410A2 (pt) genes diacilglicerol aciltransferase e uso dos mesmos.
AR089873A1 (es) Vegetales resistentes al glifosato y metodos asociados
NZ630568A (en) Methods and means for the production of ch3 domain-comprising molecules
WO2010141801A3 (en) Reprogramming t cells and hematophietic cells
CO6531436A2 (es) Métodos de producción de glucoproteínas en cultivos de celulas de mamiferos usando glucocorticoides
BR112012033699A2 (pt) polipeptídeo tendo atividade beta-glicosidase e seus usos
IN2014CN01706A (pt)
BR112014026206A2 (pt) sistemas e métodos celulares para melhorar a síntese de ácidos graxos através da expressão da desidrogenase
AR083354A1 (es) Polipeptidos variantes cbh i (celobiohidrolasas i) con reducida inhibicion de producto
NZ725398A (en) Variants of chymosin with improved milk-clotting properties
NZ700897A (en) Lowering saturated fatty acid content of plant seeds
BR112013018744A2 (pt) método para produzir um polipetídeo ou uma proteína recombinante
BR112015005782A2 (pt) variantes de enzima com propriedades aprimoradas de éster-sintase
BR112016012229A2 (pt) métodos de produção de polipeptídeos
BR112012033714A2 (pt) polipeptídeo com atividade de acetil xilano esterase e seus usos.
BR112012015119A2 (pt) "ácidos graxos de cadeia ramificada e produção biológica dos mesmos".
FI20115328A (fi) Uusia kutinaaseja, niiden tuottaminen ja käytöt

Legal Events

Date Code Title Description
B25D Requested change of name of applicant approved

Owner name: DUPONT NUTRITION BIOSCIENCES APS (DK)

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B15K Others concerning applications: alteration of classification

Ipc: C12N 9/20 (2006.01), A23K 20/189 (2016.01), A23L 2

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 23/04/2010, OBSERVADAS AS CONDICOES LEGAIS. (CO) 20 (VINTE) ANOS CONTADOS A PARTIR DE 23/04/2010, OBSERVADAS AS CONDICOES LEGAIS

B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 23/04/2010, OBSERVADAS AS CONDICOES LEGAIS. (CO) REFERENTE A RPI 2519 DE 16/04/2019, QUANTO AO ENDERECO DA DEPOSITANTE E NOME DE UM INVENTOR.