ATE323759T1 - Rückgewinnung eines proteins bei hohem ph-wert - Google Patents

Rückgewinnung eines proteins bei hohem ph-wert

Info

Publication number
ATE323759T1
ATE323759T1 AT00901052T AT00901052T ATE323759T1 AT E323759 T1 ATE323759 T1 AT E323759T1 AT 00901052 T AT00901052 T AT 00901052T AT 00901052 T AT00901052 T AT 00901052T AT E323759 T1 ATE323759 T1 AT E323759T1
Authority
AT
Austria
Prior art keywords
protein
recovery
solution
state
glycosidase
Prior art date
Application number
AT00901052T
Other languages
English (en)
Inventor
Mads Aage Laustsen
Curran Matthew Simpson
Michael O'reilly
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE323759T1 publication Critical patent/ATE323759T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
AT00901052T 1999-01-25 2000-01-24 Rückgewinnung eines proteins bei hohem ph-wert ATE323759T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199900085 1999-01-25
DKPA199901491 1999-10-18

Publications (1)

Publication Number Publication Date
ATE323759T1 true ATE323759T1 (de) 2006-05-15

Family

ID=26063274

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00901052T ATE323759T1 (de) 1999-01-25 2000-01-24 Rückgewinnung eines proteins bei hohem ph-wert

Country Status (9)

Country Link
US (1) US6316240B1 (de)
EP (1) EP1149160B1 (de)
JP (1) JP2002534975A (de)
CN (1) CN1181197C (de)
AT (1) ATE323759T1 (de)
AU (1) AU2093100A (de)
DE (1) DE60027406T2 (de)
DK (1) DK1149160T3 (de)
WO (1) WO2000043502A1 (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7153818B2 (en) 2000-07-28 2006-12-26 Henkel Kgaa Amylolytic enzyme extracted from bacillus sp. A 7-7 (DSM 12368) and washing and cleaning agents containing this novel amylolytic enzyme
RU2325819C2 (ru) * 2006-03-16 2008-06-10 Вячеслав Юрьевич Елупов Йодированные крупы
CN101631797B (zh) * 2007-03-15 2015-12-16 诺维信公司 发酵液中蛋白酶晶体的溶解
RU2010154462A (ru) † 2008-06-09 2012-07-20 ДАНИСКО ЮЭс ИНК. (US) Выделение нерастворимого фермента из ферментного бульона и составление нерастворимого фермента
GB0908770D0 (en) 2009-04-24 2009-07-01 Danisco Method
US20150291656A1 (en) * 2012-11-01 2015-10-15 Novozymes A/S Method for removal of dna
US10259841B2 (en) * 2014-04-30 2019-04-16 Novozymes A/S Method for reducing the DNA content of a fermentation broth
KR20180085737A (ko) 2015-12-09 2018-07-27 바스프 에스이 탈착 조건 하에서 발효 고형분으로부터 단백질을 정제하는 방법
WO2018185048A1 (en) 2017-04-03 2018-10-11 Novozymes A/S Recovery process
CN112368375A (zh) 2018-04-26 2021-02-12 巴斯夫欧洲公司 脂肪酶
EP3788145A1 (de) 2018-05-03 2021-03-10 Basf Se Amylaseenzyme
MX2021010110A (es) 2019-02-20 2021-09-21 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de medio definido y alimentacion de magnesio.
WO2020169564A1 (en) 2019-02-20 2020-08-27 Basf Se Industrial fermentation process for bacillus using defined medium and trace element feed
WO2020193532A1 (en) 2019-03-25 2020-10-01 Basf Se Cleaning composition having amylase enzymes
WO2020193534A2 (en) 2019-03-25 2020-10-01 Basf Se Amylase enzymes
EP3947665A2 (de) 2019-03-25 2022-02-09 Basf Se Amylaseenzyme
US12146171B2 (en) 2019-06-13 2024-11-19 Basf Se Method of recovering a protein from fermentation broth using a divalent cation
EP3994273A1 (de) 2019-07-02 2022-05-11 Basf Se Verfahren zur herstellung eines fermentationsmediums
PL3959326T3 (pl) 2019-07-05 2023-10-09 Basf Se Przemysłowy sposób fermentacji dla komórek bakteryjnych z wykorzystaniem kultury wstępnej okresowej z zasilaniem
WO2021032881A1 (en) 2019-08-22 2021-02-25 Basf Se Amylase variants
JP2024508766A (ja) 2021-02-22 2024-02-28 ベーアーエスエフ・エスエー アミラーゼバリアント
EP4047088A1 (de) 2021-02-22 2022-08-24 Basf Se Amylasevarianten
EP4565710A1 (de) 2022-08-02 2025-06-11 Basf Se Verfahren zur herstellung von stabilisierten proteinen unter verwendung von bacillus-wirtszellen
CN119698472A (zh) 2022-08-11 2025-03-25 巴斯夫欧洲公司 包含蛋白酶、甘露聚糖酶和/或纤维素酶的酶组合物
CN119677846A (zh) 2022-08-11 2025-03-21 巴斯夫欧洲公司 淀粉酶变体
US20260055347A1 (en) 2022-08-11 2026-02-26 Basf Se Amylase variants
JP2025526759A (ja) 2022-08-11 2025-08-15 ビーエーエスエフ ソシエタス・ヨーロピア アミラーゼを含む酵素組成物
CN120693402A (zh) 2022-11-04 2025-09-23 巴斯夫欧洲公司 用于在洗涤剂组合物中使用的具有蛋白酶活性的多肽
WO2024094733A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2024094735A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
EP4389864A1 (de) 2022-12-20 2024-06-26 Basf Se Cutinasen
CN121666450A (zh) 2023-08-08 2026-03-13 巴斯夫欧洲公司 具有失活的金属蛋白酶的改良的芽孢杆菌细胞
WO2025202374A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202370A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202372A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
EP4624572A1 (de) 2024-03-27 2025-10-01 Basf Se Polypeptide mit proteaseaktivität zur verwendung in waschmittelzusammensetzungen
WO2025202369A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202379A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3373676D1 (en) * 1982-06-07 1987-10-22 Celltech Ltd A process for the preparation of chymosin
JPH06102034B2 (ja) 1983-03-25 1994-12-14 セルテク リミテツド タンパク質の生産方法
US5340926A (en) * 1983-03-25 1994-08-23 Celltech, Limited Process for the recovery of recombinantly produced protein from insoluble aggregate
CA1267615A (en) * 1984-08-27 1990-04-10 Dan Hadary Method for recovering purified growth hormones from genetically engineered microorganisms
JPH01222780A (ja) * 1988-03-02 1989-09-06 Tosoh Corp ウロキナーゼ前駆体様蛋白質のリフォールディング方法
US5064943A (en) * 1988-12-16 1991-11-12 American Cyanamid Company Method for solubilization and naturation of somatotropin
EP0574050A1 (de) 1992-05-19 1993-12-15 Gist-Brocades N.V. Trennung und Reinigung von Fermentationsprodukt in grossen Massstab
BE1009844A3 (fr) 1995-12-22 1997-10-07 Genencor Int Procede de recolte d'une proteine.

Also Published As

Publication number Publication date
EP1149160A1 (de) 2001-10-31
CN1337999A (zh) 2002-02-27
CN1181197C (zh) 2004-12-22
DK1149160T3 (da) 2006-08-14
DE60027406T2 (de) 2006-11-23
AU2093100A (en) 2000-08-07
EP1149160B1 (de) 2006-04-19
US6316240B1 (en) 2001-11-13
WO2000043502A1 (en) 2000-07-27
JP2002534975A (ja) 2002-10-22
DE60027406D1 (de) 2006-05-24

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