ATE323759T1 - Rückgewinnung eines proteins bei hohem ph-wert - Google Patents
Rückgewinnung eines proteins bei hohem ph-wertInfo
- Publication number
- ATE323759T1 ATE323759T1 AT00901052T AT00901052T ATE323759T1 AT E323759 T1 ATE323759 T1 AT E323759T1 AT 00901052 T AT00901052 T AT 00901052T AT 00901052 T AT00901052 T AT 00901052T AT E323759 T1 ATE323759 T1 AT E323759T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- recovery
- solution
- state
- glycosidase
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 238000011084 recovery Methods 0.000 title 1
- 102000005744 Glycoside Hydrolases Human genes 0.000 abstract 1
- 108010031186 Glycoside Hydrolases Proteins 0.000 abstract 1
- 102000035195 Peptidases Human genes 0.000 abstract 1
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 235000019833 protease Nutrition 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA199900085 | 1999-01-25 | ||
DKPA199901491 | 1999-10-18 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE323759T1 true ATE323759T1 (de) | 2006-05-15 |
Family
ID=26063274
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT00901052T ATE323759T1 (de) | 1999-01-25 | 2000-01-24 | Rückgewinnung eines proteins bei hohem ph-wert |
Country Status (9)
Country | Link |
---|---|
US (1) | US6316240B1 (de) |
EP (1) | EP1149160B1 (de) |
JP (1) | JP2002534975A (de) |
CN (1) | CN1181197C (de) |
AT (1) | ATE323759T1 (de) |
AU (1) | AU2093100A (de) |
DE (1) | DE60027406T2 (de) |
DK (1) | DK1149160T3 (de) |
WO (1) | WO2000043502A1 (de) |
Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5453116B2 (ja) * | 2007-03-15 | 2014-03-26 | ノボザイムス アクティーゼルスカブ | 発酵ブロス中のプロテアーゼ結晶の可溶化 |
EP2285823B2 (de) † | 2008-06-09 | 2024-03-06 | Danisco US Inc. | Rückgewinnung von unlöslichem enzym aus einer fermentationsbrühe und formulierung des unlöslichen enzyms |
GB0908770D0 (en) | 2009-04-24 | 2009-07-01 | Danisco | Method |
EP2914611B1 (de) * | 2012-11-01 | 2018-08-29 | Novozymes A/S | Verfahren zum entfernen von dna |
CN106255698B (zh) * | 2014-04-30 | 2021-08-03 | 诺维信公司 | 用于减少发酵液的dna含量的方法 |
JP2018536418A (ja) * | 2015-12-09 | 2018-12-13 | ビーエーエスエフ ソシエタス・ヨーロピアBasf Se | 脱離条件下で発酵固体からタンパク質を精製する方法 |
EP3606936B1 (de) | 2017-04-03 | 2021-10-20 | Novozymes A/S | Verfahren zur rückgewinnung |
CN112368375A (zh) | 2018-04-26 | 2021-02-12 | 巴斯夫欧洲公司 | 脂肪酶 |
WO2019211143A1 (en) | 2018-05-03 | 2019-11-07 | Basf Se | Amylase enzymes |
CN114127256A (zh) | 2019-02-20 | 2022-03-01 | 巴斯夫欧洲公司 | 用确定成分培养基和微量元素补料的芽孢杆菌工业发酵工艺 |
US20220186177A1 (en) | 2019-02-20 | 2022-06-16 | Basf Se | Industrial fermentation process for bacillus using defined medium and magnesium feed |
US20220170001A1 (en) | 2019-03-25 | 2022-06-02 | Basf Se | Amylase Enzymes |
WO2020193532A1 (en) | 2019-03-25 | 2020-10-01 | Basf Se | Cleaning composition having amylase enzymes |
US20220162576A1 (en) | 2019-03-25 | 2022-05-26 | Basf Se | Amylase enzymes |
US20220275355A1 (en) * | 2019-06-13 | 2022-09-01 | Basf Se | Method of Recovering a Protein from Fermentation Broth Using a Divalent Cation |
EP3994273A1 (de) | 2019-07-02 | 2022-05-11 | Basf Se | Verfahren zur herstellung eines fermentationsmediums |
CN114096677A (zh) | 2019-07-05 | 2022-02-25 | 巴斯夫欧洲公司 | 使用补料分批预培养的微生物细胞工业发酵方法 |
MX2022002182A (es) | 2019-08-22 | 2022-03-11 | Basf Se | Variantes de amilasa. |
KR20230147071A (ko) | 2021-02-22 | 2023-10-20 | 헨켈 아게 운트 코. 카게아아 | 아밀라제 변이체 |
EP4047088A1 (de) | 2021-02-22 | 2022-08-24 | Basf Se | Amylasevarianten |
WO2024028340A1 (en) | 2022-08-02 | 2024-02-08 | Basf Se | Stabilized protein production process using bacillus host cells with salt feed |
WO2024033133A2 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Enzyme compositions comprising an amylase |
WO2024033135A2 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Amylase variants |
WO2024033134A1 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Enzyme compositions comprising protease, mannanase, and/or cellulase |
WO2024033136A1 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Amylase variants |
WO2024094735A1 (en) | 2022-11-04 | 2024-05-10 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
WO2024094733A1 (en) | 2022-11-04 | 2024-05-10 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
WO2024094732A1 (en) | 2022-11-04 | 2024-05-10 | Basf Se | Polypeptides having protease activity for use in detergent compositions |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59500996A (ja) * | 1982-06-07 | 1984-06-07 | セルテク リミテツド | キモシンの生産方法 |
US5340926A (en) * | 1983-03-25 | 1994-08-23 | Celltech, Limited | Process for the recovery of recombinantly produced protein from insoluble aggregate |
DE122080T1 (de) * | 1983-03-25 | 1985-05-23 | Celltech Ltd., Slough, Berkshire | Verfahren zur herstellung eines proteins. |
CA1267615A (en) | 1984-08-27 | 1990-04-10 | Dan Hadary | Method for recovering purified growth hormones from genetically engineered microorganisms |
JPH01222780A (ja) | 1988-03-02 | 1989-09-06 | Tosoh Corp | ウロキナーゼ前駆体様蛋白質のリフォールディング方法 |
US5064943A (en) | 1988-12-16 | 1991-11-12 | American Cyanamid Company | Method for solubilization and naturation of somatotropin |
EP0574050A1 (de) | 1992-05-19 | 1993-12-15 | Gist-Brocades N.V. | Trennung und Reinigung von Fermentationsprodukt in grossen Massstab |
BE1009844A3 (fr) | 1995-12-22 | 1997-10-07 | Genencor Int | Procede de recolte d'une proteine. |
-
2000
- 2000-01-24 JP JP2000594910A patent/JP2002534975A/ja active Pending
- 2000-01-24 AU AU20931/00A patent/AU2093100A/en not_active Abandoned
- 2000-01-24 AT AT00901052T patent/ATE323759T1/de not_active IP Right Cessation
- 2000-01-24 EP EP00901052A patent/EP1149160B1/de not_active Expired - Lifetime
- 2000-01-24 DE DE60027406T patent/DE60027406T2/de not_active Expired - Lifetime
- 2000-01-24 US US09/489,712 patent/US6316240B1/en not_active Expired - Lifetime
- 2000-01-24 WO PCT/DK2000/000029 patent/WO2000043502A1/en active IP Right Grant
- 2000-01-24 DK DK00901052T patent/DK1149160T3/da active
- 2000-01-24 CN CNB008030650A patent/CN1181197C/zh not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE60027406T2 (de) | 2006-11-23 |
US6316240B1 (en) | 2001-11-13 |
EP1149160A1 (de) | 2001-10-31 |
EP1149160B1 (de) | 2006-04-19 |
DE60027406D1 (de) | 2006-05-24 |
AU2093100A (en) | 2000-08-07 |
JP2002534975A (ja) | 2002-10-22 |
CN1181197C (zh) | 2004-12-22 |
WO2000043502A1 (en) | 2000-07-27 |
DK1149160T3 (da) | 2006-08-14 |
CN1337999A (zh) | 2002-02-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ATE323759T1 (de) | Rückgewinnung eines proteins bei hohem ph-wert | |
ATE372334T1 (de) | Phenylpyrazolderivate als seh-inhibitoren | |
ATE326454T1 (de) | Neue bernsteinsäure derivative als cysteine- protease-inhibitoren | |
BR0012243A (pt) | Triflúor butenos nematocidas | |
ES2168300T3 (es) | Separacion de proteinas. | |
ATE450619T1 (de) | Defructosylierungsverfahren | |
TR199800011T1 (xx) | Benzo$g] kuinolin t�revleri | |
ATE274348T1 (de) | Überbrückte indenopyrrolocarbazole | |
ES2124400T3 (es) | Separacion de proteinas. | |
ATE474926T1 (de) | Verwendung von clya hemolysin fur ausscheidung von fusionprotein | |
DE69832268D1 (en) | Peptidyl-2-amino-1hydroxyalkansulfonsäuren als cystein-protease-inhibitoren | |
MY127603A (en) | Method to remove citrate and aluminum from proteins | |
DE69933855D1 (de) | Indolderivate zum nachweis von peptidasen von mikroorganismen | |
DK0840792T3 (da) | (S)-hydroxynitrillyase fra Hevea brasiliensis | |
DE69621837D1 (de) | Methoden zur feststellung von alzheimerscher krankheit | |
DE69710193T2 (de) | 6-Substituierte Amino-4-oxa-1-azabicyclo[3.2.0]heptan-7-on-Derivate als Cystein-Proteas-Inhibitoren | |
FI950094A0 (fi) | Menetelmä vesipohjaisen entsyymiliuoksen puhdistamiseksi | |
EA200400392A1 (ru) | Связывающий каспазу-8 белок, его получение и применение | |
DE60331414D1 (de) | Aggrecanase-1- und -2-peptidsubstrate und verfahren | |
DE69905772T2 (de) | Verfahren zur rückgewinnung von 3,4-epoxy-1-buten | |
ATE361320T1 (de) | Monoklonaler antikörper gegen protein c inhibitor | |
DE60015559D1 (de) | Sentrin-spezifische menschliche protease senp1 | |
ATE328106T1 (de) | Verfahren für die biotransformation von colchicon-verbindungen in die entsprechenden 3-glykosyl- derivate | |
DK0896579T3 (da) | Genindvindelse af proteiner ved præcipitation ved anvendelse af lignosulfater | |
DE59905355D1 (de) | Verwendung von katecholderivaten als proteinaseinhibitoren |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |