ATE323759T1 - Rückgewinnung eines proteins bei hohem ph-wert - Google Patents

Rückgewinnung eines proteins bei hohem ph-wert

Info

Publication number
ATE323759T1
ATE323759T1 AT00901052T AT00901052T ATE323759T1 AT E323759 T1 ATE323759 T1 AT E323759T1 AT 00901052 T AT00901052 T AT 00901052T AT 00901052 T AT00901052 T AT 00901052T AT E323759 T1 ATE323759 T1 AT E323759T1
Authority
AT
Austria
Prior art keywords
protein
recovery
solution
state
glycosidase
Prior art date
Application number
AT00901052T
Other languages
English (en)
Inventor
Mads Aage Laustsen
Curran Matthew Simpson
Michael O'reilly
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE323759T1 publication Critical patent/ATE323759T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
AT00901052T 1999-01-25 2000-01-24 Rückgewinnung eines proteins bei hohem ph-wert ATE323759T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199900085 1999-01-25
DKPA199901491 1999-10-18

Publications (1)

Publication Number Publication Date
ATE323759T1 true ATE323759T1 (de) 2006-05-15

Family

ID=26063274

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00901052T ATE323759T1 (de) 1999-01-25 2000-01-24 Rückgewinnung eines proteins bei hohem ph-wert

Country Status (9)

Country Link
US (1) US6316240B1 (de)
EP (1) EP1149160B1 (de)
JP (1) JP2002534975A (de)
CN (1) CN1181197C (de)
AT (1) ATE323759T1 (de)
AU (1) AU2093100A (de)
DE (1) DE60027406T2 (de)
DK (1) DK1149160T3 (de)
WO (1) WO2000043502A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5453116B2 (ja) * 2007-03-15 2014-03-26 ノボザイムス アクティーゼルスカブ 発酵ブロス中のプロテアーゼ結晶の可溶化
MX2010013179A (es) † 2008-06-09 2011-01-14 Danisco Inc Recuperacion de enzima insoluble a partir de caldo de fermentacion y formulacion de enzima insoluble.
GB0908770D0 (en) 2009-04-24 2009-07-01 Danisco Method
US20150291656A1 (en) * 2012-11-01 2015-10-15 Novozymes A/S Method for removal of dna
US10259841B2 (en) * 2014-04-30 2019-04-16 Novozymes A/S Method for reducing the DNA content of a fermentation broth
US20190024038A1 (en) 2015-12-09 2019-01-24 Basf Se Method of purifying a protein from fermentation solids under desorbing conditions
EP3606936B1 (de) 2017-04-03 2021-10-20 Novozymes A/S Verfahren zur rückgewinnung
EP3784779A1 (de) 2018-04-26 2021-03-03 Basf Se Lipaseenzyme
EP3788145A1 (de) 2018-05-03 2021-03-10 Basf Se Amylaseenzyme
CN114096676A (zh) 2019-02-20 2022-02-25 巴斯夫欧洲公司 用确定成分培养基和镁补料的芽孢杆菌工业发酵工艺
EP3927809A1 (de) 2019-02-20 2021-12-29 Basf Se Industrielles fermentationsverfahren für bacillus unter verwendung eines definierten mediums und spurenelementezufuhr
EP3947665A2 (de) 2019-03-25 2022-02-09 Basf Se Amylaseenzyme
WO2020193532A1 (en) 2019-03-25 2020-10-01 Basf Se Cleaning composition having amylase enzymes
EP3947664A2 (de) 2019-03-25 2022-02-09 Basf Se Amylaseenzyme
EP3983425A1 (de) * 2019-06-13 2022-04-20 Basf Se Verfahren zur rückgewinnung eines proteins aus fermentationsbrühe mithilfe eines divalenten kations
WO2021001297A1 (en) 2019-07-02 2021-01-07 Basf Se Method for preparing a fermentation medium
CN114096677A (zh) 2019-07-05 2022-02-25 巴斯夫欧洲公司 使用补料分批预培养的微生物细胞工业发酵方法
BR112022000573A2 (pt) 2019-08-22 2022-03-15 Basf Se Polipeptídeo isolado, sintético ou recombinante com atividade alfa-amilase, ácido nucleico isolado, sintético ou recombinante, construto de ácido nucleico, vetor de expressão, célula hospedeira, composição, método para produzir polipeptídeo isolado, sintético ou recombinante, método para preparar uma massa ou um produto de panificação, e, métodos para usar o polipeptídeo isolado, sintético ou recombinante e um domínio c de uma primeira amilase
KR20230147071A (ko) 2021-02-22 2023-10-20 헨켈 아게 운트 코. 카게아아 아밀라제 변이체
EP4047088A1 (de) 2021-02-22 2022-08-24 Basf Se Amylasevarianten
WO2024028340A1 (en) 2022-08-02 2024-02-08 Basf Se Stabilized protein production process using bacillus host cells with salt feed
WO2024033133A2 (en) 2022-08-11 2024-02-15 Basf Se Enzyme compositions comprising an amylase
WO2024033136A1 (en) 2022-08-11 2024-02-15 Basf Se Amylase variants
WO2024033134A1 (en) 2022-08-11 2024-02-15 Basf Se Enzyme compositions comprising protease, mannanase, and/or cellulase
WO2024033135A2 (en) 2022-08-11 2024-02-15 Basf Se Amylase variants

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3373676D1 (en) 1982-06-07 1987-10-22 Celltech Ltd A process for the preparation of chymosin
US5340926A (en) * 1983-03-25 1994-08-23 Celltech, Limited Process for the recovery of recombinantly produced protein from insoluble aggregate
GB2138004B (en) * 1983-03-25 1987-05-13 Celltech Ltd A process for the production of a protein
CA1267615A (en) 1984-08-27 1990-04-10 Dan Hadary Method for recovering purified growth hormones from genetically engineered microorganisms
JPH01222780A (ja) * 1988-03-02 1989-09-06 Tosoh Corp ウロキナーゼ前駆体様蛋白質のリフォールディング方法
US5064943A (en) 1988-12-16 1991-11-12 American Cyanamid Company Method for solubilization and naturation of somatotropin
EP0574050A1 (de) 1992-05-19 1993-12-15 Gist-Brocades N.V. Trennung und Reinigung von Fermentationsprodukt in grossen Massstab
BE1009844A3 (fr) 1995-12-22 1997-10-07 Genencor Int Procede de recolte d'une proteine.

Also Published As

Publication number Publication date
WO2000043502A1 (en) 2000-07-27
CN1337999A (zh) 2002-02-27
EP1149160B1 (de) 2006-04-19
US6316240B1 (en) 2001-11-13
JP2002534975A (ja) 2002-10-22
DK1149160T3 (da) 2006-08-14
DE60027406D1 (de) 2006-05-24
CN1181197C (zh) 2004-12-22
DE60027406T2 (de) 2006-11-23
EP1149160A1 (de) 2001-10-31
AU2093100A (en) 2000-08-07

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