BR112012015119A2 - "ácidos graxos de cadeia ramificada e produção biológica dos mesmos". - Google Patents
"ácidos graxos de cadeia ramificada e produção biológica dos mesmos".Info
- Publication number
- BR112012015119A2 BR112012015119A2 BR112012015119A BR112012015119A BR112012015119A2 BR 112012015119 A2 BR112012015119 A2 BR 112012015119A2 BR 112012015119 A BR112012015119 A BR 112012015119A BR 112012015119 A BR112012015119 A BR 112012015119A BR 112012015119 A2 BR112012015119 A2 BR 112012015119A2
- Authority
- BR
- Brazil
- Prior art keywords
- conversion
- fatty acid
- cell
- oxobutanoate
- chain fatty
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
- C12Y102/04—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a disulfide as acceptor (1.2.4)
- C12Y102/04004—3-Methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (1.2.4.4), i.e. branched-chain-alpha-ketoacid dehydrogenase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/0118—Beta-ketoacyl-acyl-carrier-protein synthase III (2.3.1.180)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01182—(R)-Citramalate synthase (2.3.1.182)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y403/00—Carbon-nitrogen lyases (4.3)
- C12Y403/01—Ammonia-lyases (4.3.1)
- C12Y403/01019—Threonine ammonia-lyase (4.3.1.19)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
"ácido graxos de cadeia ramificada e produção biológica dos mesmos" a presente invenção refere-se a um método para produzir ácido graxo de anteiso. o método compreende cultivar uma célula que compreende pelo menos um polinucleotídeo exógeno ou superexpresso que compreende uma sequência de ácido nucleico que codifica um polipeptídeo que catalisa pelo menos uma das seguintes reações: conversão de piruvato em citramalato; conversão de citramalato em citraconato; conversão de citraconato em b-metil-d-malato; conversão de b-metil-d-malato em 2-oxobutanoato; ou conversão de treonina em 2-oxobutanoato, em conndições que permitem a expressão do (s) polinucleotídeo (s) e produção de ácido graxo de anteiso. opcionalmente, a célula compreende adicionalmente pelo menos um polinucleotídeo exógeno ou superexpresso que compreende uma sequência de ácido nucleico que codifica um polipeptídeo que catalisa a conversão de 2-oxobutanoato em 2-aceto-2-hidróxi-butirato, a conversão de 2-aceto-2-hidróxi-butirato em 2,3-diidróxi-3-metilvalerato e/ou a conversão de 2,3-diidróxi-3-metivalerato em @-ceto-3-metilvalerato. uma célula que produz ácido graxo de anteiso e um método para o uso da célula para produzir ácido graxo de anteiso também são fornecidos.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US28903909P | 2009-12-22 | 2009-12-22 | |
US12/972,822 US20110151526A1 (en) | 2009-12-22 | 2010-12-20 | Branched-Chain Fatty Acids And Biological Production Thereof |
PCT/US2010/061544 WO2011087787A1 (en) | 2009-12-22 | 2010-12-21 | Branched-chain fatty acids and biological production thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112012015119A2 true BR112012015119A2 (pt) | 2017-03-28 |
Family
ID=44151653
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112012015119A BR112012015119A2 (pt) | 2009-12-22 | 2010-12-21 | "ácidos graxos de cadeia ramificada e produção biológica dos mesmos". |
Country Status (10)
Country | Link |
---|---|
US (1) | US20110151526A1 (pt) |
EP (1) | EP2516650B1 (pt) |
CN (1) | CN103097538B (pt) |
AU (1) | AU2010341495A1 (pt) |
BR (1) | BR112012015119A2 (pt) |
CA (1) | CA2781730A1 (pt) |
ES (1) | ES2525150T3 (pt) |
MX (1) | MX2012007184A (pt) |
WO (1) | WO2011087787A1 (pt) |
ZA (1) | ZA201203767B (pt) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8530221B2 (en) | 2010-01-14 | 2013-09-10 | Ls9, Inc. | Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells |
WO2012096686A1 (en) * | 2011-01-14 | 2012-07-19 | Ls9, Inc. | Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells |
WO2013152051A2 (en) | 2012-04-02 | 2013-10-10 | Ls9, Inc. | Improved production of fatty acid derivatives |
AR095504A1 (es) * | 2013-03-15 | 2015-10-21 | Consejo Nac De Investig Científicas Y Técnicas (Conicet) | Organismos obtenidos mediante ingeniería para la producción de lípidos |
KR101747542B1 (ko) * | 2015-10-23 | 2017-06-15 | 씨제이제일제당 (주) | L-이소루신 생산능을 가지는 코리네박테리움 속 미생물 및 이를 이용하여 l-이소루신을 생산하는 방법 |
WO2017165397A1 (en) | 2016-03-22 | 2017-09-28 | University Of Georgia Research Foundation, Inc. | Genetically engineered microbes and methods for producing citramalate |
CN109722405B (zh) * | 2017-10-31 | 2023-06-23 | 创享(天津)生物科技发展有限公司 | 利用葡萄糖生产异丙基吡喃酮的重组大肠杆菌及用途 |
JP2022513334A (ja) * | 2018-10-04 | 2022-02-07 | アンナ・ユニバーシティ | シトラマル酸、シトラコン酸又は2-オキソ酪酸からのl-2-アミノ酪酸の生成 |
CN110982742B (zh) * | 2019-12-12 | 2021-10-22 | 石河子大学 | 一种耐盐促生菌wp-3及其应用 |
CN118638831A (zh) * | 2024-08-13 | 2024-09-13 | 中国农业科学院北京畜牧兽医研究所 | 具有噬菌体抗性生产甲基苹果酸的大肠杆菌工程菌株、构建及发酵生产方法 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1358164A (zh) * | 1999-06-25 | 2002-07-10 | 考格尼斯德国有限责任公司 | 基本上不饱和的支链脂肪醇 |
EP2024491B1 (en) | 2006-05-19 | 2014-11-19 | LS9, Inc. | Production of fatty acids and derivatives thereof |
US8110670B2 (en) | 2006-05-19 | 2012-02-07 | Ls9, Inc. | Enhanced production of fatty acid derivatives |
US20100242345A1 (en) | 2006-05-19 | 2010-09-30 | LS9, Inc | Production of fatty acids & derivatives thereof |
US20090004715A1 (en) | 2007-06-01 | 2009-01-01 | Solazyme, Inc. | Glycerol Feedstock Utilization for Oil-Based Fuel Manufacturing |
CA3041892C (en) | 2008-10-28 | 2022-03-15 | REG Life Sciences, LLC | Methods for producing a fatty alcohol in a host cell |
US20100298612A1 (en) | 2009-05-22 | 2010-11-25 | Codexis, Inc. | Engineered biosynthesis of fatty alcohols |
-
2010
- 2010-12-20 US US12/972,822 patent/US20110151526A1/en not_active Abandoned
- 2010-12-21 BR BR112012015119A patent/BR112012015119A2/pt not_active IP Right Cessation
- 2010-12-21 AU AU2010341495A patent/AU2010341495A1/en not_active Abandoned
- 2010-12-21 ES ES10803687.2T patent/ES2525150T3/es active Active
- 2010-12-21 EP EP10803687.2A patent/EP2516650B1/en not_active Not-in-force
- 2010-12-21 WO PCT/US2010/061544 patent/WO2011087787A1/en active Application Filing
- 2010-12-21 CN CN201080058023.8A patent/CN103097538B/zh not_active Expired - Fee Related
- 2010-12-21 CA CA2781730A patent/CA2781730A1/en not_active Abandoned
- 2010-12-21 MX MX2012007184A patent/MX2012007184A/es active IP Right Grant
-
2012
- 2012-05-23 ZA ZA2012/03767A patent/ZA201203767B/en unknown
Also Published As
Publication number | Publication date |
---|---|
ES2525150T3 (es) | 2014-12-18 |
WO2011087787A1 (en) | 2011-07-21 |
ZA201203767B (en) | 2014-10-29 |
CN103097538A (zh) | 2013-05-08 |
EP2516650B1 (en) | 2014-09-17 |
CN103097538B (zh) | 2015-06-03 |
MX2012007184A (es) | 2012-07-10 |
CA2781730A1 (en) | 2011-07-21 |
US20110151526A1 (en) | 2011-06-23 |
EP2516650A1 (en) | 2012-10-31 |
AU2010341495A1 (en) | 2012-07-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] | ||
B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |