EA201270469A1 - Способы культивирования клеток млекопитающих для получения белка - Google Patents

Способы культивирования клеток млекопитающих для получения белка

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Publication number
EA201270469A1
EA201270469A1 EA201270469A EA201270469A EA201270469A1 EA 201270469 A1 EA201270469 A1 EA 201270469A1 EA 201270469 A EA201270469 A EA 201270469A EA 201270469 A EA201270469 A EA 201270469A EA 201270469 A1 EA201270469 A1 EA 201270469A1
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Prior art keywords
methods
mammalian cells
protein
cultivation
protein product
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EA201270469A
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English (en)
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EA025604B1 (ru
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Ин Цзин
Чжэнцзянь Ли
Юэмин Цянь
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Бристол-Майерс Сквибб Кампани
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43823459&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA201270469(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Бристол-Майерс Сквибб Кампани filed Critical Бристол-Майерс Сквибб Кампани
Publication of EA201270469A1 publication Critical patent/EA201270469A1/ru
Publication of EA025604B1 publication Critical patent/EA025604B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70521—CD28, CD152
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Reproductive Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Настоящее изобретение раскрывает методы и способы для получения белков, в частности гликопротеинов, с помощью культивирования клеток животных или клеток млекопитающих, предпочтительно, но не ограничиваясь ими, подпитываемые культуры клеток. В одном аспекте способы включают добавление глюкокортикоидного соединения в процессе периода культивирования. Добавление глюкокортикоидного соединения поддерживает высокую жизнеспособность культивируемых клеток и может приносить повышенный конечный титр продукта белка и высококачественный продукт белка, что определяют, например, содержанием сиаловой кислоты получаемого белка.
EA201270469A 2009-10-06 2010-10-06 СПОСОБ ПОЛУЧЕНИЯ ГЛИКОЗИЛИРОВАННОЙ РЕКОМБИНАНТНОЙ МОЛЕКУЛЫ CTLA4Ig EA025604B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US27834309P 2009-10-06 2009-10-06
US12/897,857 US9540426B2 (en) 2009-10-06 2010-10-05 Mammalian cell culture processes for protein production
PCT/US2010/051552 WO2011044180A1 (en) 2009-10-06 2010-10-06 Methods of production of glycoproteins in mammalian cell cultures using glucocorticoids

Publications (2)

Publication Number Publication Date
EA201270469A1 true EA201270469A1 (ru) 2012-10-30
EA025604B1 EA025604B1 (ru) 2017-01-30

Family

ID=43823459

Family Applications (2)

Application Number Title Priority Date Filing Date
EA201490631A EA028491B1 (ru) 2009-10-06 2010-10-06 СПОСОБ КУЛЬТИВИРОВАНИЯ КЛЕТОК ДЛЯ ПОЛУЧЕНИЯ ГЛИКОЗИЛИРОВАННОЙ РЕКОМБИНАНТНОЙ МОЛЕКУЛЫ CTLA4Ig
EA201270469A EA025604B1 (ru) 2009-10-06 2010-10-06 СПОСОБ ПОЛУЧЕНИЯ ГЛИКОЗИЛИРОВАННОЙ РЕКОМБИНАНТНОЙ МОЛЕКУЛЫ CTLA4Ig

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EA201490631A EA028491B1 (ru) 2009-10-06 2010-10-06 СПОСОБ КУЛЬТИВИРОВАНИЯ КЛЕТОК ДЛЯ ПОЛУЧЕНИЯ ГЛИКОЗИЛИРОВАННОЙ РЕКОМБИНАНТНОЙ МОЛЕКУЛЫ CTLA4Ig

Country Status (26)

Country Link
US (3) US9540426B2 (ru)
EP (1) EP2486048B2 (ru)
JP (1) JP2013506436A (ru)
KR (1) KR20120100973A (ru)
CN (1) CN102753572A (ru)
AR (1) AR078544A1 (ru)
AU (1) AU2010303590B2 (ru)
BR (1) BR112012007854A2 (ru)
CA (1) CA2777050C (ru)
CO (1) CO6531436A2 (ru)
CY (1) CY1118734T1 (ru)
DK (1) DK2486048T4 (ru)
EA (2) EA028491B1 (ru)
ES (1) ES2613269T5 (ru)
HR (1) HRP20170269T4 (ru)
HU (1) HUE031867T2 (ru)
IL (1) IL219124A (ru)
LT (1) LT2486048T (ru)
MX (1) MX2012003654A (ru)
PL (1) PL2486048T5 (ru)
PT (1) PT2486048T (ru)
RS (1) RS55727B2 (ru)
SI (2) SI2486048T1 (ru)
SM (1) SMT201700116B (ru)
TW (1) TW201118176A (ru)
WO (1) WO2011044180A1 (ru)

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US10030064B2 (en) 2018-07-24
TW201118176A (en) 2011-06-01
DK2486048T4 (da) 2020-03-30
US20170129937A1 (en) 2017-05-11
EP2486048A1 (en) 2012-08-15
CY1118734T1 (el) 2017-07-12
RS55727B1 (sr) 2017-07-31
HRP20170269T4 (hr) 2020-04-03
PL2486048T5 (pl) 2020-07-13
PT2486048T (pt) 2017-02-06
IL219124A (en) 2017-10-31
JP2013506436A (ja) 2013-02-28
PL2486048T3 (pl) 2017-07-31
MX2012003654A (es) 2012-04-30
EA028491B1 (ru) 2017-11-30
CA2777050C (en) 2016-12-20
CA2777050A1 (en) 2011-04-14
EA025604B1 (ru) 2017-01-30
SI2486048T2 (sl) 2020-04-30
CO6531436A2 (es) 2012-09-28
EA201490631A1 (ru) 2016-05-31
HUE031867T2 (en) 2017-09-28
EP2486048B2 (en) 2019-12-25
EP2486048B1 (en) 2016-11-23
WO2011044180A1 (en) 2011-04-14
AU2010303590B2 (en) 2016-07-07
LT2486048T (lt) 2017-02-10
DK2486048T3 (en) 2017-02-27
US20170129936A1 (en) 2017-05-11
RS55727B2 (sr) 2020-09-30
SI2486048T1 (sl) 2017-02-28
US9540426B2 (en) 2017-01-10
KR20120100973A (ko) 2012-09-12
SMT201700116B (it) 2017-03-08
US20110081679A1 (en) 2011-04-07
ES2613269T3 (es) 2017-05-23
HRP20170269T1 (hr) 2017-04-07
IL219124A0 (en) 2012-06-28
ES2613269T5 (es) 2020-08-03
CN102753572A (zh) 2012-10-24
AR078544A1 (es) 2011-11-16
AU2010303590A1 (en) 2012-05-03
BR112012007854A2 (pt) 2016-11-22
US10059754B2 (en) 2018-08-28

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