MX2013006234A - Metodo para la produccion de glicoproteinas recombinantes con semivida en circulacion incrementada en celulas de mamifero. - Google Patents

Metodo para la produccion de glicoproteinas recombinantes con semivida en circulacion incrementada en celulas de mamifero.

Info

Publication number
MX2013006234A
MX2013006234A MX2013006234A MX2013006234A MX2013006234A MX 2013006234 A MX2013006234 A MX 2013006234A MX 2013006234 A MX2013006234 A MX 2013006234A MX 2013006234 A MX2013006234 A MX 2013006234A MX 2013006234 A MX2013006234 A MX 2013006234A
Authority
MX
Mexico
Prior art keywords
life
production
circulatory half
recombinant glycoproteins
mammalian cells
Prior art date
Application number
MX2013006234A
Other languages
English (en)
Inventor
Michael J Betenbaugh
Original Assignee
Univ Johns Hopkins
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=46457991&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2013006234(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Johns Hopkins filed Critical Univ Johns Hopkins
Publication of MX2013006234A publication Critical patent/MX2013006234A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01214—Glycoprotein 3-alpha-L-fucosyltransferase (2.4.1.214)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99001—Beta-galactoside alpha-2,6-sialyltransferase (2.4.99.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01008—Cholinesterase (3.1.1.8), i.e. butyrylcholine-esterase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Se proporcionan en la presente métodos y sistemas de expresión recombinante para la producción de glicoproteínas recombinantes que tienen un contenido de ácido siálico aumentado y contienen predominantemente enlaces de ácido alfa 2-6 siálico. También se proporcionan en la presente glicoproteínas recombinantes que tienen una semivida en circulación in vivo aumentada. Una aplicación potencial de las glicoproteínas descritas en la presente es para el tratamiento y profilaxis de la intoxicación por neurotoxinas.
MX2013006234A 2011-01-06 2012-01-06 Metodo para la produccion de glicoproteinas recombinantes con semivida en circulacion incrementada en celulas de mamifero. MX2013006234A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161430256P 2011-01-06 2011-01-06
PCT/US2012/020535 WO2012094627A2 (en) 2011-01-06 2012-01-06 Method of production of recombinant glycoproteins with increased circulatory half-life in mammalian cells

Publications (1)

Publication Number Publication Date
MX2013006234A true MX2013006234A (es) 2013-08-01

Family

ID=46457991

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013006234A MX2013006234A (es) 2011-01-06 2012-01-06 Metodo para la produccion de glicoproteinas recombinantes con semivida en circulacion incrementada en celulas de mamifero.

Country Status (10)

Country Link
US (2) US20130243744A1 (es)
EP (1) EP2661492B1 (es)
CN (2) CN107267459A (es)
AU (1) AU2012204241A1 (es)
BR (1) BR112013016447A2 (es)
CA (1) CA2817765A1 (es)
DK (1) DK2661492T3 (es)
MX (1) MX2013006234A (es)
SG (1) SG190260A1 (es)
WO (1) WO2012094627A2 (es)

Families Citing this family (19)

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Publication number Priority date Publication date Assignee Title
US10130697B2 (en) 2010-03-23 2018-11-20 Wisconsin Alumni Research Foundation (Warf) Vaccines comprising mutant attenuated influenza viruses
CN104245932A (zh) 2012-01-11 2014-12-24 西格马-奥尔德里奇有限责任公司 具有简单糖型的重组蛋白的产生
EP3114222B1 (en) * 2014-03-04 2020-04-22 Sigma Aldrich Co. LLC Viral resistant cells and uses thereof
US10221402B2 (en) 2014-03-16 2019-03-05 Plantvax, Inc. Production of highly thermally stable recombinant cholinesterases for the detection, detoxification and decontamination of organophosphorus compounds
US10053671B2 (en) 2014-06-20 2018-08-21 Wisconsin Alumni Research Foundation (Warf) Mutations that confer genetic stability to additional genes in influenza viruses
HUE061672T2 (hu) 2014-11-12 2023-08-28 Seagen Inc Glikán-interakcióban lévõ vegyületek és felhasználási módszerek
SI3226888T1 (sl) 2014-12-01 2021-08-31 Ferring B.V. Dajanje selektivnega zaviralca signalizacije IL-6-trans
MA41116A (fr) 2014-12-01 2017-10-10 Ferring Bv Compositions d'inhibiteur de trans-signalisation par l'il-6 sélectif
WO2016091268A2 (en) * 2014-12-12 2016-06-16 University Of Copenhagen N-glycosylation
EP3042952A1 (en) * 2015-01-07 2016-07-13 CEVEC Pharmaceuticals GmbH O-glycan sialylated recombinant glycoproteins and cell lines for producing the same
JP7066613B2 (ja) 2015-11-12 2022-05-13 シージェン インコーポレイテッド グリカン相互作用化合物および使用方法
JP2019530672A (ja) * 2016-09-13 2019-10-24 バイエル・ヘルスケア・エルエルシーＢａｙｅｒ ＨｅａｌｔｈＣａｒｅＬＬＣ 第ｖｉｉａ因子グリコフォーム
WO2018094143A1 (en) 2016-11-17 2018-05-24 Siamab Therapeutics, Inc. Glycan-interacting compounds and methods of use
KR102653141B1 (ko) 2017-03-03 2024-04-01 씨젠 인크. 글리칸-상호작용 화합물 및 사용 방법
JP2020511499A (ja) * 2017-03-20 2020-04-16 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 赤血球生成刺激タンパク質のインビトロでの糖鎖改変のための方法
CN108659095A (zh) * 2018-05-18 2018-10-16 上海药明生物技术有限公司 一种使唾液酸含量稳定的方法
EP3921413A1 (en) 2019-02-08 2021-12-15 Wisconsin Alumni Research Foundation (WARF) Humanized cell line
US11807872B2 (en) 2019-08-27 2023-11-07 Wisconsin Alumni Research Foundation (Warf) Recombinant influenza viruses with stabilized HA for replication in eggs
CN117587020B (zh) * 2024-01-18 2024-04-02 中国人民解放军东部战区总医院 抑制ST3GAL1基因表达的shRNA及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6204431B1 (en) * 1994-03-09 2001-03-20 Abbott Laboratories Transgenic non-human mammals expressing heterologous glycosyltransferase DNA sequences produce oligosaccharides and glycoproteins in their milk
US20020045207A1 (en) * 1997-10-31 2002-04-18 Lynne A. Krummen Glycoprotein production process
WO2003048348A2 (en) * 2001-12-07 2003-06-12 Crucell Holland B.V. Production of viruses, viral isolates and vaccines
CN102180944A (zh) * 2001-10-10 2011-09-14 诺和诺德公司 肽的重构和糖缀合
AU2002365933A1 (en) * 2001-11-14 2003-09-02 Joseph D. Mosca Production of recombinant therapeutic bioscavengers against chemical and biological agents
US20070111279A1 (en) * 2002-11-13 2007-05-17 Procell, Inc. Production of recombinant therapeutic bioscavengers against chemical and biological agents
KR101667981B1 (ko) * 2006-12-28 2016-10-20 얀센 바이오테크 인코포레이티드 비시알릴화 면역글로불린의 생성 방법 및 생성용 벡터
EP2930245B1 (en) * 2007-10-12 2017-11-22 Sigma-Aldrich Co. LLC Cell line and methods for improved glycoprotein sialylation
AU2009338190C1 (en) * 2009-01-22 2014-07-17 Momenta Pharmaceuticals, Inc. Galactose-alpha-1, 3-galactose-containing N-glycans in glycoprotein products derived from CHO cells

Also Published As

Publication number Publication date
US20170159095A1 (en) 2017-06-08
CN107267459A (zh) 2017-10-20
AU2012204241A1 (en) 2013-06-06
EP2661492B1 (en) 2017-10-04
CA2817765A1 (en) 2012-07-12
BR112013016447A2 (pt) 2019-09-24
DK2661492T3 (en) 2018-01-15
WO2012094627A3 (en) 2012-10-04
EP2661492A4 (en) 2015-04-15
SG190260A1 (en) 2013-06-28
EP2661492A2 (en) 2013-11-13
CN103221537A (zh) 2013-07-24
WO2012094627A2 (en) 2012-07-12
US20130243744A1 (en) 2013-09-19

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