BR112013016447A2 - método de produção de glicoproteínas recombinantes com meia-vida circulatória aumentada em células de mamífero - Google Patents

método de produção de glicoproteínas recombinantes com meia-vida circulatória aumentada em células de mamífero

Info

Publication number
BR112013016447A2
BR112013016447A2 BR112013016447A BR112013016447A BR112013016447A2 BR 112013016447 A2 BR112013016447 A2 BR 112013016447A2 BR 112013016447 A BR112013016447 A BR 112013016447A BR 112013016447 A BR112013016447 A BR 112013016447A BR 112013016447 A2 BR112013016447 A2 BR 112013016447A2
Authority
BR
Brazil
Prior art keywords
life
circulatory half
mammalian cells
recombinant glycoproteins
production method
Prior art date
Application number
BR112013016447A
Other languages
English (en)
Inventor
J Betenbaugh Michael
Original Assignee
Univ Johns Hopkins
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=46457991&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112013016447(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Johns Hopkins filed Critical Univ Johns Hopkins
Publication of BR112013016447A2 publication Critical patent/BR112013016447A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01214—Glycoprotein 3-alpha-L-fucosyltransferase (2.4.1.214)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99001—Beta-galactoside alpha-2,6-sialyltransferase (2.4.99.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01008—Cholinesterase (3.1.1.8), i.e. butyrylcholine-esterase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

método de produção de glicoproteínas recombinantes com meia-vida circulatória aumentada em células de mamífero a presente invenção refere-se a métodos e sistemas de expressão recombinante para a produção de glicoproteínas recombinantes que têm teor de ácido siálico aumentado e contêm predominantemente ligações de ácido alfa2-6 siálio. também são fornecidas neste documento glicoproteínas recombinantes que têm uma meia-vida circulatória in vivo aumentada. uma aplicação potencial das glicoproteínas aqui descritas é para o tratamento e a profilaxia de envenenamento por neurotoxinas.
BR112013016447A 2011-01-06 2012-01-06 método de produção de glicoproteínas recombinantes com meia-vida circulatória aumentada em células de mamífero BR112013016447A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161430256P 2011-01-06 2011-01-06
PCT/US2012/020535 WO2012094627A2 (en) 2011-01-06 2012-01-06 Method of production of recombinant glycoproteins with increased circulatory half-life in mammalian cells

Publications (1)

Publication Number Publication Date
BR112013016447A2 true BR112013016447A2 (pt) 2019-09-24

Family

ID=46457991

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013016447A BR112013016447A2 (pt) 2011-01-06 2012-01-06 método de produção de glicoproteínas recombinantes com meia-vida circulatória aumentada em células de mamífero

Country Status (10)

Country Link
US (2) US20130243744A1 (pt)
EP (1) EP2661492B1 (pt)
CN (2) CN107267459A (pt)
AU (1) AU2012204241A1 (pt)
BR (1) BR112013016447A2 (pt)
CA (1) CA2817765A1 (pt)
DK (1) DK2661492T3 (pt)
MX (1) MX2013006234A (pt)
SG (1) SG190260A1 (pt)
WO (1) WO2012094627A2 (pt)

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KR102212865B1 (ko) 2012-01-11 2021-02-05 시그마-알드리치 컴퍼니., 엘엘씨 단순한 글리코형을 갖는 재조합 단백질의 생산
CN106661569B (zh) * 2014-03-04 2020-09-11 西格马—奥尔德里奇有限责任公司 抗病毒细胞及其用途
US10221402B2 (en) 2014-03-16 2019-03-05 Plantvax, Inc. Production of highly thermally stable recombinant cholinesterases for the detection, detoxification and decontamination of organophosphorus compounds
WO2015196150A2 (en) 2014-06-20 2015-12-23 Wisconsin Alumni Research Foundation (Warf) Mutations that confer genetic stability to additional genes in influenza viruses
DK3218005T3 (da) 2014-11-12 2023-03-27 Seagen Inc Glycan-interagerende forbindelser og anvendelsesfremgangsmåder
MA41116A (fr) * 2014-12-01 2017-10-10 Ferring Bv Compositions d'inhibiteur de trans-signalisation par l'il-6 sélectif
EP3912636A1 (en) 2014-12-01 2021-11-24 Ferring B.V. Administration of a selective il-6-trans-signalling inhibitor
WO2016091268A2 (en) * 2014-12-12 2016-06-16 University Of Copenhagen N-glycosylation
EP3042952A1 (en) * 2015-01-07 2016-07-13 CEVEC Pharmaceuticals GmbH O-glycan sialylated recombinant glycoproteins and cell lines for producing the same
KR20180088381A (ko) * 2015-11-12 2018-08-03 시아맙 쎄라퓨틱스, 인코포레이티드 글리칸-상호작용 화합물 및 사용방법
EP3512876B1 (en) * 2016-09-13 2021-10-27 Coagulant Therapeutics Corporation Factor viia glycoforms
EP3541847A4 (en) 2016-11-17 2020-07-08 Seattle Genetics, Inc. COMPOUNDS INTERACTING WITH GLYCANE AND METHODS OF USE
AU2018226824A1 (en) 2017-03-03 2019-09-19 Seagen Inc. Glycan-interacting compounds and methods of use
JP2020511499A (ja) * 2017-03-20 2020-04-16 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 赤血球生成刺激タンパク質のインビトロでの糖鎖改変のための方法
CN108659095A (zh) * 2018-05-18 2018-10-16 上海药明生物技术有限公司 一种使唾液酸含量稳定的方法
WO2020163804A1 (en) * 2019-02-08 2020-08-13 Wisconsin Alumni Research Foundation (Warf) Humanized cell line
JP2022551805A (ja) 2019-08-27 2022-12-14 ウィスコンシン アルムニ リサーチ ファンデイション 卵内複製のための安定化されたｈａを持つ組換えインフルエンザウイルス
CN117587020B (zh) * 2024-01-18 2024-04-02 中国人民解放军东部战区总医院 抑制ST3GAL1基因表达的shRNA及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6204431B1 (en) * 1994-03-09 2001-03-20 Abbott Laboratories Transgenic non-human mammals expressing heterologous glycosyltransferase DNA sequences produce oligosaccharides and glycoproteins in their milk
US20020045207A1 (en) * 1997-10-31 2002-04-18 Lynne A. Krummen Glycoprotein production process
DK1578771T3 (da) * 2001-10-10 2013-06-10 Novo Nordisk As Remodellering og glycokonjugering af peptider
AU2002365933A1 (en) * 2001-11-14 2003-09-02 Joseph D. Mosca Production of recombinant therapeutic bioscavengers against chemical and biological agents
PT1465987E (pt) * 2001-12-07 2008-04-15 Crucell Holland Bv Produção de vírus, isolados virais e vacinas
US20070111279A1 (en) * 2002-11-13 2007-05-17 Procell, Inc. Production of recombinant therapeutic bioscavengers against chemical and biological agents
BRPI0720861A2 (pt) * 2006-12-28 2014-02-25 Centocor Ortho Biotech Inc Métodos e vetores para gerar imunoglobulinas asialiladas
AU2008310583A1 (en) * 2007-10-12 2009-04-16 Sigma-Aldrich Co. Llc Compositions and methods for improved glycoprotein sialylation
BRPI0924078A2 (pt) * 2009-01-22 2018-10-16 Momenta Pharmaceuticals Inc n-glicanos contendo galactose-alfa-1,3-galactose em produtos de glicoproteína derivados de células de cho.

Also Published As

Publication number Publication date
EP2661492A4 (en) 2015-04-15
CN107267459A (zh) 2017-10-20
MX2013006234A (es) 2013-08-01
EP2661492B1 (en) 2017-10-04
CA2817765A1 (en) 2012-07-12
US20130243744A1 (en) 2013-09-19
DK2661492T3 (en) 2018-01-15
US20170159095A1 (en) 2017-06-08
CN103221537A (zh) 2013-07-24
AU2012204241A1 (en) 2013-06-06
WO2012094627A2 (en) 2012-07-12
WO2012094627A3 (en) 2012-10-04
SG190260A1 (en) 2013-06-28
EP2661492A2 (en) 2013-11-13

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2543 DE 01-10-2019 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.