BR112015010544A2 - processamento de lotes descontínuos alimentados com o uso de biorreatores alternados - Google Patents

processamento de lotes descontínuos alimentados com o uso de biorreatores alternados

Info

Publication number
BR112015010544A2
BR112015010544A2 BR112015010544A BR112015010544A BR112015010544A2 BR 112015010544 A2 BR112015010544 A2 BR 112015010544A2 BR 112015010544 A BR112015010544 A BR 112015010544A BR 112015010544 A BR112015010544 A BR 112015010544A BR 112015010544 A2 BR112015010544 A2 BR 112015010544A2
Authority
BR
Brazil
Prior art keywords
bioreactors
batch
fed
discontinuous
alternate
Prior art date
Application number
BR112015010544A
Other languages
English (en)
Other versions
BR112015010544B1 (pt
Inventor
Arthur Tijsterman Jacob
Original Assignee
Biosana Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biosana Pty Ltd filed Critical Biosana Pty Ltd
Publication of BR112015010544A2 publication Critical patent/BR112015010544A2/pt
Publication of BR112015010544B1 publication Critical patent/BR112015010544B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

discontinuous fed batch processing with the use of alternating bioreactors abstract the present invention relates to discontinuous fed batch cell culturing of mammalian cells (more specific cho cells) with the use of alternating bioreactors in combination with discontinuous separation of biomass from biological substance (more specific monoclonal antibodies) and waste components. tradução do resumo resumo patente de invenção: "processamento de lotes descontínuos alimentados com o uso de biorreatores alternados". a presente invenção refere-se à cultura de célula de lotes descontínuos alimentados de células mamíferas (mais especificamente, células de cho) com o uso de biorreatores alternados em combinação com a separação descontínua de biomassa da substância biológica (mais especificamente, anticorpos monoclonais) e componentes residuais. 1/1
BR112015010544-0A 2012-11-09 2013-11-08 método e sistema para a produção de uma substância biológica BR112015010544B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
NL2012050798 2012-11-09
NLPCT/NL2012/050798 2012-11-09
PCT/NL2013/050805 WO2014073967A1 (en) 2012-11-09 2013-11-08 Discontinuous fed batch processing with the use of alternating bioreactors

Publications (2)

Publication Number Publication Date
BR112015010544A2 true BR112015010544A2 (pt) 2017-07-11
BR112015010544B1 BR112015010544B1 (pt) 2021-01-05

Family

ID=47278493

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015010544-0A BR112015010544B1 (pt) 2012-11-09 2013-11-08 método e sistema para a produção de uma substância biológica

Country Status (11)

Country Link
US (1) US9982226B2 (pt)
EP (1) EP2917323A1 (pt)
AU (1) AU2013341858B2 (pt)
BR (1) BR112015010544B1 (pt)
CA (1) CA2890813C (pt)
MX (1) MX364790B (pt)
MY (1) MY181264A (pt)
RU (1) RU2639556C2 (pt)
SG (2) SG10201703385YA (pt)
TW (1) TWI637057B (pt)
WO (1) WO2014073967A1 (pt)

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CA2730528C (en) * 2008-07-16 2018-06-12 Kbi Biopharma, Inc. Methods and systems for manipulating particles using a fluidized bed
CN105637085A (zh) 2013-10-11 2016-06-01 瑞泽恩制药公司 代谢优化的细胞培养物
US11390663B2 (en) 2013-10-11 2022-07-19 Regeneron Pharmaceuticals, Inc. Metabolically optimized cell culture
SG10202113019XA (en) 2014-06-04 2021-12-30 Amgen Inc Methods for harvesting mammalian cell cultures
CN109689191B (zh) * 2016-07-25 2021-11-19 瑞普利金公司 交替切向流快速收获
US11920119B2 (en) 2018-02-09 2024-03-05 Global Life Sciences Solutions Usa Llc Systems and methods for bioprocessing
US11414639B2 (en) 2018-02-09 2022-08-16 Global Life Sciences Solutions Usa Llc Bioprocessing vessel
US11932842B2 (en) 2018-02-09 2024-03-19 Global Life Sciences Solutions Usa Llc Bioprocessing apparatus
US11371007B2 (en) * 2018-02-09 2022-06-28 Global Life Sciences Solutions Usa Llc System and method for fluid flow management in a bioprocessing system
US10792618B2 (en) 2018-06-19 2020-10-06 Sartorius Stedim Biotech Gmbh Particle separation and/or purification of a fluid
TWI662122B (zh) * 2018-07-12 2019-06-11 聯發生物科技股份有限公司 發酵設備
GB201904083D0 (en) * 2019-03-25 2019-05-08 Ge Healthcare Bio Sciences Ab A bioreactor system
AU2021315939A1 (en) * 2020-07-28 2023-02-23 Seagen Inc. Methods and systems for producing polypeptides
EP4056671A1 (en) * 2021-03-10 2022-09-14 Sartorius Stedim Biotech GmbH Method for operating a bioprocess installation
EP4124653A1 (en) * 2021-07-27 2023-02-01 Sartorius Stedim Biotech GmbH Method for producing a bioproduct
WO2023167584A1 (en) * 2022-03-01 2023-09-07 Biosanapharma B.V. Continuous manufacturing of a biological substance with process traceability

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK309184D0 (da) * 1984-06-25 1984-06-25 Nordisk Insulinlab Fremgangsmaade til isolering af insulin eller insulinlignende materiale fra en fermenteringsvaeske
DE3833925A1 (de) * 1988-03-11 1989-09-21 Inst Angewandte Biotechnologie Verfahren und herstellung von virus und viralem antigen und vorrichtung hierzu
RU2058992C1 (ru) * 1993-07-14 1996-04-27 Антипин Георгий Васильевич Способ получения кормового белка и устройство для его осуществления
WO2005007269A1 (en) 2003-06-17 2005-01-27 Centocor, Inc. Method and apparatus for filtration of bioreactor recombinant proteins
WO2005035748A1 (en) * 2003-10-10 2005-04-21 Novo Nordisk Health Care Ag Method for large-scale production of a polypeptide in eukaryote cells and a culture vessel suitable therefor
AU2005229359C1 (en) 2004-03-05 2011-07-28 Patheon Holdings I B.V. Process for cell culturing by continuous perfusion and alternating tangential flow
ES2579957T3 (es) 2006-07-14 2016-08-17 Dpx Holdings B.V. Procedimiento mejorado para el cultivo de células
EP3327132A3 (en) * 2007-08-09 2018-07-18 Wyeth LLC Use of perfusion to enhance production of fed-batch cell culture in bioreactors
JP5329644B2 (ja) * 2008-04-22 2013-10-30 ニューマチック スケール コーポレイション 単回使用のコンポーネントを備える遠心分離システム
CA2730528C (en) * 2008-07-16 2018-06-12 Kbi Biopharma, Inc. Methods and systems for manipulating particles using a fluidized bed
JP5774012B2 (ja) 2009-10-06 2015-09-02 ケーセップ・システムズ，リミテッド・ライアビリティ・カンパニー 粒子を処理するための方法、システム、および装置
RS56484B1 (sr) * 2009-11-17 2018-01-31 Squibb & Sons Llc Metode za poboljšanu proizvodnju proteina
US20130309757A1 (en) * 2010-12-17 2013-11-21 Sung-Chun Kim Method and apparatus for producing cells and fat soluble materials by cell culture

Also Published As

Publication number Publication date
RU2639556C2 (ru) 2017-12-21
SG11201601362VA (en) 2016-04-28
CA2890813A1 (en) 2014-05-15
BR112015010544B1 (pt) 2021-01-05
US20150299644A1 (en) 2015-10-22
MX2015005822A (es) 2016-02-10
RU2016105100A (ru) 2017-08-21
MX364790B (es) 2019-05-03
AU2013341858A1 (en) 2015-06-11
SG10201703385YA (en) 2017-05-30
AU2013341858B2 (en) 2017-11-02
TW201418468A (zh) 2014-05-16
US9982226B2 (en) 2018-05-29
EP2917323A1 (en) 2015-09-16
WO2014073967A1 (en) 2014-05-15
CA2890813C (en) 2022-07-12
MY181264A (en) 2020-12-21
TWI637057B (zh) 2018-10-01

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B06T Formal requirements before examination [chapter 6.20 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 08/11/2013, OBSERVADAS AS CONDICOES LEGAIS.