ATE481483T1 - Verfahren zur herstellung eines rekombinanten polyklonalen proteins - Google Patents

Verfahren zur herstellung eines rekombinanten polyklonalen proteins

Info

Publication number
ATE481483T1
ATE481483T1 AT08748815T AT08748815T ATE481483T1 AT E481483 T1 ATE481483 T1 AT E481483T1 AT 08748815 T AT08748815 T AT 08748815T AT 08748815 T AT08748815 T AT 08748815T AT E481483 T1 ATE481483 T1 AT E481483T1
Authority
AT
Austria
Prior art keywords
collection
cells
recombinant polyclonal
polyclonal protein
producing
Prior art date
Application number
AT08748815T
Other languages
English (en)
Inventor
Lars Nielsen
Dietmar Weilguny
Anne Tolstrup
Finn Wiberg
Christian Mueller
Original Assignee
Symphogen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Symphogen As filed Critical Symphogen As
Application granted granted Critical
Publication of ATE481483T1 publication Critical patent/ATE481483T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081—DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Non-Volatile Memory (AREA)
AT08748815T 2007-05-25 2008-05-21 Verfahren zur herstellung eines rekombinanten polyklonalen proteins ATE481483T1 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200700764 2007-05-25
US92470807P 2007-05-29 2007-05-29
DKPA200701292 2007-09-07
US96000207P 2007-09-11 2007-09-11
PCT/DK2008/050116 WO2008145133A2 (en) 2007-05-25 2008-05-21 Method for manufacturing a recombinant polyclonal protein

Publications (1)

Publication Number Publication Date
ATE481483T1 true ATE481483T1 (de) 2010-10-15

Family

ID=39739965

Family Applications (1)

Application Number Title Priority Date Filing Date
AT08748815T ATE481483T1 (de) 2007-05-25 2008-05-21 Verfahren zur herstellung eines rekombinanten polyklonalen proteins

Country Status (22)

Country Link
US (1) US7910332B2 (de)
EP (1) EP2152872B1 (de)
JP (2) JP5941616B2 (de)
KR (1) KR101555658B1 (de)
CN (1) CN101688204B (de)
AT (1) ATE481483T1 (de)
AU (1) AU2008255383B2 (de)
BR (1) BRPI0812091A2 (de)
CA (1) CA2683800C (de)
CY (1) CY1110939T1 (de)
DE (1) DE602008002593D1 (de)
DK (1) DK2152872T3 (de)
HR (1) HRP20100640T1 (de)
IL (1) IL201395A (de)
MX (1) MX2009012526A (de)
NZ (1) NZ581316A (de)
PL (1) PL2152872T3 (de)
PT (1) PT2152872E (de)
SI (1) SI2152872T1 (de)
TW (1) TW200902548A (de)
WO (1) WO2008145133A2 (de)
ZA (1) ZA200908021B (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA05006724A (es) 2003-01-07 2005-09-08 Symphogen As Metodo para producir proteinas policlonales recombinantes.
CA2677771A1 (en) * 2007-03-01 2008-09-04 Symphogen A/S Method for cloning cognate antibodies
PL2152872T3 (pl) * 2007-05-25 2011-03-31 Symphogen As Sposób wytwarzania rekombinowanego białka poliklonalnego
EP2185590A2 (de) * 2007-09-07 2010-05-19 Symphogen A/S Verfahren zur rekombinanten herstellung von anti-rsv-antikörpern
WO2009102569A2 (en) * 2008-02-01 2009-08-20 Chromocell Corporation Novel cell lines and methods
EP2098536A1 (de) 2008-03-05 2009-09-09 4-Antibody AG Isolation und Identifikation antigen- oder ligandenspezifischer Bindungsproteine
KR20110016899A (ko) * 2008-04-23 2011-02-18 심포젠 에이/에스 폴리클로날 단백질을 제조하는 방법
WO2009155008A1 (en) * 2008-05-28 2009-12-23 Bayer Healthcare Llc Methods and compositions for production of recombinant protein in hbx-expressing mammalian cells
WO2010022738A1 (en) * 2008-08-29 2010-03-04 Symphogen A/S Method for cloning avian-derived antibodies
DK2393927T3 (en) 2009-02-09 2015-03-30 Morphosys Ag Preparation of oligoclonal mixtures of antibodies in the individual cells
EP3156421B1 (de) 2010-11-01 2018-06-06 Symphogen A/S Pan-her-antikörperzusammensetzung
EP2640743B1 (de) 2010-11-16 2016-10-05 Excelimmune, Inc. Verfahren zur herstellung rekombinanter proteine
CN102558353A (zh) * 2011-12-09 2012-07-11 上海应用技术学院 一种大肠杆菌NrfA基因表达蛋白多克隆抗体及其制备方法
GB2502127A (en) 2012-05-17 2013-11-20 Kymab Ltd Multivalent antibodies and in vivo methods for their production
MX358728B (es) 2012-05-02 2018-09-03 Symphogen As Composiciones de anticuerpos pan-receptor del factor de crecimiento epidermico humano (pan-her) humanizados.
EP2953970A4 (de) 2013-02-08 2016-06-29 Misfolding Diagnostics Inc Transthyretinantikörper und verwendungen davon
CN107001471B (zh) 2014-09-16 2022-01-18 西福根有限公司 抗met抗体和组合物
US12404552B2 (en) * 2015-07-17 2025-09-02 Allele Biotechnology & Pharmaceuticals, Inc. Methods of selecting antibodies and antibody fragments
WO2017121896A1 (en) * 2016-01-14 2017-07-20 Anton Bauer Method of producing a production cell line
WO2018073393A2 (en) 2016-10-19 2018-04-26 Cellectis Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy
CA3185303A1 (en) 2017-04-05 2018-10-11 Symphogen A/S Combination therapies targeting pd-a, tim-3, and lag-3
CA3104390A1 (en) * 2018-06-22 2019-12-26 Genmab A/S Method for producing a controlled mixture of two or more different antibodies
WO2020084034A1 (en) 2018-10-26 2020-04-30 F. Hoffmann-La Roche Ag Multispecific antibody screening method using recombinase mediated cassette exchange
CA3118692A1 (en) 2018-11-06 2020-05-14 Alsatech, Inc. Cell-based gene therapy for neurodegenerative diseases
CN109988751A (zh) * 2019-01-15 2019-07-09 济宁学院 一种用于制备抗egfr全人源化单克隆抗体的细胞株构建方法
EP4283295A3 (de) * 2019-04-30 2024-03-06 Gigagen, Inc. Rekombinante polyklonale proteine und verfahren zur verwendung davon
JP2022550083A (ja) * 2019-09-27 2022-11-30 グラクソスミスクライン、インテレクチュアル、プロパティー、ディベロップメント、リミテッド クローン細胞株の生産安定性を予測する方法
WO2021253002A1 (en) 2020-06-12 2021-12-16 Gigagen, Inc. Recombinant polyclonal proteins targeting covid-19 and methods of use thereof
EP4713360A1 (de) 2023-05-19 2026-03-25 Les Laboratoires Servier Anti-met-antikörper, antikörper-wirkstoff-konjugate, zusammensetzungen und verwendungen davon

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016074A1 (en) 1990-04-19 1991-10-31 Medimmune, Inc. Administration of monoclonal antibodies against respiratory viruses
GB9022543D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Antibody production
US6111166A (en) 1994-09-19 2000-08-29 Medarex, Incorporated Transgenic mice expressing human Fcα and β receptors
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
EP1293564A4 (de) * 2000-06-08 2005-08-17 Mitsubishi Pharma Corp Expressionsvektor zum screenen von zellen mit hoher expression von rekombinanten proteinen, transformand mit hoher expression eines rekombinanten proteins und dessen verwendung
US6849259B2 (en) 2000-06-16 2005-02-01 Symphogen A/S Polyclonal antibody composition for treating allergy
PT2314629E (pt) * 2002-07-18 2014-01-22 Merus B V Produção recombinante de misturas de anticorpos
US20040115814A1 (en) * 2002-09-30 2004-06-17 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
CN100383244C (zh) * 2003-01-07 2008-04-23 西福根有限公司 用于生产重组多克隆蛋白质的方法
US20060275766A1 (en) 2003-01-07 2006-12-07 Haurum John S Method for manufacturing recombinant polyclonal proteins
MXPA05006724A (es) * 2003-01-07 2005-09-08 Symphogen As Metodo para producir proteinas policlonales recombinantes.
TWI333977B (en) 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
KR20070038556A (ko) * 2004-07-20 2007-04-10 심포젠 에이/에스 항-ｒｈｅｓｕｓ ｄ 재조합 폴리클로날 항체 및 이의 제조방법
AU2005263334C1 (en) * 2004-07-20 2011-01-20 Symphogen A/S A procedure for structural characterization of a recombinant polyclonal protein or a polyclonal cell line
US7850965B2 (en) 2005-12-05 2010-12-14 Symphogen A/S Anti-orthopoxvirus recombinant polyclonal antibody
CA2638833A1 (en) 2006-03-06 2007-09-13 Symphogen A/S Recombinant polyclonal antibody for treatment of respiratory syncytial virus infections
PL2152872T3 (pl) * 2007-05-25 2011-03-31 Symphogen As Sposób wytwarzania rekombinowanego białka poliklonalnego

Also Published As

Publication number Publication date
CN101688204A (zh) 2010-03-31
JP5941616B2 (ja) 2016-06-29
TW200902548A (en) 2009-01-16
US7910332B2 (en) 2011-03-22
DK2152872T3 (da) 2010-12-06
WO2008145133A3 (en) 2009-02-05
NZ581316A (en) 2012-02-24
IL201395A0 (en) 2011-08-01
KR101555658B1 (ko) 2015-09-24
DE602008002593D1 (de) 2010-10-28
WO2008145133A2 (en) 2008-12-04
BRPI0812091A2 (pt) 2014-10-14
CY1110939T1 (el) 2015-06-10
KR20100017923A (ko) 2010-02-16
HK1134519A1 (en) 2010-04-30
CN101688204B (zh) 2013-07-03
EP2152872A2 (de) 2010-02-17
MX2009012526A (es) 2009-12-09
JP2011504721A (ja) 2011-02-17
SI2152872T1 (sl) 2011-01-31
HRP20100640T1 (hr) 2010-12-31
US20090111142A1 (en) 2009-04-30
AU2008255383B2 (en) 2012-11-01
IL201395A (en) 2015-09-24
JP2015037411A (ja) 2015-02-26
PL2152872T3 (pl) 2011-03-31
AU2008255383A1 (en) 2008-12-04
ZA200908021B (en) 2010-11-24
CA2683800A1 (en) 2008-12-04
EP2152872B1 (de) 2010-09-15
PT2152872E (pt) 2010-11-17
CA2683800C (en) 2015-03-17

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