DE602008002593D1 - Verfahren zur herstellung eines rekombinanten polyklonalen proteins - Google Patents

Verfahren zur herstellung eines rekombinanten polyklonalen proteins

Info

Publication number
DE602008002593D1
DE602008002593D1 DE602008002593T DE602008002593T DE602008002593D1 DE 602008002593 D1 DE602008002593 D1 DE 602008002593D1 DE 602008002593 T DE602008002593 T DE 602008002593T DE 602008002593 T DE602008002593 T DE 602008002593T DE 602008002593 D1 DE602008002593 D1 DE 602008002593D1
Authority
DE
Germany
Prior art keywords
collection
cells
recombinant polyclonal
polyclonal protein
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602008002593T
Other languages
English (en)
Inventor
Lars Soegaard Nielsen
Dietmar Weilguny
Anne Bondgaard Tolstrup
Finn Wiberg
Christian Mueller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Symphogen AS
Original Assignee
Symphogen AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Symphogen AS filed Critical Symphogen AS
Publication of DE602008002593D1 publication Critical patent/DE602008002593D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Non-Volatile Memory (AREA)
DE602008002593T 2007-05-25 2008-05-21 Verfahren zur herstellung eines rekombinanten polyklonalen proteins Active DE602008002593D1 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200700764 2007-05-25
US92470807P 2007-05-29 2007-05-29
DKPA200701292 2007-09-07
US96000207P 2007-09-11 2007-09-11
PCT/DK2008/050116 WO2008145133A2 (en) 2007-05-25 2008-05-21 Method for manufacturing a recombinant polyclonal protein

Publications (1)

Publication Number Publication Date
DE602008002593D1 true DE602008002593D1 (de) 2010-10-28

Family

ID=39739965

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602008002593T Active DE602008002593D1 (de) 2007-05-25 2008-05-21 Verfahren zur herstellung eines rekombinanten polyklonalen proteins

Country Status (23)

Country Link
US (1) US7910332B2 (de)
EP (1) EP2152872B1 (de)
JP (2) JP5941616B2 (de)
KR (1) KR101555658B1 (de)
CN (1) CN101688204B (de)
AT (1) ATE481483T1 (de)
AU (1) AU2008255383B2 (de)
BR (1) BRPI0812091A2 (de)
CA (1) CA2683800C (de)
CY (1) CY1110939T1 (de)
DE (1) DE602008002593D1 (de)
DK (1) DK2152872T3 (de)
HK (1) HK1134519A1 (de)
HR (1) HRP20100640T1 (de)
IL (1) IL201395A (de)
MX (1) MX2009012526A (de)
NZ (1) NZ581316A (de)
PL (1) PL2152872T3 (de)
PT (1) PT2152872E (de)
SI (1) SI2152872T1 (de)
TW (1) TW200902548A (de)
WO (1) WO2008145133A2 (de)
ZA (1) ZA200908021B (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0406678A (pt) 2003-01-07 2005-12-20 Symphogen As Método para produzir proteìnas policlonais recombinantes
EP2121920B1 (de) * 2007-03-01 2011-08-24 Symphogen A/S Verfahren zur klonierung verwandter antikörper
ATE481483T1 (de) * 2007-05-25 2010-10-15 Symphogen As Verfahren zur herstellung eines rekombinanten polyklonalen proteins
BRPI0817079A2 (pt) * 2007-09-07 2016-10-11 Symphogen As processos para fabricação recombinante de anticorpos anti-rsv
NZ601353A (en) * 2008-02-01 2014-06-27 Chromocell Corp Cell lines and methods for making and using them
EP2098536A1 (de) 2008-03-05 2009-09-09 4-Antibody AG Isolation und Identifikation antigen- oder ligandenspezifischer Bindungsproteine
SG189793A1 (en) * 2008-04-23 2013-05-31 Symphogen As Methods for manufacturing a polyclonal protein
CA2726289A1 (en) * 2008-05-28 2009-12-23 Bayer Healthcare Llc Methods and compositions for production of recombinant protein in hbx-expressing mammalian cells
WO2010022738A1 (en) * 2008-08-29 2010-03-04 Symphogen A/S Method for cloning avian-derived antibodies
US8551733B2 (en) 2009-02-09 2013-10-08 Morphosys Ag Production of oligoclonal mixtures of immunoglobulins in single cells
CA2816520C (en) 2010-11-01 2017-11-21 Symphogen A/S Anti-her3 antibodies and compositions
AU2011329872B2 (en) 2010-11-16 2017-04-13 Excelimmune, Inc. Methods for producing recombinant proteins
CN102558353A (zh) * 2011-12-09 2012-07-11 上海应用技术学院 一种大肠杆菌NrfA基因表达蛋白多克隆抗体及其制备方法
GB2502127A (en) 2012-05-17 2013-11-20 Kymab Ltd Multivalent antibodies and in vivo methods for their production
CN104428318B (zh) 2012-05-02 2018-09-25 西福根有限公司 人源化泛her抗体组合物
JP2016514091A (ja) 2013-02-08 2016-05-19 ミスフォールディング ダイアグノスティクス， インコーポレイテッド トランスサイレチン抗体およびその使用
IL250902B (en) 2014-09-16 2022-08-01 Symphogen As Anti-met antibodies and preparations
US20170044608A1 (en) * 2015-07-17 2017-02-16 Allele Biotechnology & Pharmaceuticals, Inc. Methods of selecting antibodies and antibody fragments
KR20180096793A (ko) * 2016-01-14 2018-08-29 안톤 바우어 생산 세포주를 제조하는 방법
WO2018073393A2 (en) 2016-10-19 2018-04-26 Cellectis Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy
EA201992350A1 (ru) 2017-04-05 2020-03-23 Симфоген А/С Комбинированные лекарственные средства, нацеленные на pd-1, tim-3 и lag-3
CN112513074A (zh) 2018-06-22 2021-03-16 健玛保 生产两种或更多种不同抗体的受控混合物的方法
CN109988751A (zh) * 2019-01-15 2019-07-09 济宁学院 一种用于制备抗egfr全人源化单克隆抗体的细胞株构建方法
WO2020223573A2 (en) * 2019-04-30 2020-11-05 Gigagen, Inc. Recombinant polyclonal proteins and methods of use thereof
US20220344000A1 (en) * 2019-09-27 2022-10-27 Glaxosmithkline Intellectual Property Development Limited Method of predicting production stability of clonal cell lines

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0530216A4 (en) 1990-04-19 1993-06-30 Medimmune, Inc. Administration of monoclonal antibodies against respiratory viruses
GB9022543D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Antibody production
US6111166A (en) 1994-09-19 2000-08-29 Medarex, Incorporated Transgenic mice expressing human Fcα and β receptors
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
WO2001094571A1 (fr) * 2000-06-08 2001-12-13 Mitsubishi Pharma Corporation Vecteur d'expression permettant le criblage de cellules a haute expression de proteine de recombinaison, transformant a haute expression de proteine de recombinaison, et leur utilisation
US6849259B2 (en) 2000-06-16 2005-02-01 Symphogen A/S Polyclonal antibody composition for treating allergy
DK2314629T4 (da) * 2002-07-18 2023-02-06 Merus Nv Rekombinant produktion af blandinger af antistoffer
AU2003283995A1 (en) 2002-09-30 2004-04-19 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
CN100383244C (zh) * 2003-01-07 2008-04-23 西福根有限公司 用于生产重组多克隆蛋白质的方法
US20060275766A1 (en) 2003-01-07 2006-12-07 Haurum John S Method for manufacturing recombinant polyclonal proteins
BRPI0406678A (pt) * 2003-01-07 2005-12-20 Symphogen As Método para produzir proteìnas policlonais recombinantes
TWI333977B (en) 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
DE602005016818D1 (de) * 2004-07-20 2009-11-05 Symphogen As Verfahren zur charakterisierung einer polyklonalen zellinie
CA2574062A1 (en) * 2004-07-20 2006-01-26 Symphogen A/S Anti-rhesus d recombinant polyclonal antibody and methods of manufacture
EP1966241A2 (de) 2005-12-05 2008-09-10 Symphogen A/S Rekombinanter polyklonaler anti-orthopoxvirus-antikörper
JP2009528828A (ja) 2006-03-06 2009-08-13 シムフォゲン・アクティーゼルスカブ 消化器多核体ウイルス感染症の治療のための組み換えポリクローナル抗体
ATE481483T1 (de) * 2007-05-25 2010-10-15 Symphogen As Verfahren zur herstellung eines rekombinanten polyklonalen proteins

Also Published As

Publication number Publication date
KR101555658B1 (ko) 2015-09-24
MX2009012526A (es) 2009-12-09
HRP20100640T1 (hr) 2010-12-31
EP2152872A2 (de) 2010-02-17
WO2008145133A3 (en) 2009-02-05
JP2015037411A (ja) 2015-02-26
US7910332B2 (en) 2011-03-22
CN101688204B (zh) 2013-07-03
PT2152872E (pt) 2010-11-17
KR20100017923A (ko) 2010-02-16
JP2011504721A (ja) 2011-02-17
HK1134519A1 (en) 2010-04-30
ATE481483T1 (de) 2010-10-15
WO2008145133A2 (en) 2008-12-04
AU2008255383A1 (en) 2008-12-04
IL201395A (en) 2015-09-24
CA2683800C (en) 2015-03-17
EP2152872B1 (de) 2010-09-15
CY1110939T1 (el) 2015-06-10
BRPI0812091A2 (pt) 2014-10-14
SI2152872T1 (sl) 2011-01-31
ZA200908021B (en) 2010-11-24
AU2008255383B2 (en) 2012-11-01
US20090111142A1 (en) 2009-04-30
JP5941616B2 (ja) 2016-06-29
CN101688204A (zh) 2010-03-31
IL201395A0 (en) 2011-08-01
TW200902548A (en) 2009-01-16
NZ581316A (en) 2012-02-24
PL2152872T3 (pl) 2011-03-31
CA2683800A1 (en) 2008-12-04
DK2152872T3 (da) 2010-12-06

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