MX2009012526A - Metodo para fabricar una proteina policlonal recombinante. - Google Patents

Metodo para fabricar una proteina policlonal recombinante.

Info

Publication number
MX2009012526A
MX2009012526A MX2009012526A MX2009012526A MX2009012526A MX 2009012526 A MX2009012526 A MX 2009012526A MX 2009012526 A MX2009012526 A MX 2009012526A MX 2009012526 A MX2009012526 A MX 2009012526A MX 2009012526 A MX2009012526 A MX 2009012526A
Authority
MX
Mexico
Prior art keywords
collection
cells
manufacturing
recombinant polyclonal
polyclonal protein
Prior art date
Application number
MX2009012526A
Other languages
English (en)
Inventor
Anne Bondgaard Tolstrup
Lars Soegaard Nielsen
Dietmar Weilguny
Finn Wiberg
Christian Mueller
Original Assignee
Symphogen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Symphogen As filed Critical Symphogen As
Publication of MX2009012526A publication Critical patent/MX2009012526A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Non-Volatile Memory (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La invención se refiere a un método para fabricar una composición de proteína policlonal recombinante, en particular una composición de anticuerpo policlonal recombinante. El método comprende la obtención de una colección de células transfectadas con una colección de secuencias variantes de ácido nucleico, en donde cada célula en la colección es transfectada con y capaz de expresar un miembro de la colección, que codifica para un miembro distinto de una proteína policlonal. Las células son cultivadas bajo condiciones adecuadas para la expresión de la proteína policlonal, que es obtenida a partir de las células o del sobrenadante de cultivo. La secuencia de ácido nucleico es introducida dentro de las células mediante transfección con una colección de vectores. El presente método es adecuado para la fabricación de anticuerpos policlonales para usos terapéuticos.
MX2009012526A 2007-05-25 2008-05-21 Metodo para fabricar una proteina policlonal recombinante. MX2009012526A (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200700764 2007-05-25
US92470807P 2007-05-29 2007-05-29
DKPA200701292 2007-09-07
US96000207P 2007-09-11 2007-09-11
PCT/DK2008/050116 WO2008145133A2 (en) 2007-05-25 2008-05-21 Method for manufacturing a recombinant polyclonal protein

Publications (1)

Publication Number Publication Date
MX2009012526A true MX2009012526A (es) 2009-12-09

Family

ID=39739965

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009012526A MX2009012526A (es) 2007-05-25 2008-05-21 Metodo para fabricar una proteina policlonal recombinante.

Country Status (23)

Country Link
US (1) US7910332B2 (es)
EP (1) EP2152872B1 (es)
JP (2) JP5941616B2 (es)
KR (1) KR101555658B1 (es)
CN (1) CN101688204B (es)
AT (1) ATE481483T1 (es)
AU (1) AU2008255383B2 (es)
BR (1) BRPI0812091A2 (es)
CA (1) CA2683800C (es)
CY (1) CY1110939T1 (es)
DE (1) DE602008002593D1 (es)
DK (1) DK2152872T3 (es)
HK (1) HK1134519A1 (es)
HR (1) HRP20100640T1 (es)
IL (1) IL201395A (es)
MX (1) MX2009012526A (es)
NZ (1) NZ581316A (es)
PL (1) PL2152872T3 (es)
PT (1) PT2152872E (es)
SI (1) SI2152872T1 (es)
TW (1) TW200902548A (es)
WO (1) WO2008145133A2 (es)
ZA (1) ZA200908021B (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI1583830T1 (sl) 2003-01-07 2006-12-31 Symphogen As Postopek za pripravo rekombinantnih poliklonskih proteinov
EP2121920B1 (en) * 2007-03-01 2011-08-24 Symphogen A/S Method for cloning cognate antibodies
SI2152872T1 (sl) * 2007-05-25 2011-01-31 Symphogen As Postopek za pripravo rekombinantnega poliklonskega proteina
MX2010002044A (es) * 2007-09-07 2010-03-18 Symphogen As Metodos para produccion recombinante de anticuerpos anti-virus sincitial respiratorio (anti-rsv).
KR20100122491A (ko) * 2008-02-01 2010-11-22 크로모셀 코포레이션 세포주 및 이의 제조 및 사용 방법
EP2098536A1 (en) 2008-03-05 2009-09-09 4-Antibody AG Isolation and identification of antigen- or ligand-specific binding proteins
CN102007146A (zh) * 2008-04-23 2011-04-06 西福根有限公司 用于制备多克隆蛋白质的方法
CN102112622B (zh) * 2008-05-28 2014-12-10 拜耳医药保健有限公司 用于在表达HBx的哺乳动物细胞中生产重组蛋白的方法和组合物
BRPI0917352A2 (pt) * 2008-08-29 2017-08-22 Symphogen As Métodos para produção de uma biblioteca de pares cognatos que compreende sequências ligadas que codificam regiões variáveis e para gerar um vetor que codifica um anticorpo quimérico com regiões de seres humanos constantes e regiões variáveis não humanas, biblioteca de vetores, e método para produção de uma biblioteca de sequências de imunoglobulina derivadas de aves
DK2393927T3 (en) 2009-02-09 2015-03-30 Morphosys Ag Preparation of oligoclonal mixtures of antibodies in the individual cells
JP6186277B2 (ja) 2010-11-01 2017-08-23 シムフォゲン・アクティーゼルスカブＳｙｍｐｈｏｇｅｎ Ａ／Ｓ 抗ｈｅｒ３抗体および組成物
JP6092782B2 (ja) * 2010-11-16 2017-03-08 エクセリミューン， インコーポレイテッド 組換えタンパク質の製造方法
CN102558353A (zh) * 2011-12-09 2012-07-11 上海应用技术学院 一种大肠杆菌NrfA基因表达蛋白多克隆抗体及其制备方法
GB2502127A (en) 2012-05-17 2013-11-20 Kymab Ltd Multivalent antibodies and in vivo methods for their production
AU2013255537B2 (en) 2012-05-02 2018-02-15 Symphogen A/S Humanized pan-her antibody compositions
EP2953970A4 (en) 2013-02-08 2016-06-29 Misfolding Diagnostics Inc TRANSTYRETIN ANTIBODIES AND USES THEREOF
IL250902B (en) 2014-09-16 2022-08-01 Symphogen As Anti-met antibodies and preparations
US20170044608A1 (en) * 2015-07-17 2017-02-16 Allele Biotechnology & Pharmaceuticals, Inc. Methods of selecting antibodies and antibody fragments
KR20180096793A (ko) * 2016-01-14 2018-08-29 안톤 바우어 생산 세포주를 제조하는 방법
WO2018073393A2 (en) 2016-10-19 2018-04-26 Cellectis Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy
JP2020513009A (ja) 2017-04-05 2020-04-30 シムフォゲン・アクティーゼルスカブＳｙｍｐｈｏｇｅｎ Ａ／Ｓ Ｐｄ−１、ｔｉｍ−３、およびｌａｇ−３を標的とする併用治療
WO2019243626A1 (en) 2018-06-22 2019-12-26 Genmab A/S Method for producing a controlled mixture of two or more different antibodies
CN109988751A (zh) * 2019-01-15 2019-07-09 济宁学院 一种用于制备抗egfr全人源化单克隆抗体的细胞株构建方法
EP3962941A4 (en) * 2019-04-30 2023-05-10 Gigagen, Inc. RECOMBINANT POLYCLONAL PROTEINS AND METHODS OF USE THEREOF
US20220344000A1 (en) * 2019-09-27 2022-10-27 Glaxosmithkline Intellectual Property Development Limited Method of predicting production stability of clonal cell lines

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016074A1 (en) 1990-04-19 1991-10-31 Medimmune, Inc. Administration of monoclonal antibodies against respiratory viruses
GB9022543D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Antibody production
US6111166A (en) 1994-09-19 2000-08-29 Medarex, Incorporated Transgenic mice expressing human Fcα and β receptors
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
EP1293564A4 (en) * 2000-06-08 2005-08-17 Mitsubishi Pharma Corp EXPRESSION VECTOR FOR SCREENING CELLS HIGH EXPRESSION OF RECOMBINANT PROTEINS, TRANSFORMING HIGH EXPRESSION OF A RECOMBINANT PROTEIN AND USE THEREOF
US6849259B2 (en) 2000-06-16 2005-02-01 Symphogen A/S Polyclonal antibody composition for treating allergy
JP4836451B2 (ja) * 2002-07-18 2011-12-14 メルス ベー ヴェー 抗体混合物の組換え生産
WO2004029284A2 (en) * 2002-09-30 2004-04-08 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
US20060275766A1 (en) 2003-01-07 2006-12-07 Haurum John S Method for manufacturing recombinant polyclonal proteins
SI1583830T1 (sl) * 2003-01-07 2006-12-31 Symphogen As Postopek za pripravo rekombinantnih poliklonskih proteinov
CN100383244C (zh) * 2003-01-07 2008-04-23 西福根有限公司 用于生产重组多克隆蛋白质的方法
TWI333977B (en) 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
PL1787126T3 (pl) 2004-07-20 2010-03-31 Symphogen As Sposób charakteryzowania poliklonalnej linii komórkowej
EA014182B1 (ru) * 2004-07-20 2010-10-29 Симфоген А/С КОМПОЗИЦИЯ ПОЛИКЛОНАЛЬНЫХ АНТИ-RhD АНТИТЕЛ, СПОСОБ ЕЁ ПОЛУЧЕНИЯ И ПРИМЕНЕНИЕ КОМПОЗИЦИИ
AU2006322445B2 (en) 2005-12-05 2011-05-12 Symphogen A/S Anti-orthopoxvirus recombinant polyclonal antibody
US20100040606A1 (en) 2006-03-06 2010-02-18 Symphogen A/S Recombinant polyclonal antibody for treatment of respiratory syncytial virus infections
SI2152872T1 (sl) * 2007-05-25 2011-01-31 Symphogen As Postopek za pripravo rekombinantnega poliklonskega proteina

Also Published As

Publication number Publication date
JP2015037411A (ja) 2015-02-26
DE602008002593D1 (de) 2010-10-28
SI2152872T1 (sl) 2011-01-31
BRPI0812091A2 (pt) 2014-10-14
CY1110939T1 (el) 2015-06-10
KR20100017923A (ko) 2010-02-16
DK2152872T3 (da) 2010-12-06
NZ581316A (en) 2012-02-24
JP2011504721A (ja) 2011-02-17
CA2683800A1 (en) 2008-12-04
IL201395A0 (en) 2011-08-01
PT2152872E (pt) 2010-11-17
WO2008145133A3 (en) 2009-02-05
TW200902548A (en) 2009-01-16
HK1134519A1 (en) 2010-04-30
US7910332B2 (en) 2011-03-22
AU2008255383A1 (en) 2008-12-04
KR101555658B1 (ko) 2015-09-24
EP2152872B1 (en) 2010-09-15
AU2008255383B2 (en) 2012-11-01
EP2152872A2 (en) 2010-02-17
ATE481483T1 (de) 2010-10-15
PL2152872T3 (pl) 2011-03-31
IL201395A (en) 2015-09-24
US20090111142A1 (en) 2009-04-30
WO2008145133A2 (en) 2008-12-04
HRP20100640T1 (hr) 2010-12-31
CA2683800C (en) 2015-03-17
ZA200908021B (en) 2010-11-24
CN101688204B (zh) 2013-07-03
CN101688204A (zh) 2010-03-31
JP5941616B2 (ja) 2016-06-29

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