MX2009012526A - Metodo para fabricar una proteina policlonal recombinante. - Google Patents
Metodo para fabricar una proteina policlonal recombinante.Info
- Publication number
- MX2009012526A MX2009012526A MX2009012526A MX2009012526A MX2009012526A MX 2009012526 A MX2009012526 A MX 2009012526A MX 2009012526 A MX2009012526 A MX 2009012526A MX 2009012526 A MX2009012526 A MX 2009012526A MX 2009012526 A MX2009012526 A MX 2009012526A
- Authority
- MX
- Mexico
- Prior art keywords
- collection
- cells
- manufacturing
- recombinant polyclonal
- polyclonal protein
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/081—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from DNA viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Non-Volatile Memory (AREA)
Abstract
La invención se refiere a un método para fabricar una composición de proteína policlonal recombinante, en particular una composición de anticuerpo policlonal recombinante. El método comprende la obtención de una colección de células transfectadas con una colección de secuencias variantes de ácido nucleico, en donde cada célula en la colección es transfectada con y capaz de expresar un miembro de la colección, que codifica para un miembro distinto de una proteína policlonal. Las células son cultivadas bajo condiciones adecuadas para la expresión de la proteína policlonal, que es obtenida a partir de las células o del sobrenadante de cultivo. La secuencia de ácido nucleico es introducida dentro de las células mediante transfección con una colección de vectores. El presente método es adecuado para la fabricación de anticuerpos policlonales para usos terapéuticos.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA200700764 | 2007-05-25 | ||
US92470807P | 2007-05-29 | 2007-05-29 | |
DKPA200701292 | 2007-09-07 | ||
US96000207P | 2007-09-11 | 2007-09-11 | |
PCT/DK2008/050116 WO2008145133A2 (en) | 2007-05-25 | 2008-05-21 | Method for manufacturing a recombinant polyclonal protein |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2009012526A true MX2009012526A (es) | 2009-12-09 |
Family
ID=39739965
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2009012526A MX2009012526A (es) | 2007-05-25 | 2008-05-21 | Metodo para fabricar una proteina policlonal recombinante. |
Country Status (23)
Country | Link |
---|---|
US (1) | US7910332B2 (es) |
EP (1) | EP2152872B1 (es) |
JP (2) | JP5941616B2 (es) |
KR (1) | KR101555658B1 (es) |
CN (1) | CN101688204B (es) |
AT (1) | ATE481483T1 (es) |
AU (1) | AU2008255383B2 (es) |
BR (1) | BRPI0812091A2 (es) |
CA (1) | CA2683800C (es) |
CY (1) | CY1110939T1 (es) |
DE (1) | DE602008002593D1 (es) |
DK (1) | DK2152872T3 (es) |
HK (1) | HK1134519A1 (es) |
HR (1) | HRP20100640T1 (es) |
IL (1) | IL201395A (es) |
MX (1) | MX2009012526A (es) |
NZ (1) | NZ581316A (es) |
PL (1) | PL2152872T3 (es) |
PT (1) | PT2152872E (es) |
SI (1) | SI2152872T1 (es) |
TW (1) | TW200902548A (es) |
WO (1) | WO2008145133A2 (es) |
ZA (1) | ZA200908021B (es) |
Families Citing this family (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4589914B2 (ja) | 2003-01-07 | 2010-12-01 | シムフォゲン・アクティーゼルスカブ | 組換え型ポリクローナルタンパク質の製造方法 |
KR20090118993A (ko) * | 2007-03-01 | 2009-11-18 | 심포젠 에이/에스 | 동족체 항체 클로닝 방법 |
NZ581316A (en) * | 2007-05-25 | 2012-02-24 | Symphogen As | Method for manufacturing a recombinant polyclonal protein |
BRPI0817079A2 (pt) * | 2007-09-07 | 2016-10-11 | Symphogen As | processos para fabricação recombinante de anticorpos anti-rsv |
US20110003711A1 (en) * | 2008-02-01 | 2011-01-06 | Chromocell Corporation | Cell lines expressing gaba receptor and methods using them |
EP2098536A1 (en) | 2008-03-05 | 2009-09-09 | 4-Antibody AG | Isolation and identification of antigen- or ligand-specific binding proteins |
EP2280998A1 (en) * | 2008-04-23 | 2011-02-09 | Symphogen A/S | Methods for manufacturing a polyclonal protein |
KR20110021965A (ko) * | 2008-05-28 | 2011-03-04 | 바이엘 헬스케어 엘엘씨 | HBx-발현 포유류 세포에서 재조합 단백질을 생성하기 위한 방법 및 조성물 |
CA2735020A1 (en) * | 2008-08-29 | 2010-03-04 | Symphogen A/S | Method for cloning avian-derived antibodies |
ES2533952T3 (es) | 2009-02-09 | 2015-04-16 | Morphosys Ag | Producción de mezclas oligoclonales de inmunoglobulinas en células individuales |
CA2816520C (en) | 2010-11-01 | 2017-11-21 | Symphogen A/S | Anti-her3 antibodies and compositions |
DK2640743T3 (en) * | 2010-11-16 | 2017-01-23 | Excelimmune Inc | PROCEDURES FOR THE PREPARATION OF RECOMBINANT PROTEINS |
CN102558353A (zh) * | 2011-12-09 | 2012-07-11 | 上海应用技术学院 | 一种大肠杆菌NrfA基因表达蛋白多克隆抗体及其制备方法 |
GB2502127A (en) | 2012-05-17 | 2013-11-20 | Kymab Ltd | Multivalent antibodies and in vivo methods for their production |
JP6325527B2 (ja) | 2012-05-02 | 2018-05-16 | シムフォゲン・アクティーゼルスカブSymphogen A/S | ヒト化pan−her抗体組成物 |
EP2953970A4 (en) | 2013-02-08 | 2016-06-29 | Misfolding Diagnostics Inc | TRANSTYRETIN ANTIBODIES AND USES THEREOF |
BR112017005202A2 (pt) | 2014-09-16 | 2017-12-12 | Symphogen As | anticorpos anti-met e composições |
US20170044608A1 (en) * | 2015-07-17 | 2017-02-16 | Allele Biotechnology & Pharmaceuticals, Inc. | Methods of selecting antibodies and antibody fragments |
JP2019501658A (ja) * | 2016-01-14 | 2019-01-24 | アントン バウアーAnton Bauer | 生産用細胞株を作製する方法 |
WO2018073393A2 (en) | 2016-10-19 | 2018-04-26 | Cellectis | Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy |
MA49042A (fr) | 2017-04-05 | 2020-02-12 | Symphogen As | Polythérapies ciblant pd-1, tim-3 et lag-3 |
WO2019243626A1 (en) | 2018-06-22 | 2019-12-26 | Genmab A/S | Method for producing a controlled mixture of two or more different antibodies |
EP3877399A4 (en) | 2018-11-06 | 2022-10-19 | Alsatech, Inc. | CELL-BASED GENE THERAPY FOR NEURODEGENERATIVE DISEASES |
CN109988751A (zh) * | 2019-01-15 | 2019-07-09 | 济宁学院 | 一种用于制备抗egfr全人源化单克隆抗体的细胞株构建方法 |
WO2020223573A2 (en) * | 2019-04-30 | 2020-11-05 | Gigagen, Inc. | Recombinant polyclonal proteins and methods of use thereof |
JP2022550083A (ja) * | 2019-09-27 | 2022-11-30 | グラクソスミスクライン、インテレクチュアル、プロパティー、ディベロップメント、リミテッド | クローン細胞株の生産安定性を予測する方法 |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991016074A1 (en) | 1990-04-19 | 1991-10-31 | Medimmune, Inc. | Administration of monoclonal antibodies against respiratory viruses |
GB9022543D0 (en) * | 1990-10-17 | 1990-11-28 | Wellcome Found | Antibody production |
US6111166A (en) | 1994-09-19 | 2000-08-29 | Medarex, Incorporated | Transgenic mice expressing human Fcα and β receptors |
GB9703369D0 (en) | 1997-02-18 | 1997-04-09 | Lindqvist Bjorn H | Process |
EP1293564A4 (en) * | 2000-06-08 | 2005-08-17 | Mitsubishi Pharma Corp | EXPRESSION VECTOR FOR SCREENING CELLS HIGH EXPRESSION OF RECOMBINANT PROTEINS, TRANSFORMING HIGH EXPRESSION OF A RECOMBINANT PROTEIN AND USE THEREOF |
US6849259B2 (en) * | 2000-06-16 | 2005-02-01 | Symphogen A/S | Polyclonal antibody composition for treating allergy |
PT2314629E (pt) * | 2002-07-18 | 2014-01-22 | Merus B V | Produção recombinante de misturas de anticorpos |
AU2003283995A1 (en) * | 2002-09-30 | 2004-04-19 | Protein Design Labs, Inc. | Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes |
US20060275766A1 (en) * | 2003-01-07 | 2006-12-07 | Haurum John S | Method for manufacturing recombinant polyclonal proteins |
CN100383244C (zh) * | 2003-01-07 | 2008-04-23 | 西福根有限公司 | 用于生产重组多克隆蛋白质的方法 |
JP4589914B2 (ja) * | 2003-01-07 | 2010-12-01 | シムフォゲン・アクティーゼルスカブ | 組換え型ポリクローナルタンパク質の製造方法 |
TWI333977B (en) | 2003-09-18 | 2010-12-01 | Symphogen As | Method for linking sequences of interest |
EP1787126B1 (en) * | 2004-07-20 | 2009-09-23 | Symphogen A/S | A procedure for characterization of a polyclonal cell line |
CA2574062A1 (en) * | 2004-07-20 | 2006-01-26 | Symphogen A/S | Anti-rhesus d recombinant polyclonal antibody and methods of manufacture |
JP2009518320A (ja) | 2005-12-05 | 2009-05-07 | シュムフォウエン アクティーゼルスカブ | 抗オルトポックスウイルス組換えポリクローナル抗体 |
KR20080113223A (ko) | 2006-03-06 | 2008-12-29 | 심포젠 에이/에스 | 호흡기세포 융합 바이러스 감염 치료용 재조합 폴리클로날 항체 |
NZ581316A (en) * | 2007-05-25 | 2012-02-24 | Symphogen As | Method for manufacturing a recombinant polyclonal protein |
-
2008
- 2008-05-21 NZ NZ581316A patent/NZ581316A/en not_active IP Right Cessation
- 2008-05-21 MX MX2009012526A patent/MX2009012526A/es active IP Right Grant
- 2008-05-21 DE DE602008002593T patent/DE602008002593D1/de active Active
- 2008-05-21 BR BRPI0812091-9A2A patent/BRPI0812091A2/pt not_active Application Discontinuation
- 2008-05-21 CN CN2008800174383A patent/CN101688204B/zh not_active Expired - Fee Related
- 2008-05-21 KR KR1020097027038A patent/KR101555658B1/ko active IP Right Grant
- 2008-05-21 JP JP2010509679A patent/JP5941616B2/ja not_active Expired - Fee Related
- 2008-05-21 DK DK08748815.1T patent/DK2152872T3/da active
- 2008-05-21 SI SI200830107T patent/SI2152872T1/sl unknown
- 2008-05-21 CA CA2683800A patent/CA2683800C/en not_active Expired - Fee Related
- 2008-05-21 PL PL08748815T patent/PL2152872T3/pl unknown
- 2008-05-21 EP EP08748815A patent/EP2152872B1/en not_active Not-in-force
- 2008-05-21 AT AT08748815T patent/ATE481483T1/de active
- 2008-05-21 PT PT08748815T patent/PT2152872E/pt unknown
- 2008-05-21 AU AU2008255383A patent/AU2008255383B2/en not_active Ceased
- 2008-05-21 WO PCT/DK2008/050116 patent/WO2008145133A2/en active Application Filing
- 2008-05-23 TW TW097119118A patent/TW200902548A/zh unknown
- 2008-05-23 US US12/153,809 patent/US7910332B2/en not_active Expired - Fee Related
-
2009
- 2009-10-11 IL IL201395A patent/IL201395A/en active IP Right Grant
- 2009-11-13 ZA ZA2009/08021A patent/ZA200908021B/en unknown
-
2010
- 2010-03-09 HK HK10102454.3A patent/HK1134519A1/xx not_active IP Right Cessation
- 2010-11-24 CY CY20101101058T patent/CY1110939T1/el unknown
- 2010-11-24 HR HR20100640T patent/HRP20100640T1/hr unknown
-
2014
- 2014-09-19 JP JP2014191912A patent/JP2015037411A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
WO2008145133A2 (en) | 2008-12-04 |
US20090111142A1 (en) | 2009-04-30 |
ZA200908021B (en) | 2010-11-24 |
CN101688204B (zh) | 2013-07-03 |
JP5941616B2 (ja) | 2016-06-29 |
CA2683800C (en) | 2015-03-17 |
US7910332B2 (en) | 2011-03-22 |
AU2008255383A1 (en) | 2008-12-04 |
HRP20100640T1 (hr) | 2010-12-31 |
SI2152872T1 (sl) | 2011-01-31 |
DE602008002593D1 (de) | 2010-10-28 |
IL201395A (en) | 2015-09-24 |
WO2008145133A3 (en) | 2009-02-05 |
ATE481483T1 (de) | 2010-10-15 |
TW200902548A (en) | 2009-01-16 |
JP2011504721A (ja) | 2011-02-17 |
CY1110939T1 (el) | 2015-06-10 |
HK1134519A1 (en) | 2010-04-30 |
EP2152872B1 (en) | 2010-09-15 |
DK2152872T3 (da) | 2010-12-06 |
PL2152872T3 (pl) | 2011-03-31 |
CN101688204A (zh) | 2010-03-31 |
JP2015037411A (ja) | 2015-02-26 |
AU2008255383B2 (en) | 2012-11-01 |
KR20100017923A (ko) | 2010-02-16 |
IL201395A0 (en) | 2011-08-01 |
NZ581316A (en) | 2012-02-24 |
CA2683800A1 (en) | 2008-12-04 |
EP2152872A2 (en) | 2010-02-17 |
KR101555658B1 (ko) | 2015-09-24 |
BRPI0812091A2 (pt) | 2014-10-14 |
PT2152872E (pt) | 2010-11-17 |
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